JPS57204454A - Separating material, separator and separating method of t cell - Google Patents

Separating material, separator and separating method of t cell

Info

Publication number
JPS57204454A
JPS57204454A JP56088834A JP8883481A JPS57204454A JP S57204454 A JPS57204454 A JP S57204454A JP 56088834 A JP56088834 A JP 56088834A JP 8883481 A JP8883481 A JP 8883481A JP S57204454 A JPS57204454 A JP S57204454A
Authority
JP
Japan
Prior art keywords
cell
liquid
separator
separating material
separating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP56088834A
Other languages
Japanese (ja)
Inventor
Tsutomu Abe
Tsutae Akao
Akihiko Ikeda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Asahi Kasei Corp
Asahi Chemical Industry Co Ltd
Original Assignee
Asahi Chemical Industry Co Ltd
Asahi Kasei Kogyo KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asahi Chemical Industry Co Ltd, Asahi Kasei Kogyo KK filed Critical Asahi Chemical Industry Co Ltd
Priority to JP56088834A priority Critical patent/JPS57204454A/en
Publication of JPS57204454A publication Critical patent/JPS57204454A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

PURPOSE:To obtain a T cell in high purity and high yield without giving an immune stimulus to the cell, by using a water soluble hydrophobic solid having the porous surface as a selective separating material of T cell. CONSTITUTION:A T cell separator is a column 3 provided with an entrance 1 and an exit 2 of a leukocyte floating liquid and washing liquid in which a filter 4 having a mean pore diameter not flowing out a T cell separating material is attached to the exit 2 and the T cell separating material 5 consisting of a hydrophobic, water insoluble and porous granular solid having >=500Angstrom and <5,000Angstrom mean pore diameter and having >=40mu and <75mu grain diameter in water, is filled up in the column 3. In order to separate the T cell from the leukocyte, the leulocyte prepared from blood is floated in the liquid containing >=2g/dl protein at first. Said liquid is poured into the separator and an outflow is stopped at a point of time of finishing the permeation to the material 5 and it is left as it is for a fixed time. Hereafter, the T cell is separaed by washing out unadhering cell by flowing the washing liquid.
JP56088834A 1981-06-11 1981-06-11 Separating material, separator and separating method of t cell Pending JPS57204454A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP56088834A JPS57204454A (en) 1981-06-11 1981-06-11 Separating material, separator and separating method of t cell

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP56088834A JPS57204454A (en) 1981-06-11 1981-06-11 Separating material, separator and separating method of t cell

Publications (1)

Publication Number Publication Date
JPS57204454A true JPS57204454A (en) 1982-12-15

Family

ID=13953969

Family Applications (1)

Application Number Title Priority Date Filing Date
JP56088834A Pending JPS57204454A (en) 1981-06-11 1981-06-11 Separating material, separator and separating method of t cell

Country Status (1)

Country Link
JP (1) JPS57204454A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0240824A2 (en) * 1986-03-22 1987-10-14 Toa Nenryo Kogyo Kabushiki Kaisha Cell separator and process of producing the same
US5085781A (en) * 1989-02-28 1992-02-04 Asahi Kogaku Kogyo Kabushiki Kaisha Separating agent, separator and method of separating cell or virus

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5436963A (en) * 1977-08-29 1979-03-19 Furukawa Electric Co Ltd:The Gauge for manufacturing bend pipe with flange

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5436963A (en) * 1977-08-29 1979-03-19 Furukawa Electric Co Ltd:The Gauge for manufacturing bend pipe with flange

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0240824A2 (en) * 1986-03-22 1987-10-14 Toa Nenryo Kogyo Kabushiki Kaisha Cell separator and process of producing the same
US5085781A (en) * 1989-02-28 1992-02-04 Asahi Kogaku Kogyo Kabushiki Kaisha Separating agent, separator and method of separating cell or virus
USRE35267E (en) * 1989-02-28 1996-06-11 Asahi Kogaku Kogyo Kabushiki Kaisha Separating agent, separator and method of separating cell or virus

Similar Documents

Publication Publication Date Title
JPS55109459A (en) Method of separating solid matter and liquid
EP0228072A3 (en) Filtration membrane and method of making the same
DE3265302D1 (en) Liquid-liquid extraction by means of microemulsions of substances dissolved in water
JPS57204454A (en) Separating material, separator and separating method of t cell
JPS57149225A (en) Material, apparatus and method for separating t cell
JPS56168814A (en) Barrier for separation of blood
JPS5567309A (en) Liquid-liquid or solid-liquid separating method and separator
GB1377595A (en) Method of treating wastewater
JPS55119457A (en) Floatation separation apparatus
JPS56155683A (en) Floating separator
OA06840A (en) Process for the selective separation of uranium and molydene contained in an amino solvent.
JPS5559817A (en) Fluidized bed type filtering method
JPS5730511A (en) Sedimenting separator
JPS57107210A (en) Purification apparatus
JPS5294877A (en) Separation by membrane
JPS5624574A (en) Halogen ion removing column and capillary type electrophoresis apparatus provided therewith
JPS5687409A (en) Purifying apparatus for oil containing waste water
JPS56161814A (en) Solid-liquid separation apparatus for suspension
JPS5716694A (en) Fixing method of cell
Nakano et al. Ecological Studies on Attached Algae in the Nabara Water System: I. Water Quality(in Japanese)
WHITTEN METHOD OF WATER PURIFICATION
JPS5287859A (en) Sedimentation filtering apparatus
JPS5640491A (en) Sewage clarifying system
JPS57135020A (en) Apparatus for washing, separating and dehydrating screen residue
JPS5282848A (en) Method of pressurized floating separation