JPS58150503A - Plant disease-combatting agent - Google Patents

Abstract

PURPOSE:The titled combatting agent that contains SF-1917 substance as an active ingredient, showing high combatting effect against rice sheath blight and cucumber Anthracnose without toxicity to men, animals and fishes, causing no chemical injury to crops. CONSTITUTION:A microorganism in Micromonospora, which has shown only weak antifungal activity against plant disease causative microorganisms in the antifungal tests on agar media, is cultured to give SF-1917 substance of the formula. The product or its salt is used to test its combatting effect against plant diseases with plants and found that it shows high combatting effect in a much lower concentration than the minimum concentration in the above agar test inhibiting the growth of the plant diseases-causative microorganisms. Thus, its use has been established as a plant disease combatting agent such as fungicide for agricultural and horticultural purposes. The combatting agent is used by spraying it on crop foliage as well as by applying to water surface, into water or to soil surface or into soil.

Description

[Detailed description of the invention] [! BACKGROUND OF THE INVENTION The present invention is directed to the use of 5F-1917 substance or its acid addition salt.

More specifically, the present invention provides antibiotic 5F-1917@
The present invention relates to a conventional disease control agent containing negative or an acid addition salt thereof as an active ingredient.

j#t (E) It has been pointed out that the antibiotic lll 1-member agent and synthetic compound 1 agent used as rice strangle control agents lack medicinal efficacy, and there are great concerns about continued use of il!L in the future. It is used as a preventive medicine for five diseases, but the active ingredients include X-Jakuho compound, which makes it difficult to use as a pesticide or other chemicals. Since it is sometimes a social problem due to its environmental pollution and toxicity to humans and animals, there is strong demand from all over the world for an accurate pest control agent.

(If) Cost of the Invention The present invention is based on the antibiotic 5F-191? The inventors of the present invention aim to solve the above points using substances.
While searching for substances effective against plant diseases, we discovered that there was a substance in the cultured wastewater of microorganisms belonging to the genus Micromonospora that was effective against rice sheath blight. did. In tests using plants, this quality
Although it showed a strong control effect, it showed almost no anti-effect on agar medium. Therefore, this substance was purified and isolated by the Kubo test using plants, and the active ingredient of I1 was used in the same manner as 5F-1917.

5F-1917@buy and its ffi manufacturing method are described in a known document, Japanese Publication Publication M53-38697.

5F-1917 has the following chemical structure.

This document describes nine amO filamentous fungi, including plant pathogenic filamentous fungi.
The antibacterial spectrum of 8F-1917@ has been studied, but its antibacterial effect is weak and almost non-existent. Furthermore, no studies have been conducted regarding the effect of controlling plant diseases. Furthermore, the inventors conducted a separate test with 9 resistances, and 8F.
-19171m! 8F-19179 has a weak anti-effect against plant pathogenic filaments, and the usefulness of 8F-19179 as a plant disease control agent cannot be inferred from the general anti-effect. However, the 8F-1917 substance, which the Ministry of Agriculture, Labor and Welfare has conducted using plants to prevent plant disease, is much more effective than its ability to prevent plant pathogens from slow growth. It has shown excellent effectiveness in controlling 411 insect bites with a low level of 1IIIL. As described above, the present inventors discovered a new use of the 5F-1917 substance as a plant disease control agent and its excellent efficacy for the first time through pest control tests conducted using plants, thereby completing the present invention.

The 5F-191791 substance of the present invention generally exhibits control activity against a wide range of plant diseases, but it has excellent control effects on rice sheath blight on bone, b, and cucumber charcoal stain b. 5F-1917 substance Hiro, extremely low toxicity antibiotic 1
It is of high quality and has absolutely no toxicity to humans, livestock, or fish, and the prefectural police cannot be held responsible for cropping. Therefore, it is considered to have sufficient activity to compensate for the drawbacks of the existing drugs mentioned above.

(In) Specifics of the invention! 1) 5F-191711131 [O41L 5F-1917g tiger according to the present invention as a proboscis disease control agent,
8F-19 has a control effect against plant diseases.
17# In fact, it can also be used as a hoe-cane disease prevention agent, such as a hoeing agent for adding salt to the ridges of the t-1k-tension.

