JPS57104864A - Measuring reagent of whole bilirubin or direct bilirubin - Google Patents
Measuring reagent of whole bilirubin or direct bilirubinInfo
- Publication number
- JPS57104864A JPS57104864A JP18227880A JP18227880A JPS57104864A JP S57104864 A JPS57104864 A JP S57104864A JP 18227880 A JP18227880 A JP 18227880A JP 18227880 A JP18227880 A JP 18227880A JP S57104864 A JPS57104864 A JP S57104864A
- Authority
- JP
- Japan
- Prior art keywords
- bilirubin
- stabilized
- group
- measuring reagent
- reagent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/728—Bilirubin; including biliverdin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
PURPOSE:To provide bilirubin measuring reagent with reaction rate and chromophoric property being significantly raised, by a method wherein the reagent contains stabilized diazonium salt, hydrochloric acid, aniline derivative in nucleus- substitution by electrophilic group and accelerating agent if required. CONSTITUTION:Direct bilirubin measuring reagent is constituted by hydrochloric acid of 0.01-0.1 N, stabilized diazonium salt (e.g. p-sulfo benzene diazonium chloride stabilized by 1.5-naphthalene disulfonic acid) of 0.05-1.0 millimole/l, and aniline derivative of 0.1-20 millimole/l in nucleus-substitution by electrophilic group (e.g. nitro group, alkyl group, aryl sulfonyl group). Stabilized diazonium in this constituent acts to suppress influence of bilirubin indirectly and raise the reaction rate and chromophoric property significantly, thereby the measuring is effected accurately and rapidly.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP18227880A JPS57104864A (en) | 1980-12-22 | 1980-12-22 | Measuring reagent of whole bilirubin or direct bilirubin |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP18227880A JPS57104864A (en) | 1980-12-22 | 1980-12-22 | Measuring reagent of whole bilirubin or direct bilirubin |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS57104864A true JPS57104864A (en) | 1982-06-30 |
JPS6322263B2 JPS6322263B2 (en) | 1988-05-11 |
Family
ID=16115467
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP18227880A Granted JPS57104864A (en) | 1980-12-22 | 1980-12-22 | Measuring reagent of whole bilirubin or direct bilirubin |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS57104864A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS58180952A (en) * | 1982-04-17 | 1983-10-22 | Sanko Junyaku Kk | Assay of direct-acting bilirubin in serum |
-
1980
- 1980-12-22 JP JP18227880A patent/JPS57104864A/en active Granted
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS58180952A (en) * | 1982-04-17 | 1983-10-22 | Sanko Junyaku Kk | Assay of direct-acting bilirubin in serum |
JPH0339266B2 (en) * | 1982-04-17 | 1991-06-13 | Sanko Junyaku Kk |
Also Published As
Publication number | Publication date |
---|---|
JPS6322263B2 (en) | 1988-05-11 |
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