JPS5657937A - Measuring device of enzyme activity - Google Patents

Measuring device of enzyme activity

Info

Publication number
JPS5657937A
JPS5657937A JP13345679A JP13345679A JPS5657937A JP S5657937 A JPS5657937 A JP S5657937A JP 13345679 A JP13345679 A JP 13345679A JP 13345679 A JP13345679 A JP 13345679A JP S5657937 A JPS5657937 A JP S5657937A
Authority
JP
Japan
Prior art keywords
change
enzyme
measurement
enzyme activity
reaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP13345679A
Other languages
Japanese (ja)
Other versions
JPH0262822B2 (en
Inventor
Kazuo Furusawa
Yoshio Tsunasawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shimadzu Corp
Original Assignee
Shimadzu Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shimadzu Corp filed Critical Shimadzu Corp
Priority to JP13345679A priority Critical patent/JPS5657937A/en
Publication of JPS5657937A publication Critical patent/JPS5657937A/en
Publication of JPH0262822B2 publication Critical patent/JPH0262822B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/272—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration for following a reaction, e.g. for determining photometrically a reaction rate (photometric cinetic analysis)

Abstract

PURPOSE:To measure the value of the enzyme activity, by measuring the change of the light absorbance which is caused by the change of concentration of the reaction product or the substrate of the early stage of the enzyme reaction by the enzyme in the blood serum and then referring to the velocity of the change of light absorbance. CONSTITUTION:The enzyme in the blood serum and the reactive substrate are introduced to the reaction part 2 to secure a reaction of them, and the light absorbance at that time is detected by the lamp and the spectroscope. the measurement start time and the time of measurement are stored in the microprocessor system 6 for an automatic measurement of the change of the absorbance. The system 6 includes the CPU8, memories 9 and 10, interface 11, keyboard and others, and then can set freely the conditions of measurement by the input given from the keyboard 7. Then the measured value is processed by the CPU to obtain the value of the enzyme activity.
JP13345679A 1979-10-15 1979-10-15 Measuring device of enzyme activity Granted JPS5657937A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP13345679A JPS5657937A (en) 1979-10-15 1979-10-15 Measuring device of enzyme activity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP13345679A JPS5657937A (en) 1979-10-15 1979-10-15 Measuring device of enzyme activity

Publications (2)

Publication Number Publication Date
JPS5657937A true JPS5657937A (en) 1981-05-20
JPH0262822B2 JPH0262822B2 (en) 1990-12-26

Family

ID=15105198

Family Applications (1)

Application Number Title Priority Date Filing Date
JP13345679A Granted JPS5657937A (en) 1979-10-15 1979-10-15 Measuring device of enzyme activity

Country Status (1)

Country Link
JP (1) JPS5657937A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59116549A (en) * 1982-11-30 1984-07-05 日本テクトロン株式会社 Method and apparatus for monitoring analysis data for automatic analyzer
JPS6181774A (en) * 1984-09-29 1986-04-25 Shimadzu Corp Apparatus for determination of enzymatic activity
JPS61128130A (en) * 1984-11-27 1986-06-16 Shimadzu Corp Spectroscopical measuring apparatus of change with time
CN101936909A (en) * 2010-08-31 2011-01-05 天津工业大学 Enzyme activity testing method for micro-biological degradation of benzene ring

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4918395A (en) * 1972-04-17 1974-02-18

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4918395A (en) * 1972-04-17 1974-02-18

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59116549A (en) * 1982-11-30 1984-07-05 日本テクトロン株式会社 Method and apparatus for monitoring analysis data for automatic analyzer
JPH0534626B2 (en) * 1982-11-30 1993-05-24 Orinpasu Kogaku Kogyo Kk
JPS6181774A (en) * 1984-09-29 1986-04-25 Shimadzu Corp Apparatus for determination of enzymatic activity
JPS61128130A (en) * 1984-11-27 1986-06-16 Shimadzu Corp Spectroscopical measuring apparatus of change with time
CN101936909A (en) * 2010-08-31 2011-01-05 天津工业大学 Enzyme activity testing method for micro-biological degradation of benzene ring

Also Published As

Publication number Publication date
JPH0262822B2 (en) 1990-12-26

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