The plant disease control agent according to the present invention contains the above-mentioned 5F as an active ingredient.
- 1917 substance is an addition salt of 11t
Except for the above, the d1-rIA tonic agent can take any form or manner of use that can be specially used as a pesticide.

Specifically, for example, the 5F-1917 substance of the present invention or its rib addition salt may be used as is or diluted with water, solid powder, or other suitable carrier, and if necessary, an auxiliary agent such as a spreading agent may be added. Use it in your mouth, or mix it with the carcass donated body or a solid carrier by a method commonly used in agriculture and manufacturing, and if necessary, add a weaving agent, a spreading agent, a dispersing agent, and an emulsifying agent. By adding adjuvants such as fixing agents and lubricants, it can be used in various formulations such as aqueous preparations, solutions, emulsions, l&p, granules, and granules.

In producing these preparations, liquid carriers include:
A substance that serves as a solvent for the 5F-1917 substance or its rib addition salt of the present invention, or a substance that can be dispersed or dissolved with an auxiliary agent is used. For example, water, aromatic hydrocarbons, aliphatic hydrocarbons, alcohols, esters, dimethylformand, dimethylforphosite, etc. with large ketone-1a properties, and solid carriers such as mori, clay, kaolin, etc. Talc, gravel, bentonite, calcium carbonate, powder and granules can be used. Examples of adjuvants include nonionic, anionic, cationic, and amphoteric surfactants, ligninsulfonic acid or its salts, gums, iron salts, methyl cellulose, etc.

The pesticidal agent according to the present invention can be used by spraying on a single group of crops, and can also be used by spraying on the surface of water, in water, in the direction of soil, or during soil destruction.

Compatible jl&-horticultural agents or fertilizers can be mixed on the spot. Other l&-horizontal agents include, for example, monocidal agents, insecticides, herbicides, plant growth agents, and the like.

When the plant disease control agent of the present invention is used as a liquid agent, it is desirable that the 5F-1917 substance of the present invention be contained in the Tsutomi kifu liquid in an amount of IO to 11,000 pp. In the case of spraying, aircraft spraying, etc., it can be used as a spraying liquid with a thicker chain if necessary), and when used as a powder, granule, granule, etc., 0.
It is desirable to include 1 to 30 pieces.

The application amount is based on the type of target disease and Ii & aI of the target crop.
Although it varies depending on the type, application mode, etc., an example of applying it to soil is 10% of the active ingredients of the comb wettable powder.
-), for example, 50 to 200 liters; for an aqueous solution (10% active ingredient), for example, 50 to 200 liters;
If it is a granule (active ingredient 5-), nine to six is 2 to 6, and a powder (active ingredient 5-).
If the active ingredient is 2-) then the analogy is 2-6:41! Direct application rate is generally excessive.

2) Experimental Examples The present invention is not limited to the following examples, and it goes without saying that many modifications and modification means not exemplified here may be employed.

Plant disease control agent of the 5F-1917 substance of the present invention For example, the following are examples.

Formulation example 1 Wettable powder 1, mold part 5F-1917 substance 20 clay
10 Gravel soil
, 5 lignin sulfone relatives 3 polyoxyethylene alkyl allyl ether 2
The above ingredient qualifications - uniformly pulverized and mixed are the active ingredients 2
O-t-I borrow a hydrating agent.

Formulation Example 2 Water 1ll Parts by weight of I agent 5F-1917 substance hydrochloride 1G polyoxyethylene/alkyl allyl ether 2 Water
By mixing and dissolving the ingredients in 1 of 98, an aqueous solvent containing active ingredient 10- is obtained.

Formulation Example 3 Granule Weight Parts 5F-1917 Substance 5 Clay
92 Carboxymethyl cellulose Mix the above three ingredients, add varying amounts of water, and mix.t11.
If dried after 1J11, granules rich in active ingredient 5- can be obtained.0 1111j? i14 m agent weight part 5F-19t7115 JiL2 stearin iron calcicalcium 1 anhydrous silicon powder 1 clay 48 talc
48 If the above ingredients are uniformly ground and mixed, a powder containing 2% of the active ingredient can be obtained.

