JPS59104321A - Preparation of medicinal substance from leaf of sasa albo-marginata - Google Patents

Abstract

PURPOSE:To extract the medicinal substance completely from the leaves of Sasa albo-marginata, by drying and cutting the raw leaves of the vegetable into fine pieces, immersing in water, boiling the water to concentrate the water-soluble substances, adding another concentrated liquid prepared in the similar manner as above, boiling the mixture at a specific temperature, separating the supernatant liquid from the product, and boiling and concentrating the liquid. CONSTITUTION:Raw leaves of Sasa albo-marginata are dried and cut to fine pieces. The cut leaves are immersed in water in a pan to effect the dissolution of the water- soluble substances, and the solution is concentrated to a proper extent by boiling to obtain the first concentrated liquid. Another concentrated liquid is prepared in a similar manner as the above process, and is mixed with a small amount of the first concentrated liquid. The mixture is heated for 6-12hr until the temperature reaches 120- 160 deg.C, mixed with warm water, and boiled. The supernatant liquid is separated from the product, concentrated by boiling, and cooled to precipitate the objective medicinal component. The precipitate has a molecular weight of >=10,000, and 30% of which is determined mainly as the polysaccharide of formula (galacturonic acid). The medicinal component containing said saccharide can be extracted more completely by this process.

Description

DETAILED DESCRIPTION OF THE INVENTION This invention is based on the patent application Sho 6 O 6 O Specification (Japanese Patent Application Kokai Sho 6 O 6 O), which is characterized in that a medicinal substance can be industrially produced in large quantities from leaves at the eye level without using special solvents. 5o-itoai
Publication No. v relates to an improvement in the method of producing an eye-effect substance from Yakuma bamboo leaves, (1). A step of drying fresh leaves at eye level and shearing them finely as a source material, placing them in a pot and submerging them in water to elute water-soluble substances, boiling them, and concentrating them as appropriate to create a -C second concentrated solution; Fresh leaves at eye level are dried, finely sheared, placed in a pot, soaked in water, water-soluble substances are eluted, boiled, concentrated as appropriate, and an appropriate amount of the first concentrated solution is added and mixed. Make a liquid and make the mixture /20C, //, 01
::Continue heating for 4 to 12 hours until it reaches the desired temperature, add warm water to it, boil it, remove the supernatant liquid, boil and concentrate, and then cool and precipitate it. or (2) to obtain the final Bi3 effect substance fC. Dry the fresh leaves at eye level.
71, use the finely sheared material as the raw material, put it in a pot and soak it in water, boil it to exude the water-soluble substances, and add η- as appropriate.
Cover the pot with a lid while it is still in the stiff state, apply a high pressure of about 7 atm, and bring it to a boiling temperature of /6θj? f
A process of heating at a temperature of 2 degrees for about 6 to 2 hours to extract the medicinal solution to create a first concentrated solution, drying the fresh leaves at the eye level, finely shearing them, putting them in a pot and submerging them in water, Water-soluble substances are eluted and boiled down to concentrate as appropriate.
A suitable amount of the first concentrated solution is added to make a mixed solution, and the mixture is boiled down until the temperature reaches 20 U/60 U, and heating is continued for 2 hours. Add warm water to this, boil it, remove the supernatant liquid, boil it and concentrate, then
According to the present invention, the viscosity of the extraction solution increases and the solution is heated to high temperature. Therefore, the concentration was effective/), and it was possible to extract and concentrate the medicinal substance a from the leaves at the eye position without using any special solvent. ), the bad side effects caused by solvents are completely eliminated, and by performing heating extraction under high pressure, it is possible to increase the extraction temperature of the liquid at the eye level and heat it. It is extremely useful as it can perform complete extraction work and concentrate.

It is known that the ocular extract has diuretic, anti-inflammatory, and anti-tumor effects.

Conventionally, the method for extracting medicinal substances from this type of medicinal herb is to boil the medicinal herb, which has been dried for many years, in water in an earthenware pot. Water is heated over a low heat for a long time, avoiding strong flames.

For example, multiply 6ooca/2 hours to half the amount, 300.
It is boiled down to 0.00 and the supernatant liquid is divided into doses and taken every day.

According to this conventional method, since the heat treatment is performed under normal pressure, even if a high-pressure pot is used, the boiling temperature is approximately ii.
oc, i, 2o U or more cannot be expected.

Therefore, it is completely impossible to extract the medicinal polysaccharide at the eye level, which is extracted only at about /lOU or more.

