JPS6016587A - Automatic cultivating device - Google Patents

Automatic cultivating device

Info

Publication number
JPS6016587A
JPS6016587A JP12301883A JP12301883A JPS6016587A JP S6016587 A JPS6016587 A JP S6016587A JP 12301883 A JP12301883 A JP 12301883A JP 12301883 A JP12301883 A JP 12301883A JP S6016587 A JPS6016587 A JP S6016587A
Authority
JP
Japan
Prior art keywords
culture
cultivating
chamber
container
storage chamber
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP12301883A
Other languages
Japanese (ja)
Other versions
JPH0369508B2 (en
Inventor
Sachiko Karaki
幸子 唐木
Tokio Kano
時男 嘉納
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olympus Corp
Original Assignee
Olympus Corp
Olympus Optical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olympus Corp, Olympus Optical Co Ltd filed Critical Olympus Corp
Priority to JP12301883A priority Critical patent/JPS6016587A/en
Publication of JPS6016587A publication Critical patent/JPS6016587A/en
Publication of JPH0369508B2 publication Critical patent/JPH0369508B2/ja
Granted legal-status Critical Current

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Abstract

PURPOSE:An automatic cultivating device wherein a cultivating storage chamber for cultivating a culture by settled cultivation and an operating storage chamber for carrying out various operations are set separately, and they are connected by a communicating part capable of sending a culture, so that many disorders caused by a case where both chambers are in the same chamber are eliminated. CONSTITUTION:An automatic cultivating device consists of the operating storage chamber 21, the cultivating storage chamber 22, and the communicating part 23. The operating storage chamber 21 is equipped with the operating chamber 21a for carrying out necessary operations required for culture maintenance such as subculture operation, etc. in it, and the cultivating storage chamber 22 is provided with the cultivating chamber 22a for cultivating the operated culture under proper culture conditions in it. The communicating part 23 consists of a wall part to connect both the storage chambers 21 and 22 and to stop the outer part, and the conveyor 36 sent in it. The isolated rooms 51 and 61 are installed on the sides of the communicating part 23 in the interior of the operating storage chambers 21 and the cultivating storage chamber 22, and preferably the conveyors 54 and 64 for receiving and sending the culture medium 30 to the conveyor 36 are fixed in them, respectively.

Description

【発明の詳細な説明】 本発明は、自動培養装置、更に詳しくは、細胞等の培養
物を一定に保たれた雰囲気中で自動的に培養する自動培
養装置に関する。
DETAILED DESCRIPTION OF THE INVENTION The present invention relates to an automatic culture device, and more particularly to an automatic culture device for automatically culturing cultured products such as cells in a constant atmosphere.

周知のように、細胞等の培養物の培養技術は、医学、生
物学、薬学、農学等のあらゆる分野において、細胞レベ
ルの研児な行うために必要な基礎実験技術である。そこ
で、本出願人は、細胞等の培養物の培養を自動的1c行
うようにした自動培養装置を既に提案した(特願昭57
−39152号参照)。
As is well known, culturing techniques for cultured materials such as cells are basic experimental techniques necessary for conducting research at the cellular level in all fields such as medicine, biology, pharmacy, and agriculture. Therefore, the present applicant has already proposed an automatic culture device that automatically performs 1c culture of cultured products such as cells (Patent Application No. 57
-39152).

この従来の自動培養装置は、第1図に示すように、同装
置1の外装枠を形成する直方体状の筺体2の中央部に一
定の雰囲気(例えば、温度67℃、湿度ioo q6.
炭酸ガス濃度5チ)に保たれた培養室3が設けられてい
て、この培養室6内(C1培養容器(例えば、シャーレ
)4に収納された培養物に対して各種操作を加えるため
の種々の操作装置が付設されて構成されていた。即ち、
この自動培養装置1は、上記培養室3と、この培養室6
に上記容器4を自動的に搬入・搬出するための搬入・搬
出装置5と、搬入された容器4を所定の各操作位置に移
送する容器搬送台6と、上記容器4内から不上記容器4
に振動を加えて培養細胞(酵素処理済)を生育面から剥
離させる剥離装置9と、上記容器4中の液を攪拌したり
、他の新しい容器4に分注したりするための分注装置1
1と、新しい容器4を供給するための容器供給装置12
と、上記各種装置の動作を自動的に制御するための制御
装量13と、上記容器4中の培養物を外部から観察する
ための観察装置14とで、その主要部が構成されていた
As shown in FIG. 1, this conventional automatic culture device has a constant atmosphere (e.g., temperature 67° C., humidity ioo q6.
A culture chamber 3 maintained at a carbon dioxide concentration of 5 cm is provided, and a culture chamber 6 is provided with various operations for performing various operations on the culture stored in a C1 culture container (e.g., petri dish) 4. It was configured with an operating device attached to it.
This automatic culture device 1 includes the above-mentioned culture chamber 3 and this culture chamber 6.
a loading/unloading device 5 for automatically loading and unloading the container 4, a container transport table 6 for transporting the loaded container 4 to each predetermined operation position, and a container 4 for automatically loading and unloading the containers 4,
a peeling device 9 that peels cultured cells (enzyme-treated) from the growth surface by applying vibration; and a dispensing device 9 that stirs the liquid in the container 4 and dispenses it to another new container 4. 1
1 and a container supply device 12 for supplying new containers 4.
The main parts were composed of a control unit 13 for automatically controlling the operations of the various devices described above, and an observation device 14 for observing the culture in the container 4 from the outside.

