JPS57179661A - Setting method for standard curve in radioimmunoassay - Google Patents

Setting method for standard curve in radioimmunoassay

Info

Publication number
JPS57179661A
JPS57179661A JP6407881A JP6407881A JPS57179661A JP S57179661 A JPS57179661 A JP S57179661A JP 6407881 A JP6407881 A JP 6407881A JP 6407881 A JP6407881 A JP 6407881A JP S57179661 A JPS57179661 A JP S57179661A
Authority
JP
Japan
Prior art keywords
antigen
values
measured
standard curve
coordinates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP6407881A
Other languages
Japanese (ja)
Other versions
JPS6331052B2 (en
Inventor
Masaaki Takeda
Yoshifumi Okabe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hitachi Ltd
Original Assignee
Aloka Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aloka Co Ltd filed Critical Aloka Co Ltd
Priority to JP6407881A priority Critical patent/JPS57179661A/en
Publication of JPS57179661A publication Critical patent/JPS57179661A/en
Publication of JPS6331052B2 publication Critical patent/JPS6331052B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor

Abstract

PURPOSE:To set an accurate standard curve without complicated operations by subjecting the coordinates of the quantity of antigen to log transformation, by estimating values of radioisotope (RI) at times when the quantity of antigen is 0 and infinite, and by subjecting the coordinates of a measured value of RI to logit transformation based on the measured and estimated values. CONSTITUTION:In setting a standard curve at the time when a linked antibody-labelled- antigen substance is measured by utilizing the competitive reaction between antigen in a known quantity and RI-marked antigen in a prescribed quantity so as to analyze the antigen in a sample, the quantity of antigen is made to be x and this is subjected to log transformation. Next, a measured value y of RI corresponding to x is found, and the measured values y of RI at the times when the quantity of antigen is 0 and infinite are supposed from a plurality of known quantities (x) of antigen and the measured values (y) of RI corresponding thereto by using a specific relational expression, and are made to be B0 and N, respectively. Based on these supposed values B0 and N and (y), the measured values (y) of RI are subjected to logit transformation, and the measured values of RI are represented in the standard curve on the coordinates of the respective transformed values by a method of least squares. By using this standard curve, the quantity of antigen in the sample can be determined with little error from the measured values of the RI of the sample.
JP6407881A 1981-04-30 1981-04-30 Setting method for standard curve in radioimmunoassay Granted JPS57179661A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP6407881A JPS57179661A (en) 1981-04-30 1981-04-30 Setting method for standard curve in radioimmunoassay

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP6407881A JPS57179661A (en) 1981-04-30 1981-04-30 Setting method for standard curve in radioimmunoassay

Publications (2)

Publication Number Publication Date
JPS57179661A true JPS57179661A (en) 1982-11-05
JPS6331052B2 JPS6331052B2 (en) 1988-06-22

Family

ID=13247687

Family Applications (1)

Application Number Title Priority Date Filing Date
JP6407881A Granted JPS57179661A (en) 1981-04-30 1981-04-30 Setting method for standard curve in radioimmunoassay

Country Status (1)

Country Link
JP (1) JPS57179661A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0619347B2 (en) * 1983-06-23 1994-03-16 イーキンズ，ロジャー フィリップ Free ligand measurement

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0619347B2 (en) * 1983-06-23 1994-03-16 イーキンズ，ロジャー フィリップ Free ligand measurement

Also Published As

Publication number Publication date
JPS6331052B2 (en) 1988-06-22

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