JPS5851752B2 - Extraction method of meat aroma components - Google Patents

Extraction method of meat aroma components

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Publication number
JPS5851752B2
JPS5851752B2 JP51146302A JP14630276A JPS5851752B2 JP S5851752 B2 JPS5851752 B2 JP S5851752B2 JP 51146302 A JP51146302 A JP 51146302A JP 14630276 A JP14630276 A JP 14630276A JP S5851752 B2 JPS5851752 B2 JP S5851752B2
Authority
JP
Japan
Prior art keywords
meat
aroma
protease
beef
fat
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
JP51146302A
Other languages
Japanese (ja)
Other versions
JPS5386070A (en
Inventor
直樹 岩田
千香子 森岡
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ajinomoto Co Inc
Original Assignee
Ajinomoto Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Co Inc filed Critical Ajinomoto Co Inc
Priority to JP51146302A priority Critical patent/JPS5851752B2/en
Publication of JPS5386070A publication Critical patent/JPS5386070A/en
Publication of JPS5851752B2 publication Critical patent/JPS5851752B2/en
Expired legal-status Critical Current

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Description

【発明の詳細な説明】 本発明は肉類より効率的に香気成分を抽出する方法、更
に詳しくは調理条件で加熱処理した肉類の好ましい香気
を有する内置気成分の抽出法に関する。
DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for efficiently extracting aroma components from meat, and more particularly to a method for extracting aroma components having a desirable aroma from meat that has been heat-treated under cooking conditions.

従来、肉の香気は肉エキス中に共存する形で取得されて
来た。
Conventionally, the aroma of meat has been obtained by coexisting in meat extracts.

即ち肉エキスは獣肉、鳥肉、魚肉等の動物用を熱水抽出
した後、濃縮することにより得られて来た。
That is, meat extracts have been obtained by extracting animal meat, poultry meat, fish meat, etc. with hot water and then concentrating the extracted meat.

この従来法にあっては、肉類の香気成分は濃縮工程で大
部分が揮散してしまい香気に乏しいエキスしか得られな
いという欠点がある。
This conventional method has the disadvantage that most of the aroma components of the meat are volatilized during the concentration process, resulting in an extract lacking in aroma.

また、熱水で抽出される香気は肉類を咀明した時に感知
される香気とは同質ではなく、本従来法は本質的な問題
点を内蔵しているのである。
Furthermore, the aroma extracted with hot water is not the same as the aroma detected when meat is masticated, and this conventional method has an inherent problem.

一方、肉類の香気成分を有機溶剤で抽出する試みもなさ
れているが、必ずしも成功していない。
On the other hand, attempts have been made to extract aroma components from meat using organic solvents, but these efforts have not always been successful.

即チ、エチルエーテル、n−エキサン等の溶剤による抽
出は加熱処理した肉類を細砕して抽出するだげでは香気
成分の大部分は肉類組織中に残存して溶剤には僅かしか
移行して来ない。
For extraction with solvents such as ethyl ether, n-exane, etc., if the heat-treated meat is simply crushed and extracted, most of the aroma components will remain in the meat tissue and only a small amount will transfer to the solvent. do not come.

実験室規模ではエチルエーテル−エタノール、クロロホ
ルム−メタノール等の脱水溶剤を使用して抽出が試みら
れているが、この方法は抽出に長時間を要し、しかも香
気成分、脂質と同時に水分も抽出されて来るので溶剤の
回収が容易ではなく、大量処理には不適当である。
Extraction has been attempted on a laboratory scale using dehydrated solvents such as ethyl ether-ethanol and chloroform-methanol, but this method takes a long time to extract, and moreover, water is extracted at the same time as aroma components and lipids. It is difficult to recover the solvent and is therefore unsuitable for large-scale processing.

本発明者らは調理条件にて加熱処理した肉類、特に畜肉
、鳥肉の好ましい香気を有する内置気成分を簡便にして
安価に抽出する方法を確立すべく鋭意研究を行った。
The present inventors have conducted extensive research in order to establish a simple and inexpensive method for extracting internal air components having a favorable aroma from meat, particularly livestock meat and poultry, that have been heat-treated under cooking conditions.

先述した通り好ましい肉類の香気は水不溶性成分も関与
していることに着目し、本発明者らはこれらを水可溶性
成分と共に抽出する方法に関し検討した結果、溶剤によ
る香気成分の抽出は、肉の組織中にある蛋白質が香気成
分を保護していること及び同組織中の水分による溶剤の
内部への滲透の阻害により、円滑に進行しないことが判
明した。
As mentioned above, the present inventors focused on the fact that water-insoluble components are also involved in the desirable aroma of meat, and as a result of studying a method for extracting these together with water-soluble components, the inventors found that extraction of aroma components using a solvent does not affect the flavor of meat. It was found that the process did not proceed smoothly because the proteins in the tissue protected the aroma components and the moisture in the tissue inhibited penetration of the solvent into the interior.

