JPH09206371A - Separation method for blood components - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a separation method for blood components to obtain plasma without a leucocyte and concentrated erythrocytes. SOLUTION: A parent bag comprises a first container element, a second container element and a guide tube means part. The first container element 101 is filled with the blood plasma and the second container element with the erythrocytes by centrifuge. When each blood component is transported to each child bag, the parent bag is folded in the guide tube means part and the upper layer containing plasma and the lower layer containing erythrocytes are simultaneously transported and the intermediate layer containing leucocyte is remained in the part of guide tube means.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for separating each blood component in whole blood using a blood bag.

[0002]

2. Description of the Related Art A blood bag set is used to extract various blood components contained in whole blood to obtain a blood product. FIG. 3 is a plan view showing a blood bag set which has been conventionally used. The conventional blood separation method using the blood bag set is as follows.

Blood collection needle 3 and blood collection tube 2 are used to collect whole blood in a parent bag 1 provided with two or three connection parts 9 and other connection parts, and the whole blood is collected into an upper layer containing plasma and white blood cells. After centrifuging into an intermediate layer containing (hereinafter, referred to as buffy coat) and a lower layer containing red blood cells, the communication piece 14 is brought into a communication state, and the main body 8 is pressed while the connection portion 12 is pressed through the tube 16 through the probe 16. The upper layer of blood, namely plasma, is transferred to 4.

Next, the communication piece 15 is brought into a communication state, and the buffy coat is transferred from the connection portion 13 to the child bag 5 through the tube 17 while pressing the main body 8. As a result, a child bag containing the plasma portion and a child bag containing the buffy coat are obtained. At this time, a concentrated red blood cell liquid (hereinafter referred to as CRC) remains in the parent bag 1. It is freely chosen to transfer it again to the child bag via the connection. In a recent example, a red blood cell preservation solution is added to CRC. Addition method related to this is a small child bag (not shown)
In some cases, the erythrocyte preservative solution was added to the CRC through the tube 17 by preparing.

The thus obtained product is used as a blood product. At that time, blood is transfused to the patient from the port 11 with the protector 10 provided in the bag.

[0006]

The conventional blood separation method as described above has the following problems. (1) Even after the buffy coat is transferred, a part of the inner surface of the main body 8 is soiled with the buffy coat. (2) When adding the red blood cell preservative solution to the CRC, the red blood cell preservative solution containing the buffy coat adhering to and remaining in the tube 17 may be added to the CRC, depending on the connecting method.

When the CRC is obtained in the states of (1) and (2), only a small amount of impure CRC containing the buffy coat can be obtained. Blood transfusion of such an impure CRC into the human body causes the following serious problems as a side effect.

(I) Non-hemolytic transfusion side effects (fever / post-transfusion pulmonary symptoms) occur with the production of alloantibodies (anti-HLA). (Ii) by lymphocytes in white blood cells, especially T cells,
Graft-versus-host disease (GVHD) develops and most often results in death. (Iii) White blood cells are cytomegalovirus (CM
V) and HTLV-I are transferors of viruses such as transfusion virus infection.

The present invention was invented to obtain a CRC containing no buffy coat in order to obviate the serious problems (i), (ii) and (iii).

[0010]

SUMMARY OF THE INVENTION The present invention provides a first container element and a second container element, conduit means for fluidly connecting the bottom of the first container element and the top of the second container element, and blood. The first container element and the second container element have the same relationship between the volume of the red blood cell component and the volume of the plasma component of the whole blood. Using a parent bag having a certain volume relationship such that the parent bag can be filled with whole blood and the first container element can be filled with low specific gravity and the second container element with high specific gravity. Centrifuge, fill the first container element with plasma,
The second container element is filled with red blood cells such that the surface separating the plasma and red blood cells is located in the conduit means, and an intermediate layer containing white blood cells is located in the conduit means portion, and each blood component is associated with each connection. When transferring to each child bag via the parent bag, first, the parent bag is folded at the part of the conduit means, plasma and erythrocytes are transferred at the same time, and the intermediate layer containing white blood cells is left in the part of the conduit means of the parent bag. The gist is the method for separating blood components.

