JPH0422611B2 - - Google Patents
Info
- Publication number
- JPH0422611B2 JPH0422611B2 JP62179911A JP17991187A JPH0422611B2 JP H0422611 B2 JPH0422611 B2 JP H0422611B2 JP 62179911 A JP62179911 A JP 62179911A JP 17991187 A JP17991187 A JP 17991187A JP H0422611 B2 JPH0422611 B2 JP H0422611B2
- Authority
- JP
- Japan
- Prior art keywords
- suction
- processing container
- stirring
- discharged
- reagent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000012545 processing Methods 0.000 claims description 30
- 239000007788 liquid Substances 0.000 claims description 24
- 239000003153 chemical reaction reagent Substances 0.000 claims description 22
- 238000003756 stirring Methods 0.000 claims description 22
- 238000000034 method Methods 0.000 claims description 16
- 238000002156 mixing Methods 0.000 claims description 7
- 210000004369 blood Anatomy 0.000 description 6
- 239000008280 blood Substances 0.000 description 6
- 239000000243 solution Substances 0.000 description 5
- 238000004140 cleaning Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 2
- 238000010876 biochemical test Methods 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000003028 elevating effect Effects 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- 230000001965 increasing effect Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 208000007536 Thrombosis Diseases 0.000 description 1
- 238000004159 blood analysis Methods 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012805 post-processing Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明は血液等の分析装置における試料と試薬
の混合撹拌等、複数等の混合撹拌方法に関するも
のである。DETAILED DESCRIPTION OF THE INVENTION (Industrial Application Field) The present invention relates to a method for mixing and stirring a plurality of methods, such as mixing and stirring a sample and a reagent in a blood analyzer.
（従来の技術）
一般に血液等の生化学検査に際しては、サンプ
ル溶剤の遠心分離処理、上澄もしくは沈降部分の
分取処理、分取部分への試薬混合処理、吸光度そ
の他の測定等の各処理を順次行うこものであり、
例えば外科手術に先立つ血液の分析においては採
取した全血を一定時間静置した後、遠心分離機に
かけ、血清と血餅とを分離させ、その上澄の血清
のみを別の処理容器に一定量づつ分注し、これに
希釈液を加えて希釈し、この各処理容器毎に所望
の反応液を加えて震盪し、反応後測定機にかけて
吸光度等の測定を行うようにしている。(Prior art) In general, when performing biochemical tests on blood, etc., various processes such as centrifugation of the sample solvent, preparative processing of the supernatant or precipitated portion, mixing of reagents to the preparative portion, and measurement of absorbance and other matters are carried out. It is a series of activities,
For example, in blood analysis prior to surgery, collected whole blood is allowed to stand for a certain period of time, then centrifuged to separate serum and blood clots, and only the supernatant serum is transferred to a separate processing container in a fixed amount. A diluting solution is added to dilute the solution, a desired reaction solution is added to each processing container, the solution is shaken, and after the reaction, the absorbance is measured using a measuring device.
このような分析に際し、従来は試薬注入後の撹
拌については、第３図に示すような撹拌装置
A′で行つていた。これはモーター等の駆動源に
より回転する撹拌棒ａを処理容器M′に挿入して
行うものである。 Conventionally, for such analysis, stirring after reagent injection was performed using a stirring device as shown in Figure 3.
I was going with A′. This is done by inserting a stirring rod a rotated by a drive source such as a motor into the processing container M'.
この他、液体の撹拌には、容器内に収容した複
数種類の混合液内に空気吐出ノズルを挿入し、空
気の噴出により撹拌させるようにしたもの（実開
昭61−37225号公報）がある。 In addition, for stirring liquids, there is a method in which an air discharge nozzle is inserted into a mixed liquid of multiple types stored in a container, and the mixture is stirred by blowing out air (Japanese Utility Model Publication No. 37225/1983). .