上記搬入・搬出装置5は、上記筐体2の右側壁のほぼ中
央位置付近に、培養室6と外部とを連通するように設け
られており、簡単な操作で容器4を培養装置1内に自動
的に出し入れできるようにしている。また、この搬入・
搬出装置5は、容器4の搬入・搬出時に培養室3内の温
度や湿度が下がったり、pHが変動したり、外部汚染空
気が流入したりするのを最小限にくい止める役目もして
いる。
The loading/unloading device 5 is provided near the center of the right side wall of the casing 2 so as to communicate the culture chamber 6 with the outside, and allows the container 4 to be moved into the culture device 1 with a simple operation. It allows automatic loading and unloading. In addition, this import/
The unloading device 5 also serves to minimize the drop in temperature and humidity within the culture chamber 3, the fluctuation of pH, and the inflow of external contaminated air when the containers 4 are loaded and unloaded.

従来の自動培養装#1は、以上のように構成されており
、一定の雰囲気に保たれた培養室6内で次毘代培養系の
作成等の各種操作が行われると共に、培養物の培養が行
われるようになっていた。
The conventional automatic culture device #1 is configured as described above, and various operations such as the creation of a subculture system are performed in the culture chamber 6 maintained at a constant atmosphere, as well as the cultivation of the culture. was about to take place.

しかし、このような従来の自動培養装置1においては、
各種操作を行う操作室と、培養物の静置培養を行う培養
室6とが同−室であったため、培養室6内に配設された
各種操作装置が高温多湿(温度67℃、湿度100%)
の雰囲気に晒され、観察装置14のレンズや容器4の蓋
に水滴がN着して観察を妨げるという不具合があった。
However, in such a conventional automatic culture device 1,
Since the operation room where various operations are performed and the culture room 6 where the culture is statically cultured are in the same room, the various operation devices installed in the culture room 6 are exposed to high temperature and humidity (temperature 67°C, humidity 100°C). %)
When exposed to an atmosphere of

また、結露した水滴が雰囲気中の炭酸ガスを溶解して酸
性を呈し、これにより、各種操作装置に錆が生じて動作
不良を起こすおそれが強がった。
In addition, the condensed water droplets dissolve carbon dioxide in the atmosphere and become acidic, increasing the risk that various operating devices will rust and malfunction.

また、搬入・搬出装置5による容器4の出し入れ時や、
各種操作装置への容器や器具の供給時に、出入口や供給
口の開閉により少なからず培養室6内の空気流出が起こ
り、培養装置1の定温定湿保持機能の精度低下を招くと
いう欠点があった。
Also, when loading and unloading the container 4 by the loading/unloading device 5,
When supplying containers and instruments to various operating devices, opening and closing of entrances and exits and supply ports causes a considerable amount of air to flow out of the culture chamber 6, which has the disadvantage of causing a decrease in the accuracy of the constant temperature and constant humidity maintenance function of the culture device 1. .

さらに、1つの容器4に操作を加えるために容器搬送台
6を作動させると、静置中の他の容器4も移動させるこ
とになり、この移動によっ℃、細胞の生育面へのトJ治
を阻害したり、分裂期細胞の剥離を促進したりして、培
養物に不測の悪影響を与えるおそれがあった。
Furthermore, when the container transport platform 6 is operated in order to perform an operation on one container 4, other containers 4 that are still standing are also moved, and this movement increases the temperature and the temperature of the cell growth surface. There was a risk that this would have an unexpected adverse effect on the culture by inhibiting the healing process or promoting the detachment of mitotic cells.