The blood bag set including the container used in the present invention is preferably made of transparent or translucent synthetic resin. For example, the parent bag and the child bag are composed of two sheets made of synthetic resin such as polyvinyl chloride, and the peripheries of these sheets are bonded by heat fusion.

In the parent bag of the present invention, the first container element and the second container element have a volume relationship which is the same as the relationship between the volume of the red blood cell component and the volume of the plasma component of whole blood. The total volume of the parent bag is 128 ml, 25
6 ml, 512 ml, etc., of which 60% is the capacity of the first container element and 40% is the capacity of the second container element. In the present invention, there is a conduit means for fluidly connecting the bottom of the first container element and the top of the second container element, which requires a volume to contain the buffy coat and at which point the parent bag is folded. It is necessary. It is also preferable that a clamp can be attached at this point. Such a parent bag is disclosed in JP-A-53-38189 and U.S. Pat. No. 391.
It is known from Japanese Patent No. 1918.

At the upper end of the parent bag of the present invention, a blood collection tube is directly connected or has a connection therefor, and further a plasma transfer tube is directly connected or has a connection therefor. At the lower end of the parent bag of the present invention, a CRC transfer tube is directly connected or has a connection therefor. Others may have a blood transfusion port with a protector.

As is well known, the plasma transfer connection part and the CRC transfer connection part are made of soft tubes made of synthetic resin such as polyvinyl chloride, and adhere the peripheral edges of the two sheets of the parent bag. At that time, it is fixed by being sandwiched between both seats.

The parent bag of the present invention is used as a blood bag for collecting plasma and a bag for collecting CRC, or is mainly composed of erythrocyte preservation solution (for example, mannitol and adenine which can extend the effective period of erythrocytes to 42 days). It is usually used by being connected to a bag containing a MAP liquid) in a state of being able to communicate with a tube. Here, the state of being able to communicate means that a connecting piece (also referred to as a folding rod) is provided in the connecting portion that allows the inside and outside of the parent bag to communicate with each other, that is, the inside of the parent bag and the inside of the child bag. Such connections are well known. In addition, CPD liquid in the parent bag
A blood preservation solution such as ACD-A solution is often enclosed.

When blood is collected in the parent bag of the present invention, it is centrifuged by a well-known method to divide it into a plasma portion, a buffy coat and a CRC layer. For example, the conditions for centrifugation are usually 3000 to 4500 xg for 6 to 10 minutes, preferably 4400 xg for 6 minutes.

In transferring each blood component to each child bag via each connecting portion, in the present invention, the parent bag is first folded in two at the conduit means portion, and blood is supplied from each of the first and second container elements. The feature is that all the outlets for extracting the components are directed in the same direction upward or downward.

By folding the communicating piece and connecting the inside of the parent bag with the inside of each child bag through the connecting portion and the tube, the blood in the first and second container elements of the parent bag is pressed to thereby form the CRC. Is transferred to the CRC collection bag, and plasma is simultaneously transferred to the plasma collection bag. The transfer to this child bag is done at the same time. The buffy coat should be left as much as possible within the conduit means of the parent bag.

In the present invention, the plasma portion from the parent bag is
When each layer of the CRC part is transferred to each child bag and only the buffy coat remains in the parent bag, it may be visually controlled or optically controlled by a photo sensor or the like.

[0020]

BEST MODE FOR CARRYING OUT THE INVENTION Embodiments of the present invention will be described in detail below with reference to the drawings. FIG. 1 is a plan view showing an example of a parent bag used in the method of the present invention, and FIG. 2 is an elevation view showing another example of a parent bag used in the method of the present invention.

In FIG. 1, a first container element 101 and a second container element 102, conduit means 103 fluidly connecting the bottom of the first container element 101 and the top of the second container element 102,
It comprises means 2, 9 for introducing blood into the container elements and means (ports) 6, 7 for withdrawing blood components from each of the container elements, wherein the first container element 101 and the second container element 102 are red blood cells of whole blood. 1 shows a parent bag 1 having a volume relationship which is the same as the volume relationship between the component volume and the plasma component volume.