（発明が解決しようとする課題）
しかしながら以上のような従来の撹拌は、試薬
を処理容器へ注入後に別の機械的な、又は流体的
な運動源を利用した撹拌装置により別工程で行つ
ていたので作業装置及び作業工程が多くなるとい
う問題があつた。(Problem to be Solved by the Invention) However, in the conventional stirring as described above, after the reagent is injected into the processing container, it is performed in a separate process using a stirring device using another mechanical or fluid motion source. Therefore, there was a problem that the number of working devices and working processes increased.
本発明は以上のような従来の問題に鑑みてなさ
れたものであり、その目的は、試料と試薬等の複
数液の混合撹拌を容易迅速に行なうとともに、装
置の低廉化を図ることのできる複数液の混合撹拌
方法を提供することである。 The present invention has been made in view of the above-mentioned conventional problems, and its purpose is to easily and quickly mix and stir multiple liquids such as samples and reagents, and to reduce the cost of equipment. An object of the present invention is to provide a method for mixing and stirring liquids.
（課題を解決するための手段）
上述の如き従来の問題を解決し、初期の目的を
達成するための本発明の特徴は、計量ポンプに連
結され、先端を吸排ノズルとした一本の吸排管内
にその先端の吸排ノズルより空気を吸引させた
後、混合しようとする複数液を順次吸引させ、然
る後、該吸排ノズル先端を処理容器内に挿入して
該処理容器の底面近傍まで接近させた後、前記吸
排管内の複数液及び空気を吸排ノズルより吐出さ
せることにより、該吸排ノズルの先端が先に吐出
された液中に沈んだ状態で吐出される空気によ
り、これら複数液を撹拌することにある。(Means for Solving the Problems) A feature of the present invention for solving the above-mentioned conventional problems and achieving the initial objective is that a single suction/discharge pipe connected to a metering pump and having a suction/discharge nozzle at its tip. After suctioning air through the suction/discharge nozzle at the tip, multiple liquids to be mixed are suctioned one after another, and then the tip of the suction/discharge nozzle is inserted into the processing container and brought close to the bottom of the processing container. After that, the plurality of liquids and air in the suction/discharge pipe are discharged from the suction/discharge nozzle, and the plurality of liquids are agitated by the air discharged with the tip of the suction/discharge nozzle submerged in the previously discharged liquid. There is a particular thing.
（作用）
而して上記構成によれば、吸排管内に吸引され
た空気、及び混合しようとする液は、両液及び空
気の順に処理容器内に吐出され、一方の液がその
吐出の際に前に吐出された液と予備混合されると
ともに、両液が全て吸排管内から吐出されてその
先端がこれらの液中に挿入されている状態で空気
が続いて吐出されるので、該空気が気泡となつて
さらに両液を撹拌して混合させる。(Function) According to the above configuration, the air sucked into the suction/discharge pipe and the liquid to be mixed are discharged into the processing container in this order, and one of the liquids is In addition to being premixed with the previously discharged liquid, air is subsequently discharged with both liquids being discharged from the suction and exhaust pipes and their tips inserted into these liquids, so that the air forms bubbles. Then, both liquids are further stirred to mix.
（実施例）
以下、本発明の一実施例を図面に基づいて説明
する。(Example) Hereinafter, an example of the present invention will be described based on the drawings.
第１図は本発明の方法で撹拌する分析装置Ａの
概略図を示したものであり、ターンテーブル１
と、先端を吸排ノズルとした吸排管２と計量ポン
プ３及び洗浄ポンプ４とにより構成される。 FIG. 1 shows a schematic diagram of an analyzer A that stirs according to the method of the present invention.
It is composed of a suction/discharge pipe 2 whose tip is a suction/discharge nozzle, a metering pump 3, and a cleaning pump 4.
ターンテーブル１は軸５を中心にモーター６を
もつて高速回転自在に支持され、かつ、軸５は角
度検出用の円板（図示せず）が固定され、この円
板によりモーター６の駆動を制御して必要な角度
の回転、停止がなされるようにしている。 The turntable 1 is supported with a motor 6 around a shaft 5 so as to be able to rotate at high speed, and a disc (not shown) for angle detection is fixed to the shaft 5, and the motor 6 is driven by this disc. It is controlled so that it rotates and stops at the required angle.
