JPS6434925A - Method for cultivating bordetella pertussis, pertussis toxoid and mixed vaccine thereof - Google Patents

Method for cultivating bordetella pertussis, pertussis toxoid and mixed vaccine thereof

Info

Publication number
JPS6434925A
JPS6434925A JP63103405A JP10340588A JPS6434925A JP S6434925 A JPS6434925 A JP S6434925A JP 63103405 A JP63103405 A JP 63103405A JP 10340588 A JP10340588 A JP 10340588A JP S6434925 A JPS6434925 A JP S6434925A
Authority
JP
Japan
Prior art keywords
culture medium
pertussis
bordetella pertussis
derivative
added
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP63103405A
Other languages
Japanese (ja)
Other versions
JP2743177B2 (en
Inventor
Masachika Chazono
Iwao Yoshida
Takeo Takanobu
Juichiro Osame
Yoshinori Takaku
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HANDAI BISEIBUTSUBIYOU KENKYUKAI
Osaka University NUC
Original Assignee
HANDAI BISEIBUTSUBIYOU KENKYUKAI
Osaka University NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by HANDAI BISEIBUTSUBIYOU KENKYUKAI, Osaka University NUC filed Critical HANDAI BISEIBUTSUBIYOU KENKYUKAI
Priority to JP63103405A priority Critical patent/JP2743177B2/en
Publication of JPS6434925A publication Critical patent/JPS6434925A/en
Application granted granted Critical
Publication of JP2743177B2 publication Critical patent/JP2743177B2/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

PURPOSE:To obtain a high-purity pertussis toxin (PT) and fibrous hemagglutinin (F-HA) in high yield at an extremely low cost, by cultivating Bordetella pertussis in a culture medium containing cellulose (derivative). CONSTITUTION:Cellulose or a derivative thereof or both are added and blended with a basal culture medium (e.g. Cohen.Wheeler culture medium) used for cultivating Bordetella pertussis so as to provide 0.01-2.0wt.% final concentration and the Bordetella pertussis is cultivated in the resultant modified culture medium at 30-37 deg.C for 20-80hr. The obtained culture is subsequently separated into a cultivation supernatant and microbial cells. The above-mentioned cultivation supernatant is then purified to recover PT and F-HA. Formalin is subsequently added and mixed to detoxify the purified PT and F-HA fraction and formalin is removed by dialysis. The resultant dialyzate is diluted with a phosphoric acid buffer solution, etc., to provide a vaccine concentrate, which is then used to prepare a mixed vaccine. Gelatin (derivative) in an amount of 0.1-5.0wt.% as a stabilizer is added to remarkably improve heat and cold resistance.
JP63103405A 1987-04-24 1988-04-25 Method of culturing pertussis, pertussis toxoid and combination vaccine thereof Expired - Fee Related JP2743177B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP63103405A JP2743177B2 (en) 1987-04-24 1988-04-25 Method of culturing pertussis, pertussis toxoid and combination vaccine thereof

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP10236087 1987-04-24
JP62-102360 1987-04-24
JP63103405A JP2743177B2 (en) 1987-04-24 1988-04-25 Method of culturing pertussis, pertussis toxoid and combination vaccine thereof

Publications (2)

Publication Number Publication Date
JPS6434925A true JPS6434925A (en) 1989-02-06
JP2743177B2 JP2743177B2 (en) 1998-04-22

Family

ID=26443067

Family Applications (1)

Application Number Title Priority Date Filing Date
JP63103405A Expired - Fee Related JP2743177B2 (en) 1987-04-24 1988-04-25 Method of culturing pertussis, pertussis toxoid and combination vaccine thereof

Country Status (1)

Country Link
JP (1) JP2743177B2 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002005846A1 (en) * 2000-07-05 2002-01-24 Green Cross Vaccine Co., Ltd. Quadrivalent combination vaccine including diphtheria toxoid, tetanus toxoid, whole cell pertussis and hepatitis b surface antigen, and preparation method thereof
JP2002523470A (en) * 1998-09-01 2002-07-30 エラン コーポレーシヨン ピーエルシー Oral vaccine composition
JP2011242408A (en) * 2011-08-04 2011-12-01 Collagen Gijutsu Kenshukai Kk Method of measuring anti-gelatin antibody
JP2015529462A (en) * 2012-08-30 2015-10-08 メリアル インコーポレイテッド High pressure devices and methods for making inactivated vaccines and for refolding / solubilizing recombinant proteins
CN111855827A (en) * 2019-04-24 2020-10-30 岛津企业管理（中国）有限公司 Method for determining polysaccharide protein binding site binding rate in polysaccharide protein binding vaccine

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62253377A (en) * 1986-03-27 1987-11-05 パスツール　メリウー　セロム　エ　ヴアクサン Medium and method for culture of bordetella bacteria

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62253377A (en) * 1986-03-27 1987-11-05 パスツール　メリウー　セロム　エ　ヴアクサン Medium and method for culture of bordetella bacteria

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002523470A (en) * 1998-09-01 2002-07-30 エラン コーポレーシヨン ピーエルシー Oral vaccine composition
WO2002005846A1 (en) * 2000-07-05 2002-01-24 Green Cross Vaccine Co., Ltd. Quadrivalent combination vaccine including diphtheria toxoid, tetanus toxoid, whole cell pertussis and hepatitis b surface antigen, and preparation method thereof
JP2011242408A (en) * 2011-08-04 2011-12-01 Collagen Gijutsu Kenshukai Kk Method of measuring anti-gelatin antibody
JP2015529462A (en) * 2012-08-30 2015-10-08 メリアル インコーポレイテッド High pressure devices and methods for making inactivated vaccines and for refolding / solubilizing recombinant proteins
CN111855827A (en) * 2019-04-24 2020-10-30 岛津企业管理（中国）有限公司 Method for determining polysaccharide protein binding site binding rate in polysaccharide protein binding vaccine
CN111855827B (en) * 2019-04-24 2022-09-16 岛津企业管理（中国）有限公司 Method for determining polysaccharide protein binding site binding rate in polysaccharide protein binding vaccine

Also Published As

Publication number Publication date
JP2743177B2 (en) 1998-04-22

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Legal Events

Date Code Title Description
LAPS Cancellation because of no payment of annual fees