本発明はこの知見に基き、それを更に発展させることに
より完成したものであって、肉類の蛋白質組織を破壊し
た後に脱水することによって肉の組織中に含まれている
香気を容易に油脂により抽出し得ることを見い出したの
である。
The present invention was completed based on this knowledge and further developed, and by dehydrating the protein tissue of meat after destroying it, the aroma contained in the meat tissue can be easily extracted with fats and oils. I discovered what could be done.

即ち、本発明は肉類、特に畜肉、鳥肉を煮熟成いは焙焼
等の加熱処理によって任意の香気を生じせしめ、これを
油脂で抽出する前であって、加熱処理の前又は後に細砕
状態にてグロテアーゼを作用せしめ肉の組織を形成する
蛋白質を部分分解する工程と、溶剤として油脂を使用し
肉組織中の水分を揮散させながら香気成分を抽出する工
程の組合せよりなり、本工程の組合せにより調理条件で
加熱処理した肉類の好ましい香気を有する内置味成分が
効率よく容易に得られるのである。
That is, the present invention produces a desired aroma by heat-treating meat, especially livestock meat, and poultry, such as boiling and aging or roasting, and before extracting this with oil or fat, and before or after the heat treatment. This process consists of a combination of a process in which grotease is applied in the crushed state to partially decompose the proteins that form the meat tissue, and a process in which aroma components are extracted while evaporating moisture in the meat tissue using fats and oils as a solvent. By this combination, it is possible to efficiently and easily obtain an internal flavor component having a preferable aroma of meat that has been heat-treated under cooking conditions.

本発明方法の第1段は、牛、豚、馬、羊、鶏、魚等の各
種原料肉、特に畜肉、鳥肉を生鮮状態で又は煮熟、焙焼
等の加熱処理を施した後、サイレントカッター、捕潰機
等の細砕機にて微小粒に細砕し、プロテアーゼ処理に付
すことにある。
In the first step of the method of the present invention, various raw meats such as cows, pigs, horses, sheep, chickens, and fishes, especially livestock meats and poultry meat, are subjected to heat treatment such as boiling or roasting in a fresh state or after being subjected to heat treatment such as boiling or roasting. It is crushed into fine particles using a crusher such as a silent cutter or crusher, and then subjected to protease treatment.

煮熟等の加熱処理を行った肉類を使用する場合には、そ
の除虫ずるドリップ等の肉汁液も同時に使用することが
香気の強さの点で有効である。
When using meat that has undergone heat treatment such as boiling, it is effective to use meat juice such as insect repellent drip at the same time in terms of the strength of the aroma.

プロテアーゼの使用量は酵素の種類及び処理条件にて異
るが、処理に長時間を要すると腐敗、酸化等の問題が生
ずることがあるので迅速に行うことが望ましく、通常原
料肉(より詳細には原料肉中の所謂肉質部分であって脂
質部分は入らない)1′?に対し100〜2000単位
で用いられる。
The amount of protease used varies depending on the type of enzyme and processing conditions, but if processing takes a long time, problems such as spoilage and oxidation may occur, so it is desirable to use protease quickly. is the so-called fleshy part of the raw meat, and does not include the fat part) 1'? It is used in units of 100 to 2000.

プロテアーゼは細砕肉とよく混合し、均一に分散した後
、通常室温乃至60℃に加熱してペースト状或いはかゆ
状になる迄蛋白質を部分分解する。
After the protease is thoroughly mixed with the minced meat and dispersed uniformly, it is heated to a temperature ranging from room temperature to 60°C to partially decompose the protein until it becomes a paste or gruel.

本反応は通常30分乃至3時間にて完結するが、分解程
度の許容範囲は広く、過度に分解が進行しても以後の処
理に格別支障はない。
This reaction is usually completed in 30 minutes to 3 hours, but there is a wide tolerance for the degree of decomposition, and even if the decomposition progresses excessively, there will be no particular hindrance to subsequent processing.