また、ターンテーブル１の周縁部には一定間隔
毎に処理容器支持部７が設けられ、該処理容器支
持部７には各種の試薬ｍが入つた処理容器Ｍが嵌
入されている。 Further, processing container support portions 7 are provided at regular intervals on the periphery of the turntable 1, and processing containers M containing various reagents m are fitted into the processing container support portions 7.
一方ターンテーブル１の上方には処理容器支持
部７のいずれかの位置の停止位置上方に対応して
体検移し変え用の吸排管２が昇降装置２ａによつ
て上下動自在に支持されている。 On the other hand, above the turntable 1, a suction/discharge pipe 2 for transferring the physical examination is supported so as to be vertically movable by an elevating device 2a, corresponding to a stop position above any position of the processing container support 7. .
この吸排管２には、流路８を介して計量ポンプ
３及び洗浄ポンプ４が直列に連結され、その末端
が洗浄液タンクＴに連通されて該洗浄液ｔを切換
えバルブ９を介して吸排管２に供給する。 A metering pump 3 and a cleaning pump 4 are connected in series to this suction/discharge pipe 2 via a flow path 8, and the end thereof is communicated with a cleaning liquid tank T, and the cleaning liquid t is transferred to the suction/discharge pipe 2 via a switching valve 9. supply
本装置Ａは、このように配置された各部材を順
次動作させて処理を行うものであるが、その動作
はマイクロコンピユーターを組み込んだ自動制御
機構をもつてなされる。 This apparatus A performs processing by sequentially operating each member arranged in this way, and the operation is performed by an automatic control mechanism incorporating a microcomputer.
次に本発明の試薬ｍの撹拌方法をこの装置Ａを
利用した血液の生化学検査に基づいて説明する。 Next, a method of stirring the reagent m of the present invention will be explained based on a blood biochemical test using this apparatus A.
処理に先立ち採取した全血を処理容器Ｍに収容
し、ターンテーブル１の所定の処理容器支持部７
に設置する。 The whole blood collected prior to processing is stored in a processing container M, and the whole blood is placed in a predetermined processing container support portion 7 of the turntable 1.
to be installed.
また、次の処理容器支持部７とは分析に必要な
数の別の処理容器M1を設置し、これらを設置し
た後自動制御機構を作動させ、予め設定されたプ
ログラムに沿つて自動的に遠心分離処理、上澄も
しくは沈降部分の分取処理、分取部分への試薬混
合処理等が行われる。 In addition, separate processing vessels M 1 of the number necessary for analysis are installed in place of the next processing vessel support 7, and after these are installed, the automatic control mechanism is activated to automatically control the process according to a preset program. Centrifugation treatment, fractionation treatment of the supernatant or precipitated portion, and treatment of mixing reagents into the fractionation portion are performed.
前記分析工程における混合処理の場合は、第２
図中ａに示すように計量ポンプ３の動作により吸
排管２に適量の空気ｓを吸引する。この状態で第
２ｂ図に示すように吸排管２を昇降装置２ａによ
り降下させて試薬ｍの入つた処理容器Ｍ内に挿入
して試薬ｍを吸引する。 In the case of the mixing process in the analysis step, the second
As shown in a in the figure, an appropriate amount of air s is sucked into the suction and exhaust pipe 2 by the operation of the metering pump 3. In this state, as shown in FIG. 2b, the suction/discharge tube 2 is lowered by the elevating device 2a and inserted into the processing container M containing the reagent m, and the reagent m is aspirated.
次いで、吸排管２を上昇させるとともに、ター
ンテーブル１を旋回させて該吸排管２に対応する
位置に試料ｎの入つた処理容器M1を位置させる。 Next, the suction/exhaust pipe 2 is raised, and the turntable 1 is rotated to position the processing container M1 containing the sample n at a position corresponding to the suction/exhaust pipe 2.