In FIG. 2, a conduit means 1 for fluidly connecting the first container element 101 ', the second container element 102', the bottom of the first container element 101 'and the top of the second container element 102'.
03 'and means 2', 9'for introducing blood into the container element
And means (ports) 6 ', 7'for extracting blood components from each of the container elements. The first container element 101' and the second container element 102 'are composed of the volume of red blood cell component of whole blood and the plasma component. 1 shows a parent bag 1'having a volume relationship which is the same as the volume relationship.

The parent bags 1, 1'are filled with whole blood and the first container element 101, 101 'is of low specific gravity, ie plasma, and the second container element 102, 102' is of high specific gravity, ie , The surface that divides plasma and red blood cells by centrifuging to fill the CRC is conduit means 103, 10.
3'and the buffy coat is located on the conduit means portions 103, 103 'to transfer each blood component via each connection to each child bag (not shown). First, the parent bag 1, 1'is attached to the portion 10 of the conduit means.
First, the first container element 101, 1
01 'and the second container element 102, 102' are pressed and plasma and CRC are simultaneously transferred to each child bag, and the buffy coat is part 103 of the conduit means of the parent bag 1, 1 '.
Leave in 103 '.

Blood was collected 5 times by the method of the present invention, and the blood components were centrifuged to obtain the average value of the components. As a result, 94% of the red blood cells in the parent bag 1 were CRC.
It was possible to transfer to the collection child bag, and 81% of the white blood cells existing in the parent bag 1 could be left in the parent bag 1, that is, the conduit means 103.

As a comparative example, blood was removed by the method shown in FIG.
As a result of collecting the blood repeatedly, centrifuging, and obtaining the average value of each blood component, only 88% of the red blood cells in the parent bag 1 were C
It could not be transferred to the RC collection child bag, and only 65% of the white blood cells existing in the parent bag 1 remained in the parent bag 1.

[0026]

By separating blood components by the method of the present invention,
Only by devising the squeezed condition outside the parent bag, red blood cells and plasma can pass through the area not stained by the buffy coat and be transferred to each child bag, so that CRC and plasma containing little white blood cells can be transferred. A formulation can be obtained.

Since the blood separating method of the present invention uses almost the same blood bag set as in the conventional method, almost all the steps of manufacturing the blood bag set can be followed, and the operation of obtaining a blood product can be carried out in almost the same manner as the conventional method.

In addition, in the case of adding a red blood cell preservative solution, conventionally, a method has been used in which a child bag is separately prepared and added through the preservative solution into a tube whose inner wall is soiled with a buffy coat. It is only necessary to add the red blood cell preservation solution to the child bag that stores the CRC in advance.
Thus, the operation of adding the red blood cell preservation solution is unnecessary.

[Brief description of drawings]

FIG. 1 is a plan view showing an example of a blood bag adopted in the present invention.

FIG. 2 is an elevation view showing an example of a blood bag adopted in the present invention.

FIG. 3 is a plan view of a conventional blood bag set.

[Explanation of symbols]

 1 Parent Bag 2 Blood Collection Tube 3 Blood Collection Needle 4 Plasma Collection Child Bag 5 CRC Collection Child Bag 6 Blood Component Extraction Port 7 Blood Component Extraction Port 8 Main Body 9 Connection Part 10 Protector 11 Port 12 Connection Part 13 Connection Part 14 Communication Piece 15 Communication piece 16 Tube 17 Tube 101 First container element 102 Second container element 103 Conduit means part

Claims (1)

[Claims] 1. A conduit means for fluidly connecting a first container element and a second container element, a bottom of the first container element and a top of the second container element, and means for introducing blood to the container element. Means for withdrawing a blood component from each of the container elements;
The container element and the second container element use a parent bag having a volume relationship which is the same as the relationship between the volume of the red blood cell component and the volume of the plasma component of the whole blood, and the parent bag contains whole blood. And centrifuge so that the first container element is of low specific gravity and the second container element is of high specific gravity, the first container element is filled with plasma, the second container element is filled with red blood cells, plasma The surface separating the erythrocytes and erythrocytes is located in the conduit means, and the intermediate layer containing white blood cells is located in the conduit means portion for transferring each blood component through each connection to each child bag. A method for separating blood components, which is characterized in that the parent bag is first folded at the portion of the conduit means, plasma and red blood cells are simultaneously transferred, and the intermediate layer containing white blood cells is left inside the portion of the conduit means of the parent bag.