JPH0534337A - Filter for separating leucocyte - Google Patents

Filter for separating leucocyte

Info

Publication number
JPH0534337A
JPH0534337A JP3187900A JP18790091A JPH0534337A JP H0534337 A JPH0534337 A JP H0534337A JP 3187900 A JP3187900 A JP 3187900A JP 18790091 A JP18790091 A JP 18790091A JP H0534337 A JPH0534337 A JP H0534337A
Authority
JP
Japan
Prior art keywords
filter
porous body
blood
leucocytes
open pores
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP3187900A
Other languages
Japanese (ja)
Inventor
Hitoshi Kuroki
仁 黒木
Original Assignee
Terumo Corp
テルモ株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Terumo Corp, テルモ株式会社 filed Critical Terumo Corp
Priority to JP3187900A priority Critical patent/JPH0534337A/en
Publication of JPH0534337A publication Critical patent/JPH0534337A/en
Application status is Pending legal-status Critical

Links

Abstract

PURPOSE:To separate leucocytes in blood efficiently and safely by using a porous body of a three-dimensional mesh-shaped continuous structure having continuous open pores as a filter. CONSTITUTION:A filter 1 for separating leucocytes has a construction wherein a porous body 5 formed of polyvinyl formal or polyurethane resin is supported by supporting materials 6a and 6b in a housing 4 having a blood inflow port 2 and a blood outflow port 3. The porous body 5 being the main component of the filter is constructed of a three-dimensional mesh-shaped continuous structure having continuous open pores of most frequent pore diameters 1 to 5mum. The diameters of the continuous open pores are 2 to 4mum desirably in view of clogging and a leucocyte retention rate, and the porosity rate is 75 to 95% desirably in view of the strength of the filter and a speed of separation. Since the flow passage of the filter is formed at the time when the porous body 5 is molded, nonuniformity is little and the possibility of an extraneous substance flowing out at the time of an operation is small. On the occasion of separation of the leucocytes, priming is made by a physiological saline solution and then the leucocytes out of blood constituents are retained by the porous body 5.

Description

【発明の詳細な説明】 DETAILED DESCRIPTION OF THE INVENTION

【0001】 [0001]

【産業上の利用分野】本発明は、白血球分離用フィルターに関するものである。 The present invention relates to relates to a leukocyte filter for separating. 詳しく述べると本発明は白血球に対して安定した捕捉能を示し、かつ異物の混入の恐れのない白血球分離用フィルターに関するものである。 Detailing the present invention showed stable trapping ability for leukocytes, and it relates to a risk-free leukocyte filter for separating foreign material.

【0002】 [0002]

【従来の技術】輸血の形態が従来の全血輸血から、患者が必要としている成分のみを輸血する成分輸血へと変化して久しいが、この成分輸血においては、分画した血液成分の純度をいかに高くするかが課題となっている。 BACKGROUND OF THE INVENTION transfusion forms of conventional whole blood transfusion, although long been changed to component transfusion transfusing only components which patient is in need, in this component transfusion, the purity of the fractionated blood components how high has become an issue. 従来、献血によって得られた血液は、遠心操作によって赤血球濃厚液(CRC)、濃縮血小板血漿(PC)および乏血小板血漿(PPP)に分離される。 Conventionally, blood obtained by blood donation, erythrocyte concentrates by centrifugation (CRC), is separated into platelet concentrates (PC) and platelet-poor plasma (PPP). このようにして分離された血球製剤は、赤血球あるいは血小板を必要とする患者への成分輸血に広く用いられているが、各血球製剤は、多くの白血球を含んでおり、輸血により多量の白血球が輸注されていることが問題視されている。 In this way, the separated blood cells formulations is widely used in component transfusion to a patient in need of red blood cells or platelets, each blood cell preparations are subject to a number of white blood cells, a large amount of white blood cells by blood transfusion that it is transfused it is seen as a problem. 血球製剤に含まれる白血球は、輸血後の副作用を防止する上からも極力除去する必要があり、このために従来より多くの工夫がなされている。 Leukocytes contained in blood preparations, must as much as possible to remove from the viewpoint of preventing side effects of post-transfusion, there have been many contrivances conventionally for this purpose. その方法としては、血球の比重差を利用した重力遠心分離法、白血球の粘着ないしは付着等の作用を利用した捕捉材利用の方法等が使用されている。 As the method, gravity centrifugation method using a difference in specific gravity between blood cells, and a method for capturing material utilization using an adhesive or the action of adhesion of leukocytes is used.