本グロテアーゼ処理は蛋白質の部分分解を目的としてい
るので、使用するプロテアーゼの種類には特に制限はな
いが、反応はpHを調整することなく行うのでpH6〜
7で活性の高い中性プロテアーゼ、例えばアスペルギル
スオリーゼからのプロテアーゼ、パパイン、バチルス属
菌からのプロテアーゼ等が好ましく用いられる。
Since the purpose of this grotease treatment is to partially decompose proteins, there are no particular restrictions on the type of protease used, but the reaction is carried out without adjusting the pH, so the pH is between 6 and 6.
Neutral proteases with high activity in 7, such as protease from Aspergillus oryse, papain, protease from Bacillus genus, etc., are preferably used.

プロテアーゼ処理に生鮮肉が使用された場合には必要に
より次いで調理香気を生せしめるため熱処理に付すので
あるが、以下述べる通り抽出工程にて熱処理を同時に行
うことも可能である。
When fresh meat is used for protease treatment, it is then subjected to heat treatment if necessary to produce a cooking aroma, but it is also possible to perform heat treatment simultaneously during the extraction process as described below.

次いで、分解処理した肉類は油脂類による抽出工程に付
される。
Next, the decomposed meat is subjected to an extraction process using fats and oils.

油脂類としては食用油脂なら如何なるものでもよく、牛
脂、豚脂等の動物脂或いはコーン油、大豆油、綿実油等
の植物油脂であってもよいが、使用量に相当する動’J
hMW、例えば牛肉にあっては牛脂等の使用が香気のバ
ランスの点で好適である。
The fats and oils may be any edible fats, such as animal fats such as beef tallow and pork fat, or vegetable fats such as corn oil, soybean oil, and cottonseed oil, but the amount of animal fats corresponding to the amount used may be
For hMW, for example beef, it is preferable to use beef tallow in terms of aroma balance.

また、油脂類の使用量は油脂及び原料肉の種類により異
るが原料の所謂肉質部に対し1.5乃至20倍量(重量
比)にて使用される。
The amount of fats and oils used varies depending on the type of fats and oils and raw meat, but is used in an amount (weight ratio) of 1.5 to 20 times the so-called fleshy part of the raw material.

従って原料肉牛に脂質部が入っている場合には外部より
加える油脂量は原料脂質部を考慮して決定すればよく、
場合によっては外部より全く油脂を加える必要のないこ
ともある。
Therefore, if the raw beef beef contains fat, the amount of fats and oils added from the outside can be determined by considering the fat content of the raw material.
In some cases, it may not be necessary to add any fat or oil from the outside.

さて、油脂類による抽出であるが、これは予め香気を加
熱処理して生成させている場合には、50乃至90℃に
て減圧下に肉牛の水分を徐々に揮発させながら30分乃
至1時間抽出するのが香気成分の散逸もなく効果的であ
る。
Now, regarding extraction with fats and oils, if the aroma has been generated by heat treatment in advance, this can be done for 30 minutes to 1 hour while gradually volatilizing the moisture of the beef under reduced pressure at 50 to 90°C. Extraction is effective without causing the aroma components to dissipate.

減圧の程度は実験的に決定できるが、通常100〜50
0關Hg程度である。
The degree of pressure reduction can be determined experimentally, but is usually 100 to 50
It is about 0 Hg.

一方、焙焼香に富む肉香気成分を得る目的のためには抽
出処理迄に加熱処理を行なわず、プロテアーゼ処理肉の
油脂による抽出を1000乃至120℃で30分乃至1
時間加熱することにより達成することができる。
On the other hand, in order to obtain a meat aroma component rich in roasted aroma, no heat treatment is performed before the extraction process, and the protease-treated meat is extracted with fat and oil at 1000 to 120°C for 30 minutes to 1 hour.
This can be achieved by heating for hours.

この場合香気の生成、脱水、抽出を一工程にて行い得る
ことになるので、一層簡略化されたプロセスにて優れた
香気付与物質が得られ効率的である。
In this case, generation of aroma, dehydration, and extraction can be performed in one step, so that an excellent aroma-imparting substance can be obtained through a more simplified process, which is efficient.

この様に抽出された香気成分は圧搾等の固液分離手段に
て肉残渣を除去して、油脂に含有された状態にて取得さ
れる。
The aroma components extracted in this way are obtained in a state contained in fats and oils by removing meat residue using solid-liquid separation means such as compression.

本発明により得られる肉香気成分は強い香気付与能を有
するものであって、種々の食品、調味料に応用すること
ができる。
The meat aroma component obtained by the present invention has a strong aroma imparting ability and can be applied to various foods and seasonings.

以下、実施例により本発明の詳細な説明する。Hereinafter, the present invention will be explained in detail with reference to Examples.

実施例 1 牛屑肉(赤身部約60%)iooyをミンチしてオーブ
ンにて250℃15分間焙焼した。
Example 1 Beef scraps (approximately 60% lean meat) iooy was minced and roasted in an oven at 250°C for 15 minutes.

