JPS5821544A - Measuring method for chemical reaction rate - Google Patents

Abstract

PURPOSE:To enable removal of a false signal due to dusts and air bubbles in a solution, by a method wherein an inclination of a change in absorbance is found from data by a minimum square method, and a second inclination is then found from data being within a width of a given range from aforesaid inclination. CONSTITUTION:An absorbance signal from a detector 1 is inputted to a central processing unit 5 through a logarithmic converting circuit 2, an A/D converting circuit 3, and an interface 4. The CPU5 finds a first inclination alpha' of a change in absorbance in a section from data of a whole range by a minimum square method, 40% worst is then removed from the value of the alpha' to select remaining data, and from the selected data, a second inclination alpha'' is further found by the minimum square method. The inclination alpha'' is multiplied by a given coefficient value to display an enzyme active value, being the measurements, in a display unit 11. Simultaneously, the whole section is divided into two sections of a front and a rear section to find inclination alpha''1 and alpha''2 in each section. If a ratio between the inclinations is within a given range, it is displayed to be normal, and if not, it is displayed to be abnormal

Description

DETAILED DESCRIPTION OF THE INVENTION The present WAFi absorbance changes in proportion to time in a chemical reaction. The present invention relates to a chemical reaction rate measuring method equipped with a means.

Conventionally, the II elementary reaction is known as an example of a chemical reaction in which absorbance changes in proportion to time. For enzymatic reactions, the enzyme activity value is determined by multiplying the amount of change per minute by a constant determined from the % substance and measurement conditions. There is. In the case of an enzymatic reaction with
There is a period of non-linear reaction called AIDS, and a linear reaction occurs after a 0-hour delay from the start of the reaction, so the enzyme activity value must be determined by the linear portion of this line.7For this reason, conventional methods have been I found out the time of the aforementioned Lag 7 Aime and that time had passed 1lKI]j! ! Alternatively, as disclosed in QIi Kai Publication No. 54-40036, whether or not the measurement result is a straight line is determined by calculating the integral value of the change in absorbance O at two points in time. method is used.

However, in the latter method, all the data in the integral range are included, and the data in the reaction solution are calculated.

Since false signals 4 caused by air bubbles, sample Kll, and the resulting turbidity are included, it often happens that th is determined to be a straight line even if it is actually a straight line. On the other hand, in these reaction chambers, electricity, bubbles, and turbidity from the sample
It is virtually impossible to completely remove * from the reaction solution, therefore, in order to obtain a highly reliable measurement result, the accuracy of K is the #! of the running signal. & Management methods are required to maximize the effects of measurement error sources.

In view of the above-mentioned point, the purpose of the present invention is to provide a method for processing measurement signals that can eliminate the effects of measurement errors, that is, the method for measuring chemical reaction rates according to the present invention is based on photoelectric conversion. The signal of nine luminous intensity is subjected to M/D conversion, and the digital signal is taken into the fixed interval memory function at regular time intervals, and then) the data is converted into O intervals by the least square method. The method is characterized in that the first gradient of the change in absorbance is found, the data within a certain range of the city are selected for the 10th gradient, and the 20th gradient, - is found from the selected data. It is.

In addition, when measuring an item resulting from a new reaction in an enzyme reaction, it is necessary to determine in advance how long the period of a nonlinear reaction called lag 7 AIDS is and how long the period of a linear reaction is.

If we know the time and measure the term l due to the same reaction, it becomes possible to measure the times of those sections in advance. In order to do so, we can continuously calculate the 10th slope of the absorbance change for each section using the least squares method for each section. Obtain the tilting device, and display this 2nd t) tilt over a number of continuous intervals in each constant interval, and depending on whether the value exists in a certain range 111MK, determine the interval and proportionality where the absorbance and time maintain a proportional relationship. The method for determining the interval where the relationship does not hold is 4o, and when expressed as the tilt tube activity value in the interval where absorbance and time maintain a proportional relationship, In order to obtain highly reliable values by removing data tubes caused by air bubbles, turbidity caused by sample KIE, etc., data within a certain city is calculated using the least squares method. Find the 20 tilt configuration using
The present invention provides a method for displaying the activity value as an activity value.

In addition, the activity value was originally determined as the section of the tm* part of the part A where the change in absorbance was non-linear, or by the least squares method). 10 Using data in a certain city from the power distribution To check that the data that should have been taken is discarded, divide the section where the data was taken into two sections, the front and back, and check the whole section. Apart from the slope of section O, the absorbance change in the two sections O second slope α1. a, respectively, and calculate their ratio, and if the value falls within a certain condition (ideally 1), it will appear to be normal, but it will be 1kl/%, which is a method to display an abnormality.

Hereinafter, the present invention will be explained in more detail along with the embodiments tmwec. Figure 1 is a schematic diagram of the equipment used to carry out the Hiromoto method.
1 is a detector for measuring absorbance.