【0003】これらの方法の中で、捕捉材利用の方法が白血球除去効率の良さ、手技の簡便なことなどから広く用いられており、天然繊維、合成繊維等の捕捉材があるが繊維径の非常に小さな繊維をカラム内にそのまま詰めたものや不織布等に二次加工したものが多くの場合用いられている。 [0003] Among these methods, the capture material utilization methods leukocyte removal efficiency of goodness, and widely used, such as that simple procedures, natural fibers, such as capturing material but are of a fiber diameter of synthetic fiber those secondary processing is used in many cases to a very small fibers that directly packed in a column or nonwoven fabric or the like. しかしながら、この様な繊維を用いる場合においては操作中に繊維の脱落の危険性は免れず、異物流出の恐れがあった。 However, not escaped the danger of falling off of the fibers during operation in the case of using such a fiber, there is a possibility of foreign matter outflow.

【0004】 [0004]

【発明が解決しようとする問題点】したがって、本発明は新規な白血球分離用フィルターを提供することを目的とする。 [Invention is point problem to be solved] Accordingly, the present invention is an object of the present invention is to provide a novel leukocyte filter for separating. 本発明はまた、白血球に対して高くかつ安定した捕捉能を有し、血液中より効率よく白血球を分離し得る白血球分離用フィルターを提供することを目的とする。 The present invention also has a high and stable trapping ability for leukocytes, and an object thereof is to provide a leukocyte separation filter capable of separating efficiently leukocytes from blood. 本発明はさらに操作時における異物の流出の恐れがなく、安全に白血球分離操作を行ない得る白血球分離用フィルターを提供することを目的とする。 The invention further no fear of outflow of foreign matters during operation, and an object thereof is to provide a leukocyte separation filter may perform safely leukapheresis operation. 本発明はまたフィルターの製造工程を簡易なものとし、かつ製品の性能のバラツキの少ない白血球分離用フィルターを提供することを目的とする。 The present invention also made simple filter manufacturing process, and an object thereof to provide a small variation leukocyte filter for separating performance of the product.

【0005】 [0005]

【問題点を解決するための手段】上記諸目的は、最頻(最多)気孔径1〜5μmの連続開放気孔を有する三次元網目状連続組織の多孔体からなる白血球分離用フィルターにより達成される。 Means for Solving the Problems] The above various objects are achieved by a leukocyte separation filter comprising a porous body of the modal (most frequent) three-dimensional network continuous structure having a continuous open pores of pore diameter 1~5μm .

【0006】本発明は、多孔体がビニルホルマールまたはポリウレタン樹脂からなる白血球分離用フィルターである。 [0006] The present invention is a leukocyte filter for separating the porous body is made of a polyvinyl formal or polyurethane resin.

【0007】 [0007]

【作用】本発明の白血球分離用フィルターは、最頻(最多)気孔径1〜5μmの連続開放気孔を有する三次元網目状連続組織の多孔体からなることを特徴とするものである。 [Action] leukapheresis filter of the present invention is characterized by comprising a porous body modal (most frequent) three-dimensional network continuous structure having a continuous open pores of pore diameter 1 to 5 [mu] m.

【0008】この様にマトリックスが3次元網目状連続組織である上記の如き一定の範囲の気孔径を有する多孔体で血液等の白血球懸濁液を処理すると、懸濁液中に含まれる白血球は、多孔体のマトリックス間に形成される連続開放気孔よりなる複雑な流路を通過する間に効率よく捕捉されるものである。 [0008] Treatment of leukocyte suspension such as blood in a porous body having a pore size of this matrix is ​​constant above such as a 3-dimensional network continuous structure as a range, leukocytes contained in the suspension are those efficiently captured while passing through a complicated flow path consisting of continuous open pores which is formed between the matrix of the porous body. さらに、フィルターの流路は、多孔体の三次元網目状連続組織、すなわち、多孔体のマトリックスにより形成される連続開放気孔であるため、フィルターの流路は多孔体の成型時に形成されることとなるゆえ、該多孔体を用いて白血球分離フィルターを製造する際の製造工程は極めて簡易なもので、また製品の性能のバラツキは連続組織であるために安定したものであり、操作時における該多孔体からの異物の流出あるいは流路のチャンネリングなどの問題も本質的に生じないものである。 Further, the flow path of the filter, three-dimensional network continuous structure of the porous material, i.e., for a continuous open pores formed by the matrix of the porous body, that the channel filters to be formed during molding of the porous body and becomes therefore manufacturing step in manufacturing the leukocyte separation filter using a porous body in extremely simple, also is intended variation in product performance that stably for a continuous tissue, porous during operation problems such as the outflow, or channeling of the flow path of the foreign matters from the body and those that do not essentially occurs.

