JPS5997058A - Chemical manipulator - Google Patents

Abstract

PURPOSE:To perform the injection of a reagent into a reaction container or the drying thereof without manual operation, by providing a nozzle lifting and falling apparatus, a thermostatic tank and a mixer lifting and falling apparatus with respect to a centrifugal separation apparatus in an up-and-down movable manner. CONSTITUTION:In injecting a reagent in a reaction container 3, a nozzle lifting and falling apparatus 10 is fallen to insert the supply pipe 30 mounted to a reagent emitting nozzle 7 into the container 3. In the next step, when a thermostatic tank and a mixer lifting and falling apparatus 17 are raised toward a centrifugal separation apparatus 1, a taper body 3 is contacted with the container 3 to incline the same. By this mechanism, the pipe 30 is contacted with the inner wall of the container 3 and about mul of the reagent held by the leading end of the pipe 30 is supplied to the container 30. In drying the container 3, a drying suction nozzle 8 is closely contacted with the container 3 to perform drying. By this method, the injection of the reagent into the reaction container and the drying of the same can be performed without manual operation.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a chemical manipulator, and in particular to a novel improvement for automating analytical processes such as mixing microliter samples and cold ridge heating.

The microliter-order analysis process used in the past was extremely complex, and most of it was done manually by researchers.

The object of the present invention is to provide extremely effective means for quickly eliminating the above-mentioned drawbacks, and in particular, it is an object of the present invention to provide a centrifugal separator equipped with a rotary table that is rotatably provided and has a reaction vessel; It has a nozzle up and down device that has a reagent discharge nozzle and a suction nozzle for drying and can be moved up and down, and a constant temperature bath and mixer up and down device that has a constant temperature bath and a mixer that can be moved up and down. This is a configuration in which the nozzle up/down device, the constant temperature bath, and the mixer up/down device are moved up and down relative to the separation device.

Hereinafter, a preferred embodiment of the chemical manipulator according to the present invention will be described in detail with reference to the drawings.

In the drawings, reference numeral 1 indicates a centrifugal separator, and a rotary table 4 having a plurality of reaction vessels 3 at its circumferential end is rotatably mounted on a drive shaft 2 of the centrifugal separator 1. A detection disk 5 is provided approximately at the center of the drive shaft 2, and one end of this detection disk 5 is arranged so as to overlap with a rotational positioning sensor 6 whose entire shape is U-shaped. The centrifugal separator 1 is capable of detecting the rotational position of the table 4, and the reaction vessel 3 is rotatably provided in the direction of the arrow A as shown by solid lines and dotted lines in FIG. A nozzle up/down device 10 having a holding plate 9 for holding the reagent discharge nozzle, the nozzle 7 and the drying suction nozzle 8 is disposed at the upper position. A shaft 11 is slidably provided on the holding cylinder 12, and the shaft 1
1 moves up and down in the direction of arrow A.

Furthermore, a constant temperature bath 14 is located at the bottom of the centrifugal separator 1.
and a mixer 15 at its peripheral end.
A constant temperature bath and a mixer up/down device 17 are provided,
A support shaft 18 integrally provided with this holding plate 16 is provided in a holding cylinder 20 having an electromagnetic plunger 19 so as to be movable up and down in the direction of arrow B, and is held by operating the electromagnetic plunger 19. Plate 16 is arrow B
It is configured to be able to move up and down in the direction of. In addition, drive shaft 2
, axes 11 and 18 are located on the same center line.

The reagent sent from the reagent liquid supply device 21 is discharged to the reagent discharge nozzle 7 on the order of μt, and the drying suction nozzle 8 is connected to a condenser 23 connected to a cooling device 22. The output of is connected to a vacuum pump 24. The constant temperature bath 14 is connected to a temperature control device 25 and controlled to a constant temperature, and the output control signal from the sequence controller 26 is connected to the vacuum pump 24, the rotational drive motor 1α of the centrifugal separator 1, the constant temperature cypress, and the mixer. It is supplied to the electromagnetic plunger 19 of the up/down device 17, the reagent liquid feeding device 21, and the i-magnetic plunger 13 of the nozzle up/down device 10, and controls each of them.

Further, a longitudinal reagent supply pipe body 30 is attached to the reagent discharge nozzle 7, and a tapered body 31 is provided at the peripheral end of the holding plate 16. The tapered body 51 has a tapered portion 32 on one side as shown in FIG.
When the state shown in B in the figure is reached, the tapered portion 32 is attached to the reaction vessel 3.
The reaction vessel 6 is inclined along the tapered portion 32 in order to join the outer peripheral portion of the reaction vessel 6 . In this state, the holding plate 9 is lowered at the same time, so the tip of the reagent supply pipe 30 joins the inner 1111 part 3a of the reaction vessel 3, and the reagent supply vibrator 30
Normally, the reagent in units of several μt supplied from the reagent supply vibro 0 becomes spherical due to interfacial tension at the tip of the reagent supply vibro 0 and does not fall.
a, flows down the inner part 3α and is guided to the bottom part.

