JPS57165324A - Cultivation of rabies virus - Google Patents

Cultivation of rabies virus

Info

Publication number
JPS57165324A
JPS57165324A JP4975081A JP4975081A JPS57165324A JP S57165324 A JPS57165324 A JP S57165324A JP 4975081 A JP4975081 A JP 4975081A JP 4975081 A JP4975081 A JP 4975081A JP S57165324 A JPS57165324 A JP S57165324A
Authority
JP
Japan
Prior art keywords
culture
virus
cell
cell strain
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP4975081A
Other languages
Japanese (ja)
Other versions
JPS6225351B2 (en
Inventor
Jiro Tachibana
Reiji Isogai
Hatsue Takano
Yuji Fujikawa
Hiroshi Hamada
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KIYOURITSU SHOJI KK
Kyoritsu Shoji KK
Original Assignee
KIYOURITSU SHOJI KK
Kyoritsu Shoji KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by KIYOURITSU SHOJI KK, Kyoritsu Shoji KK filed Critical KIYOURITSU SHOJI KK
Priority to JP4975081A priority Critical patent/JPS57165324A/en
Publication of JPS57165324A publication Critical patent/JPS57165324A/en
Publication of JPS6225351B2 publication Critical patent/JPS6225351B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

PURPOSE: To obtain inexpensively a large amount of the titled virus liquid having high virus value free from side effects, by cultivating a novel flotation culture cell having succeeded from the hepatic or pulmonary passage cell strain of young hamster using a medium having no serum by spinner culture.
CONSTITUTION: The hepatic passage cell strain of young hamster and the pulmonoary passage cell strain of young hamster are cultivated by single layer culture. After the cell is sufficiently propagated, it is transferred to a spinner culture container for flotation culture, and a trypsine solution is added to the cell to carry out spinner culture. These operations are repeated and the cell is acclimated to give a novel flotation culture cell having succeeded from the hepatic passage cell strain and the pulmonary passage cell strain of young hamster. This is cultivated in a medium containing no bovine serum by spinner culture, to give the desired virus solution. The virus solution has higher virus value than a culture solution of rabies virus using caprine brain, and a vaccine for preventing rabies can be prepared safely at low cost since it has no side effects.
COPYRIGHT: (C)1982,JPO&Japio
JP4975081A 1981-04-02 1981-04-02 Cultivation of rabies virus Granted JPS57165324A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP4975081A JPS57165324A (en) 1981-04-02 1981-04-02 Cultivation of rabies virus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP4975081A JPS57165324A (en) 1981-04-02 1981-04-02 Cultivation of rabies virus

Publications (2)

Publication Number Publication Date
JPS57165324A true JPS57165324A (en) 1982-10-12
JPS6225351B2 JPS6225351B2 (en) 1987-06-02

Family

ID=12839851

Family Applications (1)

Application Number Title Priority Date Filing Date
JP4975081A Granted JPS57165324A (en) 1981-04-02 1981-04-02 Cultivation of rabies virus

Country Status (1)

Country Link
JP (1) JPS57165324A (en)

Also Published As

Publication number Publication date
JPS6225351B2 (en) 1987-06-02

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