【0009】以下、本発明を実施態様に基づきより詳細に説明する。 [0009] Hereinafter, will be explained in more detail on the basis of the present invention embodiment.

【0010】本発明の白血球分離用フィルターは、連続開放気孔を有する三次元網目状連続組織の多孔体からなるものであり、さらに多孔体の材質がポリビニルホルマールまたはポリウレタン樹脂からなるものである。 [0010] Leukocyte separation filter of the present invention is made of a porous body of three-dimensional network continuous structure having a continuous open pores, in which further material of the porous body is made of polyvinyl formal or polyurethane resin.

【0011】本発明に関わる上記の如き構造を有する多孔体において連続開放気孔の最頻(最多)気孔径は1〜 [0011] the modal (most frequent) pore size of the continuous open pores in the porous body having the above-mentioned structure according to the present invention 1
5μm、好ましくは2〜4μmであることが望ましい。 5 [mu] m, it is desirable that preferably is 2-4 [mu] m.
すなわち、気孔径が1μm未満であると白血球除去操作時において、処理される血液あるいは白血球懸濁液中に含まれる他の血球までもが捕捉されてしまい、目詰まりを引き起こす恐れがある。 That is, when the pore diameter is less than 1μm leukocyte removal operation, even other blood cells contained in blood or leukocyte suspension is processed will be trapped and cause clogging. 一方、気孔径が5μmを越えるものであると、処理される白血球懸濁液との接触頻度が低下するために白血球の補捉率が低下してしまう恐れがあるためである。 On the other hand, when the pore diameter is one that exceeds 5 [mu] m, complement 捉率 leukocytes to frequency of contact between leukocytes suspension to be processed is lowered and there is a risk that degraded.

【0012】なお、本発明における「最頻(最多)気孔径」とは、多孔体を任意に切断し、断面全体に分散している細孔の各々について面積を測定して円に換算した直径を求め、直径と細孔の数との関係を調べたときに、円に換算した直径が最も数の多いところを表すものである。 [0012] Note that the "modal (most frequent) pore diameter" in the present invention, the porous body was arbitrarily cut and converted into yen by measuring the area for each of the pores are distributed throughout the cross-sectional diameter look, when examining the relationship between the number of diameter and pore is used to represent an Excessive-converted diameter number of most circle. すなわち、多孔体の任意の切断面に分散する細孔は色々な形で、その直径も様々であるが、個々の細孔をその細孔の断面積と同じ面積の円に換算し、その直径を1 That is, the pores dispersed in any of the cut surface of the porous body in a variety of forms, although the diameter may vary, in terms of individual pores circle having the same area as the cross-sectional area of ​​the pores, the diameter 1
μm間隔で横軸にとり、縦軸にその区間の(1μmごとの)細孔数をそれぞれとってグラフに曲線を描いたときに、その曲線のピークに当る直径が本明細書でいう最頻(最多)気孔径である。 Horizontal axis in μm intervals, and the vertical axis of the section (per 1 [mu] m) the number of pores when taking a curve in the graph respectively, the diameter hitting the peak of the curve referred to herein modal ( Most) is a pore diameter. この時細孔はランダムに2,0 At this time, the pores are randomly 2,0
00個以上数えるのが好ましい。 Preferably, the count 00 or more. したがって、最頻(最多)直径以上の細孔は数が少なくなることを表すものであって、これ以上の直径の粒子は通過しないというものではない。 Therefore, there is the modal (most frequent) diameter or more pores indicating that the number is reduced, more of the diameter of the particles not to not pass.