その後、サイレントカッターで細砕し、プロテアーゼ(
天野製薬製「アマノA」、アスペルギルスオリーゼから
のプロテアーゼであって12000u/P)IPを水1
0rnlに分散させた後側えよく混合して40℃で1時
間酵素分解した。
After that, it was crushed with a silent cutter, and protease (
"Amano A" manufactured by Amano Pharmaceutical, protease from Aspergillus oryzae (12000u/P) IP in 1 part water
After dispersing in 0rnl, the mixture was thoroughly mixed and enzymatically decomposed at 40°C for 1 hour.

酵素分解後牛脂50yを加え、80℃、360mmHg
の条件下で攪拌しながら水分を微沸程度に揮散させなが
ら抽出を行い、水分の蒸気がは寸完了した時点(約1時
間)で終結した。
After enzymatic decomposition, add 50y of beef tallow and heat at 80℃, 360mmHg.
Extraction was carried out under the following conditions while stirring and evaporating water to a slight boiling point, and the extraction was terminated when the vapor of water almost completely disappeared (about 1 hour).

内容物を濾布を用いて圧搾濾過したところ、ローストビ
ーフ香に富む脂肪85グが得られた。
When the contents were compressed and filtered using a filter cloth, 85 g of fat rich in roast beef flavor was obtained.

実施例 2 牛スネ肉(赤身部約98%)10(lを2CII′L角
に切断し、これを20分間水100m1と共に蒸煮した
Example 2 10 liters of beef shank (approximately 98% lean meat) was cut into 2 CII'L cubes and steamed for 20 minutes with 100 ml of water.

蒸煮肉及びドリップをサイレントカッターに移し磨砕し
た。
The steamed meat and drippings were transferred to a silent cutter and ground.

以後、実施例1と同様に処理したところ、ボイルした牛
装置に富む脂肪45グが得られた。
Thereafter, it was treated in the same manner as in Example 1, and 45 g of boiled beef fat was obtained.

実施例 3 牛屑肉100f?を切断した後、サイレントカッターで
細砕し、これにプロテアーゼ(長潮産業製「ビオブラー
ゼ」、バチルス属菌より得られたプロテアーゼであって
50000u/P)0.1f?加え良く混練した。
Example 3 Beef scraps 100f? After cutting, pulverize with a silent cutter, and add 0.1 f? Add and mix well.

これを50℃にて1時間保持して酵素分解を行った。This was kept at 50°C for 1 hour to perform enzymatic decomposition.

分解後、牛脂100f?を加え良く混練後105℃にて
攪拌しながら油脂抽出した。
After decomposition, 100f of beef tallow? After adding and kneading well, oil and fat were extracted at 105° C. while stirring.

然る後、圧搾濾過して肉残渣を除去したところ、ロース
トビーフ香に極めて富む脂肪951が得られた。
Thereafter, when the meat residue was removed by compression filtration, fat 951 was obtained which was extremely rich in roast beef aroma.

実施例 4 豚バラ肉(赤身部約30%)LOOPを切断した後、サ
イレントカッターで細砕し、プロテアーゼ(科研化学製
「プロナーゼE」、70000u/P)0.1fを加え
て50℃1時間酵素分解した。
Example 4 After cutting pork belly meat (approximately 30% lean meat) LOOP, it was crushed with a silent cutter, 0.1f of protease ("Pronase E" manufactured by Kaken Chemical Co., Ltd., 70,000 u/P) was added, and the mixture was heated at 50°C for 1 hour. Enzymatically degraded.

次いで、105℃にて1時間加熱して、加熱処理、脂質
の分離、水分の揮散、並びに香気成分の抽出を同時に行
い、肉残渣を除去した。
Next, it was heated at 105° C. for 1 hour to perform heat treatment, separation of lipids, volatilization of water, and extraction of aroma components at the same time to remove meat residue.

その結果、ローストボーク香に富む脂肪671が得られ
た。
As a result, fat 671 rich in roasted balk flavor was obtained.

実施例 5 実施例1に於けるプロテアーゼの代りにパパイン0.5
2を使用した外は全く同様に操作したところ、香気に富
む脂肪65Pが得られた。
Example 5 Papain 0.5 instead of protease in Example 1
When the same procedure was carried out except that No. 2 was used, fat 65P rich in aroma was obtained.

実施例 6 市販牛肉(赤身部約50%)200fを水100WLl
にて20分間蒸煮した。
Example 6 200f of commercially available beef (approximately 50% lean meat) in 100WL of water
Steamed for 20 minutes.

