JPS55135752A - Determination method of immunogloblin e in human blood by enzyme immunity measuring method - Google Patents

Determination method of immunogloblin e in human blood by enzyme immunity measuring method

Info

Publication number
JPS55135752A
JPS55135752A JP4386379A JP4386379A JPS55135752A JP S55135752 A JPS55135752 A JP S55135752A JP 4386379 A JP4386379 A JP 4386379A JP 4386379 A JP4386379 A JP 4386379A JP S55135752 A JPS55135752 A JP S55135752A
Authority
JP
Japan
Prior art keywords
immunogloblin
human blood
enzyme
flagment
ige
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP4386379A
Other languages
Japanese (ja)
Inventor
Minoru Imokawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
IGAKU SEIBUTSUGAKU KENKYUSHO K
Medical and Biological Laboratories Co Ltd
Original Assignee
IGAKU SEIBUTSUGAKU KENKYUSHO K
Medical and Biological Laboratories Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by IGAKU SEIBUTSUGAKU KENKYUSHO K, Medical and Biological Laboratories Co Ltd filed Critical IGAKU SEIBUTSUGAKU KENKYUSHO K
Priority to JP4386379A priority Critical patent/JPS55135752A/en
Publication of JPS55135752A publication Critical patent/JPS55135752A/en
Pending legal-status Critical Current

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: To avoid the effect of serum protein, even if a number of serum is in coexistence in IgE, by bonding IgE (antigen) in human blood to be measured with the antibody not soluble.
CONSTITUTION: Anti human-immunogloblin E originated from goat as the antibody marking enzyme is partially refined by using diethylamine ethylecullulose, and reducing operation is further made with pepsin as pepF(ab)2 flagment, as Fab flagment, μ-D-galactosidase originated from colon bacillus is used. The activity of enzyme is obtained by measuring the amount of 0-nitrophenol produced, through the use of 2-nitrophenyl-β-D-galactopyranoside as substrate.
COPYRIGHT: (C)1980,JPO&Japio
JP4386379A 1979-04-09 1979-04-09 Determination method of immunogloblin e in human blood by enzyme immunity measuring method Pending JPS55135752A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP4386379A JPS55135752A (en) 1979-04-09 1979-04-09 Determination method of immunogloblin e in human blood by enzyme immunity measuring method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP4386379A JPS55135752A (en) 1979-04-09 1979-04-09 Determination method of immunogloblin e in human blood by enzyme immunity measuring method

Publications (1)

Publication Number Publication Date
JPS55135752A true JPS55135752A (en) 1980-10-22

Family

ID=12675529

Family Applications (1)

Application Number Title Priority Date Filing Date
JP4386379A Pending JPS55135752A (en) 1979-04-09 1979-04-09 Determination method of immunogloblin e in human blood by enzyme immunity measuring method

Country Status (1)

Country Link
JP (1) JPS55135752A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57208459A (en) * 1981-06-19 1982-12-21 Eisai Co Ltd Measuring method using enzyme-labelled antibody and reagent
JPS59193356A (en) * 1983-03-17 1984-11-01 バイオウィッテッカー・インコーポレーテッド Fluorescent luminous intensity analysis for total ige concentration and reagent therefor
JPS61140865A (en) * 1984-12-14 1986-06-27 Toyo Jozo Co Ltd Measuring method of antinephritis strain associated protein antibody
JPH05133953A (en) * 1991-09-19 1993-05-28 Tokyo Hokenkai Method of detecting biotin bound immunoglobulin in serum

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57208459A (en) * 1981-06-19 1982-12-21 Eisai Co Ltd Measuring method using enzyme-labelled antibody and reagent
JPS59193356A (en) * 1983-03-17 1984-11-01 バイオウィッテッカー・インコーポレーテッド Fluorescent luminous intensity analysis for total ige concentration and reagent therefor
JPS61140865A (en) * 1984-12-14 1986-06-27 Toyo Jozo Co Ltd Measuring method of antinephritis strain associated protein antibody
JPH05133953A (en) * 1991-09-19 1993-05-28 Tokyo Hokenkai Method of detecting biotin bound immunoglobulin in serum

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