After the absorbance signal from the detector 1 passes through the logarithmic conversion circuit 2,
Anada/Digital conversion circuit 3 converts the digital signal to /D
converted. The ζO digital signal is input to the CPU (electronic computer operating system) S through the interface 4, where the O operation described below is performed. 8 is 8 points M,
Absorbance slope 11! Required for measuring the length of the required section, the specified value of repeated training O, etc.! The conditions are memorized. This 8 point Ml
Writing to OII is performed through the CPU 5 via the digital interface 7 and the 8th 6 by operating the command switch 7. Also, reference numeral 9 is a ROM, which has a built-in arithmetic algorithm for the CPUK. In this manner, in response to the input of the absorbance signal, the CPU 5 performs calculations, and the results are displayed on the display device 11 via the interface 10, indicating whether or not the measured section is in a straight line.

#I2 is a diagram showing the relationship between the absorbance spectrum and the measurement process, and FIG. 3 is the arithmetic processing by the CPU 5K.

The measurement method will be explained below with reference to charts showing the main parts.

In FIG. 2, 12 is the absorbance spectrum, 0 indicates the measurement start point, and 8 indicates the end point.

As shown in the figure, the reaction in the first certain section of the % spectrum is not linear, and the absorbance begins to decrease linearly.

The start of the delay time DTfeff calculation process corresponding to this non-linear interval is delayed. This corresponds to the 13-day delay timer shown in FIG. Measurement started when entering the direct section, 1111th fixed section MTs
Absorbance data is taken from snow K11 in the second measurement section and sent to calculation processing! , e01i11#j1811
0141ffijlf-1! - Corresponds to F.

If the linear section of the t-ra luminous intensity change is known in advance in ζζ, then the 1110 [i11 section O gold range @M
To find the first tilt position t of the interval K by the method of least squares), then at) at) @fD
Worst 40% of them! # Remove it and select the remaining 0 data), from this eaas data, IK # Bo by the method of least squares.
0 to find the nod distribution a, and ζO inclination K constant coefficient 't#
The enzyme activity value, which is the measured value, is displayed.

In addition, in parallel to this, the entire ζO section is divided into two equal parts, the front and back, and the O sections Mrt and Mrm are also divided into the entire section MT and 1
Find the 10th tilt arrangement around a straight line and i by the least squares method on Iill*, and use the worst 4 (ltlk%/%9 data tube) located on the straight line - Calculate #[#1 using the multiplicative law. Then, if the O ratio α is within a certain range and is not normal, an abnormality will be displayed. In other words, if the O ratio α is within a certain range, an abnormality will be displayed. If the inclination ratio does not satisfy the relationship Oj9□/accumulation, ≦IJt),
After the third diagram, an abnormal signal indicating that abnormal data is included in the measurement section is displayed on the display section.

On the other hand, if the linear section of the absorbance change is determined by the term iitw+ due to an unknown new reaction, determine αtm for each section using the method described above for multiple sections that continued over a preset measurement time and compare them. A non-straight section and a straight section O are determined and determined, and this straight section can be used as a straight section for measuring the pipe activity value.

As described above, according to the present invention, the least squares method is used to derive the direct at, and data in the middle of a certain range from the inclination is used to further calculate the second inclination. Since the active and healthy display is performed using the ays in the solution,
By removing false signal tubes due to air bubbles, etc., a highly reliable value is obtained, and by calculating the ratio of the second slope using the data divided into two sections before and after the first, it is possible to display the normal/abnormal O judgment. It has the advantage of being able to check the data. Another advantage is that when measuring a new item, it is possible to determine how long the linear and non-linear regions are.

[Brief explanation of the drawing]

Figure 1 is a schematic diagram of an apparatus for carrying out the method of constant chemical reaction rate according to the present invention, Figure 2 is a diagram showing the relationship between the absorbance program for measuring reaction rate ft and the measurement procedure, and Figure 3 is a diagram showing the relationship between the absorbance program for measuring the reaction rate ft and the measurement procedure. FIG. 3 is a chart diagram showing the main parts of arithmetic processing. 1... Detection II 2-... Logarithmic conversion circuit 3
...Anajig/digital conversion circuit 4.6.10...Interface 5...CPU 7...Command switch 8.
...Bonmu M 9-ROMll...Display device 12...Absorbance spectrum Applicant: JASCO Corporation Agent Yukio Maruyama

Claims (1)

[Claims] (1) In a chemical reaction rate measuring method in which the reaction rate when a proportional relationship between absorbance and time is maintained is determined from this slope, the photoelectrically converted absorbance is subjected to signal tube M/D conversion, Incorporating data at regular time intervals among the digital signals into a fixed section review function, and then calculating the tenth gradient t of the absorbance change that applies to that section from the internal data using the least squares method; (
c) Demet located in the middle thickness of a certain range from the 10th tilted arrangement
A chemical reaction constant method whose 41st image is to obtain the 20th slope α from the selected data. (2. Using the method described in Claim I! Section 1. For each continuous section, calculate and display the second slope al for each section, and from this value, calculate the absorbance and time. A method for determining an interval in which a proportional relationship is maintained and an interval in which the proportional relationship does not stand. (3) An interval in which the absorbance determined using the method set forth in claim 2 and time maintain a proportional relationship for 1 m. , the above-mentioned second tilting device in the 0 interval is determined by setting this interval as a new constant interval, and the measured value is displayed by multiplying by a constant constant tS. ) 411 Request t) @8 For the section to be measured and displayed using the method described in Section 3, the absorbance change between the front half section and the rear cattle section of the section is determined by the second slope. 10 Methods for measuring chemical reactions with mold.