JPS60109518A - Production of bath agent containing enzyme - Google Patents

Abstract

PURPOSE:To obtain a bath agent having improved cleaning ability of the skin, detergency as washing water, good stability of a dyestuff and improved heat insulating effect, by incorporating a proteolytic enzyme derived from microorganisms with an enzyme from animal internal organs, a crude drug, dyestuff and perfume. CONSTITUTION:A bath agent obtained by incroporating a proteolytic enzyme, e.g. ASP protease, derived from microorganisms with an enzyme from animal internal organs, e.g. pancreatin, and a crude drug, e.g. ginseng powder, poeny root powder or cork tree bark extract, adding a dyestuff, e.g. Blue No.1 or Yellow No.4, with a dyestuff dissolving agent, e.g. propylene glycol, to the resultant mixture, and mixing a perfume therewith. The two kinds of the enzymes enhance the cleaning ability of the skin and detergency as washing water within a wide pH and temperature range, and the crude drug stabilizes the enzymes. The stabilization of the dyestuff is promoted by the crude drug and the dyestuff stabilizer. The heat insulation property is further enhanced by the overall action of the respective components.

Description

[Detailed description of the invention] The present invention relates to a method for producing enzyme-containing bath additives.

Conventionally, plant-derived proteolytic enzymes such as papain have been used alone as enzymes in enzyme-containing bath additives, but such enzymes alone cannot produce homogeneous proteins over a wide pl-1 range or a wide temperature range. In addition to being unable to maintain the resolution of fat and starch present on the skin surface, it is also difficult to cleanse the skin sufficiently due to differences in pto1 due to water quality or differences in humidity during use. It easily became a breeding ground for fresh germs (and could not prevent skin diseases. Furthermore, when leftover bath water is used as washing water, it is used at a relatively low humidity, so the enzyme activity is low and the enzyme In addition, other bath additives lack the stability of the blended pigments, which is one of the important elements for bath additives, and furthermore, the stability of the enzyme whites is poor. It's not good either,
Due to changes in the enzyme over time, the decomposition ability may decrease and the desired effect may not be achieved.Actually, the effects and efficacy of the product are skin cleansing and promotion of cleansing when leftover hot water from a bath is used as cleansing water. The effects etc. were not sufficiently obtained. In the present invention, proteolytic enzymes of microbial origin, enzymes obtained from animal organs that have proteolytic, lipolytic, and starch decomposing effects, and raw chestnut components are mixed together. It is excellent for skin cleansing in a wide temperature range and for strengthening the detergent power when using the residual thirst from the bath as washing water, and can prevent skin diseases, maintain the degrading ability of enzymes by stabilizing the enzymes themselves, and improve pigmentation. The purpose of the present invention is to provide a method for producing enzyme-containing bath additives that maintains the stability of the enzymes.

The following is a deviation from the embodiment of the present invention.

That is, the formulation of the embodiment of this invention consists of an enzyme, a sodium material, a herbal medicine powder or a fresh leaf extract, a pigment and a pigment solubilizing agent, a pigment stabilizer, and a fragrance. Alternatively, a mixture of crude drug extracts is mixed with a pigment dissolved in a pigment dissolving agent, and then fragrance 1' is added.
i1, a dye stabilizer is sequentially mixed.

The enzymes include proteolytic enzymes derived from microorganisms and enzymes obtained from animal organs that have proteolytic, lipolytic, and starch degrading actions. Examples of enzymes used include ΔSP proteavi, Mycobacterium protease, and Aspergillus Aspergillus protease, and pancreatin and the like are used as enzymes derived from animal organs.Furthermore, as the fl-rium material, t!A is used.
Use sodium oxyhydrogen and sodium chloride.

In addition, as crude drug powder or crude drug extract, Shaku A7 powder,
Uses powdered Koboku, powdered ginseng, powdered ginseng, extract of Prunus japonicus, etc.

Blue No. 1 and Yellow No. 4 are used as dyes, and propylene glycol is used as a dye solubilizer.

In addition, fatty acid ester (yodipentaerythritol) is used as a dye stabilizer.

The formulations in the above examples were designed to achieve the following effects. In other words, the two types of enzymes are formulated to maintain enhanced cleaning power when used in water (for skin cleansing and for cleaning bath residue) over a wide temperature range and a wide temperature range. The herbal medicine powder or herbal medicine extract is blended to stabilize enzymes, and the herbal medicine powder or herbal medicine extract and pigment stabilizer are blended to stabilize pigments. It was formulated to achieve this goal.

