JPS56164787A - Production of heat-resistant pyruvate kinase - Google Patents

Production of heat-resistant pyruvate kinase

Info

Publication number
JPS56164787A
JPS56164787A JP6801880A JP6801880A JPS56164787A JP S56164787 A JPS56164787 A JP S56164787A JP 6801880 A JP6801880 A JP 6801880A JP 6801880 A JP6801880 A JP 6801880A JP S56164787 A JPS56164787 A JP S56164787A
Authority
JP
Japan
Prior art keywords
pyruvate kinase
strain
heat
cultured
resistant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP6801880A
Other languages
Japanese (ja)
Inventor
Hiroshi Nakajima
Tadao Suzuki
Toyohiko Suga
Kazuhiko Nagata
Masao Kageyama
Kenzo Motosugi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unitika Ltd
Original Assignee
Unitika Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unitika Ltd filed Critical Unitika Ltd
Priority to JP6801880A priority Critical patent/JPS56164787A/en
Priority to DK496080A priority patent/DK496080A/en
Priority to US06/209,097 priority patent/US4331762A/en
Priority to DE8080107281T priority patent/DE3070039D1/en
Priority to CA000365249A priority patent/CA1156573A/en
Priority to EP80107281A priority patent/EP0029976B1/en
Publication of JPS56164787A publication Critical patent/JPS56164787A/en
Pending legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: A specific strain in Bacillus stearothermophilus is cultured and pyruvate kinase is collected from the culture mixture, thus permitting the practical production of heat-resistant pyruvate kinase in a large scale.
CONSTITUTION: Bacillus stearothermophilus UK788 strain is cultured and heat-resistant pyruvate kinase is collected from the resultant culure mixture. The culture medium for the UK788 strain is preferably liquid and the strain is cultured at 20W 80°C batchwise or continuously. In the batchwise operation, the cultivation is preferably continued till the late stage in the logarithmic growth. In the continuous cultivation, the chemostat-type continuous cultivation is conducted near the maximum specific growth rate of the UK788 strain, thus producing cell bodies containing the heat-resistant pyruvate kinase in a high concentration.
COPYRIGHT: (C)1981,JPO&Japio
JP6801880A 1979-11-22 1980-05-21 Production of heat-resistant pyruvate kinase Pending JPS56164787A (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP6801880A JPS56164787A (en) 1980-05-21 1980-05-21 Production of heat-resistant pyruvate kinase
DK496080A DK496080A (en) 1979-11-22 1980-11-20 MICROORGANISM CULTURE AND ITS APPLICATION FOR MANUFACTURING INTRACELLULAR PRODUCTS
US06/209,097 US4331762A (en) 1980-04-18 1980-11-21 Bacillus stearothermophilus strain UK 788 and process for producing a useful enzyme
DE8080107281T DE3070039D1 (en) 1979-11-22 1980-11-21 A biologically pure culture of strain ifo 14093 and a process for producing an intracellular component herefrom
CA000365249A CA1156573A (en) 1980-04-18 1980-11-21 Strain uk 788 and process for producing a useful enzyme
EP80107281A EP0029976B1 (en) 1979-11-22 1980-11-21 A biologically pure culture of strain ifo 14093 and a process for producing an intracellular component herefrom

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP6801880A JPS56164787A (en) 1980-05-21 1980-05-21 Production of heat-resistant pyruvate kinase

Publications (1)

Publication Number Publication Date
JPS56164787A true JPS56164787A (en) 1981-12-17

Family

ID=13361656

Family Applications (1)

Application Number Title Priority Date Filing Date
JP6801880A Pending JPS56164787A (en) 1979-11-22 1980-05-21 Production of heat-resistant pyruvate kinase

Country Status (1)

Country Link
JP (1) JPS56164787A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01503596A (en) * 1987-04-10 1989-12-07 ザ　フリンダーズ　ユニヴアーシテイ　オブ　サウス　オーストラリア Method for measuring potassium ions in body fluids and compositions therefor

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01503596A (en) * 1987-04-10 1989-12-07 ザ　フリンダーズ　ユニヴアーシテイ　オブ　サウス　オーストラリア Method for measuring potassium ions in body fluids and compositions therefor

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