Focusing on this point, in order to extract medicinal substances from leaves at the eye position without using special solvents, the prior patent application No. 1II
In the invention disclosed in No. 1, the raw material is dried and finely sheared fresh leaves at eye level, which is placed in a pot and soaked in water to exude water-soluble substances, boiled, concentrated as appropriate, and left as it is. Then, cover the pot, apply high pressure of about 7 atm, raise the boiling temperature to about /60 t:', and heat for about 6-72 hours to extract the medicinal substances. It has been developed to produce medicinal substances from the leaves.

But this advance! Since the concentration of the extracted medicinal substance cannot be said to be perfect with the nuclear method, the invention of this application does not require prior patent application. Pot.

Specification of Japanese Patent Application Publication No. 29 (Japanese Unexamined Patent Application Publication No. 2003-101001) A condensed solution is used as a first concentrated solution, and a small amount of this is dried and smoked with fresh leaves of Kashiwa, and finely sheared. The medicinal polysaccharides are completely extracted by placing the medicament in a pot and submerging it in water to elute the water-soluble substances, boiling it, adding it to the concentrated mixture as appropriate, and then subjecting it to an extraction and collapsing process. With,
That tP t? = It is possible to manufacture medicinal substances by raising the temperature and completely concentrating it.According to the invention of this application, (1), the raw material is dried and finely sheared fresh leaves of Kashi. Put this in a pot and submerge it in water to elute the water-soluble substances, boil it, concentrate it as appropriate, and make the first 4 condensed solution. The water-soluble substances were eluted, boiled, and appropriately concentrated, and an appropriate amount of the first concentrated solution was added to prepare a mixed solution. The final medicinal substance is obtained by continuing heating for several hours, adding warm water to it, boiling it, removing the supernatant liquid, boiling and concentrating it, and then cooling and precipitating it. Alternatively, (2) Dry and finely shear fresh leaves of the 6-kuma bamboo grass as the source material, put it in a pot, soak it in water, exude the water-soluble substances, boil it, concentrate it as appropriate, and leave it as it is. Cover the pot with a lid, apply high pressure of about 7 atm, raise the boiling temperature to about 1601:', and heat for about 6 to 12 hours to extract the medicinal solution and make the first concentrated solution. The process of making a dried and finely sheared fresh leaves of Kashira is put in a pot and soaked in water to elute the water-soluble substances, boiled and reduced to an appropriate size, and then an appropriate amount of the first concentrated solution is added. 7.20 U of the mixed solution.
A step in which the mixture is boiled down to ~1toc and then heated for 12 hours, hot water is added to it, the mixture is boiled, the supernatant is taken, the mixture is concentrated at a boiling point, and then it is cooled and precipitated. Since the final medicinal substance is obtained by sequentially applying
It is extremely useful because it allows the extraction and concentration of << to be carried out more completely and the production of the medicinal substance to be carried out more effectively.

1st Example Dried fresh leaves of Kuseki are finely sheared and used as a raw material, put into a circulation chamber, soaked in about 10 times the volume of water, boiled and concentrated to about 5 liters to form a first concentrated solution. make.

The raw material for this is essentially composed of the cell membranes and intercellular substances of the leaves. Its chemical composition is approximately 3j% carbohydrates, ≠6~gf% protein, approximately 3~1% phospholipid and cholesterol as lipids, and minerals CαNα.
, s7. Approximately 73 to 1 lq6 are present.

Separately, the fresh leaves of Koushi dried in the same manner as above were prepared by Iv (TI7
5. Use the sheared material as raw material and put it into an enamel pot.

When immersed in j times the volume of water, eluting water-soluble substances, boiling and concentrating to 70 liters, approximately 3~j
a quantity of the first concentrated solution is added to form a mixed solution. In that case, the viscosity force l of the mixed liquid increases and the mixed liquid i
, boil it until it reaches 20C-IAOc, continue this for about 4 to 12 hours, add warm water to it, boil it, and boil it. The clear liquid is taken, boiled and concentrated, and then cooled and precipitated to produce the desired medicinal substance.

Therefore, this precipitate has a molecular weight of 70,000 or more, and
0chi is mainly [-+ll) -a-D gal
It has been recognized that FA-(1-'l+tgalph-(l→]n) is a polysaccharide galacuro/acid.

Second Example: The raw material is finely sheared leaves of dried bonito flakes,
Put this in a pot, soak it in about io times the amount of water, boil it, and reduce
Concentrate into. If this is placed in a pot as it is, the pot is covered with a lid, and the inside is heated to 7 atmospheres, reaching /lOC. The /401:: is continuously extracted for 4 to 72 hours to prepare a -0 condensation solution.