【0013】また、この様な多孔体の空孔率は、最頻(最多)気孔径等によっても左右されるが、75〜95 [0013] In addition, the porosity of such a porous body is also affected by the modal (most) pore diameter and the like, 75 to 95
%、より望ましくは80〜95%であることが好ましい。 %, It is preferable and more preferably 80 to 95%. すなわち、空孔率が75%以上であると白血球除去処理操作をより短時間で行うことができ、一方、空孔率が95%以下であるとフィルターとしての強度の点で優れるためである。 In other words, porosity can be carried out more quickly is the leukocyte removal process operation on more than 75%, whereas, because the porosity is excellent in terms of strength of the filter is not more than 95%. さらに、この様な多孔体の厚さは、最頻(最多)気孔径、気孔率およびマトリックスである三次元網目状連続組織の微細構造等によっても左右されるが0.1〜10mm、より望ましくは0.5〜3mm程度であることが好ましい。 Further, the thickness of such a porous body modal (most frequent) pore diameter also depends on the porosity and microstructure of the three-dimensional mesh-like continuous structure is a matrix or the like is 0.1 to 10 mm, more preferably preferably it is about 0.5 to 3 mm. すなわち、多孔体の厚さが0.1mm以上であるとフィルターの強度の点で優れ、 That is, superior in strength of the filter when the thickness of the porous material is 0.1mm or more,
10mm以下であると濾過層長が長くなり過ぎず、目詰まりの可能性が低くなるためである。 Not too long filtration layer length and is 10mm or less, because the possibility of clogging is reduced.

【0014】本発明に係わる多孔体は、所望の構造を有するものであれば、特にその材質を限定されるものではないが、血球に損傷を与えにくいものであることが望まれる。 [0014] according to the present invention the porous body, as long as it has the desired structure, but are not particularly limited in its material, is desired to be difficult to damage the blood cells. また、フィルターで処理する血液製剤等の種類により、例えば、赤血球濃厚液を処理する場合は血小板が付着し易い材質であることが望まれ、濃縮血小板血漿を処理する場合は血小板が付着しにくい材質であることが望まれる。 Also, the type of blood products utilizing treatment with a filter, for example, when processing the erythrocyte concentrates are desired to be easily made to adhere platelets, hardly adhered platelets when processing platelet concentrates Material it is desired is.

【0015】本発明に係わるポリビニルホルマールは、 The polyvinyl formal according to the present invention,
所望の構造を有するものであれば、いかなる方法によって得られたものであってもよいが、澱粉、デキストリン等のアミローズを含有する多糖類およびその誘導体、耐酸性を有するアニオン型界面活性剤、および非イオン型界面活性剤などの気孔生成剤を含有するポリビニルアルコール水溶液に、必要に応じて硫酸ナトリウム、塩化ナトリウム、硫酸アンモニウム、塩化アンモニウム、硫酸カリウム、ヨウ化ナトリウム等の無機塩を存在させ、ホルムアルデヒドおよび酸触媒を作用させてアセタール化反応を行なう溶出法(特公昭47−46455号、特公昭48−20019号)により得られたものが所望の構造を有することから特に好ましい。 As long as it has the desired structure, or may be obtained by any method, starch, polysaccharides and derivatives thereof containing amylose such as dextrin, anionic surfactants having an acid resistance, and polyvinyl alcohol aqueous solution containing a pore forming agent such as a non-ionic surfactant, sodium sulfate optionally, sodium chloride, ammonium sulfate, ammonium chloride, potassium sulfate, in the presence of inorganic salts such as sodium iodide, formaldehyde and by the action of an acid catalyst elution method for performing acetalization reaction (JP-B-47-46455, JP-B-48-20019) those obtained by is particularly preferred because it has the desired structure.

【0016】本発明に係わるポリウレタン樹脂としてはポリウレタンの合成樹脂発泡体、あるいは合成樹脂多孔質体の表面にセグメント化ポリウレタン等の血球の付着しにくい材料等をコーティングしたものが好ましい。 [0016] As the polyurethane resin according to the present invention preferably has coated synthetic resin foam polyurethane, or a synthetic resin porous material deposited hard material blood cells, such as segmented polyurethane on the surface and the like of.

