JPS57197034A - Preparation of immobilized nucleic acid - Google Patents

Preparation of immobilized nucleic acid

Info

Publication number
JPS57197034A
JPS57197034A JP56081709A JP8170981A JPS57197034A JP S57197034 A JPS57197034 A JP S57197034A JP 56081709 A JP56081709 A JP 56081709A JP 8170981 A JP8170981 A JP 8170981A JP S57197034 A JPS57197034 A JP S57197034A
Authority
JP
Japan
Prior art keywords
nucleic acid
insolubilize
preparation
irradiated
immobilized nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP56081709A
Other languages
Japanese (ja)
Other versions
JPS6128375B2 (en
Inventor
Toshihiro Hirotsu
Hideoki Ogawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institute of Advanced Industrial Science and Technology AIST
Original Assignee
Agency of Industrial Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency of Industrial Science and Technology filed Critical Agency of Industrial Science and Technology
Priority to JP56081709A priority Critical patent/JPS57197034A/en
Publication of JPS57197034A publication Critical patent/JPS57197034A/en
Publication of JPS6128375B2 publication Critical patent/JPS6128375B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

PURPOSE: To immobilize and insolubilize nucleic acid, by a method wherein nucleic acid and a water-soluble high-molecular base material are mixed and irradiated with glow discharge plasma to insolubilize the surface layer part thereof by crosslinked gel formation.
CONSTITUTION: Nucleic acid is mixed with a water-soluble high molecular material as a matrix such as polyvinyl alcohol or gelatine and the mixture is irradiated with glow discharge plasma to insolubilize the surface layer part thereof by crosslinked gel formation. By utilizing this process, nucleic acid is confined into the matrix to prepare immobilized nucleic acid which is useful as a filler material of affinity chromatography and an adsorbent material in an antigen-antibody reaction.
COPYRIGHT: (C)1982,JPO&Japio
JP56081709A 1981-05-28 1981-05-28 Preparation of immobilized nucleic acid Granted JPS57197034A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP56081709A JPS57197034A (en) 1981-05-28 1981-05-28 Preparation of immobilized nucleic acid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP56081709A JPS57197034A (en) 1981-05-28 1981-05-28 Preparation of immobilized nucleic acid

Publications (2)

Publication Number Publication Date
JPS57197034A true JPS57197034A (en) 1982-12-03
JPS6128375B2 JPS6128375B2 (en) 1986-06-30

Family

ID=13753911

Family Applications (1)

Application Number Title Priority Date Filing Date
JP56081709A Granted JPS57197034A (en) 1981-05-28 1981-05-28 Preparation of immobilized nucleic acid

Country Status (1)

Country Link
JP (1) JPS57197034A (en)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001033227A1 (en) * 1999-11-04 2001-05-10 Center For Advanced Science And Technology Incubation, Ltd. Method for immobilizing material
WO2004046714A1 (en) * 2002-11-19 2004-06-03 Daicel Chemical Industries, Ltd. Separating agent for optical isomer
WO2004091753A1 (en) * 2003-04-16 2004-10-28 Nissei Bio Co., Ltd. Method of removing harmful substances and filter for removing harmful substances
JP2006223241A (en) * 2005-02-21 2006-08-31 National Printing Bureau Dna-containing material
WO2007008755A2 (en) * 2005-07-08 2007-01-18 The Board Of Regents, The University Of Texas System Surface functionalization of polymeric materials
JP2007277447A (en) * 2006-04-10 2007-10-25 Inoac Corp Nucleotide chain carrier and application thereof
JP2008001763A (en) * 2006-06-21 2008-01-10 Gunma Univ Method for producing gel composition stably containing deoxyribonucleic acid and the resultant gel composition
JP2008030039A (en) * 2007-08-13 2008-02-14 Nissei Bio Kk Method for removing harmful substance and filter for removing harmful substance
JP2008068254A (en) * 2007-08-13 2008-03-27 Nissei Bio Kk Method and filter for removing harmful substance
JP2008272657A (en) * 2007-04-27 2008-11-13 Canon Inc Dna fixing method, dna porous composite and purification system using this composite
CN106854619A (en) * 2017-01-19 2017-06-16 西安交通大学 A kind of crosslinking apparatus based on plasma, application method and application

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001033227A1 (en) * 1999-11-04 2001-05-10 Center For Advanced Science And Technology Incubation, Ltd. Method for immobilizing material
US7407576B2 (en) 2002-11-19 2008-08-05 Daicel Chemical Industries, Ltd. Separating agent for an optical enantiomeric isomer
WO2004046714A1 (en) * 2002-11-19 2004-06-03 Daicel Chemical Industries, Ltd. Separating agent for optical isomer
US8092677B2 (en) 2002-11-19 2012-01-10 Daicel Chemical Industries, Ltd. Separating agent for an enantiomeric isomer
WO2004091753A1 (en) * 2003-04-16 2004-10-28 Nissei Bio Co., Ltd. Method of removing harmful substances and filter for removing harmful substances
JP2006223241A (en) * 2005-02-21 2006-08-31 National Printing Bureau Dna-containing material
WO2007008755A2 (en) * 2005-07-08 2007-01-18 The Board Of Regents, The University Of Texas System Surface functionalization of polymeric materials
WO2007008755A3 (en) * 2005-07-08 2007-04-19 Univ Texas Surface functionalization of polymeric materials
JP2007277447A (en) * 2006-04-10 2007-10-25 Inoac Corp Nucleotide chain carrier and application thereof
JP2008001763A (en) * 2006-06-21 2008-01-10 Gunma Univ Method for producing gel composition stably containing deoxyribonucleic acid and the resultant gel composition
JP2008272657A (en) * 2007-04-27 2008-11-13 Canon Inc Dna fixing method, dna porous composite and purification system using this composite
JP2008068254A (en) * 2007-08-13 2008-03-27 Nissei Bio Kk Method and filter for removing harmful substance
JP2008030039A (en) * 2007-08-13 2008-02-14 Nissei Bio Kk Method for removing harmful substance and filter for removing harmful substance
CN106854619A (en) * 2017-01-19 2017-06-16 西安交通大学 A kind of crosslinking apparatus based on plasma, application method and application
CN106854619B (en) * 2017-01-19 2023-10-20 西安交通大学 Crosslinking device based on plasma, using method and application

Also Published As

Publication number Publication date
JPS6128375B2 (en) 1986-06-30

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