本発明の目的は、上述の点に鑑み、培養物乞静置培養す
るための培養庫と、培養物に各種操作を加えるための操
作圧とを分離し、両庫の間を培養物を出入可能とする接
続部で接続するようにした自動培養装置を提供するにあ
る。
In view of the above-mentioned points, an object of the present invention is to separate a culture chamber for static culture and an operating pressure for applying various operations to the culture, and to move the culture in and out between the two chambers. An object of the present invention is to provide an automatic culture device which can be connected at a connection part that allows for connection.

以下、本発明を図示の一実施例に基づいて説明するO 第2図は、本発明の一実施例を示す自動培養装置を示し
ており、この自動培養装置は、紙面IC向かっ1左がわ
に配設された筐体でなる操作圧21と、右がわに配設さ
れた筐体でなる培養庫22と、上記操作圧21と培養庫
22との間を接続する接続部23とで、その主要部が構
成されている。上記操作圧21は、継代操作等の培養維
持に必要な操作を培養物に適時に加えるための操作室2
1aをその内部に形成しており、上記培養庫22は、操
作筒の培養物を適当な培養条件下(例えば、ヒト由来培
養物の場合には、温度37℃、湿度100%、炭酸ガス
濃度5%)で静置培養を行うための培養室22aをその
内部に形成している。上記操作圧21と培養庫22とは
、接続部26により互いに培養物が収納された容器60
を出し入れすることが可能になっており、後述するよう
に、平生は、シャッタ機構により空間的に互いに独立す
るようになっている。
Hereinafter, the present invention will be explained based on an illustrated embodiment. FIG. 2 shows an automatic culture device showing an embodiment of the present invention. An operating pressure 21 consisting of a casing disposed on the right side, a culture chamber 22 consisting of a casing disposed on the right side, and a connecting portion 23 connecting between the operation pressure 21 and the cultivation chamber 22. , its main parts are comprised. The operating pressure 21 is applied to an operating chamber 2 for timely applying operations necessary for culture maintenance such as passage operations to the culture.
1a is formed therein, and the culture chamber 22 stores the culture in the operation tube under appropriate culture conditions (for example, in the case of human-derived cultures, the temperature is 37°C, the humidity is 100%, and the carbon dioxide concentration is A culture chamber 22a for performing static culture at 5%) is formed inside the culture chamber 22a. The operating pressure 21 and the culture chamber 22 are connected to each other by a connecting portion 26 to a container 60 in which a culture is stored.
It is now possible to take them in and take them out, and as will be described later, they are made spatially independent of each other by a shutter mechanism.

上記操作圧21および培養庫22の内部には、軸31.
41によって回転自在に配設された容器搬送台54.4
4が設けられている。この容器搬送台34.44は、タ
ーンテーブル33.43を有し、ターンテーブル33 
、4’3は、操作圧21および培養庫22の内部あるい
は外部に配設された駆動源(図示せず)により回転駆動
されるようになっている。そして、ターンテーブル33
.43の外周縁部には、周方向に等間隔妃複数の載置部
IJ’32.42が固定されている。
Inside the operating pressure 21 and the culture chamber 22, there is a shaft 31.
Container conveyance table 54.4 rotatably arranged by 41
4 is provided. This container carrier 34.44 has a turntable 33.43.
, 4'3 are rotatably driven by the operating pressure 21 and a drive source (not shown) disposed inside or outside the culture chamber 22. And turntable 33
.. A plurality of mounting parts IJ' 32, 42 are fixed to the outer peripheral edge of 43 at equal intervals in the circumferential direction.

これら載置部拐32.42は、培1)r物を収納した容
器30(例えば、シャーレ)を、容易に脱落しな(・よ
うに載置するために、上面外周縁部に容器6oの底面の
外径よりも若干大きな内径を廟するリノグ状突縁32a
、42a (第6図参照)を備えていると共に、後述す
るベルトコンベア50.+50の一端部が嵌入し得るよ
うに、軸31.41から離れた外端部がゎに径方向の幅
広の切欠32b、42bが形成されている。
These mounting parts 32 and 42 are provided with a container 6o on the outer periphery of the upper surface in order to place the container 30 (for example, a petri dish) containing the culture medium 1) in such a way that it will not easily fall off. Linog-like ridge 32a having an inner diameter slightly larger than the outer diameter of the bottom surface
, 42a (see FIG. 6), as well as a belt conveyor 50., which will be described later. A wide radial notch 32b, 42b is formed at the outer end remote from the shaft 31.41 so that one end of the +50 mm can be fitted therein.