【0017】本発明の白血球分離用フィルターの一実施態様を図を用いて説明する。 [0017] One embodiment of the leukapheresis filter of the present invention will be described with reference to FIG. 図1は本発明の白血球分離用フィルターの一実施態様を示す断面図である。 Figure 1 is a cross-sectional view showing one embodiment of the leukapheresis filter of the present invention. 本実施態様において白血球分離用フィルター1は、血液流入口2と血液流出口3とを備えてなるハウジング4内に上記したような構成を有するポリビニルホルマールまたはポリウレタン樹脂製の多孔体5がハウジング4の内部空間を横切って設けられているものである。 Leukocyte separation filter 1 in the present embodiment, the blood inlet 2 and a blood flow outlet 3 and the porous body 5 made of a polyvinyl formal or polyurethane resin having the structure as described above in housing 4 made comprise the housing 4 those provided across an interior space. なおこのような白血球分離用フィルター1において、多孔体5をハウジング4内に保持するために、例えば、多孔体5の前後に通液性の支持材6a 、6b を設け、該支持材6a 、6b In yet such leukocyte separation filter 1, to hold the porous body 5 in the housing 4, for example, liquid permeability of the support 6a, 6b are provided before and after the porous body 5, the supporting member 6a, 6b
により多孔体5を挟持することは任意である。 It is optional for holding the porous body 5 by. この白血球分離用フィルター1は、例えば図2に示されるような回路中に組入れられて実際に使用される。 The leukocyte separation filter 1 is actually used is incorporated in the circuit as shown in FIG. 2, for example. 図2に示される回路において、処理しようとする血液成分を入れた血液バッグ7および生理食塩水を入れた生理食塩水バッグ8が白血球分離用フィルター1より上方に位置させられ、それぞれクレンメ9a 、9b を具備してなる導液チューブ10a 、10b により白血球分離用フィルター1 In the circuit shown in FIG. 2, the saline bag 8 containing the blood bags 7 and saline were placed blood components to be processed is is positioned above the leukocyte separation filter 1, respectively clamp 9a, 9b liquid guiding tube 10a made comprises a leukocyte separation filter 1 with 10b
の血液流入口2に連通されており、一方、白血球分離用フィルター1の下方には生理食塩水回収用バッグ11と処理された血液成分を回収するための血液回収用バッグ12が位置させられ、それぞれクレンメ9c 、9d を具備してなる導液チューブ10c 、10d により白血球分離用フィルター1の血液流出口3に連通されている。 Is communicated with the blood inlet 2, while the blood collecting bag 12 for collecting blood components in the lower leukocyte separation filter 1 is treated with saline collecting bag 11 is caused to position, each clamp 9c, liquid guiding tube 10c made comprises a 9d, in communication with the blood outlet port 3 of the leukocyte separation filter 1 by 10d. 白血球分離操作はまずクレンメ9b 、9c を開き、クレンメ9a 、9d を閉じた状態で生理食塩水バッグ8より生理食塩水を白血球分離用フィルター1に流し、白血球分離用フィルター1内をプライミングする。 Leukapheresis procedure first open clamp 9b, the 9c, clamp 9a, saline from the saline bag 8 with closed 9d flowed into leukocyte separation filter 1, priming the leukocyte separation filter 1. なおプライミングに用いられた生理食塩水は生理食塩水回収バッグ1 Note saline used in the priming saline collection bag 1
0に回収される。 0 is collected in. プライミングを行なった後に今度はクレンメ9a、9d を開き、クレンメ9b 、9c を閉じて血液バッグ7より血液成分を白血球分離用フィルター1 Now clamp 9a after performing the priming, open 9d, clamp 9b, leukocyte separation filter 1 of blood component from a blood bag 7 Close 9c
に流す。 Flowing in. 白血球分離用フィルター1内において血液成分は上記のごとき構成を有する多孔体5を通過する際に該多孔体5により白血球成分を吸着捕捉され、白血球を分離されたものとなる。 Blood components in leukocyte separation filter 1 is a white blood cell component is adsorbed captured by the multi hole body 5 when passing through the porous body 5 having a configuration such as described above, and that the leukocytes are separated. このように白血球成分を除去された血液成分は連通する血液回収バッグ12に回収される。 The blood components are removed white blood cell component, as is collected in the blood collection bag 12 which communicates with. 血液バッグ7より血液成分を流し終わったなら、白血球分離用フィルター1内に残った血液を回収するために、さらにクレンメ9bを開き白血球分離用フィルター1内に再び生理食塩水を流して白血球分離用フィルター1内に残存する血液を押し出して血液回収用バッグ12 If more blood bag 7 has finished flowing blood components, in order to recover the remaining blood leukocyte separation filter 1, further for leukapheresis flowing again saline leukocyte separation filter 1 Open clamp 9b blood collection bag extruding the blood remaining in the filter 1 12
に回収し、ほぼ血液成分を回収し終えた時点でクレンメ9d を閉じクレンメ9c を開いて血液回収に用いた生理食塩水を生理食塩水回収用バッグ11内に回収して、白血球分離操作を終える。 Recovered in substantially blood components by opening the clamp 9c close the clamp 9d when you have finished collecting the physiological saline used for blood collection was collected in physiological saline collecting bag 11, completing the leukocyte separation .

