JPS57104864A - Measuring reagent of whole bilirubin or direct bilirubin - Google Patents

Measuring reagent of whole bilirubin or direct bilirubin

Info

Publication number
JPS57104864A
JPS57104864A JP18227880A JP18227880A JPS57104864A JP S57104864 A JPS57104864 A JP S57104864A JP 18227880 A JP18227880 A JP 18227880A JP 18227880 A JP18227880 A JP 18227880A JP S57104864 A JPS57104864 A JP S57104864A
Authority
JP
Japan
Prior art keywords
bilirubin
stabilized
group
measuring reagent
reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP18227880A
Other languages
Japanese (ja)
Other versions
JPS6322263B2 (en
Inventor
Noboru Mitsuhida
Yoshitaka Nakagiri
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toyobo Co Ltd
Original Assignee
Toyobo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toyobo Co Ltd filed Critical Toyobo Co Ltd
Priority to JP18227880A priority Critical patent/JPS57104864A/en
Publication of JPS57104864A publication Critical patent/JPS57104864A/en
Publication of JPS6322263B2 publication Critical patent/JPS6322263B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/728—Bilirubin; including biliverdin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE:To provide bilirubin measuring reagent with reaction rate and chromophoric property being significantly raised, by a method wherein the reagent contains stabilized diazonium salt, hydrochloric acid, aniline derivative in nucleus- substitution by electrophilic group and accelerating agent if required. CONSTITUTION:Direct bilirubin measuring reagent is constituted by hydrochloric acid of 0.01-0.1 N, stabilized diazonium salt (e.g. p-sulfo benzene diazonium chloride stabilized by 1.5-naphthalene disulfonic acid) of 0.05-1.0 millimole/l, and aniline derivative of 0.1-20 millimole/l in nucleus-substitution by electrophilic group (e.g. nitro group, alkyl group, aryl sulfonyl group). Stabilized diazonium in this constituent acts to suppress influence of bilirubin indirectly and raise the reaction rate and chromophoric property significantly, thereby the measuring is effected accurately and rapidly.
JP18227880A 1980-12-22 1980-12-22 Measuring reagent of whole bilirubin or direct bilirubin Granted JPS57104864A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP18227880A JPS57104864A (en) 1980-12-22 1980-12-22 Measuring reagent of whole bilirubin or direct bilirubin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP18227880A JPS57104864A (en) 1980-12-22 1980-12-22 Measuring reagent of whole bilirubin or direct bilirubin

Publications (2)

Publication Number Publication Date
JPS57104864A true JPS57104864A (en) 1982-06-30
JPS6322263B2 JPS6322263B2 (en) 1988-05-11

Family

ID=16115467

Family Applications (1)

Application Number Title Priority Date Filing Date
JP18227880A Granted JPS57104864A (en) 1980-12-22 1980-12-22 Measuring reagent of whole bilirubin or direct bilirubin

Country Status (1)

Country Link
JP (1) JPS57104864A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58180952A (en) * 1982-04-17 1983-10-22 Sanko Junyaku Kk Assay of direct-acting bilirubin in serum

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58180952A (en) * 1982-04-17 1983-10-22 Sanko Junyaku Kk Assay of direct-acting bilirubin in serum
JPH0339266B2 (en) * 1982-04-17 1991-06-13 Sanko Junyaku Kk

Also Published As

Publication number Publication date
JPS6322263B2 (en) 1988-05-11

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