JPH0880338A - Blood bag set - Google Patents

Abstract

PURPOSE: To obtain a thick red cell liquid without contg. white cells. CONSTITUTION: A first connecting tube 5 and a second connecting tube 11 are mounted at a master bag 4 for blood taking. A first sub-bag 8 and a second sub-bag 6 divided by branch tubes 7 are connected to this first connecting tube 5. A third sub-bag 10 is connected to this second connecting tube 11. This blood bag set is housed with a plasma component in the first sub-bag 8, a red cell preserving liquid in the second sub-bag 6 and a buffy coat in the third sub-bag 10.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a blood bag set used for separating blood into individual components.

[0002]

2. Description of the Related Art A blood bag set is used for taking out various blood components contained in whole blood and formulating them. The whole blood collected in the blood bag set is usually centrifuged to separate low-density components into upper layers and high-density components into lower layers. At that time, an intermediate layer called buffy coat containing a large amount of white blood cells can also be formed. An example of a blood bag set for extracting each of these components is shown in FIG.

The blood bag set 1 is provided with a blood collection parent bag 4 having a blood collection tube 3 to which a blood collection needle 2 is connected, and a connecting tube 5 for transferring a blood component attached to the parent bag 4. It is connected to the second child bag 6 and the first from the connecting tube 5 through the branch pipe 7.
Is connected to the child bag 8.

The connecting tube 5 is connected to a third child bag 10 via a branch pipe 9. The third child bag 10 contains a physiological saline solution containing a glucose / adenine / colloid osmotic pressure adjusting agent (mannit) developed for extending the storage period of red blood cells.

When collecting blood using such a blood bag set, first, whole blood collected from the blood donor via the blood collecting needle 2 is collected in the blood collecting parent bag 4 through the blood collecting tube 3. Since the parent bag 4 for blood collection is previously filled with a drug solution having an anticoagulant function called an ACD-A solution or a CPD solution, the collected blood is mixed with these drug solutions.

Next, the blood collection tube 3 is tightly connected in a loop shape on the side of the blood collection needle 2, cut and sealed, and separated from the blood collection needle. Furthermore, in order to avoid the coagulation of the blood remaining in the blood collection tube 3, the blood collection tube 3 is squeezed with a roller pliers or the like, and the blood in the blood collection tube 3 is moved into the parent bag 4 for blood collection so that the anticoagulation function is improved. After mixing well with the drug solution, the blood collection tube 3 that was crushed will be restored to its original shape by loosening the ironing, and the blood mixed with the drug solution will flow into the blood collection tube 3 again. To do.

The blood collected in the blood collecting parent bag 4 is
Centrifugation operation is performed according to the type of blood component to be separated, and the upper layer plasma component separated in the blood collecting parent bag 4 is transferred to the first child bag 8 and then the buffy coat layer Transferred to child bag 6.

Further, since concentrated red blood cells remain in the blood collecting parent bag 4 after the blood components have been transferred to the respective child bags, the red blood cell preservation solution contained in the third child bag 10 is It is transferred into the blood collecting parent bag 4 through the connecting tube 5 and mixed with the concentrated red blood cells. This prolongs the storage period of concentrated red blood cells.

[0009]

The above-mentioned blood bag set, when transferring the red blood cell preservation solution into the parent bag 4 for blood collection,
It will pass through the connecting tube 5. However, since the buffy coat layer containing a large amount of white blood cells has already passed through the connecting tube 5 when being transferred to the second child bag 6, the inner wall of the connecting tube 5 is not contaminated with the buffy coat. As a result, after the concentrated red blood cells remaining in the blood collecting parent bag 4 are mixed with the red blood cell preservation solution, the white blood cells attached to the inner wall of the connecting tube 5 are contained therein.

An object of the present invention is to provide a blood bag set for preventing white blood cells from being contained in the concentrated red blood cell liquid in the parent blood bag 4 for blood collection.

[0011]

The present invention relates to a blood bag set used for separating blood into individual components, wherein a blood collecting needle bag and a blood collecting tube are attached to a parent bag for collecting blood. The connection tube and the second connection tube are attached, and the first connection tube is divided into the first branch tube and the first branch tube.
Child bag and second child bag are connected, and the third child bag is connected to the second connecting tube. The plasma component is in the first child bag and the red blood cell preservation solution is in the second child bag. ,
A gist is a blood bag set, in which a buffy coat is stored in a third child bag.

[0012]

According to the present invention, when separating blood into each component, the blood components of plasma, blood preservation solution and concentrated red blood cells pass through the first connecting tube, and buffy coat passes through the second connecting tube. A blood bag set is constructed.

Therefore, when the red blood cell preservation solution is transferred into the blood collecting parent bag or when the blood component of the concentrated red blood cells is transferred into the second child bag, the connection tube (second connection) through which the buffy coat layer has passed. It does not pass through the tube) but passes through a separately provided connecting tube (first connecting tube). As a result, after the blood components of the concentrated red blood cells and the red blood cell preservative solution are mixed in the parent bag for blood collection, or in the bag containing the blood preservative solution (second child bag), the red blood cell preservative solution After mixing with the blood component of concentrated red blood cells, leukocytes are less contained in those bags.