【0018】 [0018]

【実施例】以下、本発明を実施例によりさらに具体的に説明する。 EXAMPLES The following more specifically describes the invention based on examples.

【0019】実施例1 最頻(最多)気孔径2〜3μm、気孔率86%のポリビニルホルマール製多孔体を厚さ1.3mm、濾過面積1 [0019] Example 1 the modal (most frequent) pore diameter 2 to 3 [mu] m, porosity of 86% of polyvinyl thickness 1.3mm the Mar made porous body, filtration area 1
00cm のものとして図1に示すような白血球分離用フィルターを作製し、さらに図2に示すような回路を用いて該白血球分離用フィルターに400ml採血して得られたCPD加ヒト赤血球濃厚液(CRC)1単位を自然落差で流した。 To produce a leukocyte separation filter as shown in FIG. 1 as being 00Cm 2, further using a circuit as shown in FIG. 2 the leukocyte separation filter 400ml blood-obtained CPD-added human erythrocyte concentrate ( CRC) was flushed with one unit in a natural fall. この結果、処理に要する時間は6分であり、また処理前後のCRC中の血球数を自動血球算定装置(Sysmex NE−6000、東亜医用電子(株) As a result, the time required for the process is 6 minutes, and an automatic blood count device blood counts during before and after the treatment CRC (Sysmex NE-6000, Toa Medical Electronics Co.
製)を用いて算定の後、液量に基づいて各血球成分の絶対数を求め、これより白血球除去率を求めたところ99 After calculated using Ltd.), the absolute number of each blood cell components on the basis of the liquid volume, was determined now to leukocyte removal rate 99
%であり、また赤血球の回収率は98%であり、さらに血小板除去率は90%であった。 A%, also 98% recovery rate of erythrocytes, the further platelet removal rate was 90%.

【0020】実施例2 最頻(最多)気孔径2〜3μm、気孔率82%のポリウレタン樹脂製多孔体を厚さ1.0mm、濾過面積50c [0020] Example 2 modal (most frequent) pore diameter 2 to 3 [mu] m, porosity of 82% with a thickness of 1.0mm the polyurethane resin porous body, filtration area 50c
のものとして図1に示すような白血球分離用フィルターを作製し、さらに図2に示すような回路を用いて該白血球分離用フィルターに200ml採血して得られたCPD加ヒト濃縮血小板血漿(PC)10単位を点滴速度(約4ml/min.)で流した。 to produce a leukocyte separation filter as shown in FIG. 1 as being m 2, and more using a circuit as shown in FIG. 2 the leukocyte separation filter 200ml blood-obtained CPD-added human platelet concentrates ( PC) was flushed with 10 units infusion rate (about 4ml / min.). この結果、滴下速度が遅くなることもなく、また処理前後のPC中の血球数を自動血球算定装置(SysmexNE−6000、東亜医用電子(株)製)とフローサイトメーター(Cyto-ACE1 As a result, no that the dropping rate becomes slow, and an automatic blood count device blood counts during before and after the treatment PC (SysmexNE-6000, manufactured by Toa Medical Electronics Co., Ltd.) as a flow cytometer (Cyto-ACE1
50、日本分光(株)製)を用いて算定の後、液量に基づいて各血球成分の絶対数を求め、これより白血球除去率を求めたところ99.9%であり、また血小板回収率は95%であった。 50, after the calculation using JASCO the Corp.), the absolute number of each blood cell components on the basis of the liquid volume, and 99.9% was determined now to leukocyte removal rate, also platelet recovery rate It was 95%.

【0021】 [0021]

【発明の効果】以上、述べたように、本発明は最頻(最多)気孔径1〜5μmの連続開放気孔を有する三次元網目状連続組織の多孔体からなる白血球分離用フィルターであるから、白血球に対して高く安定した捕捉能を有し、血液等の白血球懸濁液より白血球成分を効率よく分離し得るものであり、また、操作時における濾材の脱落による異物の混入の恐れもなく、安全に白血球除去操作を行い得るものである。 Effect of the Invention] Thus, as mentioned, since the present invention is a leukocyte separation filter comprising a porous body of three-dimensional network continuous structure having a continuous open pores of the modal (most frequent) pore diameter 1 to 5 [mu] m, has a high and stable trapping ability for leukocytes, are those capable of efficiently separating the white blood cell component from leukocyte suspension such as blood, also, without fear of contamination of foreign object by the detachment of the filter medium during operation, it is those that can be carried out safely leukocyte removal operation.

