JPS5688794A - Immobilization of enzyme - Google Patents
Immobilization of enzymeInfo
- Publication number
- JPS5688794A JPS5688794A JP16586779A JP16586779A JPS5688794A JP S5688794 A JPS5688794 A JP S5688794A JP 16586779 A JP16586779 A JP 16586779A JP 16586779 A JP16586779 A JP 16586779A JP S5688794 A JPS5688794 A JP S5688794A
- Authority
- JP
- Japan
- Prior art keywords
- enzyme
- solution
- immobilization
- resin
- reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
PURPOSE: To make the control of the procedure degree of the immobilization reaction of enzyme easy, by covering previously the surface of a carrier for immobilization of enzyme with an enzyme solution wherein a resin as well as the enzyme are dissolved in an organic solvent, drying the surface, followed by feeding an immobilizing reagent from a gas phase to it.
CONSTITUTION: 100mg/ml urease aqueous solution as an enzyme solution and 10g/ dl solution of polymethyl methacryrate in acetone as a resin solution are prepared. 1pts.wt. enzyme solution is blended with 9pts.wt. resin solution to give a mixed solution, which is applied to the sensitive part of the glass electrode 3 by dipping, etc. to cover the part with it. The covered part is dried to remove the solvent, and the immobilization reaction of the enzyme is carried out in the vapor of glutaraldehyde at 25°C for 60W90min, to give the enzyme immobilization film 4 having sufficient adhesiveness and mechanical strength. After the reaction, the film is washed in a buffer solution of phosphoric acid. 2-Oxyadipaldehyde, acrolein, etc. besides, glutaraldehyde is used as the immobilizing reagent.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP16586779A JPS5688794A (en) | 1979-12-19 | 1979-12-19 | Immobilization of enzyme |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP16586779A JPS5688794A (en) | 1979-12-19 | 1979-12-19 | Immobilization of enzyme |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5688794A true JPS5688794A (en) | 1981-07-18 |
JPS6326992B2 JPS6326992B2 (en) | 1988-06-01 |
Family
ID=15820484
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP16586779A Granted JPS5688794A (en) | 1979-12-19 | 1979-12-19 | Immobilization of enzyme |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5688794A (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS61170645A (en) * | 1985-01-24 | 1986-08-01 | Agency Of Ind Science & Technol | Ion selective electrode consisting of chemically modified glass film |
JPS63111454A (en) * | 1986-10-29 | 1988-05-16 | Nec Corp | Production of immobilized enzyme film |
US4894226A (en) * | 1986-11-14 | 1990-01-16 | Cetus Corporation | Solubilization of proteins for pharmaceutical compositions using polyproline conjugation |
CN105699459A (en) * | 2016-03-12 | 2016-06-22 | 云南圣清环境监测科技有限公司 | Preparation method of composite biosensor for water-quality toxicity prewarning during sewage treatment |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5715878A (en) * | 1980-06-30 | 1982-01-27 | Iseki Agricult Mach | Feeder for cereal grain in coarse selector |
-
1979
- 1979-12-19 JP JP16586779A patent/JPS5688794A/en active Granted
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5715878A (en) * | 1980-06-30 | 1982-01-27 | Iseki Agricult Mach | Feeder for cereal grain in coarse selector |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS61170645A (en) * | 1985-01-24 | 1986-08-01 | Agency Of Ind Science & Technol | Ion selective electrode consisting of chemically modified glass film |
JPS63111454A (en) * | 1986-10-29 | 1988-05-16 | Nec Corp | Production of immobilized enzyme film |
US4894226A (en) * | 1986-11-14 | 1990-01-16 | Cetus Corporation | Solubilization of proteins for pharmaceutical compositions using polyproline conjugation |
CN105699459A (en) * | 2016-03-12 | 2016-06-22 | 云南圣清环境监测科技有限公司 | Preparation method of composite biosensor for water-quality toxicity prewarning during sewage treatment |
Also Published As
Publication number | Publication date |
---|---|
JPS6326992B2 (en) | 1988-06-01 |
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