Also, at this time, although the pipe 30Fi needs to be bent, the flexible tube 50b section has flexibility, and the coil spring 30α has a structure that has the function of restoring the flexible tube 3ob to its straight state.

In the above configuration, when the chemical manipulator according to the present invention is operated, the electromagnetic plunger 13 operates to lower the holding plate 9, and the reagent sent from the reagent feeding device 21 is transferred to the reagent discharge nozzle 7. The reagent is injected into the reaction container 3 in μ units with the supply tube 30 in contact with the inner side 3α of the reaction container 3.Furthermore, reagents are injected into other reaction containers 3 provided on the rotary table 4. In this case, the rotary drive motor 1α is rotated by a predetermined angle using control signals from the sequence controller 26, and
The other reaction container 3 is rotated to a position corresponding to the reagent discharge nozzle 7.

When the electromagnetic plunger 13 is operated again in this state, the holding plate 9 is lowered, and a unit of μ of the reagent is injected from the reagent discharge nozzle 7 into the reaction container 3 by the same operation as described above.

Furthermore, when removing moisture in the reaction container 3, the rotary drive motor 1α is operated to rotate the rotary cheatle 4, the reaction container 3 is positioned at a position corresponding to the drying suction nozzle 8, and the electromagnetic plunger 13 is moved. When operating ζ, the retaining plate 9
drops and the drying suction nozzle 8 comes into close contact with the reaction container 3,
The liquid in the reaction vessel 3 after centrifugation is removed by a condenser 23 and sucked up by a vacuum pump 24.

In addition, when the temperature of the sample in the reaction container 6 is to be rapidly raised or lowered, the electromagnetic plunger 19 is operated to raise the holding plate 16, which causes the thermostatic chamber 14 to rise and the reaction container 6 to be placed inside the thermostatic chamber 14. The temperature inside the reaction vessel 3 is changed to the temperature set in the constant temperature bath 14 within a certain period of time.

Therefore, according to this embodiment, operations such as centrifugation of the sample in the reaction container 3, reagent injection, drying, temperature change, and mixing can be performed extremely easily, accurately, and automatically by the sequence controller without any manual intervention. The effect of this is that it is now possible to quickly carry out difficult work that has traditionally been done by many researchers, which is extremely beneficial. In particular, the structure of the reagent supply pipe is flexible and resilient, and the fact that the pipe can always be positioned on the center line of the reaction vessel is extremely important for reliably supplying reagents. It is something that brings about an effect.

[Brief explanation of the drawing]

FIG. 1 is an overall configuration diagram showing a chemical manipulator according to the present invention, and FIG. 2 (A) ω) (C) is a configuration diagram showing the main parts. 1...Centrifugal separator 3...Reaction container 3α...Inner part 4...Rotary table 6...Rotation position sensor 7...Reagent discharge nozzle 8...Drying suction nozzle 9...
Holding plate 10... Nozzle up/down device 14... Constant temperature bath 15... Mixer 16... Holding plate 17... Constant temperature bath and mixer up/down device 21... Reagent liquid feeding device 22... Cooling device 23... Condenser 24... Vacuum pump 25...Temperature control device 26...Sequence controller 30...Reagent supply pipe body 30α...Spring coil 3i...Flexible tube 31...Taper body 32...Tenoku part. Applicant Daini Seikodai Co., Ltd. Agent Patent Attorney Mogami Figure 2 (A) Figure 2 (C> Figure 2 (B)

Claims (1)

[Claims] A centrifugal separator having a rotary table that has a plurality of reaction vessels and is rotatably provided; a rotation position detection section for detecting the rotational position of the rotary table; A nozzle holder having a constant temperature bath and a mixer, a constant temperature bath and a mixer vertically movable device movable in the direction of its spindle, and a reagent discharge nozzle and a drying suction nozzle provided at an upper position of the centrifugal separator. a sequence controller for controlling the operations of the reagent discharge nozzle, the drying suction nozzle, the centrifugal separator, the constant temperature bath, and the mixer up/down device; and a bendable longitudinal pipe body provided in the reagent discharge nozzle. and a tapered body provided on the constant temperature bath and the mixer vertical device, and when the constant temperature bath and the mixer vertical device are raised, the tapered body tilts the reaction vessel,
A chemical manipulator characterized in that the pipe body is bent and joined to an inner wall of a reaction vessel so that a reagent from the pipe body is supplied into the reaction vessel.