【図面の簡単な説明】 BRIEF DESCRIPTION OF THE DRAWINGS

【図1】本発明の白血球分離用フィルターの一実施態様を示す断面図である。 1 is a cross-sectional view showing one embodiment of the leukapheresis filter of the present invention.

【図2】本発明の白血球分離用フィルターの一実施態様を組込んだ血液処理回路を示す回路図である。 2 is a circuit diagram showing a blood processing circuit incorporating an embodiment of the leukapheresis filter of the present invention.

【符号の説明】 DESCRIPTION OF SYMBOLS

1…白血球分離用フィルター、2…血液流入口、3…血液流出口、4…ハウジング、5…ポリビニルホルマール多孔体、6a ,6b …支持材、7…血液バッグ、8…生理食塩水バッグ、9a ,9b ,9c ,9d …クレンメ、 1 ... leukocyte separation filter, 2 ... blood inlet, 3 ... blood outlet port, 4 ... housing, 5 ... polyvinyl formal porous material, 6a, 6b ... support, 7 ... blood bag, 8 ... saline bag, 9a , 9b, 9c, 9d ... clamp,
10a ,10b ,10c ,10d …導液チューブ、11 10a, 10b, 10c, 10d ... liquid guiding tube, 11
…生理食塩水回収用バッグ、12…血液回収用バッグ。 ... saline collecting bag, 12 ... blood collection bags.

Claims (1)

  1. 【特許請求の範囲】 【請求項1】 最頻(最多)気孔径1〜5μmの連続開放気孔を有する三次元網目状連続組織の多孔体からなる白血球分離用フィルター【請求項2】 多孔体がポリビニルホルマールからなる請求項1に記載の白血球分離用フィルター【請求項3】 多孔体がポリウレタン樹脂からなる請求項1に記載の白血球分離用フィルター [Claimed is: 1. A modal (most frequent) leukocyte separation filter 2. A consisting of a porous body of three-dimensional network continuous structure having a continuous open pores of pore diameter 1~5μm porous body leukocyte separation filter according to claim 1, the filter for leukapheresis according to claim 1 consisting of polyvinyl formal wherein the porous body is made of polyurethane resin
JP3187900A 1991-07-26 1991-07-26 Filter for separating leucocyte Pending JPH0534337A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP3187900A JPH0534337A (en) 1991-07-26 1991-07-26 Filter for separating leucocyte

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP3187900A JPH0534337A (en) 1991-07-26 1991-07-26 Filter for separating leucocyte

Publications (1)

Publication Number Publication Date
JPH0534337A true JPH0534337A (en) 1993-02-09

Family

ID=16214165

Family Applications (1)

Application Number Title Priority Date Filing Date
JP3187900A Pending JPH0534337A (en) 1991-07-26 1991-07-26 Filter for separating leucocyte

Country Status (1)

Country Link
JP (1) JPH0534337A (en)

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0630675A1 (en) * 1993-06-27 1994-12-28 Terumo Kabushiki Kaisha Leukocyte or leukocyte/platelet remover and filter therefor
US5647985A (en) * 1994-10-17 1997-07-15 Baxter International Inc. Whole blood leukodepletion and platelet filter
US5728306A (en) * 1994-12-23 1998-03-17 Baxter International Inc. Leukodepletion filter and method for filtering leukocytes from freshly drawn blood
US5972217A (en) * 1994-10-17 1999-10-26 Baxter International Inc. Blood cell separation devices having a membrane with particular coating
US6045701A (en) * 1994-10-17 2000-04-04 Baxter International Inc. Method of filtering a fluid suspension with a membrane having a particular coating
US6648922B2 (en) 1994-10-17 2003-11-18 Baxter International Inc. Method for producing improved medical devices and devices so produced
US6746482B2 (en) 1994-10-17 2004-06-08 Baxter International Inc. Method for producing medical devices and devices so produced
JP2008540108A (en) * 2005-05-20 2008-11-20 アクアポリン エーピーエス Membrane to filter water
JP2010275401A (en) * 2009-05-27 2010-12-09 Nikkiso Co Ltd Porous bead
US8999167B2 (en) 2003-08-07 2015-04-07 Asahi Kasei Medical Co., Ltd. Composite porous membrane and process for producing the same
KR101540620B1 (en) * 2014-05-19 2015-07-31 진화메디칼 주식회사 Filter
US9205432B2 (en) 2012-04-30 2015-12-08 Korea Institute Of Machinery & Materials Apparatus for self-extracting cells using magnetic field and method for self-extracting cells using the same