上記ベルトコンベア50.60は、操作圧21および培
養庫22の相対向する側面壁の内面がゎに取り付けられ
たハウジング51.61内に主体が配設されており、ハ
ウジング51 、61に取り付けられた支軸51a、6
1aによって中央部を回動自在に支持されている。この
ベルトコンベア50 、60は、第6図に示すよう尾、
上記支軸51a、61aに回転自在に取り付けられた横
長の杆状板体でなるフレーム52,62ト、このフレー
ム52.62の両端部に回転自在に取り付けられたプー
リ 53a 、53b ; 63a、631)と、この
プーリ53a、53L+ ; 63a、66ト間に掛は
渡された搬送ベル) 54.64とで、その主体が構成
されており、コンベア50.60の他端がゎのプーリ5
3b、63bには、更に駆動ベル) 55.65が、駆
動モータ(図示せず)の出力軸に取り付けられたブー!
J 56,66との間に掛は渡されている。なお、モー
タおよびプーリ56゜66は、フレーム52.620回
動に伴い一体的に回動する。
The belt conveyor 50.60 is mainly disposed within a housing 51.61, in which the inner surfaces of the opposing side walls of the operating pressure 21 and culture chamber 22 are attached. supporting shafts 51a, 6
The central portion is rotatably supported by 1a. The belt conveyors 50 and 60 have tails, as shown in FIG.
Frames 52, 62, which are horizontally long rod-shaped plates, are rotatably attached to the support shafts 51a, 61a, and pulleys 53a, 53b; 63a, 631 are rotatably attached to both ends of the frames 52, 62. ) and the conveyor belt (54.64), which is passed between the pulleys 53a, 53L+; 63a, 66t, and the other end of the conveyor 50.60 is
3b and 63b further include a drive bell) 55.65 is a boo! mounted on the output shaft of a drive motor (not shown).
Kake is passed between J 56 and 66. Note that the motor and pulley 56°66 rotate integrally as the frame 52.620 rotates.

上記ベルトコンベア50 、60は、ハウジング516
1に穿設された開孔51b、 61bを介して、その一
端部が上記容器搬送台34.44の載置部材32.42
の切欠32b、42bに嵌入し得る位14捷で延び出し
ており、同端部の両側部にはL字型の係合爪57,67
が先端爪部を上方に向けて固着されている。また、ベル
トコンベア5”0 、60の他端部は、接続部26に連
通ずるように操作圧21および培養庫22の相対向する
側面壁のほぼ中央部に穿設された開孔21b。
The belt conveyors 50 and 60 have a housing 516
Through the openings 51b and 61b drilled in
L-shaped engaging claws 57, 67 are provided on both sides of the same end.
is fixed with the tip claw facing upward. The other ends of the belt conveyors 5''0 and 60 are provided with an opening 21b formed approximately in the center of the opposing side walls of the operating pressure 21 and culture chamber 22 so as to communicate with the connecting portion 26.

22b K対向する位置1で延び出しており、同端部に
は連結部、1758.68が固着されている。この連結
部材58.68は、上記開孔21b、22bを介して、
後述するシャッタ24.25に固定された連結部拐24
a。
22b K extends at the opposite position 1, and a connecting part 1758.68 is fixed to the same end. This connecting member 58.68 is connected through the openings 21b and 22b,
A connecting part 24 fixed to a shutter 24, 25, which will be described later.
a.

25aに結合されている。25a.

上記ベルトコンベア50 、6[]の一端部とハウジン
グsi、6iとの間には、緊縮性のコイルばね59,6
9が介挿されており、このコイルばね59.69の弾力
によって、コンベア50.60には支軸51a、61a
の周りを反時計方向および時計方向に回動する習性が与
えられている。この習性によるコンベア50,600回
動位置は、シャッタ24.25の開閉位置に応じて規制
されるようになっており、シャッタ24.25が開孔2
1b、22bを閉成した状態(第4図(A)および(D
)参照)では、ベルトコンベア50.60の一端部は、
上記容器搬送台34.44の載置部桐32.42の下位
に対応するようになっている。また、シャッタ24.2
5が開孔21b、22bを開放した状態(第4図(B)
および(C)参照)では、:’ 7 ヘア 50,60
17)一端部は、載置部材32.42の切欠52b 、
 42b内((嵌入するようになって(・る。
Between one end of the belt conveyors 50, 6[] and the housings si, 6i, there are compressible coil springs 59, 6.
9 is inserted into the conveyor 50.60 due to the elasticity of the coil spring 59.69.
It has a habit of rotating counterclockwise and clockwise around the . The rotational position of the conveyors 50, 600 due to this behavior is regulated according to the opening/closing position of the shutter 24.25.
1b and 22b are closed (Fig. 4 (A) and (D)
), one end of the belt conveyor 50.60 is
It corresponds to the lower part of the paulownia mounting part 32.42 of the container transport table 34.44. In addition, the shutter 24.2
5 opens the holes 21b and 22b (Fig. 4(B)
and (C)):' 7 Hair 50,60
17) One end is a notch 52b of the mounting member 32.42,
42b ((begins to fit).

