JPS5574795A - Preparation of bacteriohemagglutinin mha no.101 - Google Patents
Preparation of bacteriohemagglutinin mha no.101Info
- Publication number
- JPS5574795A JPS5574795A JP14729178A JP14729178A JPS5574795A JP S5574795 A JPS5574795 A JP S5574795A JP 14729178 A JP14729178 A JP 14729178A JP 14729178 A JP14729178 A JP 14729178A JP S5574795 A JPS5574795 A JP S5574795A
- Authority
- JP
- Japan
- Prior art keywords
- bacteriohemagglutinin
- mha
- pseudomonas
- producing
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
PURPOSE: To prepare bacteriohemagglutinin MHA No.101 useful as a drug, by culturing bacteria belonging to Pseudomonas genus and capable of producing the bacteriohemagglutinin MHA No.101.
CONSTITUTION: Bacteria belonging to Pseudomonas genus and capable of producing bacteriohemagglutinin HMA No.101, e.g. Pseudomonas sp. No.101, are inoculated in a liquid medium containing an organic nitrogen source as major constituent, and cultured under an aerobic condition, at about 20W37°C for about 24 hours to accumulate the objective bacteriohemagglutinin MHA No.101 in the medium. After removing the bacterial cells from the medium, the filtrate is slated out with ammonium sulfate, and the precipitate is dissolved in a phosphate buffer solution. The desired bacteriohemagglutinin MHA No.101 is obtained by purifying the solution with DEAE-Sephadex A-50.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP53147291A JPS6046956B2 (en) | 1978-11-30 | 1978-11-30 | Microbial hemagglutinin MHAN No. 101 manufacturing methods |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP53147291A JPS6046956B2 (en) | 1978-11-30 | 1978-11-30 | Microbial hemagglutinin MHAN No. 101 manufacturing methods |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5574795A true JPS5574795A (en) | 1980-06-05 |
JPS6046956B2 JPS6046956B2 (en) | 1985-10-18 |
Family
ID=15426877
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP53147291A Expired JPS6046956B2 (en) | 1978-11-30 | 1978-11-30 | Microbial hemagglutinin MHAN No. 101 manufacturing methods |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6046956B2 (en) |
-
1978
- 1978-11-30 JP JP53147291A patent/JPS6046956B2/en not_active Expired
Also Published As
Publication number | Publication date |
---|---|
JPS6046956B2 (en) | 1985-10-18 |
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