JPS55135752A - Determination method of immunogloblin e in human blood by enzyme immunity measuring method - Google Patents
Determination method of immunogloblin e in human blood by enzyme immunity measuring methodInfo
- Publication number
- JPS55135752A JPS55135752A JP4386379A JP4386379A JPS55135752A JP S55135752 A JPS55135752 A JP S55135752A JP 4386379 A JP4386379 A JP 4386379A JP 4386379 A JP4386379 A JP 4386379A JP S55135752 A JPS55135752 A JP S55135752A
- Authority
- JP
- Japan
- Prior art keywords
- immunogloblin
- human blood
- enzyme
- flagment
- ige
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To avoid the effect of serum protein, even if a number of serum is in coexistence in IgE, by bonding IgE (antigen) in human blood to be measured with the antibody not soluble.
CONSTITUTION: Anti human-immunogloblin E originated from goat as the antibody marking enzyme is partially refined by using diethylamine ethylecullulose, and reducing operation is further made with pepsin as pepF(ab)2 flagment, as Fab flagment, μ-D-galactosidase originated from colon bacillus is used. The activity of enzyme is obtained by measuring the amount of 0-nitrophenol produced, through the use of 2-nitrophenyl-β-D-galactopyranoside as substrate.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4386379A JPS55135752A (en) | 1979-04-09 | 1979-04-09 | Determination method of immunogloblin e in human blood by enzyme immunity measuring method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4386379A JPS55135752A (en) | 1979-04-09 | 1979-04-09 | Determination method of immunogloblin e in human blood by enzyme immunity measuring method |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS55135752A true JPS55135752A (en) | 1980-10-22 |
Family
ID=12675529
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP4386379A Pending JPS55135752A (en) | 1979-04-09 | 1979-04-09 | Determination method of immunogloblin e in human blood by enzyme immunity measuring method |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS55135752A (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS57208459A (en) * | 1981-06-19 | 1982-12-21 | Eisai Co Ltd | Measuring method using enzyme-labelled antibody and reagent |
JPS59193356A (en) * | 1983-03-17 | 1984-11-01 | バイオウィッテッカー・インコーポレーテッド | Fluorescent luminous intensity analysis for total ige concentration and reagent therefor |
JPS61140865A (en) * | 1984-12-14 | 1986-06-27 | Toyo Jozo Co Ltd | Measuring method of antinephritis strain associated protein antibody |
JPH05133953A (en) * | 1991-09-19 | 1993-05-28 | Tokyo Hokenkai | Method of detecting biotin bound immunoglobulin in serum |
-
1979
- 1979-04-09 JP JP4386379A patent/JPS55135752A/en active Pending
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS57208459A (en) * | 1981-06-19 | 1982-12-21 | Eisai Co Ltd | Measuring method using enzyme-labelled antibody and reagent |
JPS59193356A (en) * | 1983-03-17 | 1984-11-01 | バイオウィッテッカー・インコーポレーテッド | Fluorescent luminous intensity analysis for total ige concentration and reagent therefor |
JPS61140865A (en) * | 1984-12-14 | 1986-06-27 | Toyo Jozo Co Ltd | Measuring method of antinephritis strain associated protein antibody |
JPH05133953A (en) * | 1991-09-19 | 1993-05-28 | Tokyo Hokenkai | Method of detecting biotin bound immunoglobulin in serum |
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