JPS5536740A - Fluorescent estimation of phenylthiohydantoic amino acid - Google Patents

Fluorescent estimation of phenylthiohydantoic amino acid

Info

Publication number
JPS5536740A
JPS5536740A JP10960278A JP10960278A JPS5536740A JP S5536740 A JPS5536740 A JP S5536740A JP 10960278 A JP10960278 A JP 10960278A JP 10960278 A JP10960278 A JP 10960278A JP S5536740 A JPS5536740 A JP S5536740A
Authority
JP
Japan
Prior art keywords
amino acid
phenylthiohydantoic
fluorescent
pht
zinc acetate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP10960278A
Other languages
Japanese (ja)
Inventor
Toshio Kinoshita
Kazuyo Murayama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
YUKI GOSEI YAKUHIN KOGYO KK
Original Assignee
YUKI GOSEI YAKUHIN KOGYO KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by YUKI GOSEI YAKUHIN KOGYO KK filed Critical YUKI GOSEI YAKUHIN KOGYO KK
Priority to JP10960278A priority Critical patent/JPS5536740A/en
Publication of JPS5536740A publication Critical patent/JPS5536740A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

PURPOSE:To estimate phenylthiohydantoic amino acid quickly and accurately in simple operation by generating fluorescent light by reacting phenylthiohydantoic amino acid with pyridoxamine and zinc acetate. CONSTITUTION:Pyridoxine and zinc acetate are reacted with phenylthiohydantoic (PTA) amino acid which is produced by Edman's decomposition of peptide or protein, and fluorescent light is generated, thereby PHT amino acid is estimated. For example, 250mug of pyridoxamine and 5ml of 2% pyridine methanol solution containing 250mg zinc acetate are added to 0.5ml of ethyl acetate containing 0.124mug of PHT-norleucine and churned. After the product is heated for 90 minutes at 70 deg.C, fluorescent light is generated. Then, it is cooled for 5 minutes by ice bathing, and measured by an excitation wavelength of 390nm and a fluorescent-light wavelength of 465nm, thereby a spectrum of PHT-amino-acid as shown by the pattern in the Figure is obtained.
JP10960278A 1978-09-08 1978-09-08 Fluorescent estimation of phenylthiohydantoic amino acid Pending JPS5536740A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP10960278A JPS5536740A (en) 1978-09-08 1978-09-08 Fluorescent estimation of phenylthiohydantoic amino acid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP10960278A JPS5536740A (en) 1978-09-08 1978-09-08 Fluorescent estimation of phenylthiohydantoic amino acid

Publications (1)

Publication Number Publication Date
JPS5536740A true JPS5536740A (en) 1980-03-14

Family

ID=14514432

Family Applications (1)

Application Number Title Priority Date Filing Date
JP10960278A Pending JPS5536740A (en) 1978-09-08 1978-09-08 Fluorescent estimation of phenylthiohydantoic amino acid

Country Status (1)

Country Link
JP (1) JPS5536740A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104610116A (en) * 2015-02-21 2015-05-13 桂林理工大学 Bis-phenyl thiourea derivative and application thereof
CN104610117A (en) * 2015-02-21 2015-05-13 桂林理工大学 Bis-phenylthiourea and application thereof
CN109696411A (en) * 2019-02-01 2019-04-30 天津城建大学 A kind of water from refined p-benzene dicarboxylic acid production dissolubility COD rapid detection method

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104610116A (en) * 2015-02-21 2015-05-13 桂林理工大学 Bis-phenyl thiourea derivative and application thereof
CN104610117A (en) * 2015-02-21 2015-05-13 桂林理工大学 Bis-phenylthiourea and application thereof
CN109696411A (en) * 2019-02-01 2019-04-30 天津城建大学 A kind of water from refined p-benzene dicarboxylic acid production dissolubility COD rapid detection method

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