JPS5919857A - Reaction method of trace amount of sample - Google Patents

Abstract

PURPOSE:To simplify operation and to perform efficiently the operation by suspending and supporting a reactor freely oscillatably in a radial direction to a turntable which is freely rotatable at a high speed, feeding a sample and a reagent therein, and rotating the turntable at a high speed thereby mixing the sample and reagent to cause reaction. CONSTITUTION:A sample and a reagent are incorporated in respective containing vessels 14, 15, and an empty reactor 1 is set in a turntable 2. A sample dropping nozzle 11 is first lowered and about one drop... several drops of the samples are dropped along the wall surface of the reactor 1. The dropping is accomplished by turning successively the turntable 2 by each required times when the other kind of reaction is required. Thereafter, a reagent dropping nozzle 12 is lowered to drop the reagent along the wall surface of the reactor 1. When the turntable 2 is rotated at a high speed after the dropping and feeding of the sample and the reagent, the reactor 1 oscillates in the direction where the bottom end is swung outward and the sample and the reagent are moved toward the bottom of the reactor 1 by centrifugal force, whereby mixing is accomplished.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for reacting a micro-open sample, such as for blood type determination, in which a reagent is mixed with a small amount of sample and reacted.

Conventionally, flat glass plates with shallow recesses for reactions have been used when conducting reaction tests using minute amounts of samples, such as blood type determination, or when conducting multiple various tests using minute amounts of samples. A υ nozzle and a reagent are placed on it, and the mixture is mixed and reacted within the recess. However, all of these conventional methods are performed manually and require a lot of time and effort for handling notebooks, injecting and mixing reagents, and are therefore inefficient.

In view of the above-mentioned problems, the present invention aims to provide a reaction method that can automatically perform the reaction of a trace amount of sample without requiring human intervention. A 4) A small-diameter bottomed cylindrical container is suspended from the periphery of a given turntable so as to be swingable in the radial direction of the turntable. This method consists of injecting a sample and a reagent into the container, and then collecting the injected sample and reagent at the bottom of a container by rotating a turntable at high speed, and mixing and stirring the sample and reagent.

Next, embodiments of the present invention will be described with reference to the drawings.

When carrying out the method of the present invention, as an example, the method shown in the figure (
Use position. This device has a turntable 2 that supports a reaction vessel 1 around its periphery.

This turntable 2 is rotatably driven by a motor 3, and its angle is detected by a disc 4 for displaying the rotational angle position and a sensor 5, so that it can be stopped at a desired position. , and can be rotated at high speed by motor 3.

On the periphery of the turntable 2, reaction vessel support parts 6, 6, . . . are provided at regular intervals. This support part 6
As shown in FIGS. 2 and 3, a pair of support bins 7. are provided in a concave portion formed by recessing the circumferential edge of the turntable 2 in the radial direction, and are opposed to each other and protrude in the direction of rotation of the turntable 2. A container support ring 8 is rotatably supported by 7.

A test tube-shaped reaction container 1 is suspended and supported by this container support ring 8.

On the other hand, above the turntable 2 is a vertical drive means 9a.
, 9b, the support substrates 10a, 1 are moved up and down separately.
0b, a sample dropping nozzle 11 is provided on the supporting substrate 10a, and a plurality of reagent dropping nozzles 12 are provided on the supporting substrate 10b, each corresponding to a different reaction vessel stopping position. Both nozzles 11 and 12 are communicated with 4) a nozzle storage container 14 and a reagent storage container 15 via constant molar pumps 13 and 13, respectively. Furthermore, each nozzle 11.
'+2 is oriented in a different direction so that it does not touch the same position of the reaction vessel 1, thereby preventing the nozzle from being contaminated by the liquid dropped earlier.

J3, when the tips of both nozzles 11 and 12 are bent toward the inner wall surface of the reaction vessel 1 and lowered into the reaction vessel 1, the tips of both nozzles 11 and 12 are bent toward the inner wall surface of the reaction vessel 1. It is placed so that it is in contact with the wall.

When carrying out a reaction using such an apparatus, the sample and reagent are placed in their respective storage containers 14 and 15, the empty reaction container 1 is set in the turn table 2, and the sample dropping nozzle 11 is first lowered and the sample dripping nozzle 11 is lowered. , a sample in an amount of one to several drops is dropped onto the wall of the reaction vessel 1. If other types of reactions are required, this dropping is performed by sequentially rotating the turntable 2 as many times as necessary.

After that, the reagent dropping nozzle 12 is lowered to the reaction vessel 1.
Drop the reagent along the wall of the container. Note that several types of drugs may be separately stored in each reagent storage container 15, and the turntable 2 may be rotated to drop them at the required positions.

Furthermore, if only a small amount of sample is present, it may be manually injected into the reaction container 1 and set on the turntable 2.

After dropping the sample and reagent in this way, the turntable 2 is rotated at high speed. As a result, the reaction container 1 is swung in the direction in which the lower end is shaken out, and the sample and reagent are moved to the bottom of the reaction container 1 by centrifugal force and mixed.

Incidentally, the dropping of samples and reagents by the pump and the rotation of the turntable as described in (above) above are performed automatically and sequentially by an automatic control unit incorporating a microcomputer according to a preset program. Furthermore, it goes without saying that the reaction container may be suspended and supported by a container support ring that is always supported at an angle with respect to the turntable.

The reaction method of the microboiler nozzle of the present invention is configured as shown in (a) above, in which a reaction container is suspended and supported by a turntable that can rotate at high speed so as to be swingable in the radial direction, and the sample and reagent are injected into the inside of the reaction container. By rotating the turntable at high speed to mix and react samples and reagents, the reaction of minute samples, which was conventionally carried out on a glass plate and had to be done manually, can now be turned automatically. This method can be carried out on a table, and it greatly simplifies and improves efficiency, especially when a large number of different trace samples are reacted with the same reagent, such as in a blood test for a group of people. This is what was to be done.

[Brief explanation of the drawing]

The drawings show an example of the apparatus used in the method of the present invention; Fig. 1 is a sectional view showing the overall outline, Fig. 2 is a plan view of the turntable, and Fig. 3 is an enlarged view showing the state of dropping into the reaction container. FIG. DESCRIPTION OF SYMBOLS 1...Reaction container, 2...Turntable, 6...Reaction container support part, 11...Sample dropping nozzle, 12...
・Reagent dripping nozzle, 13...pump.

Claims (1)

[Claims] 1. A cylindrical, narrow-diameter container with a bottom is suspended and supported on the periphery of a turntable capable of providing high-speed rotational drive so as to be swingable in the radial direction of the turntable, and a nozzle is inserted along the inner surface of the container. A reaction method for a trace sample C, which is characterized by injecting a sample and reagent in a finer volume, and then rotating a turntable at high speed to collect the injected sample and reagent at the bottom of the container and stirring them.