JPS5630647A - Method of measuring pituitary hormone - Google Patents
Method of measuring pituitary hormoneInfo
- Publication number
- JPS5630647A JPS5630647A JP10616279A JP10616279A JPS5630647A JP S5630647 A JPS5630647 A JP S5630647A JP 10616279 A JP10616279 A JP 10616279A JP 10616279 A JP10616279 A JP 10616279A JP S5630647 A JPS5630647 A JP S5630647A
- Authority
- JP
- Japan
- Prior art keywords
- hormone
- pituitary hormone
- labeled
- galactosidase
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To heighten sensitivity and accuracy, by using β-D-galactosidase as enzyme for a label, causing pituitary hormone and an antibody to react with each other and thereafter bringing the resultant into contact with pituitary hormone labeled with the enzyme.
CONSTITUTION: A liquid, which contains pituitary hormone to be measured, and a prescribed quantity of a primary antibody corresponding to hormone are caused to react with each other to produce a combined substance of antigen and antibody. A measuring reagent containing pituitary hormone labeled with β-D-galactosidase is added to the combined substance so that some of the pituitary hormone labeled with the β-D-galactosidase is linked to the combined substance. The ratio of the labeled hormone linked to the combined substance to the labeled hormone not linked to the substance and the activity of the β-D-galactosidase, which is enzyme for the label, are measured. The quantity of the pituitary hormone is calculated from the activity of the enzyme.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10616279A JPS5630647A (en) | 1979-08-21 | 1979-08-21 | Method of measuring pituitary hormone |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10616279A JPS5630647A (en) | 1979-08-21 | 1979-08-21 | Method of measuring pituitary hormone |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5630647A true JPS5630647A (en) | 1981-03-27 |
Family
ID=14426580
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP10616279A Pending JPS5630647A (en) | 1979-08-21 | 1979-08-21 | Method of measuring pituitary hormone |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5630647A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2016140063A1 (en) * | 2015-03-04 | 2016-09-09 | 栄研化学株式会社 | Method for measuring oxytocin with high sensitivity |
-
1979
- 1979-08-21 JP JP10616279A patent/JPS5630647A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2016140063A1 (en) * | 2015-03-04 | 2016-09-09 | 栄研化学株式会社 | Method for measuring oxytocin with high sensitivity |
JPWO2016140063A1 (en) * | 2015-03-04 | 2017-12-14 | 栄研化学株式会社 | High-sensitivity measurement method for oxytocin |
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