JPS56151357A - Immunoassay method - Google Patents

Immunoassay method

Info

Publication number
JPS56151357A
JPS56151357A JP5415780A JP5415780A JPS56151357A JP S56151357 A JPS56151357 A JP S56151357A JP 5415780 A JP5415780 A JP 5415780A JP 5415780 A JP5415780 A JP 5415780A JP S56151357 A JPS56151357 A JP S56151357A
Authority
JP
Japan
Prior art keywords
substrate
measured
antigens
fine particles
tested
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP5415780A
Other languages
Japanese (ja)
Inventor
Daizo Tokinaga
Teruaki Kobayashi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hitachi Ltd
Original Assignee
Hitachi Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi Ltd filed Critical Hitachi Ltd
Priority to JP5415780A priority Critical patent/JPS56151357A/en
Publication of JPS56151357A publication Critical patent/JPS56151357A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PURPOSE:To enable a simultaneous measurement of a large number of items and to permit automation of said measurement, by a method wherein antibodies against an object to be measured are secured on a substrate. CONSTITUTION:A substrate 1, which has atinbodies 2 against an object to be measured, secured on the surface thereof, is prepared, and a liquid to be tested, containing antigens 3 being the object to be measured, is brought into contact with the substrate for a specific period of time. This causes absorption of antigens in the liquid to be tested to the substrate through the working of the antibodies. After the substrate is well washed, it is brought into contact with a suspension which is prepared separately from the above and suspends fine particles 4 having antibodies 5 against the object to be measured on the surface of the fine particle, and this causes reaction of the antigens, absorbed on the substrate, to the antibodies on the surfaces of the fine particles. After a given period of time has elapsed, unreacted suspension fine particles are washed out of the substrate to prepare a specimen on which the number of the fine particles, corresponding to the amount and the density of the antigens in the liquid to be tested, are secured. The number of the fine particles on the specimen are measured, and this finds the amount and density of the antigens in the liquid to be tested.
JP5415780A 1980-04-25 1980-04-25 Immunoassay method Pending JPS56151357A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP5415780A JPS56151357A (en) 1980-04-25 1980-04-25 Immunoassay method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP5415780A JPS56151357A (en) 1980-04-25 1980-04-25 Immunoassay method

Publications (1)

Publication Number Publication Date
JPS56151357A true JPS56151357A (en) 1981-11-24

Family

ID=12962704

Family Applications (1)

Application Number Title Priority Date Filing Date
JP5415780A Pending JPS56151357A (en) 1980-04-25 1980-04-25 Immunoassay method

Country Status (1)

Country Link
JP (1) JPS56151357A (en)

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5814057A (en) * 1981-07-17 1983-01-26 Toray Ind Inc Measurement of biologically active substances and labelling agent used therefor
JPS5860256A (en) * 1981-10-06 1983-04-09 Toray Ind Inc Measurement of biologically active substance and label agent used therefor
JPS5975152A (en) * 1982-10-23 1984-04-27 Tatsuo Suzuta Fluorescent immunity latex particle
JPS59122950A (en) * 1982-12-28 1984-07-16 Toray Ind Inc Method for measuring biologically active substance and labeling agent used therein
JPS60253870A (en) * 1984-05-08 1985-12-14 Sekisui Chem Co Ltd Immunological diagnosis
JPS6148764A (en) * 1984-08-14 1986-03-10 デューク　サイエンティフィック　コーポレーション Method and kit for testing aqueous sample for specific substance
WO1986007463A1 (en) * 1985-06-03 1986-12-18 American National Red Cross Microtiter - surface - flocculation assay for antigen or antibody screening
JPS628056A (en) * 1985-07-05 1987-01-16 Yoichi Shibata Specific bonding analysis
US4663277A (en) * 1983-05-20 1987-05-05 Profile Diagnostic Sciences Inc. Virus detection method and materials
US5061237A (en) * 1985-07-02 1991-10-29 Cytomed Medizintechnik Gmbh Method of purifying whole blood
WO1992006379A1 (en) * 1990-10-09 1992-04-16 Idemitsu Petrochemical Co., Ltd. Method of immunological quantitative analysis
US5308990A (en) * 1991-05-15 1994-05-03 Hitachi, Ltd. Method for measuring microparticles, quantitative measuring method therefor and instrument for measuring microparticles
JP2007262525A (en) * 2006-03-29 2007-10-11 Jfe Steel Kk Method and apparatus for removing dust of converter exhaust gas treatment facility

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5814057A (en) * 1981-07-17 1983-01-26 Toray Ind Inc Measurement of biologically active substances and labelling agent used therefor
JPS5860256A (en) * 1981-10-06 1983-04-09 Toray Ind Inc Measurement of biologically active substance and label agent used therefor
JPS5975152A (en) * 1982-10-23 1984-04-27 Tatsuo Suzuta Fluorescent immunity latex particle
JPS59122950A (en) * 1982-12-28 1984-07-16 Toray Ind Inc Method for measuring biologically active substance and labeling agent used therein
US4663277A (en) * 1983-05-20 1987-05-05 Profile Diagnostic Sciences Inc. Virus detection method and materials
JPS60253870A (en) * 1984-05-08 1985-12-14 Sekisui Chem Co Ltd Immunological diagnosis
JPS6148764A (en) * 1984-08-14 1986-03-10 デューク　サイエンティフィック　コーポレーション Method and kit for testing aqueous sample for specific substance
WO1986007463A1 (en) * 1985-06-03 1986-12-18 American National Red Cross Microtiter - surface - flocculation assay for antigen or antibody screening
US5061237A (en) * 1985-07-02 1991-10-29 Cytomed Medizintechnik Gmbh Method of purifying whole blood
JPS628056A (en) * 1985-07-05 1987-01-16 Yoichi Shibata Specific bonding analysis
WO1992006379A1 (en) * 1990-10-09 1992-04-16 Idemitsu Petrochemical Co., Ltd. Method of immunological quantitative analysis
US5308990A (en) * 1991-05-15 1994-05-03 Hitachi, Ltd. Method for measuring microparticles, quantitative measuring method therefor and instrument for measuring microparticles
JP2007262525A (en) * 2006-03-29 2007-10-11 Jfe Steel Kk Method and apparatus for removing dust of converter exhaust gas treatment facility

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