JPS5977360A - Liquid feeder for analysis - Google Patents

Abstract

PURPOSE:To feed more than one kinds of reagents with only one liquid feeding pump by branching the discharge side of a feeding pump into two or more of liquid passages so that one reagent flows through one branch path while the other branch path is provided with a reagent filling vessel for filling other kind of reagent. CONSTITUTION:A valve 19-1 is opened and a reagent filling vessel 14 is filled with a B reagent through a reagent supply path 15. After the end of the filling, the valve 19-1 is closed. Then, a valve 18-1 is opened while a valve 18-2 closed, an A reagent is fed to a mixing chamber 13. Then, the valve 18-2 is opened while the valve 18-1 closed, the B reagent in the reagent filling vessel 14 is extruded with the A reagent to be fed to the mixing chamber 13.

Description

[Detailed Description of the Invention] This invention is based on the t word FIA (Flow In
jec - mouth on Analysis) etc. 2
This invention relates to an analytical liquid feeding device that feeds, mixes, and sends more than one cup of reagent to a reaction coil or the like.

1i' When it is necessary to flow reagents of Class 1 or higher in two or more channels in IA, etc., two or more liquid pumps (lli (flow rate stability, lifespan, Alternatively, this can be done using a small plunger pump (preferably a small plunger pump), since the flow rate can be set independently, but since multiple pumps are used, the reliability of the device is somewhat lacking.On the other hand, If a squeeze-type pump with two or more channels is used, only one liquid transfer pump is required, but the flow rate cannot be changed independently, and there are problems with flow stability and lifespan.Therefore, this invention was developed. We have tried to resolve the above problems and improve the reliability of the equipment.

Our goal is to provide an analytical liquid delivery device that is satisfactory in terms of flow rate stability, etc.

The configuration and embodiments of the present invention will be described below with reference to FIGS. 1 to 3.

The analytical liquid feeding device according to the present invention includes a liquid feeding pump (11)
, a liquid flow path 112) for separately feeding reagents of class 2i'ilj and above, and a mixing chamber for mixing two or more types of iic', , j, and qi by merging these liquid flow paths t121. (
In an analysis liquid feeding device comprising a liquid feeding pump (111 as one unit).

The suction side is connected in a flow path to a reagent container (not shown) containing one sample; the discharge side is connected to two or more liquid flow paths (12
-1), +12-2), . . . , and among them, the l branch 1i',') (12-1) is made to flow the l reagent, and the other branch flow path (12- 2),...
. . . are each provided with a reagent filling container +ta for filling a separate tank f (this reagent filling container f1
Reagent supply "i," which supplies the reagent after 411 is completed;
51 is provided separately, and the three branch channels (, 1, 2-1)
, I l 2-2), ...... confluence (16
Each control f before 1! ηl device (valve 118-1 whose opening/closing is controlled by 171), I 18-
2), . . . are provided to alternately feed the sampled hollyhocks into the mixing chamber (13).

The first drawing is an embodiment of the present invention, and is a schematic diagram showing the flow path arrangement of the FIA. Reagent A (hereinafter referred to as A reagent).

Note that this reagent may simply be a carrier liquid that carries the sample. )) is sent from the suction side of the liquid pump (11j). - A universal type of reagent (hereinafter referred to as B reagent) is supplied through the reagent supply channel (19-1+) by manually opening the valve (19-1+)
15) into the reagent filling container αa. In the figure +
19-2) are valves for draining, and these valves (19-1) and (19-2) are closed after filling with the B reagent. And branch flow path +12-1
), (12-2) In front of the confluence fl [il O) (7) Ha (electro-a valve) (18-1), (18-2
)j, t.

These are controlled by a controller (171) to open and close alternately. That is, when the valve (18-1) is open, the valve +18-2 is closed, and the A reagent is added to the mixing chamber (1).
Send it to 31. Next, when the valve (18-2) is open, close the valve (18-1) and reagent filling container (
Push out the B reagent filled in 141 with the A reagent.

Send the B reagent to 10,000 in the mixing chamber (13). Figure 2 shows the situation. Here, the set flow rate per unit time of the liquid feed pump 01) is Q, the opening time of the valve (18-1) (the closing time of the valve (18-2)) in one cycle of valve opening and closing is TA, and the valve I 18 -2) When opening IIl
'J (closing time of valve (18-1)) is TB, and the flow rates of reagent A and reagent B per unit time are 0 person and Qn, respectively.

TA TFI """TA+TB Q'7QB=TA+TB Q・
Q=QA+QIl. In this way, the valve (18-1
), +18-2) depending on the opening/closing time of reagent A and B.
The reagent will be sent to the mixing chamber (131 million).The time for one cycle of opening and closing the valve should be as short as possible in order to sufficiently mix the A reagent and B reagent. It is desirable that the internal dimensions be as small as possible so that reagent A and reagent B are difficult to mix.

Since the part where reagent A and reagent B are mixed in the reagent filling container +141 cannot be subjected to zero analysis, that part is
In step 1, refill the reagent container with reagent B and use it for the next analysis. In the figure, ■ is the sample injector, and this position is the branch flow path +12-1), (12-2
) may be on the front side of the merging point (1G) or on the rear side of the 0 merging point (16). C! 1) is the reaction coil, +2
``a'' is a detector. t5 Figure 3 shows another embodiment in which three types of reagents are passed through 9 reaction coils f21+
FIG. 2 shows a flow path diagram of the analytical liquid delivery device in the case where there are two trees.

By configuring the analytical liquid feeding device as described above, two or more types of reagents can be fed with one liquid feeding pump, thereby increasing the reliability of the device.

In addition, by appropriately setting the set flow rate of the liquid pump and the opening/closing time of the valve, the flow rate of each sample patch can be changed, and problems in terms of flow rate stability and service life do not occur.

[Brief explanation of drawings]

FIG. 1 is a flow path configuration diagram of a liquid delivery device for analysis according to an embodiment of the present invention, and FIG. 2 is a diagram illustrating a state in which two types of reagents are delivered by the device. FIG. 3 is a diagram showing another embodiment of the invention. Further, FIG. 4 is a flow path configuration diagram of a conventional device. ii...Liquid sending pump 12...Liquid flow path 13...Mixing chamber 14...Reagent filling container 15...Reagent supply path ■ 6... Merging point 17... Controller 18-1.18-2...
····valve! -De3shij Publication Agent Patent Attorney Kitamura 7 Old J-・Fig. 1 17 Fig. 2 Fig. 3 Fig. 4

Claims (1)

[Claims] An analytical liquid transfer device comprising a liquid transfer pump, a liquid flow path for separately feeding two or more types of reagents, and a mixing chamber for merging these liquid flow paths to mix 217 or more reagents. In this method, there is one pre-delivery liquid pump, the suction side thereof is connected in a flow path to a reagent container containing l reagents, and the discharge side is branched into two or more liquid flow paths, of which l liquid flow path The above-mentioned reagent 1 is allowed to flow through the channel, and each of the other branch channels is provided with a reagent filling container filled with a different type of reagent,
A reagent supply channel for sequentially supplying the reagent to this reagent filling container is provided separately, and a reagent supply channel is provided before the confluence of the branch channels.
1. A liquid feeding device for analysis, characterized in that a valve is provided which is controlled to open and close by a controller so that various reagents are alternately fed into a mixing chamber.