[0014]

As described above, according to the present invention, the whole blood in the parent bag is centrifuged by the difference in density into the upper layer of plasma, the middle layer of buffy coat, and the lower layer of red blood cell containing red blood cells. When the blood product is obtained, the blood component of concentrated red blood cells having a low white blood cell content and high purity can be collected.

[0015]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described below with reference to the drawings. FIG. 1 is a plan view showing a configuration example of the blood bag set of the present invention.

In the blood bag set 1 of the embodiment,
A first child bag 8 and a second child bag 6 are connected by a connecting tube 5 to a parent bag 4 for blood collection in which whole blood is collected via a blood collection needle 2 and a blood collection tube 3, and these two child bags 6 and 8 is divided by a branch pipe 7, and a third portion for receiving a buffy coat in the blood collecting parent bag 4
Child 10 is connected by a connecting tube 11. The connecting tubes 5 and 11 are provided with a closure, commonly called a fold bar, which can be connected to each other by folding near the blood collecting parent bag 4. The whole blood of the present invention is blood collected from a human body before being subjected to a blood component separation operation.

The blood collecting parent bag 4 is for collecting whole blood, and the bag body is composed of two sheets made of thermoplastic synthetic resin such as polyvinyl chloride. It is glued on. The size of the blood collecting parent bag 4 is, for example, 15 cm in width and 20 cm in height.

When blood is collected using the blood bag set of the present invention, first, whole blood collected from the blood donor via the blood collection needle 2 is collected in the blood collecting parent bag 4 through the blood collecting tube 3. Since the parent bag 4 for blood collection is previously filled with a drug solution having an anticoagulant function called an ACD-A solution or a CPD solution, the collected blood is
It is mixed with these chemicals.

Next, the blood collection tube 3 is tightly connected in a loop shape on the side of the blood collection needle 2, cut and sealed, and separated from the blood collection needle. Furthermore, in order to avoid the coagulation of the blood remaining in the blood collection tube 3, the blood collection tube 3 is squeezed with a roller pliers or the like, and the blood in the blood collection tube 3 is moved into the parent bag 4 for blood collection so that the anticoagulation function is improved. After thoroughly mixing with the drug solution, the blood sampling tube 3 that was crushed when the ironing is loosened restores its original shape by its elasticity, and at this time, the blood mixed with the drug solution having the anticoagulant function is It flows into the blood collection tube 3 again.

The whole blood collected in the blood collecting parent bag 4 is
Put both parent and child bags in the centrifuge 44
Centrifugation was performed under the conditions of 00 × g for about 6 minutes, and in the parent bag 4 for blood collection, plasma was in the upper layer and buffy coat was in the middle portion.
Components mainly composed of red blood cells are separately formed in the lower layer. After folding the bent bar to make the inside of the first connecting tube 5 communicate with each other, the plasma component in the upper layer is transferred to the first child bag 8. At this time, a clamp or a folding bar is required between the branch pipe 7 and the second child bag 6. Next, the buffy coat layer is transferred to the third child bag 10 after folding the folding rod to connect the connecting tube 11 to the inside of the blood collecting parent bag 4.

On the other hand, the parent bag 4 for blood collection after the centrifugation operation
Although the blood component of concentrated red blood cells remains inside, the clamp (not shown) provided between the branch pipe 7 and the second child bag 6 is removed or the bent bar (not shown) between them. No.) is communicated with the second child bag 6, and the red blood cell preservative stored in the second child bag 6 is transferred into the parent bag 4 for blood collection through the communication tube 7 and mixed with the blood component of the concentrated red blood cells. It As the erythrocyte preservative, so-called MAP solution, which contains mannitol and adenine as main components, which can extend the effective period of erythrocytes to 42 days, is commonly used.

Connecting tube 11 to this third child bag
The structure which does not allow the buffy coat to pass through the communication tube 3 for the first and second child bags is an important constituent feature of the present invention. That is, in the blood bag set of the present invention, the buffy coat in the blood collecting parent bag 4 is used as the third child bag 1.
When transferring to 0, the connecting tube 11 is separately provided, and the connecting tube 5 communicating with the child bags 6 and 8 is not stained with the buffy coat. As a result, even if the red blood cell preservation solution is mixed with the blood component of the concentrated red blood cells remaining in the blood collecting parent bag 4, the white blood cells are rarely contained therein. Thus, according to the present invention, a blood product of concentrated red blood cells having a low white blood cell content can be obtained.

[Brief description of drawings]

FIG. 1 is a plan view showing a configuration example of a blood bag set of the present invention.

FIG. 2 is a plan view showing a configuration example of a conventional blood bag set.

[Explanation of symbols]

 1 blood bag set 2 blood collection needle 3 blood collection tube 4 parent bag for blood collection 5 connection tube 6 second child bag 7 branch pipe 8 first child bag 9 branch pipe 10 third child bag 11 connection tube

Claims (1)

[Claims] 1. A blood bag set used for separating blood into individual components, wherein a first collection tube and a second connection tube are provided in a blood collection parent bag to which a blood collection needle and a blood collection tube are attached. Is attached, the first child bag and the second child bag are connected to the first connecting tube by a branch pipe, and the third child bag is connected to the second connecting tube. A blood bag set comprising a first child bag, a red blood cell preservation solution contained in a second child bag, and a buffy coat contained in a third child bag.