As a proof of the intended effect, the above-mentioned example was set as a specific example of four scales, and experimental data was collected for each effect.

In addition, in order to compare the detergent power of skin cleansing and washing water, pancreatin, an enzyme derived from animal organs, was removed. Control B excluding Proteapi was set up.

In addition, for comparison of enzyme stabilization and pigment stabilization, a control C was set in which crude drug powder and crude drug extract were excluded.

In addition, for comparison of dye stabilization, a control D was set up in which both herbal drug powder and herbal drug extract as well as dipentaeri 1-lit fatty acid ester as a dye stabilizer were removed, and Control D was set up in which both the herbal drug powder and herbal drug extract and dipentaeri 1-lit fatty acid ester as a dye stabilizer were removed. Set. Table 1 shows each formulation.

b) Detergent power when the remaining thirst from skin cleansing and bathing is used as washing water The decomposition power of proteins, fats, and starches in a specified temperature range and a specified PL-1 range is determined by the relative activity and residual activity of enzymes. Judgment was made by comparing.

i) Protein degradation test Plant-derived protein W? Commercial bath salts containing enzymes and 21-1
~Roll Δ and Example 1 are compared, and Hf
The fa11 method is based on [Anson-Hagiwara's modified method].
The test was carried out with one unit showing a color change corresponding to 1/11 of tyrosine per minute.

The reaction temperature and activity measurement results are shown in Table 2-1 and Graph 2-1.
Reaction p1-1 and activity measurement results are shown in Table 2-2 and Graph 2-2.
Table 2 shows the residual activity measurement results when the sample was made into an aqueous solution.
3. They are shown in graphs 2-3, and from the same data, it was found that Example 4, Control [1-L △, and commercially available bath additives] had a constant high relative activity in a wide temperature range or in a wide temperature range. It was confirmed that these compounds showed high residual activity over time in the same order.

Therefore, by comparing commercially available bath additives and Control A, f
Ill protein from animal sources! ΔSP protease, which is a proteolytic enzyme of microbial origin, or a combination of ASP protease and crude drug ingredients shows a constant high relative activity over a wide temperature range and a wide rlH range. It was proven that it showed a constant and high residual activity over time. In addition, when comparing Con 1 Herol 8 and Example 4, it was found that ASP protease combined with pancreatin, an enzyme obtained from animal organ II, had a wider humidity range and p.
It was proved that it showed a constant high relative activity at l-1taJ and a constant high residual activity over time.

11) Fat decomposition test W) Commercially available bath additives containing enzymes obtained from J animal organs;
Control B and Example 4 were compared, and the activity was measured by using olive oil as a substrate and determining the fatty acid produced by lipase action by alkaline titration, and determining the lipase activity from the value. .

The reaction temperature and activity measurement results are shown in Table 3-1 and Graph 3-1.
Reaction 01-1 and activity measurement results are shown in Table 3-2 and Graph 3-2.
Table 3-3 and Graph 3-3 show the results of measuring the residual activity when made into an aqueous solution, respectively. From the same data, Example 4 and Control 81 commercially available bath additives were tested in order of activity in a wide temperature range or in a wide temperature range. It was confirmed that each showed a constant high relative activity in the o l-1 region, and also showed a high residual activity with respect to elapsed time in the same order.

Therefore, by comparing commercially available bath additives and Con 1 Herol B, we found that pancreatin, an enzyme obtained from animal organs,
Or, it has been proven that the combination of pancreatin and herbal medicine ingredients exhibits a constant high relative activity over a wide thirst range and a wide pf range, as well as a constant high residual activity over time. It was done.

In addition, a comparison between Control B and Example 4 showed that, compared to pancreatin alone, the product containing ΔSP protease had a constant high relative activity over a wider temperature range, 1) and 1). In addition, it was proven that the residual activity was constant and high over time.

iii) Starch decomposition test This is a comparison of commercially available bath additives containing enzymes that are administered to the organs of animals, Control B, and Example 4. The activity measurement method is to use potato starch as a substrate and to decompose starch through the action of amylase. Straight chain component (amylose)
The amylase activity was determined by measuring the iodine coloration associated with the lowering of the molecular weight.