Separately, raw leaves of bonito flakes dried in the same manner as above were finely sheared and placed in an enamel pot.
When immersed in twice the amount of water, eluted water-soluble substances and boiled down, and concentrated to 7/70 m, approximately 3~j-
amount of the first gamma layer solution to form a mixed solution. In that case, the viscosity of the mixed liquid increases, making this mixed liquid /,
It means that it can be heated up to 2(1; l C ~ ito U, so it is f /20 c, /601:'
This was continued for about J-12 hours,
Hot water is added to the mixture, the mixture is boiled, the supernatant liquid is removed, and the mixture is boiled and condensed, and then cooled and precipitated to produce the desired medicinal substance.

Relationship with Hiroshi addition This invention relates to an addition invention to the patent application Akihiro patent specification No. O (Japanese Patent Publication No. 57-6 OJ, No. 2u).
The patented invention is based on the process of drying and finely shearing fresh leaves of bonito flakes, placing them in a pot and submerging them in water, exuding water-soluble substances, boiling them, and concentrating them as appropriate. The method is to extract the medicinal solution by heating the pot for about 6 to 1 hours at a high pressure of about 7 atm and increasing the boiling temperature to about 1 toc. However, in this invention, (1). Dry and finely sheared fresh leaves of Katebushi are used as the raw material, which are then placed in a pot and soaked in water to elute water-soluble substances, boiled, and appropriately concentrated to create a first concentrated solution; Dry-smoked and finely sheared (f): Placed in a pot and submerged in water, eluted water-soluble substances, boiled and concentrated as appropriate; add appropriate amounts of the first concentrated solution to make a mixture; Mixed liquid/20
Heating is continued for 6 to 12 hours until reaching C~/lO'c, hot water is added to this, boiled, the supernatant is taken, concentrated by boiling, and then cooled and precipitated. li) to obtain the final medicinal substance. The raw material is dried and finely sheared leaves of Katsubushi, which are placed in a pot and submerged in water to exude water-soluble substances, boiled, concentrated as needed, and placed in the pot with a lid. Apply a high pressure of about 7 atm to bring the boiling temperature to about ito c.
The process involves heating at a moderately high temperature for about 6 to 2 hours to extract the medicinal solution and making a first condensation solution, and then drying the fresh leaves of bonito flakes and cutting them finely into a pot and submerging them in water. , water-soluble substances are eluted, boiled and saturated appropriately,
Adding an appropriate amount of the first concentrated solution to prepare a mixed solution;
Continue to heat the mixture for 1-12 hours until it reaches t/20C~#OCK, add warm water to it, boil it, remove the supernatant liquid, boil and concentrate, and then cool it. and precipitation step to obtain the final medicinal substance.
Compared to the invention (% Komei 37-111) J Koko No. Publication), the extraction and concentration of medicinal substances from the leaves of bonito can be carried out more completely, and the medicinal substances can be produced more effectively.

Inventor Takashi Otani
Yoshi Patent Applicant TOC Co., Ltd.

Claims (2)

[Claims] (1) Dry and finely shear fresh eye leaves as the raw material, put it in a pot and soak it in water to elute the water-soluble substances, boil it, and shrink it appropriately to make the first concentrated solution. process and
Dry and finely sheared fresh leaves of the eye part are placed in a pot and soaked in water to elute water-soluble substances, boiled and concentrated appropriately, and then add an appropriate small amount of the first concentrated solution to make a mixture. , the mixture was heated at 6°C until it reached /20C to /10C.
Continue heating for ~12 hours, add warm water and boil,
A method for producing a medicinal substance from optic leaves, characterized in that the final medicinal substance is obtained by sequentially performing the steps of removing the supernatant liquid, subjecting it to AX boiling concentration, and then cooling and precipitating it. (2) Dry and finely shear the fresh leaves of the eye part as the source material, put it in a pot and soak it in water, boil it to exude the water-soluble substances, concentrate it as appropriate, and leave it as it is. Cover the pot, apply high pressure of about 7 atm, raise the boiling temperature to about /40 C, and heat for about J-/2 hours to extract the chemical solution and perform the first concentration. The process of making a solution,
Dry and finely shear fresh eyes leaves, put in a pot and submerge in water to elute water-soluble substances, boil and boil down to concentrate, add an appropriate small amount of the first concentrated solution, and mix. A liquid was prepared, and the mixed liquid was boiled down until it reached lλoC・~/lOC, and heating was continued for t-72 hours,
The final medicinal substance is obtained by sequentially performing the steps of adding warm water to the mixture, boiling it, removing the supernatant liquid, boiling and concentrating it, and then cooling and precipitating it. A method for producing medicinal substances from the leaves of .