上記シャッタ24 、25は、上記接続部23内に上記
開孔21b、22bを外側から気密的に開閉するように
、操作圧21および培養庫22の側面壁の外面に沿って
摺動自在に配設されており、操作圧21および培養庫2
2の側面壁に固定されたソレノイド26.27によって
開閉作動されるようになっている。捷た、上記接続部2
6内には、水平状態にある上記ベルトコンベア50 、
60の上面とその上面がほぼ同一平面上に位置するよう
に、ベルトコンベア28がその両端部を上記開孔21b
、22bに対応させて配設されている。このベルトコン
ベア2Bカ、フレーム29ト、一対のプーリ35a 、
 35bと、搬送ベルト36とで、その主体を構成され
ていることは云うまでもない。
The shutters 24 and 25 are slidably arranged along the operating pressure 21 and the outer surface of the side wall of the culture chamber 22 so as to airtightly open and close the openings 21b and 22b in the connecting portion 23 from the outside. The operating pressure 21 and culture chamber 2
It is opened and closed by solenoids 26 and 27 fixed to the side walls of 2. The above connection part 2 which was cut off
6 includes the belt conveyor 50 in a horizontal state,
The belt conveyor 28 connects both ends of the belt conveyor 28 to the opening 21b so that the top surface of the belt conveyor 60 and the top surface of the belt conveyor 60 are located on substantially the same plane.
, 22b. This belt conveyor 2B, frame 29, pair of pulleys 35a,
35b and the conveyor belt 36 constitute its main body.

以上のように、本発明の自動培養装置は構成されても・
る。
As described above, even if the automatic culture device of the present invention is configured:
Ru.

次に、この自動培養装置の動作IF:、ついて説明する
Next, the operation IF of this automatic culture device will be explained.

培養物を収納した容器60ヲ、例えば操作圧21から培
養庫22に移したときには、まず、容器搬送台34のタ
ーンテーブル36を回転させて、第4図(イ)に示すよ
うに、移そうとづ−る容器30を載置する載置部材62
をベルトコンベア50の一端部と対向する位置1で移動
させる(以下、この位置を搬入・搬出位置と呼ぶ)。次
に、ソレノイド26に通電を行い、第4図(B)に示す
ように、シャッタ24を下方に摺動させて、開化21b
を開放させる。このとき、開孔21bとシャッタ24と
により規定される実質的な開放面積は、容器60の縦断
向Aftよりもわずかに太きいのみで、容器60が開孔
21bを通過するのに必要最小限の開放面積となってい
る。上記シャッタ24が下方に摺動すると、連結部相’
 24a、58を介して、ベルトコンベア50が支jt
l151aの周りをコイルばね59の弾力に抗して時計
方向に回動し、その一端部が搬入・搬出位置にある載置
部材62の切欠ろ2b内に嵌入する。この際、載置部材
52上に載置された容器60は、コンベア50の一端に
取り付げられた係合爪57によって押し上げられ、載置
部材62から浮き上げられると共に、その一端部をコン
ベア50の搬送ベルト54上に載せられる。従って、駆
動モータ(図示せず)が回転され、プーリ56.ベルト
55.ブー!j 53bを介して搬送ベルト54が矢印
aで示す方向に送られると、容器30がベルト54上に
載り、開孔51bを通過して開孔21bに向けて移動さ
れる。そして、容器60は、ベルトコンベア50の搬送
力により、既に開放している開孔21bを通過して接続
部23内に進入し、回部26内に配設されたベルトコン
ベア28の搬送力により搬送ベルト66上に載せられる
When the container 60 containing the cultured material is transferred from the operating pressure 21 to the culture chamber 22, first, rotate the turntable 36 of the container transfer table 34 and transfer it as shown in FIG. 4(A). Placement member 62 on which the container 30 is placed
is moved to position 1 facing one end of the belt conveyor 50 (hereinafter, this position will be referred to as the loading/unloading position). Next, the solenoid 26 is energized to slide the shutter 24 downward and open the opening 21b as shown in FIG. 4(B).
to be opened. At this time, the substantial open area defined by the aperture 21b and the shutter 24 is only slightly larger than the longitudinal direction Aft of the container 60, which is the minimum area necessary for the container 60 to pass through the aperture 21b. It has an open area of . When the shutter 24 slides downward, the connecting part phase'
The belt conveyor 50 is supported via 24a and 58.
It rotates clockwise around l151a against the elasticity of the coil spring 59, and one end thereof fits into the notch 2b of the mounting member 62 at the loading/unloading position. At this time, the container 60 placed on the placement member 52 is pushed up by the engaging claw 57 attached to one end of the conveyor 50, and is lifted off the placement member 62, and the one end is placed on the conveyor 50. 50 conveyor belts 54. Accordingly, a drive motor (not shown) is rotated and pulley 56. Belt 55. Boo! When the conveyor belt 54 is sent in the direction indicated by the arrow a through the j 53b, the container 30 is placed on the belt 54, passes through the aperture 51b, and is moved toward the aperture 21b. Then, the container 60 passes through the open hole 21b and enters the connecting portion 23 due to the conveying force of the belt conveyor 50, and due to the conveying force of the belt conveyor 28 disposed in the turning portion 26, It is placed on the conveyor belt 66.