佽) Medicinal efficacy area acclimatization test example 1 Rice sheath blight control effect test 11500G The above formulation example 2 was added to a water pail (Shinanasu "Jukoku") grown in a Wagner pot at the hawthorn stage. The aqueous solvent was added to a predetermined m degree of ridge solution, and it was sprayed at a rate of 70 m/3 pots using a spray gun (2kt'ai). Immediately after air-drying, 4-9 chichichi blight was cultured on a peptone-added potato broth agar medium for 48 hours and punched out using a cork pole with a diameter of 0.5 tym. We held a ceremony at a distance of 15 cm from the ground.

Each tank was covered with a vinyl cylinder to prevent the invasion of sheath blight by 11b, and the temperature was 30℃ during the day and 24℃ at night.
The disease was allowed to develop by leaving it in a glass greenhouse at ℃, and after 10 days of treatment, the lesion length of #4 grave was measured, and the control value was calculated using the following formula. At the same time, the occurrence status of i-9 drug damage was determined by **.

Average sting length in untreated area The results are shown in the table below and were 9A.

Test Example 2 Chie cucumber seedling damping-off control test Chie cucumber seedling damping-off fungi were cultured on a hot potato decoction agar medium,
A contact source was created by mixing and grinding with three times the amount of rice. Using cucumber (variety Tokiwa Jizo) as a test crop, 20 sprouted seeds/bot were sown and covered with soil in a 115,000 Ahl Wagner pot filled with MliI field soil, and the above inoculum and killed #i field soil were sown and covered with soil. Inoculation was performed by uniformly scattering a mixture of equal amounts on top of the mixture. After vaccination,
After 48 hours of filtration in a greenhouse at 28°C, the irrigation agent prepared according to Formulation Example 1 was prepared at a prescribed 11fK and used as an end-injection solution.
Pour 100ml of this chemical solution per pot evenly onto the ground surface using a pipette.9. After that, in order to facilitate the invasion and progress of P. III, the plants are placed in a glass greenhouse at a temperature of 30°C to 28°C, and the humidity of the soil in the pot is kept slightly dry to allow the disease to develop. What about the investigation? The number of germination and the number of whole shaded ridges until after 3rd period of II.

Test sample 3 Cucumber JR scar disease control effect test The water produced according to Formulation Example 2 using medium-sized cucumber seedlings (cultivar Tokiwa Jiori) of the second business development period, which were grown in three pots in a No. 3 clay pot. Make sure to take the medicine at the prescribed time! Open the liquid and use a spray gun to spray 40 liters of 'L 3 wafers, let it dry and place it in a silver base at 24°C to suspend the conidia of melon anthracnose. Inoculate by spraying the turbid liquid evenly, keep it at night, and then move it to an artificial climate room to induce disease. After 15 days of contact, the degree of disease onset is 0KO1lfi. An index of 1 is given to those with 1 to 5 diseased furrows, and an index of 2 is given to those with 6 to 15 diseased furrows.
16 to 30 things with an index of 3, 31 to 50 illll
A survey was conducted by giving 40 finger ridges to those with 51 or more ridges, and a 5ON number to those with 51 or more, and the control value was calculated using the following formula.

The results were as shown in the table below.

ε) H indicates that there is no prefecture shoe.

Test Example 4 Antibacterial test against plant pathogens Moths The plant pathogens shown in the table below were tested, and the presence or absence of growth of one line on an agar plate was examined, and moth growth inhibition 11f (MIC) was conducted.
I asked for That is, the 8F of the present invention is added to a potato decoction agar medium.
- Create a dilution series by incorporating 1917 purchase, Shah V
K was poured in and solidified to prepare an agar plate. The test bacteria were inoculated onto the agar plate and incubated at 25°C for 72 hours.
After 1 ml, the presence or absence of growth of the test bacteria was observed.

The results are shown in the table below, and it was found that the 5F-1917 substance had a weak anti-inflammatory effect against pathogens.

Procedural amendment July 20, 1980 Kazuo Wakasugi, Commissioner of the Patent Office, 1982 Patent No. 34395 2, Name of invention Plant disease control agent 3, Person making the amendment Relationship to the case Patent applicant address Name ( 609) Meiji Seika Co., Ltd. 4, Agent fl+ Corrected "Shibori" in line 14, page 1 of the specification, to read "crest."

(2) 2nd line, page 3 (3) 2nd line on page 6 of the same page rPyricnlariaJ Corrected as rPyriculariaJ.

Claims (1)

[Claims] 1. A plant disease control agent containing a 5F-1917 substance or an acid addition salt thereof as an active ingredient.