JPS5745455A - Method for photographic-chemical immunoassay - Google Patents

Method for photographic-chemical immunoassay

Info

Publication number
JPS5745455A
JPS5745455A JP12059480A JP12059480A JPS5745455A JP S5745455 A JPS5745455 A JP S5745455A JP 12059480 A JP12059480 A JP 12059480A JP 12059480 A JP12059480 A JP 12059480A JP S5745455 A JPS5745455 A JP S5745455A
Authority
JP
Japan
Prior art keywords
antigen
film
group
antibody
reacted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP12059480A
Other languages
Japanese (ja)
Other versions
JPS6228425B2 (en
Inventor
Masaki Okazaki
Yoshiji Shiyouda
Yoshiro Kumano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Holdings Corp
Original Assignee
Fuji Photo Film Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fuji Photo Film Co Ltd filed Critical Fuji Photo Film Co Ltd
Priority to JP12059480A priority Critical patent/JPS5745455A/en
Priority to EP81106822A priority patent/EP0047470B1/en
Priority to DE8181106822T priority patent/DE3170134D1/en
Publication of JPS5745455A publication Critical patent/JPS5745455A/en
Priority to US06/506,225 priority patent/US4473652A/en
Publication of JPS6228425B2 publication Critical patent/JPS6228425B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/583—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with non-fluorescent dye label

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Silver Salt Photography Or Processing Solution Therefor (AREA)

Abstract

PURPOSE:To easily label an antigen or an antibody, by using a cyanine dye which contains substituents including a corboxyl group in the terminal heterocyclic ring as a labelling material to form a cyanine chromophoric group. CONSTITUTION:A cyanine dye contg. substituents including a carboxyl group attached to the atom except nitrogen in the terminal heterocyclic ring of a chromophoric group, is added to insuling and reacted to label the resulting product. The labeled antigen thus obtained is reacted immunochemically, and the reaction product is dropped on the film coated with unexposed AgBrCl, then exposed to the light of wavelength that is absorbed by the dye. After developing this film, it is fixed, washed with water and dried according to the ordinary procedure, then the blackening density on the film is measured by a photographic densitometer. According to this procedure, an antigen or an antibody is easily labeled and the trace of them is detected.
JP12059480A 1980-09-02 1980-09-02 Method for photographic-chemical immunoassay Granted JPS5745455A (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP12059480A JPS5745455A (en) 1980-09-02 1980-09-02 Method for photographic-chemical immunoassay
EP81106822A EP0047470B1 (en) 1980-09-02 1981-09-01 Method for immunochemical measurement
DE8181106822T DE3170134D1 (en) 1980-09-02 1981-09-01 Method for immunochemical measurement
US06/506,225 US4473652A (en) 1980-09-02 1983-06-22 Method and immunochemical measurement

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP12059480A JPS5745455A (en) 1980-09-02 1980-09-02 Method for photographic-chemical immunoassay

Publications (2)

Publication Number Publication Date
JPS5745455A true JPS5745455A (en) 1982-03-15
JPS6228425B2 JPS6228425B2 (en) 1987-06-19

Family

ID=14790121

Family Applications (1)

Application Number Title Priority Date Filing Date
JP12059480A Granted JPS5745455A (en) 1980-09-02 1980-09-02 Method for photographic-chemical immunoassay

Country Status (1)

Country Link
JP (1) JPS5745455A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5438736A (en) * 1993-04-30 1995-08-08 Yoshida Kogyo K.K. Swivel hook
DE102017008086A1 (en) 2016-09-09 2018-03-15 Ngk Insulators, Ltd. gas sensor

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS55116259A (en) * 1979-03-01 1980-09-06 Fuji Photo Film Co Ltd Microimmunoassay method

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS55116259A (en) * 1979-03-01 1980-09-06 Fuji Photo Film Co Ltd Microimmunoassay method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5438736A (en) * 1993-04-30 1995-08-08 Yoshida Kogyo K.K. Swivel hook
DE102017008086A1 (en) 2016-09-09 2018-03-15 Ngk Insulators, Ltd. gas sensor

Also Published As

Publication number Publication date
JPS6228425B2 (en) 1987-06-19

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