FR2530819A1 - METHOD FOR REACTING A SAMPLE CONTAINING A TRACE STATUS SUBSTANCE - Google Patents

Abstract

THE INVENTION RELATES TO CHEMICAL ANALYSIS. TO REACT A SAMPLE CONTAINING A SUBSTANCE IN THE TRACE STATE, THIS SAMPLE AND A REAGENT IS INTRODUCED IN REACTION 1 CONTAINERS WHICH ARE TILTING SUSPENDED FROM THE PERIPHERY OF A TURNTABLE 2, AND THE LAST TURNTABLE 2 IS TURNED. AT HIGH SPEED SO THAT THE SAMPLE AND THE REAGENT ARE CENTRIFUGAL AND GATHER AT THE BOTTOM OF CONTAINERS WHERE THEY MIX. ALL OPERATIONS, INCLUDING THE INTRODUCTION OF THE SAMPLE AND THE REAGENT THROUGH INPUT TUBES 11, 12, ARE PERFORMED AUTOMATICALLY, ACCORDING TO A PREDETERMINED PROGRAM. APPLICATION TO THE DETERMINATION OF THE BLOOD GROUP.

Description

The present invention relates to a method for reacting a sample

  containing a trace substance, by mixing this sample with a reagent to produce a reaction, for example for the purpose of determining the blood group. A flat glass slide equipped with one or more low reaction cavities is usually used.

  depth, to perform the reaction test of a sample

  Trace containing a trace substance, such as the blood group test or various tests using a sample containing a trace substance. The sample and reagent are placed on the slide and mixed in. the cavity for producing the reaction The method of the prior art of this type must be implemented entirely manually, and requires a great deal of

  work and time to manipulate the blade and to introduce

  mix and mix reagent The process of the prior art

therefore has poor productivity.

  To eliminate the disadvantages of

  the prior art described above, the object of the invention is to provide

  a reaction process that does not require manual operation to react the sample containing a

  trace substance, this process making it possible to

  the reaction automatically

  essential elements of the invention are that

  Reaction trays in the form of small diameter cylinders with a bottom are suspendedly supported by the peripheral portion of a turntable capable of rotating at a high speed in such a way that they can freely rock into the cylinder. radial direction of the turntable; a reagent and a sample containing a trace substance are then placed in the containers on their inside surface; and then we turn

  the turntable at high speed so that the

  and the reagent introduced into the

  collect at the bottom of the containers and mix and

to produce the reaction.

  The invention will be better understood on reading the

  description that follows of an embodiment and in

  referring to the accompanying drawings which represent an example of

  the apparatus used to implement the process of the in-

and on which:

  Figure 1 is a schematic sectional view of

the entire apparatus;

  Figure 2 is a plan view of the turntable

  ing; and Fig. 3 is an enlarged section showing the state in which a reagent is added to the reaction vessel. In order to implement the invention, an apparatus such as that shown in the drawings is used as an example. This apparatus comprises a rotating plate 2 which supports containers in its circumferential part.

  of reaction 1 The turntable 2 is driven in rotation

  by a motor 3, and a disc 4 and a sensor 5 detects

  the rotation angle to indicate the angle of rotation and the position The turntable can be stopped at a necessary position and the motor 3 can turn it to

a great speed.

  Reaction vessel holders 6, 6 are disposed in the circumferential portion of the tray

  rotating 2 with predetermined intervals between them.

  As shown in FIGS. 2 and 3, each reaction vessel support 6 comprises a pair of support journals 7, 7 which project face to face in the direction of rotation of the turntable 2, within cavities

  formed in the circumferential parts of the turntable

  2, in the radial direction of the latter, for the purpose of rotatably supporting a support ring of

3 'container 8.

  The reaction vessel 1, in the form of a test tube, is suspended from this support ring of

container 8 and supported by it.

  Support discs 1a, 1bb, which are raised and lowered independently by means of drive in the vertical direction, 9a, 9b, are arranged above the turntable 2 A drop-in introduction tube drop

  of sample 11 and several drop-in introduction tubes

  reagent drop 12 are respectively arranged on the

  support disc 1 Oa and the support disc lob, so

  corresponding to the separate stop positions of

  The two introduction tubes communicate with a sample reservoir 14 and with a reagent reservoir 15, via

  respective dosage 13, 13 Each of these introducer tubes

  11, 12 is placed in such a way that its direction is different from that of the others, to prevent the tube comes into contact with the same position of the reaction vessel 1 and is contaminated by the liquid that has been

  added previously drip.

  The free end of each insertion tube 11, 12 is curved towards the surface of the inner wall of the reaction vessel 1, so that when the introduction tube is lowered and introduced into the reaction vessel 1, its end come in contact with the wall of

reaction vessel 1.

  The reaction is carried out using the apparatus described above, in the following manner.

  place the sample and the reagent in the reservoirs

  14, 15, and empty reaction vessels 1 are placed in the turntable 2. The sample drop-in tube 11 is first lowered, so that

  add drop by drop one or more drops of sample

  lon, along the wall of the reaction vessel 1 When several kinds of reactions are necessary, this addition is repeated by successively rotating the turntable.

  The tasting tube is then lowered.

  reagent drop 12, so as to add the reagent dropwise along the wall of the reaction vessel 1. Several kinds of reagents can be stored in

  separate reagent reservoirs so as to be added

  drop by rotating the turntable

  2 to bring it to the necessary positions When the

  the trellis is in a trace state, it can be introduced manually

  in the reaction vessel 1, which can then be

in the turntable 2.

  Once the sample and the reagents have been added dropwise as described above, the turntable 2 is rotated at high speed, so that the reaction vessel 1 tilts in a direction such that its bottom end is moved outward, and the sample and reagent contained in the reaction vessel are moved to the bottom of

  the latter and are mixed by centrifugal force.

  The addition of the sample and the reagent by the pumps and rotation of the turntable are done sequentially by an automatic control mechanism which includes a microcomputer, operating in accordance with a predetermined program -It is obviously possible to use a structure in which the reaction vessel is suspendedly supported; by container support rings that are always supported in an orientation

  oblique to the turntable.

  The process according to the invention for reacting

  a sample containing a trace substance

  characteristics described above Containers

  reaction are suspended, 9 by the

  rotating drum capable of rotating at a high speed, with a configuration such that the reaction vessels can tilt in the radial direction, and the sample and

  reagents are placed in the respective reaction vessels.

  The method of the invention thus makes it possible to automatically perform the reaction of a sample containing a substance in a trace state which is placed on the turntable, whereas this reaction was not previously carried out. In particular, when one has to react with the same reagent many different kinds of samples containing a substance in the trace state, as in the case of the hematoscopy of a group of subjects, the method of the invention greatly simplifies the work

  exam and greatly improves productivity.

  It goes without saying that many modifications can be made to the process described and shown,

  without departing from the scope of the invention.

Claims (1)

CLAIM   A process for reacting a sample containing a substance in a trace state, characterized in that   reaction vessels having the shape of a cylinder of   The diameter with a bottom is sus- pended, suspended, by the peripheral part of a turntable which can be rotated at a high speed, in such a way that the containers can tilt freely in the radial direction of the plate. rotating, a sample containing a trace substance and a reagent   the reaction vessels along their integral surface   then rotate the turntable at high speed so that the sample and reagent   in the containers gather at the bottom of these containers.   and mix and stir.