The reaction temperature and activity measurement results are shown in Table 4-1 and Graph 4-1.
Table 4-2 and graph 4-2 show reaction p-I and activity measurement results.
Table 4-3 and Graph 4-3 show the results of residual activity measurement when dipping into an aqueous solution, respectively. From the same data, Example 4 and Control 81 commercially available bath additives were found to have a wide temperature range in that order. It was confirmed that they exhibited a constant high relative activity over a wide pH range, and a high residual activity over time in the same order.

Therefore, by comparing commercially available bath additives and Control B,
The formulation of pancreatin or a combination of pancreatin and herbal medicine ingredients has a wide temperature range, wide PL, and J
3 shows a constant high relative activity, and 11;'
It was proved that the residual 'fA bamboo remained constant with respect to i changes. Also, the comparison between Control B and Example 4 showed that ASP was more effective in this than in pancreatin alone.
The one with protease added has a wider temperature range, pto1
It has been proven that it shows a constant high relative activity in the range, and also shows a constant high activity over time.
(1) Judgment was made by comparing the residual activities of the degrading activities of proteins, fats, and starches against the elapsed enzyme stabilization time.

i) Residual activity of proteolytic activity Control D and Examples 1 to 4 are compared, and the method for measuring the activity is the same as in the protein degradation test described above. The remaining activity 11 when the storage temperature is 40°C is shown in Table 5-1 and graph 5-1, and the remaining activity 1 when the storage humidity is room temperature is shown in Table 5-2 and graph 5-2, respectively. From the same data, it was confirmed that even in the storage reservoir 1 group with a deviation of 0, each Example showed high residual activity against Con 1-0-C without herbal drug components.

Therefore, it has been proven that the herbal medicine components are responsible for stabilizing the residual activity of proteolytic activity, that is, stabilizing ASP proase.

11) Residual oil t1 of lipolytic effect Control C and Examples 1 to 4 are compared, and the activity measurement method (well, the same as the lipolytic test described above).

Table 6-1 and graph 6-1 show the residual activity when the storage humidity is 40°C, and Table 6-2 shows the residual activity when the storage humidity is room temperature.
, are shown in graph 6-2, and based on the same data, each example is
It was confirmed that it showed a high residual activity of 1% against Roll C. Therefore, the herbal medicine ingredients still have a lipolytic effect = fl
It has been proven that the stabilization of OrI-kuncreazan is helpful.

iii) The residual activity of lυ-degrading action] is compared between Control C and Examples 1 to 4,
The method for measuring activity (it is the same as the lv-particle decomposition test).

The residual activity when storage fA a is 40°C is shown in Table 7-1 and graph 7-1, and the residual activity when storage temperature is room temperature is shown in Table 7.
・-2, Graph 7-2 shows that each example has a high residual activity ft compared to control C at any storage temperature. Therefore, it was confirmed that the crude drug components contributed to stabilizing the residual activity of starch decomposition, that is, to stabilizing pancreatin.

c) Dye stabilization control C1 control D1 control 1-Role E and Example 2 were compared, and the residual rate of dye J
The experiment was carried out using yellow 4-color and blue 1, which is said to be the most susceptible to discoloration among the pigments, and was stored under harsh conditions of 40℃. The m'l determination method was to measure the absorbance of each at the maximum absorption wavelength of 408 μ for Yellow No. 4 and 630 μ for Blue No. 1 to determine the dye residual rate.

Table 8-1 and Graph 8-1 show the measurement results of the dye residual rate of the blue 1-year-old relative to the elapsed time, and Table 8-2 and Graph 8-2 show the measurement results of the dye residual rate of the yellow 1-year-old. From the same data, the dye residual rate of Blue No. 1 with respect to the elapsed time is shown in Example 2, Controls 1 to ○-1, Control D.
1 Con 1 Herol C shows the highest dye residual rate, and Yellow 4F2 also shows the highest dye residual rate in the same order.

Therefore, by comparison with Con 1-Role D and Con 1-Role C, dipentaerythritol fatty acid ester is dye stable+! 1, and a comparison between Con1 Roll D and ] Control E shows that herbal ingredients contribute to dye stability, and a comparison of ] Control Rolls C, D, and E with Example 2 shows that It was proven that even better dye stabilization was achieved when both pentaerythritol fatty acid ester and crude drug ingredients were blended.

