JPS6456620A - Production of hemorrhagic fever with renal syndrome virus antigen and vaccine and diagnostic agent using said antigen - Google Patents

Production of hemorrhagic fever with renal syndrome virus antigen and vaccine and diagnostic agent using said antigen

Info

Publication number
JPS6456620A
JPS6456620A JP62213410A JP21341087A JPS6456620A JP S6456620 A JPS6456620 A JP S6456620A JP 62213410 A JP62213410 A JP 62213410A JP 21341087 A JP21341087 A JP 21341087A JP S6456620 A JPS6456620 A JP S6456620A
Authority
JP
Japan
Prior art keywords
antigen
virus
mouse
brain
hfrsv
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP62213410A
Other languages
Japanese (ja)
Other versions
JP2681063B2 (en
Inventor
Osamu Tanishita
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HANDAI BISEIBUTSUBIYOU KENKYUKAI
Osaka University NUC
Original Assignee
HANDAI BISEIBUTSUBIYOU KENKYUKAI
Osaka University NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by HANDAI BISEIBUTSUBIYOU KENKYUKAI, Osaka University NUC filed Critical HANDAI BISEIBUTSUBIYOU KENKYUKAI
Priority to JP62213410A priority Critical patent/JP2681063B2/en
Priority to KR1019880010897A priority patent/KR900007262B1/en
Publication of JPS6456620A publication Critical patent/JPS6456620A/en
Application granted granted Critical
Publication of JP2681063B2 publication Critical patent/JP2681063B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses

Abstract

PURPOSE:To obtain a hemorrhagic fever with renal syndrome virus (HFRSV) antigen, having high phylactic effects and wide antigenic spectrum, by cultivating an acclimated strain of a mouse cerebral cell of the HFRS virus in a mouse brain. CONSTITUTION:HFRSV is subcultured in mouse brains for >=10 passages and acclimated in mouse cerebral cells and the resultant acclimated strain is then cultivated in a mouse brain to collect the above-mentioned mouse brain. A solution containing a virus stabilizer (e.g., enzymic inhibitor, such as chelating agent or basic amino acid) is then added to the brain and the brain is crushed and suspended to prepare a cerebral emulsion in 5-30wt.% concentration. The resultant emulsion is subsequently centrifuged to collect a supernatant and a solution of a viral inactivator (e.g., aldehyde) is added to the supernatant to inactivate the virus and purify a virus antigen. Thereby the aimed HFRSV antigen is obtained. An HFRS vaccine and HFRS diagnostic agent are prepared by using the above-mentioned antigen.
JP62213410A 1987-08-26 1987-08-26 Method for producing renal symptomatic hemorrhagic fever virus antigen, vaccine containing the antigen, and diagnostic agent for renal symptomatic hemorrhagic fever Expired - Lifetime JP2681063B2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP62213410A JP2681063B2 (en) 1987-08-26 1987-08-26 Method for producing renal symptomatic hemorrhagic fever virus antigen, vaccine containing the antigen, and diagnostic agent for renal symptomatic hemorrhagic fever
KR1019880010897A KR900007262B1 (en) 1987-08-26 1988-08-26 Production of hemorr

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62213410A JP2681063B2 (en) 1987-08-26 1987-08-26 Method for producing renal symptomatic hemorrhagic fever virus antigen, vaccine containing the antigen, and diagnostic agent for renal symptomatic hemorrhagic fever

Publications (2)

Publication Number Publication Date
JPS6456620A true JPS6456620A (en) 1989-03-03
JP2681063B2 JP2681063B2 (en) 1997-11-19

Family

ID=16638767

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62213410A Expired - Lifetime JP2681063B2 (en) 1987-08-26 1987-08-26 Method for producing renal symptomatic hemorrhagic fever virus antigen, vaccine containing the antigen, and diagnostic agent for renal symptomatic hemorrhagic fever

Country Status (2)

Country Link
JP (1) JP2681063B2 (en)
KR (1) KR900007262B1 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02297064A (en) * 1989-04-26 1990-12-07 Ki Koo Reagent for diagnosing nephrotic hemorrhagic fever
CN1059836C (en) * 1994-09-21 2000-12-27 卫生部长春生物制品研究所 Production of inactivated vaccine for epidemic hemorrhagic fever using primary cells of kidney of golden yellow hamster
CN1112934C (en) * 1999-06-23 2003-07-02 中国疾病预防控制中心病毒病预防控制所 Epidemic hemorrhagic fever passage cell polyvalent purified vaccine
US8463110B2 (en) 1997-12-23 2013-06-11 Intel Corporation Storage of video stream multiplexed with random access playback after variable time delay
JP2013527759A (en) * 2010-05-07 2013-07-04 ドイッチェス・クレープスフォルシュングスツェントルム Modified rodent parvovirus that can grow and propagate through human glioma

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02297064A (en) * 1989-04-26 1990-12-07 Ki Koo Reagent for diagnosing nephrotic hemorrhagic fever
CN1059836C (en) * 1994-09-21 2000-12-27 卫生部长春生物制品研究所 Production of inactivated vaccine for epidemic hemorrhagic fever using primary cells of kidney of golden yellow hamster
US8463110B2 (en) 1997-12-23 2013-06-11 Intel Corporation Storage of video stream multiplexed with random access playback after variable time delay
CN1112934C (en) * 1999-06-23 2003-07-02 中国疾病预防控制中心病毒病预防控制所 Epidemic hemorrhagic fever passage cell polyvalent purified vaccine
JP2013527759A (en) * 2010-05-07 2013-07-04 ドイッチェス・クレープスフォルシュングスツェントルム Modified rodent parvovirus that can grow and propagate through human glioma

Also Published As

Publication number Publication date
KR890003406A (en) 1989-04-14
JP2681063B2 (en) 1997-11-19
KR900007262B1 (en) 1990-10-06

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