次に、ソレノイド26への通電を解除すると、ベルトコ
ンベア50がコイルはね59の弾力(でよって支軸51
aの周りを反時剖方向に回動し、その一端部を載置部材
62の切欠32bに嵌入させた位置から下方に向けて退
避させる。また、ベルトコンベア50の回動&C伴い、
シャッタ24が上方に向けて摺動し、開孔21bが閉成
されて、操作圧21と接続部26とが再び気密的に隔離
される。
Next, when the solenoid 26 is de-energized, the belt conveyor 50 moves due to the elasticity of the coil spring 59 (and therefore the support shaft 51
a in the counter-chronological direction, and retreat downward from the position where one end thereof is fitted into the notch 32b of the mounting member 62. In addition, with the rotation &C of the belt conveyor 50,
The shutter 24 slides upward, the opening 21b is closed, and the operating pressure 21 and the connecting portion 26 are again airtightly isolated.

続いて、接続部26のベルトコンベア28の作動により
容器60が培養庫22の開化22bの手前まで移送され
ると、ソレノイド27に通電が行われて、第4図(C)
 K示すように、シャッタ25が下方に向けて摺動され
る。これにより、開孔22bが開放される。このときの
開孔221)とシャッタ25とにより規定される実質的
な開放面積は、容器60が開孔22bを通過するのに必
要最小限の大きさになっている。
Subsequently, when the container 60 is transferred to the front side of the opening 22b of the culture chamber 22 by the operation of the belt conveyor 28 of the connecting part 26, the solenoid 27 is energized and the state shown in FIG. 4(C) is carried out.
As shown in K, the shutter 25 is slid downward. This opens the aperture 22b. At this time, the substantial open area defined by the aperture 221) and the shutter 25 is the minimum size necessary for the container 60 to pass through the aperture 22b.

上記シャッタ25が下方に摺動すると、連結部拐25a
、 68を介して、ベルト・コンベア60が支軸61a
の周りをコイルばね69の弾力に抗して反時旧方向に回
動し、その一端が搬入・搬出立置にある載置部材42の
切欠42b内に嵌入されると共に、その他端部が開孔2
2bに対応する。よって、ベルトコンベア28の搬送力
により開孔22bヲ通過しようと培養庫22内に進入し
た容器60は、こんどはベルトコンヘア60の搬送力に
より同コンベア6oノ搬送ベルト64上に載せられ、矢
印すで示す方向に向けて移送される。このため、容器6
0は、ハウジング61の開孔61bを通過してベルトコ
ンベア6oの一端部に達し、同コンベア60の搬送力に
より爪部材67に乗り上げた状態となる。
When the shutter 25 slides downward, the connecting portion 25a
, 68, the belt conveyor 60 connects to the support shaft 61a.
is rotated in the counterclockwise direction around the coil spring 69 against the elasticity of the coil spring 69, and one end thereof is fitted into the notch 42b of the mounting member 42 in the loading/unloading vertical position, and the other end is opened. Hole 2
Corresponds to 2b. Therefore, the container 60 that has entered the culture chamber 22 to pass through the opening 22b due to the conveyance force of the belt conveyor 28 is then placed on the conveyor belt 64 of the conveyor 6o by the conveyance force of the belt conveyor 60, and is then placed on the conveyor belt 64 of the same conveyor 6o, as shown by the arrow. It will be transported in the direction shown above. For this reason, container 6
0 passes through the opening 61b of the housing 61, reaches one end of the belt conveyor 6o, and rides on the claw member 67 due to the conveying force of the conveyor 60.

