JPS56148057A - Acid material analyzer by high speed liquid chromatography - Google Patents
Acid material analyzer by high speed liquid chromatographyInfo
- Publication number
- JPS56148057A JPS56148057A JP5174280A JP5174280A JPS56148057A JP S56148057 A JPS56148057 A JP S56148057A JP 5174280 A JP5174280 A JP 5174280A JP 5174280 A JP5174280 A JP 5174280A JP S56148057 A JPS56148057 A JP S56148057A
- Authority
- JP
- Japan
- Prior art keywords
- indicator
- range
- solution
- phthalene
- monochrome
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/84—Preparation of the fraction to be distributed
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
PURPOSE:To obtain the captioned analyzer having higher detection sensibility, specificity, reproducibility, responsibility and proper universality by injection of sulfone phthalene pH indicator between a separation column and a detecting means. CONSTITUTION:Some eluant is supplied from a vessel 1 to a separation column 5 through a metering pump P. The sample to be tested is injected from an injector 4. The solution (including the sample by solution action) flowing out of the column 5, is joinned together with thin water solution of sulhone phthalene pH indicator which is sent in meterring through a resistor 10 from a vessel 9 by a pump P and is measured by a visible spectrophotometer 6 to find its absorbance, and its chromatogram is recorded on a recroder 7. The pH indicator is slightly soluble in water, has its transition interval for the range of approximate pH3.0-10.0, and further shows the minimum light transmissivity for the monochrome of wave length ranging approximately 420-450nm under the lower pH range than 3.0 and still further the indicator shows the minimum light transmissisivity for the monochrome of wave length ranging higher than approximate 558nm under the higher pH range than 10.0
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5174280A JPS56148057A (en) | 1980-04-21 | 1980-04-21 | Acid material analyzer by high speed liquid chromatography |
| JP17002188A JPH03183948A (en) | 1980-04-21 | 1988-07-09 | Analysis of acidic material by high performance liquid chromatography |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5174280A JPS56148057A (en) | 1980-04-21 | 1980-04-21 | Acid material analyzer by high speed liquid chromatography |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP17002188A Division JPH03183948A (en) | 1980-04-21 | 1988-07-09 | Analysis of acidic material by high performance liquid chromatography |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS56148057A true JPS56148057A (en) | 1981-11-17 |
| JPS6129668B2 JPS6129668B2 (en) | 1986-07-08 |
Family
ID=12895362
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5174280A Granted JPS56148057A (en) | 1980-04-21 | 1980-04-21 | Acid material analyzer by high speed liquid chromatography |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS56148057A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59147262A (en) * | 1983-02-12 | 1984-08-23 | Japan Spectroscopic Co | Analysis of organic acid |
| JPS62121362A (en) * | 1985-11-21 | 1987-06-02 | マイルス・ラボラトリ−ズ・インコ−ポレ−テツド | Ph indicator consisting of thiophene compound and reagent containing said ph indicator |
| WO2010100914A1 (en) * | 2009-03-04 | 2010-09-10 | 株式会社日立ハイテクノロジーズ | Liquid chromatograph and analysis method |
-
1980
- 1980-04-21 JP JP5174280A patent/JPS56148057A/en active Granted
Non-Patent Citations (4)
| Title |
|---|
| ANNAMALES DE BIOLOGIE CLINIQE=1978 * |
| ANNAMMALES DE BIOLOGIE CLINIQE=1978 * |
| CHEMISTRY AND INDUSTRY=1967 * |
| NISHIO NEWS LETTER=1976 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59147262A (en) * | 1983-02-12 | 1984-08-23 | Japan Spectroscopic Co | Analysis of organic acid |
| JPS62121362A (en) * | 1985-11-21 | 1987-06-02 | マイルス・ラボラトリ−ズ・インコ−ポレ−テツド | Ph indicator consisting of thiophene compound and reagent containing said ph indicator |
| WO2010100914A1 (en) * | 2009-03-04 | 2010-09-10 | 株式会社日立ハイテクノロジーズ | Liquid chromatograph and analysis method |
| JPWO2010100914A1 (en) * | 2009-03-04 | 2012-09-06 | 株式会社日立ハイテクノロジーズ | Liquid chromatograph and analysis method |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6129668B2 (en) | 1986-07-08 |
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