2) Heat retention Two types of commercially available bath additives, namely enzyme-containing bath additive A1, herbal medicine-containing bath additive and Example 4, were compared, and 30 healthy people were tested before bathing and at 40 ± 1. After taking a bath in warm water at 0°C, the skin surface temperature of a predetermined part of the body was measured, the average value was calculated, and the temperature change was compared.

Measurement points were the tip of the thumb, the tip of the index finger, the tip of the finger, the back of the finger, and the earlobe. Test tubercles are Table 9-(a), 9
-(h), 9-(c), 9-(d), and 9-(c).

Based on this data, two types of commercially available bath additives have a temperature of 0.00 to 12.0 after bathing.
Compared to the value returned to before the bath at 0 minutes, Example 4 of the present invention
The highest value was reached 10 to 20 minutes after bathing, and even after 120 minutes after bathing, the heat retention was 1.3℃ between the index fingers and 1.3℃ for the earlobe. There is a difference of 4℃, and the temperature will soon return to normal.
It has become clear that 1c0 is present in 1c0. Therefore, it has been proven that a high level of preservation I can be achieved by combining ASP protease, vancreatin, crude drug ingredients, etc. The present invention is not limited to the above examples, and the blending amounts are A(8)\protease at 4'-10%, vancreatin at IfAlθ%, and sodium hydrogen carbonate at Zρ-7°C.
%, IB sodium is ρ, 3~/S%, crude drug ingredients are 4
A-/ρ%, diventaerythritol 1-fatty Ilh acid ester may be about Z^3%.

The present invention can maintain uniform and excellent proteolytic power, fat decomposition power, and starch decomposition power in a wide temperature range or in a wide temperature range, and can have a uniform and excellent proteolytic power, lipolytic power, and starch decomposition power in a wide temperature range, and can be used to improve water quality. Regardless of the difference in water, it can provide excellent cleaning power when used for skin cleansing and washing leftover hot water from a bath. It has the effect of providing a bath additive with excellent stability and heat retention effect.

” ”−'# :zz! ＋＃□□1jER；1llK
Aia [[M Nigelaf 2-1 Reaction temperature and activity measurement results for proteolysis
5N) □Example 4 f'572-8 Oh Mac 2,6. 2. Ukaka□
□. Iyuyo□, -.

7”-1 Lipolysis reaction ffi degree and activity measurement results Table 8-2 Lipolysis reaction PTT and activity measurement results Relative activity (%
) 1---Commercial bath additive m-Control B □Example 4 % Table 8-8 Watery, I! 1ゎOn6B'81 masterpiece □. Workability 8.1 Fruit test Uil [: 20 g/l Temperature: 40℃
1"' Near, 0 Table 4-1 Reaction temperature and activity measurement results for starch decomposition action Relative activity (
%) 1---Commercial bath additive m-Conotrol B Temperature (℃) Relative activity (%) Gu574-2 Reaction P■ of starch decomposition action and activity measurement results Song---Commercial bath agent 11 Conodrol B □Example 4 Table 4-8 Measurement results of residual activity of starch decomposition in aqueous solution Sample concentration: 20 gll m degree: 40'CP■:? ,0
Residual activity (96) Graph 4-8 Table 5-1 Residual activity rate measurement results of proteolytic action Storage temperature 40
°C Storage temperature Room temperature Residual activity rate of proteolytic action Measurement results Graph 5-1 Graph 5-2 1 °-' Residual activity rate of nri lipolytic action. Constant result storage temperature Residual activity rate measurement result of room temperature lipolysis action Graph 6-1 Graph 6-2 Table 7-1 Residual activity rate of starch decomposition action Constant result storage temperature 40℃ Storage temperature Room temperature measurement of residual activity rate of starch decomposition action Result graph 7-1 Graph 7-2 Table S- Blue No. 1 Storage temperature 40℃ Yellow No. 4 Storage temperature 40℃ Color mark residual rate measurement ゛Result table Graph 8-1 1

Claims (1)

[Claims] 1) Proteolytic enzymes originating from microorganisms, enzymes obtained from animal organs such as pancreatin that have proteolytic, lipolytic, and starch decomposing effects, and carrot powder, peony powder, ゛] uboku powder, jinbi powder, etc. A method for producing an enzyme-containing bath additive, which comprises mixing a crude drug extract such as a crude drug powder or A-ubaku extract, adding a pigment thereto via a pigment dissolving agent, and mixing a fragrance to produce a bath additive.