JPS5881044A - Pasturization of liquid separation apparatus - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention is directed to a novel method for sterilizing liquid dispensing devices.

As methods for sterilizing liquid devices that are applied to living organisms, methods using chemical agents such as formalin water and ethylene oxide gas, methods using high-pressure steam, and methods using T@heat irradiation have been proposed. Of these, formalin It has been pointed out that water has residual toxicity and can cause liver cirrhosis, and in gas sterilization using ethylene oxide, the adsorbed plastic is said to be hemolytic. Naota steam sterilization method and gamma ray irradiation sterilization method are attracting attention as methods.

As a result of extensive research into the sterilization method for a new separation device incorporating a porous membrane hollow fiber that communicates micropores with the outer wall surface, an excellent #2 effect was created from the following method. Separation device incorporating a hollow fiber σ) Inside or outside the hollow fiber, add a liquid that can mix with the water bath liquid and that can penetrate into the pores inside the hollow fiber. By replacing the hollow fiber with a liquid and performing r-ray irradiation with the hollow fiber Tt in a wet state,
Particularly preferred are polyethylene hollow fibers having the following properties. As long as it is characterized by its structure and has a porosity of JO-90vo1%, it is an ethylene hollow fiber (1) consisting of microfibrils arranged in the longitudinal direction and knots connected almost at right angles to the microfibrils. The average thickness of the microfibrils (dM) and the average length (7M) of the microfibrils are (LM = QO2~Q3fi 1M; a/~40μ), and ( J) The average length of the ToW6 in one fiber direction (7k) is 1t = (itN40μ), and the average width (dv) and average length IV) of the strip-like micropores are 4 Ma/6M=QJN! There is a relationship of I / 6v = j ~ go (j)
The average pore diameter of the micropores measured with a mercury porosimeter is Q/-1 μ.

A liquid separation device in which a cylindrical container is filled with such hollow fibers is useful for living organisms, especially as a device for extracting plasma from blood among body fluids, or as a device for filtering and separating proteins in plasma. Although it is essential to sterilize the device in order to use it, it is undesirable for devices incorporating hollow fibers with a membrane structure as described above to be damaged. ◎ On the other hand, γ-ray irradiation method However, if certain conditions are selected, it can be carried out safely without damaging the hollow fibers.

In the present invention, water or a liquid that is mixable with an aqueous solution and capable of penetrating into the pores inside the hollow fiber is passed through the hollow fiber, and then the hollow fiber is replaced with the liquid water or a water bath liquid to keep the hollow fiber in a wet state. Irradiate the r-light - In this case, the water is pure water. Yu, ii *
Akirabon hair? Water, □, Yusei.

Water containing inorganic salts such as saline, glycerin, etc.
Any liquid can be used as the aqueous solution of the material to be transported, or a liquid that can be mixed with the aqueous solution and can penetrate into the pores inside the hollow fiber, but alcohols such as methanol and ethanol are preferable. The radiation is irradiated from outside the separation device, but it is preferable to irradiate it after packaging, as this creates a more sanitary atmosphere◇ In this case, the preferred range of the irradiation device is: - Contamination - Enemies and Judging from the level of damage to the hollow fibers/X70 Ren[Gen~10X
10@ Roentgen, preferably CoXIO" Roentgen to 41X/0" Roentgen@ A polyethylene hollow fiber preferably used in the present invention can be manufactured by, for example, the following method.

ASTMD-/! 01 or more, preferably 0910 or more, and a melt index of 1 to 1S.
The highly oriented crystalline undrawn hollow fiber obtained by melt spinning at a spinning draft of 100 to 10,000 using a hollow fiber production nozzle in the temperature range of kN2/IC is processed as necessary to a &IJ mom one point or less. +0C after annealing at
3~-〇〇-cold stretching is carried out in the stretching region maintained as follows. Next, hot stretching is carried out in one stage or in multiple stages in the temperature range of Dao-tsoc. The amount is in the range of 50 to 900%, and after that, if necessary, heat set in a temperature range of 10 ON/3C, and after passing a liquid that can permeate into the pores inside the hollow fiber, 1all.
! ! The invention is characterized in that r-ray irradiation is performed with the hollow fibers in a wet state by replacing the body with water or aqueous ffj liquid.If r@ irradiation is performed under conditions without water or an aqueous solution, the hollow fibers will deteriorate severely, which is the present invention. It is not possible to achieve the purpose of
Preferable from the viewpoint of prevention.

The fractionating device W treated by the method of the present invention is extremely effective in treating body fluids, and is particularly suitable for separating plasma components from blood, further fractionating the separated plasma, and returning the active ingredients to the body. It shows the effect that it has. The present invention will be explained in detail in the following examples.□Example l W degree 0940. Melt Index! A hollow fiber obtained by bath melting Nogiri ethylene, with an inner diameter of 190 μm.
, film thickness: 3μ, average pore diameter by mercury porosimeter: a4
μ, porosity? The thickness of j% clofibril + 1M) is approximately 07μ, and the average length of the nodule in the fiber axis direction lk) is Q
μ, and the average width (dma) of micropores is a/≠p
The device shown in the figure was created using polyethylene hollow fibers. That is, ethanol was passed through the inside of the hollow fiber (41), which was equipped with outer lids (J) having an A 000 outlet at both ends, and water was immediately passed through the hollow fiber to fill it with water. After sealing the thus obtained separation device in a polyethylene bag, the axto@X-ray amount r! ! After sterilization by irradiation, the water in the cylindrical container was taken out and filtered through a membrane filter with a pore diameter of A that had been previously subjected to sterilization, and the filter was transferred onto an agar medium. No colonies were observed on the filter subjected to the culture test, and it was confirmed that the water in the cylinder was sterilized.There was no difference in the strength and elongation of the hollow fibers before and after the treatment.

[Brief explanation of drawings]

The figure shows a minute mti ethyle suitable for applying the method of the present invention,
, represents a hollow fiber.

Claims (1)

[Claims] In a separation device incorporating a porous expanded hollow fiber having micropores communicating with a wall surface, the interior of the hollow fiber can be mixed with water or a water bath liquid to the outside, and the pores inside the hollow fiber can be mixed with water or a water bath liquid. A liquid dispensing device characterized in that after passing a liquid that can permeate into the fiber, the liquid is directly replaced with water or a water bath liquid, and RLS irradiation is performed while the hollow fiber is in a wet state. ! 11 Law〇(k) Water or water f
The method described in Claim 1 JJ, characterized in that the liquid capable of mixing with the liquid and the liquid and capable of penetrating into the pores inside the hollow fibers is an alcohol.