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0630675A1 (en) * 1993-06-27 1994-12-28 Terumo Kabushiki Kaisha Leukocyte or leukocyte/platelet remover and filter therefor
US5647985A (en) * 1994-10-17 1997-07-15 Baxter International Inc. Whole blood leukodepletion and platelet filter
US7422606B2 (en) 1994-10-17 2008-09-09 Edwards Lifesciences Corporation Medical devices and products having coatings applied thereto
US5795483A (en) * 1994-10-17 1998-08-18 Baxter International Inc. Method of separating leukocytes from blood cells using a leukodepletion filter
US6746482B2 (en) 1994-10-17 2004-06-08 Baxter International Inc. Method for producing medical devices and devices so produced
US5972217A (en) * 1994-10-17 1999-10-26 Baxter International Inc. Blood cell separation devices having a membrane with particular coating
US6045701A (en) * 1994-10-17 2000-04-04 Baxter International Inc. Method of filtering a fluid suspension with a membrane having a particular coating
US6648922B2 (en) 1994-10-17 2003-11-18 Baxter International Inc. Method for producing improved medical devices and devices so produced
US5885457A (en) * 1994-12-23 1999-03-23 Baxter International Inc. Filtration media for filtering leukocytes from freshly drawn blood
US5728306A (en) * 1994-12-23 1998-03-17 Baxter International Inc. Leukodepletion filter and method for filtering leukocytes from freshly drawn blood
US8999167B2 (en) 2003-08-07 2015-04-07 Asahi Kasei Medical Co., Ltd. Composite porous membrane and process for producing the same
JP2008540108A (en) * 2005-05-20 2008-11-20 アクアポリン エーピーエス Membrane to filter water
JP2010275401A (en) * 2009-05-27 2010-12-09 Nikkiso Co Ltd Porous bead
US9205432B2 (en) 2012-04-30 2015-12-08 Korea Institute Of Machinery & Materials Apparatus for self-extracting cells using magnetic field and method for self-extracting cells using the same
KR101540620B1 (en) * 2014-05-19 2015-07-31 진화메디칼 주식회사 Filter

Similar Documents

Publication Publication Date Title
AU617265B2 (en) Method for separating blood into blood components, and blood components separator unit
US5876611A (en) Intraoperative blood salvaging system and method
EP1279408B1 (en) Venting system
US4855063A (en) Red blood cell filtering system
EP1000651B1 (en) Leukapheretic filter medium
US4919817A (en) Blood cell washing systems and methods
US6251284B1 (en) Systems and methods which obtain a uniform targeted volume of concentrated red blood cells in diverse donor populations
ES2202491T3 (en) Filter material for removing leukocytes.
US5665233A (en) Filter apparatus for selectively removing leukocytes
JP3575620B2 (en) Continuous adjustment method and apparatus of the cell suspension
EP0329303A1 (en) Device and method for separating leukocytes from platelet concentrate
KR100557371B1 (en) System for Filtering Medical and Biological Fluids
US5762791A (en) Systems for separating high hematocrit red blood cell concentrations
US4810378A (en) Red blood cell filtering system
US6527957B1 (en) Methods for separating, collecting and storing red blood cells
AT404674B (en) System and method for editing of biological fluids
EP0829267B1 (en) Pre-storage filtration of platelets
CN1025653C (en) Device and method for the depletion of the leukocyte content of blood and blood components
US4330410A (en) Separation of leukocytes from leukocyte-containing suspension by filtration
US4246107A (en) Separation of lymphocytes from lymphocyte-containing suspension by filtration
DE69832472T2 (en) Process for obtaining hyper concentrated blood plates
US5580465A (en) Method for preparing platelets
US6361692B1 (en) On line blood processing system for obtaining for storage a blood suspension having a reduced residual leukocyte population
JP2930419B2 (en) Plasma Fe Roese apparatus and method of red blood cells
US4954251A (en) Concentric microaggregate blood filter