次に、この状態からソレノイド27への通電を解除する
と、ベルトコンベア60がコイルばね69の弾力によっ
て支軸61aの周りを時計方向に回動し、第4図(D)
に示すように、その一端部を載置部月42の切欠421
)に嵌入させた位置から下方に向けて退避させる。この
ため、容器60は、搬入・搬出位置にある載置部材42
上に載置される。また、ベルトコンベア60の回動6て
伴い、シャッタ25が上方に向けて摺動し、開孔22b
が閉成されて、培養庫22が再び気密状態に復帰される
Next, when the power to the solenoid 27 is removed from this state, the belt conveyor 60 rotates clockwise around the support shaft 61a due to the elasticity of the coil spring 69, as shown in FIG. 4(D).
As shown in FIG.
) from the position where it was inserted. Therefore, the container 60 is placed on the mounting member 42 at the loading/unloading position.
placed on top. Further, as the belt conveyor 60 rotates 6, the shutter 25 slides upward, and the opening 22b
is closed, and the culture chamber 22 is returned to an airtight state.

このようにして、操作JiE21から培養庫22への容
器60の移送が行われるが、培養庫22から操作圧21
への容器60の移送も、上述の順次の工程な逆に辿って
ほぼ同様に行われる。よっ℃、培養庫22に静置培養中
の容器30に何か操作を加えるときには、最小の開放面
積と開放時間で操作圧21に容器60を自動的に搬入す
ることができ、目的とする操作を加えた後には、同様に
して容器60を培養庫22に自動的に戻すことができる
In this way, the container 60 is transferred from the operation JiE 21 to the culture warehouse 22, but the operation pressure 21
Transfer of the container 60 to the container 60 is also performed in substantially the same manner by following the above-described sequential steps in reverse. Therefore, when performing some operation on the container 30 undergoing static culture in the culture chamber 22, the container 60 can be automatically transferred to the operating pressure 21 with the minimum open area and opening time, and the desired operation can be carried out. After adding , the container 60 can be automatically returned to the culture chamber 22 in the same manner.

以上述べたように、本発明によれば、操作圧と培養庫と
を分け、両庫の間を接続部(Cより容器が出入自在とな
るように接続したのゼ、操作庫内に配設された各種操作
装置が高温多湿の雰囲気に晒されることがなく、また、
炭酸ガスを充満させることに伴う庫内水滴のp I−1
の低下もないので、各棟操作装置における錆の発生や動
作不良のおそれが少なくなり、装置の運転精度が飛躍的
に向上する。
As described above, according to the present invention, the operating pressure and the culture chamber are separated, and the two chambers are connected to each other through the connection part (C) so that the container can be freely entered and exited. The various operating devices that have been installed are not exposed to a high temperature and humidity atmosphere, and
p I-1 of water droplets in the refrigerator due to filling with carbon dioxide gas
Since there is no decrease in the temperature, there is less risk of rust occurring or malfunction in each ridge operating device, and the operating accuracy of the device is dramatically improved.

また、操作圧における容器−や器具の供給の際の供給口
の開閉によっても、培養庫内は気密に保たれるので、温
jW、湿度、炭酸ガス濃度等を一定に保つというインキ
ュベータ機f1ヒの精度が格段的に向上する。
In addition, the inside of the culture chamber is kept airtight by opening and closing the supply port when supplying containers and instruments at operating pressure, so the incubator machine f1 maintains constant temperature, humidity, carbon dioxide concentration, etc. The accuracy of this will be significantly improved.

さらに、操作中における容器搬送台の移動の影響が操作
対象の容器に収納された培養物にしか及ばないので、他
の静置培養中の培養物に衝撃等の悪影響が作用するおそ
れが少ない。
Furthermore, since the movement of the container carrier during operation affects only the culture stored in the container to be operated, there is little risk of adverse effects such as impact on other cultures that are being cultured statically.

さらにまた、培養庫内に各種操作装置が入り込まないの
で、培養庫内を一定雰囲気に保ち易い。
Furthermore, since various operating devices do not enter the culture chamber, it is easy to maintain a constant atmosphere inside the culture chamber.

捷た、無駄な培養庫内の空気の流出を防止することがで
きるので、温度、湿度、炭酸ガス濃度の制量が効率良く
行え、省エイ・ルギ運転が可能である。
Since it is possible to prevent wasteful and wasteful air from flowing out of the culture chamber, temperature, humidity, and carbon dioxide concentration can be controlled efficiently, allowing for energy-saving and energy-saving operation.

さらIで、培養庫には、培養物を収納した容器以外の出
入がないので、庫外からのコンタミネーション(細菌汚
染)の危険性が少ない。
Furthermore, since there is no access to the culture chamber other than the containers containing the culture, there is little risk of contamination (bacterial contamination) from outside the chamber.

