JPS5745456A - Method for photographic-chemical immunoassay - Google Patents

Method for photographic-chemical immunoassay

Info

Publication number
JPS5745456A
JPS5745456A JP12059580A JP12059580A JPS5745456A JP S5745456 A JPS5745456 A JP S5745456A JP 12059580 A JP12059580 A JP 12059580A JP 12059580 A JP12059580 A JP 12059580A JP S5745456 A JPS5745456 A JP S5745456A
Authority
JP
Japan
Prior art keywords
antigen
dye
film
antibody
reacted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP12059580A
Other languages
Japanese (ja)
Other versions
JPS6223825B2 (en
Inventor
Masaki Okazaki
Yoshiji Shiyouda
Yoshiro Kumano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Holdings Corp
Original Assignee
Fuji Photo Film Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fuji Photo Film Co Ltd filed Critical Fuji Photo Film Co Ltd
Priority to JP12059580A priority Critical patent/JPS5745456A/en
Priority to DE8181106822T priority patent/DE3170134D1/en
Priority to EP81106822A priority patent/EP0047470B1/en
Publication of JPS5745456A publication Critical patent/JPS5745456A/en
Priority to US06/506,225 priority patent/US4473652A/en
Publication of JPS6223825B2 publication Critical patent/JPS6223825B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/583—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with non-fluorescent dye label

Abstract

PURPOSE:To easily label an antigen or an antibody by using mero-cyaine dye as a labeling agent having substituents including carboxyl group in the heterocyclic ring. CONSTITUTION:A merocyanine dye which has substituents including carboxyl group in the heterocclic ring as shown in the formula, is added to insulin and reacted to label the resulting product. The labeled antigen thus obtained is reacted immunochemically, and the product is dropped on the film painted with unexposed AgBrCl, then exposed to the light of wavelength which is absorbed by the dye. After developing this film, the blackening density on the film obtained by fixing, washing with water and drying according to the ordinary procedure, is measured by photographic densitometer. According to this process, an antigen or an antibody is easily labeled and the trace of them is detected.
JP12059580A 1980-09-02 1980-09-02 Method for photographic-chemical immunoassay Granted JPS5745456A (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP12059580A JPS5745456A (en) 1980-09-02 1980-09-02 Method for photographic-chemical immunoassay
DE8181106822T DE3170134D1 (en) 1980-09-02 1981-09-01 Method for immunochemical measurement
EP81106822A EP0047470B1 (en) 1980-09-02 1981-09-01 Method for immunochemical measurement
US06/506,225 US4473652A (en) 1980-09-02 1983-06-22 Method and immunochemical measurement

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP12059580A JPS5745456A (en) 1980-09-02 1980-09-02 Method for photographic-chemical immunoassay

Publications (2)

Publication Number Publication Date
JPS5745456A true JPS5745456A (en) 1982-03-15
JPS6223825B2 JPS6223825B2 (en) 1987-05-25

Family

ID=14790144

Family Applications (1)

Application Number Title Priority Date Filing Date
JP12059580A Granted JPS5745456A (en) 1980-09-02 1980-09-02 Method for photographic-chemical immunoassay

Country Status (1)

Country Link
JP (1) JPS5745456A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04314828A (en) * 1990-12-28 1992-11-06 Kobe Steel Ltd Production of high strength hot-dip galvanized steel plate excellent in workability

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS55116259A (en) * 1979-03-01 1980-09-06 Fuji Photo Film Co Ltd Microimmunoassay method

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS55116259A (en) * 1979-03-01 1980-09-06 Fuji Photo Film Co Ltd Microimmunoassay method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04314828A (en) * 1990-12-28 1992-11-06 Kobe Steel Ltd Production of high strength hot-dip galvanized steel plate excellent in workability

Also Published As

Publication number Publication date
JPS6223825B2 (en) 1987-05-25

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