そして、第２図ｃに示すように該吸排管２を再
び昇降装置により下降させて試料ｎの入つた処理
容器Ｍ内に挿入し、計量ポンプ３の動作により該
試料ｎを吸引して上昇させる。 Then, as shown in FIG. 2c, the suction and exhaust pipe 2 is lowered again by the lifting device and inserted into the processing container M containing the sample n, and the sample n is sucked and raised by the operation of the metering pump 3. .
而して、吸排管２には空気ｓ、試薬ｍ、試料ｎ
の順にこれらが吸引される。 Therefore, air s, reagent m, and sample n are supplied to the suction and exhaust pipe 2.
These are suctioned in this order.
次に、ターンテーブル１の処理容器支持部７に
支持された空の処理容器Ｍが吸排管２に対応する
位置に位置するようにターンテーブル１を回転さ
せるとともに、第２図ｄに示すようにこれら試薬
ｍ、及び試料ｎが吸引された吸排管２を昇降装置
２ａにより下降させて処理容器Ｍ内に挿入させ、
その先端の吸排ノズルを底面近傍まで接近させ
る。 Next, the turntable 1 is rotated so that the empty processing container M supported by the processing container support part 7 of the turntable 1 is located at a position corresponding to the suction and exhaust pipe 2, and the turntable 1 is rotated as shown in FIG. The suction/discharge tube 2 into which the reagent m and the sample n have been sucked is lowered by the lifting device 2a and inserted into the processing container M,
Bring the suction/discharge nozzle at the tip close to the bottom surface.
そして、この状態で第２図ｅ，ｆに示すように
計量ポンプ３の作動によりこれら試料ｎ、試薬
ｍ、空気ｓを吐出させる。 In this state, the sample n, reagent m, and air s are discharged by operating the metering pump 3 as shown in FIG. 2e and f.
この際、試料ｎが処理容器Ｍに吐出されると吸
排管２の先端ノズルがこの試料ｎ内に沈んだ状態
となるため、次の試薬ｍが吐出されるときには試
料ｎと試薬ｍとが予備混合さる。そして第２図ｇ
に示すように試薬ｍが全て吐出された後、空気ｓ
が続いて吐出される。該空気ｓは試料ｎと試薬ｍ
内に吐出されて小さな気泡s1となつてこれらを瞬
時に撹拌混合する。 At this time, when the sample n is discharged into the processing container M, the tip nozzle of the suction/discharge tube 2 is submerged in the sample n, so that when the next reagent m is discharged, the sample n and the reagent m are in reserve. Mixed. And Figure 2g
After all the reagent m has been discharged as shown in
is then ejected. The air s contains the sample n and the reagent m.
They are discharged into the air and become small bubbles, which are instantly stirred and mixed.
また、この撹拌方法は、前述のような分析装置
Ａを使用せずとも、その他の装置及び手法による
撹拌も可能である。 In addition, this stirring method does not require the use of the above-mentioned analyzer A, and can also be performed using other devices and methods.
（発明の効果）
本発明は以上のような方法にしたことにより下
記の効果を有する。(Effects of the Invention) By employing the method described above, the present invention has the following effects.
本発明は混合しようとする複数液を同一容器
に注入すると同時に撹拌されるため、従来のよ
うに注入工程の後撹拌装置を挿入するという工
程及びその撹拌装置を省くことができるので、
混合液の撹拌を迅速容易に行うことができ、無
人の自動化が容易である。 In the present invention, since multiple liquids to be mixed are poured into the same container and stirred at the same time, the conventional step of inserting a stirring device after the injection step and the stirring device can be omitted.
Stirring of the mixed liquid can be done quickly and easily, and unmanned automation is easy.
混合しようとする液を吐出後、それらの溶液
中に空気を吐出することにより瞬時にこれらを
撹拌するので撹拌効果を高めることができる。 After discharging the liquids to be mixed, air is discharged into the solutions to instantaneously stir them, thereby increasing the stirring effect.
