JPS5963182A - Cultivation of basidiomycetes, and increase of raising - Google Patents

Abstract

PURPOSE:In cultivating and raising Basidiomycetes, to aim increase in mycelia or fruit body of Basidiomycetes, cultivating and raising it in the presence of cinnamic acid (or related substance). CONSTITUTION:In cultivating and raising Basidiomycetes such as Lentinus edodes, Pholiota nameko, Flammulina velutipes, Armillaria matsudake, etc. by wood cultivation, bottle cultivation, box cultivation, etc., cinnamic acid or a substance related to it is dissolved in ethanol or hot water to give about 1-0.0001 wt% solution, and added to a culture solution to be used. When wood for cultivating Basidiomycetes is used, the solution is sprinkled upon the surface of the wood sufficiently, or the wood is immersed in the solution, and the Basidiomycetes is cultivated. Consequently, the yield of fruit body is increased by about 60-80wt%, and the yield of mycelia is 1.9 times as much as that of an existing method. Coumaric acid, caffenic acid, ferulic acid, sinapic acid, etc. are preferably used as the substance related to cinnamic acid.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for cultivating basidiomycetes and increasing yield in cultivation.

More specifically, the present invention relates to a method for culturing or cultivating basidiomycetes in the presence of cinnamic acid or its related substances to increase the yield of basidiomycetes.

In general, basidiomycetes produce bacterial aggregates by culturing, or
Like shiitake mushrooms, they are cultivated by cultivating the fruiting bodies using wood, or by cultivating the fruiting bodies in containers. However, one of the drawbacks is that the growth of basidiomycetes is slow and the production efficiency is low, regardless of whether it is cultivated or cultivated.

As a result of research to improve the poor production efficiency of Basidiomycetes, the present inventors found that by culturing or cultivating them in the presence of cinnamic acid or related substances, We were able to increase our actual revenue.

The present invention is a method for culturing and cultivating basidiomycetes to increase yield, which is characterized in that basidiomycetes are cultured and cultivated in the presence of cinnamic acid or a substance related thereto.

In the present invention, when culturing or cultivating basidiomycetes,
Cinnamic acid or related substances are present.

Specific examples of the basidiomycetes include shiitake, nameko, enokitake, oyster mushroom, furitake, mukitake, wood ear, slime mushroom, moegitake, shirodamogitake, myko, tsukuritake, matsutake, versicolor, stonecrop, coffisarnokoshika, tsugasarnokoshika, and the like.
In the present invention, these are effectively produced.

Cinnamic acid and related substances used in the present invention include cinnamic acid, fumaric acid ('trans-hydroxycinnamic acid),
Caffeic acid (3',4-dihydroxycinnamic acid), ferulic acid (4oxy-6methoxycinnamic acid), 5-hydroxyferulic acid, sinapic acid (6,5-dimethoxy4-hydroxycinnamic acid) and their contents Things can be given.

Cinnamic acid or its related substances are dissolved in ethanol or hot water to be present at a concentration of about 0.1 to 0.000% when culturing or cultivating basidiomycetes.

Specifically, cinnamic acid and cinnamic acid-related compounds are added to liquid culture, aeration culture, shaking culture, etc. of these basidiomycetes in wood cultivation, 9-box cultivation in bottles, synthetic media, semi-synthetic media, etc. It is.

When using Hoda wood, spray it sufficiently on the surface of the Hoda wood, or soak the Hoda wood in an additive solution. If an artificial medium such as a sawdust medium is still used, cinnamic acid, etc. may be added from the beginning, or a solution added with cinnamic acid, etc. may be poured into the container at the start of cultivation.

Furthermore, in culturing, cinnamic acid or the like is added to the culture solution in advance depending on the type of bacteria used.

Cultivation of the fruiting bodies is carried out in a conventional manner depending on each basidiomycete. This culture is expected to increase the yield of fruiting bodies by 60 to 80%.

Furthermore, in culturing basidiomycetes, a culture solution may be inoculated with basidiomycetes and cultured according to each basidiomycete. Through this culture, the yield of bacterial conglomerates can be increased approximately 1.9 times.

Next, examples of the present invention will be shown.

Example 1 Two-year-old Quercus alba trees were divided into seven groups, and 1 was placed in an aquarium filled with water as a ferulic acid-free group (control), and 2 was placed in an aqueous tank containing 0005% aqueous solution as a ferulic acid-added group. It was added to a water tank containing an aqueous solution of ferulic acid dissolved in 90% ethanol. Both No. 1 and No. 2 were immersed in water at a temperature of 17° C. for 6 hours and then taken out, and each Hoda tree was allowed to sprout and develop at the same location. The fruiting bodies were harvested on the 5th and 6th day after soaking, and the fresh weight (g) and dry weight (g') were determined. The results are shown in the following table.

Two-year-old Quercus alba trees were divided into seven groups, one group was treated with no ferulic acid (control), and two were treated with 90% ethanol.
Into a water tank containing 601 mL of 0.00 ml of water, 2 is a ferulic acid addition group containing 200 mL of 90% ethanol in which ferulic acid has been dissolved to make an aqueous solution with a concentration of 0.005%.
It was added to a water tank containing 607 ml of an aqueous solution containing snl. It was treated and harvested in the same manner as in Example 1. The results are shown in the following table.

Example 6 Peptone 6g Molybdic acid 3m9 Glucosfila Og Zinc chloride 3m9 Phosphoric acid - SO 0,
5y Manganese chloride 3■%f Magnesium acid 0
．． 5I copper sulfate 1 my calcium chloride o
, ig distilled water 10100O ferrous sulfate 0.01. l
i' phi 4.5 Thiamine 0.01g Above peptone glucose medium 50. +n71 each 3
After sterilization, add caffeic acid (0.001% concentration in the medium) and ferulic acid (0.001% concentration in the medium) to a 0.00 ml Erlenmeyer flask.
005% concentration), sinapinic acid (0005% concentration in the medium)
was dissolved in 0.5 mA' of ethanol and then added to the medium. In addition, a medium to which only 0.5 m/ml of ethanol was added was prepared for comparison. Prepare 10 bottles for each medium,
Shiitake fungus was inoculated. The culture was statically cultured at 25°C for 60 days. After culturing 5 of the 10 plants, wash them with water and store them at 60°C.
After drying for 24 hours, it was weighed. The remaining five plants were examined for fruiting body development. The results are shown in the table below.

Agent Patent Attorney 1) Parent Male

Claims (1)

[Claims] A method for cultivating and cultivating basidiomycetes and increasing yield, which comprises culturing basidiomycetes in the presence of cinnamic acid or a substance related thereto.