蒸煮肉及びドリップをサイレントカッターに移し細砕し
、これにプロテアーゼ(「アマノAJ)0.5Pを加え
、良*;く混合して40℃1時間酵素分解した。
The steamed meat and drippings were transferred to a silent cutter and pulverized, 0.5 P of protease (Amano AJ) was added thereto, mixed well, and enzymatically decomposed at 40°C for 1 hour.

その後、湯浴温度60℃にて155 mmHgの条件下
に攪拌しながら水分を徐々に揮散させ、加水分離して来
た油脂に肉香気成分を抽出させた。
Thereafter, the water was gradually evaporated while stirring at a hot water bath temperature of 60° C. and 155 mmHg, and the meat aroma components were extracted from the hydrolyzed oil and fat.

水分の蒸発かほど完了した時点で抽出を終了し、内容物
を熱時圧搾濾過してボイルした牛装置に富む脂肪70グ
を得た。
The extraction was terminated when the water content was completely evaporated, and the contents were filtered under hot pressure to obtain 70 g of boiled beef fat.

一方、上記と同一の牛肉及びプロテアーゼを用いて特開
昭49−50164号明細書実施例2の方法(プロテア
ーゼ処理後、熱水抽出し抽出液を得る。
On the other hand, using the same beef and protease as above, the method of Example 2 of JP-A-49-50164 (after protease treatment, hot water extraction is performed to obtain an extract).

一方、残渣に水を加えて加熱し浮上する脂質を採取し、
該脂質を前記抽出液にその固形重量の3〜4重量%にな
る様に加え濃縮する方法)にて肉フレーバー含有エキス
を得た。
On the other hand, water is added to the residue, heated, and the floating lipids are collected.
A meat flavor-containing extract was obtained by adding the lipid to the above-mentioned extract in an amount of 3 to 4% by weight of its solid weight and concentrating it.

本発明に係る肉香気成分及び肉フレーバー含有エキスを
下表の処方に従って・・ンバーグに添加して、その内置
気付与効果の比較を尋問のパネル20名による官能検査
にて行った。
The meat flavor component and the meat flavor-containing extract according to the present invention were added to meat flavoring according to the recipe shown in the table below, and the aeration-imparting effects thereof were compared in a sensory test conducted by a panel of 20 interrogators.

結果は、本発明に係る肉香気成分の入った方がより内置
気が強いと判定した人数が16名、肉フレーバー含有エ
キスの入った方がより香気が強いと判定した人数が4名
で、その差は5%の危険率で有意であった。
The results showed that 16 people judged that the meat flavor component of the present invention had a stronger internal aroma, and 4 people judged that the meat flavor extract had a stronger aroma. The difference was significant with a hazard rate of 5%.

Claims (1)

【特許請求の範囲】[Claims] 1 生鮮又は加熱処理した肉類を細砕した後プロテアー
ゼにて部分分解し、次いで生鮮肉にあっては加熱処理し
た後又は加熱処理しつつ、徐々に水分を揮散せしめなが
ら油脂にて抽出することを特徴とする内置気成分の抽出
法。
1 Fresh or heat-treated meat is crushed, partially decomposed with protease, and then, in the case of fresh meat, after or while being heat-treated, water is gradually evaporated and extracted with fats and oils. Characteristic method for extracting internal air components.
JP51146302A 1976-12-06 1976-12-06 Extraction method of meat aroma components Expired JPS5851752B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP51146302A JPS5851752B2 (en) 1976-12-06 1976-12-06 Extraction method of meat aroma components

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP51146302A JPS5851752B2 (en) 1976-12-06 1976-12-06 Extraction method of meat aroma components

Publications (2)

Publication Number Publication Date
JPS5386070A JPS5386070A (en) 1978-07-29
JPS5851752B2 true JPS5851752B2 (en) 1983-11-18

Family

ID=15404595

Family Applications (1)

Application Number Title Priority Date Filing Date
JP51146302A Expired JPS5851752B2 (en) 1976-12-06 1976-12-06 Extraction method of meat aroma components

Country Status (1)

Country Link
JP (1) JPS5851752B2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2841043A1 (en) * 1978-09-21 1980-04-03 Maizena Gmbh Clear instant-soluble meat hydrolysate prepn. - by hydrolytic meat liquefaction with neutral protease of microbial origin
JPS5840063A (en) * 1981-09-01 1983-03-08 T Hasegawa Co Ltd Preparation of oily flavor prepared by heating

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Publication number Publication date
JPS5386070A (en) 1978-07-29

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