従来は処理容器の前で試料と試薬等の複数液
を合わせた後処理容器に注入して反応作業をし
ていたが、本発明は、反応作業をこれらを処理
容器内で瞬時に行うことができる。 Conventionally, reaction work was performed by combining multiple liquids such as a sample and reagent in front of a processing container and injecting them into a post-processing container, but the present invention allows the reaction work to be performed instantly within the processing container. can.
第１図は分析装置の該略図、第２図は本発明の
試料と試薬の撹拌方法を示す断面図、第３図は従
来の試薬の撹拌方法を示す断面図である。
２……吸排管、３……計量ポンプ、ｍ……試
薬、ｎ……試料、ｓ……空気、４……処理容器。
FIG. 1 is a schematic diagram of an analyzer, FIG. 2 is a sectional view showing a method of stirring a sample and reagent of the present invention, and FIG. 3 is a sectional view showing a conventional method of stirring a reagent. 2... Suction and exhaust pipe, 3... Metering pump, m... Reagent, n... Sample, s... Air, 4... Processing container.
Claims (1)
した一本の吸排管内にその先端の吸排ノズルより
室気を吸引させた後、混合しようとする複数液を
順次吸引させ、然る後、該吸排ノズル先端を処理
容器内に挿入して該処理容器の底面近傍まで接近
させた後、前記吸排管内の複数液及び空気を吸排
ノズルより吐出させることにより、該吸排ノズル
の先端が先に吐出された液中に沈んだ状態で吐出
される空気により、これら複数液を撹拌すること
を特徴とする試料と試薬の混合撹拌方法。1. Room air is sucked into a single suction/drainage tube connected to a metering pump and has a suction/drainage nozzle at its tip, and then multiple liquids to be mixed are suctioned one after another. After inserting the nozzle tip into the processing container and bringing it close to the bottom of the processing container, the plurality of liquids and air in the suction/discharge pipe are discharged from the suction/discharge nozzle, so that the tip of the suction/discharge nozzle is discharged first. A method for mixing and stirring a sample and a reagent, which is characterized by stirring the plurality of liquids using air discharged while submerged in the liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62179911A JPS6427626A (en) | 1987-07-21 | 1987-07-21 | Method for agitating reagent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62179911A JPS6427626A (en) | 1987-07-21 | 1987-07-21 | Method for agitating reagent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6427626A JPS6427626A (en) | 1989-01-30 |
| JPH0422611B2 true JPH0422611B2 (en) | 1992-04-20 |
Family
ID=16074070
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62179911A Granted JPS6427626A (en) | 1987-07-21 | 1987-07-21 | Method for agitating reagent |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6427626A (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0710226B2 (en) * | 1990-06-20 | 1995-02-08 | 株式会社ピーシーシーテクノロジー | Protoplast stirring device, cell fusion device and flow cytometer device including the same |
| US7144447B2 (en) * | 2001-06-19 | 2006-12-05 | Voest-Alpine Industrieanlagenbau Gmbh & Co. | Method and device for treating particulate material |
| JP5284928B2 (en) * | 2009-11-20 | 2013-09-11 | 株式会社日立ハイテクノロジーズ | Liquid mixing method and dispensing device |
| JP6610127B2 (en) * | 2015-09-28 | 2019-11-27 | 株式会社島津製作所 | Liquid dispensing apparatus and liquid dispensing method |
| WO2020037671A1 (en) * | 2018-08-24 | 2020-02-27 | 深圳迈瑞生物医疗电子股份有限公司 | Blood sample analyzer, blood sample analysis method, and computer storage medium |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6137225B2 (en) * | 1976-08-05 | 1986-08-22 | Fuiritsupusu Furuuiranpenfuaburiken Nv |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6137225U (en) * | 1984-08-09 | 1986-03-07 | 住友電気工業株式会社 | Stirring device |
-
1987
- 1987-07-21 JP JP62179911A patent/JPS6427626A/en active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6137225B2 (en) * | 1976-08-05 | 1986-08-22 | Fuiritsupusu Furuuiranpenfuaburiken Nv |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6427626A (en) | 1989-01-30 |
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