JPS5574795A - Preparation of bacteriohemagglutinin mha no.101 - Google Patents

Preparation of bacteriohemagglutinin mha no.101

Info

Publication number
JPS5574795A
JPS5574795A JP14729178A JP14729178A JPS5574795A JP S5574795 A JPS5574795 A JP S5574795A JP 14729178 A JP14729178 A JP 14729178A JP 14729178 A JP14729178 A JP 14729178A JP S5574795 A JPS5574795 A JP S5574795A
Authority
JP
Japan
Prior art keywords
bacteriohemagglutinin
mha
pseudomonas
producing
medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP14729178A
Other languages
Japanese (ja)
Other versions
JPS6046956B2 (en
Inventor
Hiroshi Aida
Kunio Oishi
Soji Yokoshima
Tetsuo Tomiyama
Hiromi Shibuya
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Asahi Chemical Industry Co Ltd
Original Assignee
Asahi Chemical Industry Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asahi Chemical Industry Co Ltd filed Critical Asahi Chemical Industry Co Ltd
Priority to JP53147291A priority Critical patent/JPS6046956B2/en
Publication of JPS5574795A publication Critical patent/JPS5574795A/en
Publication of JPS6046956B2 publication Critical patent/JPS6046956B2/en
Expired legal-status Critical Current

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

PURPOSE: To prepare bacteriohemagglutinin MHA No.101 useful as a drug, by culturing bacteria belonging to Pseudomonas genus and capable of producing the bacteriohemagglutinin MHA No.101.
CONSTITUTION: Bacteria belonging to Pseudomonas genus and capable of producing bacteriohemagglutinin HMA No.101, e.g. Pseudomonas sp. No.101, are inoculated in a liquid medium containing an organic nitrogen source as major constituent, and cultured under an aerobic condition, at about 20W37°C for about 24 hours to accumulate the objective bacteriohemagglutinin MHA No.101 in the medium. After removing the bacterial cells from the medium, the filtrate is slated out with ammonium sulfate, and the precipitate is dissolved in a phosphate buffer solution. The desired bacteriohemagglutinin MHA No.101 is obtained by purifying the solution with DEAE-Sephadex A-50.
COPYRIGHT: (C)1980,JPO&Japio
JP53147291A 1978-11-30 1978-11-30 Microbial hemagglutinin MHAN No. 101 manufacturing methods Expired JPS6046956B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP53147291A JPS6046956B2 (en) 1978-11-30 1978-11-30 Microbial hemagglutinin MHAN No. 101 manufacturing methods

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP53147291A JPS6046956B2 (en) 1978-11-30 1978-11-30 Microbial hemagglutinin MHAN No. 101 manufacturing methods

Publications (2)

Publication Number Publication Date
JPS5574795A true JPS5574795A (en) 1980-06-05
JPS6046956B2 JPS6046956B2 (en) 1985-10-18

Family

ID=15426877

Family Applications (1)

Application Number Title Priority Date Filing Date
JP53147291A Expired JPS6046956B2 (en) 1978-11-30 1978-11-30 Microbial hemagglutinin MHAN No. 101 manufacturing methods

Country Status (1)

Country Link
JP (1) JPS6046956B2 (en)

Also Published As

Publication number Publication date
JPS6046956B2 (en) 1985-10-18

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