JPS5932087B2 - Pearl growth promotion method - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention is a method for growing pearls, which is characterized by treating the pieces with an aqueous solution containing three types of mixed aqueous solution containing inosinic acid or ribonucleic acid before cell division, protein enzymes, and enlarging beads. Pertains to the Promotion Act.

The inventor has already patented a method for promoting animal growth using pre-cell division colchicine and ribonucleic acid (441031, 8-838,
9, 11,), but as a result of repeated trials for about 15 years, a triple combination method in which inosinic acid in ribonucleic acid or its decomposition product was added to a protein enzyme and colchicine was used to produce the nucleus of Akoya shellfish. It has been found that during insertion surgery, the piece process is highly effective in increasing the thickness of the mother-of-pearl layer.

Pearl farming involves inserting a small, perfectly round nucleus artificially made from a freshwater shell, and causing a pathological phenomenon within the body of the Akoya oyster. It is made from a type of protein called conchiolin, which accounts for about 5% of the secondary ingredients, and water to create pearls, natural gemstones with shine and thickness.

A piece is the mantle of the Akoya shellfish, and is first cut into a 2-3 mm wide strip around the so-called peace color line, and then cut into a rectangular shape by cutting it into 2-3 mm lengths.

The piece is inserted into the mother shell while holding the nucleus.

There are already many activators on the market to improve the functionality of this piece, but there is still no one that is highly effective.

The piece grows with its epithelium and connective tissue to enclose the nucleus, and then becomes a nacreous sac, thickening the nacre around the nucleus.

Therefore, in this case, rather than strengthening the Akoya shellfish as a whole, the division promotion of the peace cells themselves, cell expansion,
The aim is to increase the amount of secretions.

The increase in biochemical reactions in animals and plants, especially the ability to synthesize practical proteins, is a goal that is difficult to achieve. It has been found that the above-mentioned piece cells themselves are useful for promoting division.

Protein enzymes include papain, pepsin, chymotrypsin,
Tryfusin, cathepsin, chymosin, thrombin...
There are various types, but any of them will be effective.

In the case of pepsin, it is effective at pH 2-5, but a pH adjuster may not be used, and no bactericide or the like should be used.

The same applies to other enzymes.

Inosinic acid has long been discovered as a component of meat extracts, and is now a type of nucleotide produced by the deamination of adenylic acid obtained from ribonucleic acid extracted from yeast. Its molecular formula is Cl0H13N403P, and it is currently used as a seasoning. The sodium 5-ribonucleotide used is its N
This was used because it is a salt and is more water-soluble.

Ribonucleic acid was prepared from yeast, and Na salt was used in consideration of its water solubility.

Before cell division, there are various drugs such as colchicine, chlorohydrate, zulphon drugs, antibiotics, etc., but colchicine was considered to be appropriate in previous tests, so this was used.

The reason for limiting the concentration of each component is patent, 4296611゜Atsushi 4
4102.4410318... etc.
Since a value centered around I ppm of 100 ppm or less is appropriate, this experiment was also carried out in the same manner as in the examples.

Example Akoya shellfish that were made for three years were used, six sections of 100 pieces each including an untreated section were prepared, and pieces were cut from the mother shells for the pieces.

There are two insertion nuclei per page, and the size is 6.7 in diameter.
The average values were 0.4.1 mm and 0.4.1 mm.

After the surgery, the shellfish were transferred to a vertical cage and suspended temporarily for one month, after which they were finally hung offshore.

The piece processing was completed by simply dropping each aqueous solution onto a rectangular piece on a wooden board and allowing it to seep out naturally for a few minutes.Then, the nucleus insertion surgery was immediately performed as usual, and the piece was taken to Sakaide.

The surgery was performed on April 5th, and on January 9th of the following year, 10 shells were opened at random from among the remaining pages, excluding the shellfish that had been previously destroyed.
I took out the beads and got the results shown in the table below.

Out of 1.00 spinach shellfish, the highest number was 25 in the untreated area, and the number gradually decreased to a minimum of 15 in the test area.

(1) Exam number An aqueous liquid with the following composition was tested.

The concentration of each component was 1 ppm.

Flame beads are those that are perfectly round and can be sold with curls, luster, and shine, while scrap beads are beads that are not perfectly round, have mottled color, are not a single color, or are whitish and dull. , which cannot be sold because it has a blackish-brown color.

(3) Regarding the size of beads and practical methods only) (unit: in)
As can be seen from the table above, 41-3 did not have much effect compared to the untreated group, but the mixed use of //64.5 sufficiently met the requirements of the present invention.

Also, in the flat size 4.5, there were 3 8Tt71L balls that were not in the untreated area.
There were 2 pieces.

As shown in the above test, the application method other than peace treatment lasts for at least 9 months with just a few drops and soaking for a few minutes. Possible treatment methods include leaving it in the air for a while and then returning it to seawater to promote the growth of the mother mollusk, or dropping an appropriate amount into the mother mollusk during surgery to increase the number of beads along with the mother mollusk.

As a formulation method, sodium inosinate (sodium ribonucleic acid)
0.0IP, pepsin, colchicine each 0.01
Mix the three types of P, homogenize with 100 cc of distilled water to create a suspended aqueous solution, pasteurize and store, and when using, add 10 cc of the stock solution to 1 oocc of distilled water and shake well to obtain a concentration of 1 ppm. It is used as a treated water solution.

Claims (1)

[Claims] 1. Before cell division, with a piece treated with a three-part mixture of protein enzymes and inosinic acid or ribonucleic acid,
A pearl growth promotion method for enlarging beads, which is characterized by inserting the nucleus into the mother oyster in a state in which it is exposed.