よって、明細−、!J 1m頭に述べた従来の欠点を1
67消1−る、使用上甚だ便利な自動培養装置を提供す
ることができる。
Therefore, details-,! J 1mThe conventional drawbacks mentioned above are 1
Therefore, it is possible to provide an automatic culture device that is extremely convenient to use.

【図面の簡単な説明】[Brief explanation of the drawing]

第1図は、従来の自動培養装置の一例を示す正面要部断
面図、 第2図は、本発明の一実施例を示す自動培養装置の平面
要部断面図、 第3図は、上記第2図に示した自動培養装置の側面要部
断面図、 第4図(A)〜(D)は、上記第2図に示した自動培養
装置の作動態様をそれぞれ示す要部断面図である。 21・・・・・・・・操作庫 22・・・・・・・・培養庫 23・・・・・・・・接続部 24、25・・・・シャッタ 26、27・・・・ソレノイド 2B 、 50 、60・・・ベルトコンベア60・・
・・・・・・容器 32、42・・・・載置部拐 33、45・・・・ターンテーブル 34、44・・・・容器搬送台 特許出願人 オリンパス光学工業株式会社′ ; 代 理 人 藤 川 七 部゛;、°り・、1:l
FIG. 1 is a front sectional view of a main part showing an example of a conventional automatic culture device, FIG. 2 is a sectional plan view of a main part of an automatic culture device showing an embodiment of the present invention, and FIG. FIGS. 4A to 4D are sectional views of main parts of the automatic culture apparatus shown in FIG. 21......Operation cabinet 22...Cultivation cabinet 23...Connection parts 24, 25...Shutters 26, 27...Solenoid 2B , 50 , 60...belt conveyor 60...
... Containers 32, 42 ... Placement parts 33, 45 ... Turntables 34, 44 ... Container transport platform Patent applicant Olympus Optical Industry Co., Ltd.'; Agent Fujikawa Seven Parts゛;,°ri・,1:l

Claims (1)

【特許請求の範囲】 細胞等の培養物を自動的に培養して維持する培養装置に
おいて、 温度、湿度、ガス濃JW等の培養条件を維持し、培養物
を静置培養するための培養庫と、次世代培養系の作成等
の培養操作を自動的に行わせるための操作庫と、 上記培養庫と上記操作庫との間を、培養物を出入可能に
接伏する接続部と、 を具備することを%徴とする自動培養装置。
[Scope of Claims] A culture device for automatically cultivating and maintaining cultures such as cells, comprising a culture chamber for statically culturing the culture while maintaining culture conditions such as temperature, humidity, gas concentration, etc. , an operation chamber for automatically performing culture operations such as the creation of a next-generation culture system, and a connection section for connecting the culture chamber and the operation chamber so that the culture can be taken in and out. An automatic culture device that is equipped with the following features.
JP12301883A 1983-07-06 1983-07-06 Automatic cultivating device Granted JPS6016587A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP12301883A JPS6016587A (en) 1983-07-06 1983-07-06 Automatic cultivating device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP12301883A JPS6016587A (en) 1983-07-06 1983-07-06 Automatic cultivating device

Publications (2)

Publication Number Publication Date
JPS6016587A true JPS6016587A (en) 1985-01-28
JPH0369508B2 JPH0369508B2 (en) 1991-11-01

Family

ID=14850184

Family Applications (1)

Application Number Title Priority Date Filing Date
JP12301883A Granted JPS6016587A (en) 1983-07-06 1983-07-06 Automatic cultivating device

Country Status (1)

Country Link
JP (1) JPS6016587A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6423883A (en) * 1987-07-20 1989-01-26 Kirin Brewery Rotary shelf apparatus for culture vessel
JP2004016194A (en) * 2002-06-20 2004-01-22 Hitachi Medical Corp Cell culture apparatus
WO2004090093A1 (en) * 2003-04-09 2004-10-21 Olympus Corporation Culture treating device and automatic culture treating device
JP2008054647A (en) * 2006-09-04 2008-03-13 Hitachi Ltd Culture apparatus

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6423883A (en) * 1987-07-20 1989-01-26 Kirin Brewery Rotary shelf apparatus for culture vessel
JP2004016194A (en) * 2002-06-20 2004-01-22 Hitachi Medical Corp Cell culture apparatus
WO2004090093A1 (en) * 2003-04-09 2004-10-21 Olympus Corporation Culture treating device and automatic culture treating device
JP2008054647A (en) * 2006-09-04 2008-03-13 Hitachi Ltd Culture apparatus

Also Published As

Publication number Publication date
JPH0369508B2 (en) 1991-11-01

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