JPS57178159A - Chemical reagent for detection of amicacin and its determination - Google Patents
Chemical reagent for detection of amicacin and its determinationInfo
- Publication number
- JPS57178159A JPS57178159A JP6239481A JP6239481A JPS57178159A JP S57178159 A JPS57178159 A JP S57178159A JP 6239481 A JP6239481 A JP 6239481A JP 6239481 A JP6239481 A JP 6239481A JP S57178159 A JPS57178159 A JP S57178159A
- Authority
- JP
- Japan
- Prior art keywords
- amicacin
- blood
- antibody
- labelled
- glucose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
- G01N33/9446—Antibacterials
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Pharmacology & Pharmacy (AREA)
- Food Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE:To measure the concentration of amicacin in blood quickly and to prescribe a medicine safely, by combining amicacin labelled with glucose-6- phosphoric acid dehydrogenase with anti-amicacin antibody conflictingly with amicacin and measuring enzyme activity after reaction. CONSTITUTION:Labelled amicacin is obtained by reacting amicacin with glucose- 6-phosphoric acid d hydrogenase for safety medication of remedy amicacin for bacteria infections symptoms. On one hand, amicacin is immunized to a rabbit and an antibody is obtained from its blood and then, a calibration curve based on a competitive reaction of the mixture of the marking amicacin and already known quantity of amicacin for the antibody, is found preliminarily. The concentration of amicacin in blood of a patient is determined by said calibration curve. In the case where the specimen is blood plasma, citric acid or EDTA is used for an anticoagulant. Measurement is obstructed by using heparin. Thus, amicacin is determined in high sensitivity and good reproducibility.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6239481A JPS57178159A (en) | 1981-04-27 | 1981-04-27 | Chemical reagent for detection of amicacin and its determination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6239481A JPS57178159A (en) | 1981-04-27 | 1981-04-27 | Chemical reagent for detection of amicacin and its determination |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS57178159A true JPS57178159A (en) | 1982-11-02 |
Family
ID=13198868
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP6239481A Pending JPS57178159A (en) | 1981-04-27 | 1981-04-27 | Chemical reagent for detection of amicacin and its determination |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS57178159A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111537452A (en) * | 2019-01-09 | 2020-08-14 | 北京九强生物技术股份有限公司 | 6-glucose phosphate dehydrogenase mutant and application thereof in preparation of amikacin detection reagent |
| CN114839295A (en) * | 2022-05-07 | 2022-08-02 | 杭州度安医学检验实验室有限公司 | LC-MS-based method for detecting amikacin in human serum |
-
1981
- 1981-04-27 JP JP6239481A patent/JPS57178159A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111537452A (en) * | 2019-01-09 | 2020-08-14 | 北京九强生物技术股份有限公司 | 6-glucose phosphate dehydrogenase mutant and application thereof in preparation of amikacin detection reagent |
| CN111537452B (en) * | 2019-01-09 | 2023-07-04 | 北京九强生物技术股份有限公司 | Glucose 6-phosphate dehydrogenase mutant and application thereof in preparation of amikacin detection reagent |
| CN114839295A (en) * | 2022-05-07 | 2022-08-02 | 杭州度安医学检验实验室有限公司 | LC-MS-based method for detecting amikacin in human serum |
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