JPS5534007A - Determination of amylase activity - Google Patents

Determination of amylase activity

Info

Publication number
JPS5534007A
JPS5534007A JP10395378A JP10395378A JPS5534007A JP S5534007 A JPS5534007 A JP S5534007A JP 10395378 A JP10395378 A JP 10395378A JP 10395378 A JP10395378 A JP 10395378A JP S5534007 A JPS5534007 A JP S5534007A
Authority
JP
Japan
Prior art keywords
glucose
amylase activity
acid
maltose
determination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP10395378A
Other languages
Japanese (ja)
Other versions
JPS5733956B2 (en
Inventor
Isamu Kokawara
Osamu Oka
Takeshi Fujita
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oriental Yeast Co Ltd
Original Assignee
Oriental Yeast Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oriental Yeast Co Ltd filed Critical Oriental Yeast Co Ltd
Priority to JP10395378A priority Critical patent/JPS5534007A/en
Publication of JPS5534007A publication Critical patent/JPS5534007A/en
Publication of JPS5733956B2 publication Critical patent/JPS5733956B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: To determine the amylase activity with ease and accuracy, by converting maltose and glucose in the specimen with an enzyme system into 6-phosphogluconic acid, and by reacting the acid with dextrin, etc. and NADP+ to increase the NADPH.
CONSTITUTION: An enzyme system comprising maltase, hexokinase, glucose-6- phosphate dehydrogenase, an enzyme for oxidizing NAD+ and NADH, and a substrate for oxidizing NADH, is added to remove the maltose and (or) glucose from the specimen. The maltose and glucose are thus converted into 6-phosphogluconic acid. One or more types of starch, dextrin, amylose, and amylopectin in excess and NADP+ are reacted with the acid to determine the amylase activity from the increase in NADPH with an absorbance at 340nm.
COPYRIGHT: (C)1980,JPO&Japio
JP10395378A 1978-08-28 1978-08-28 Determination of amylase activity Granted JPS5534007A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP10395378A JPS5534007A (en) 1978-08-28 1978-08-28 Determination of amylase activity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP10395378A JPS5534007A (en) 1978-08-28 1978-08-28 Determination of amylase activity

Publications (2)

Publication Number Publication Date
JPS5534007A true JPS5534007A (en) 1980-03-10
JPS5733956B2 JPS5733956B2 (en) 1982-07-20

Family

ID=14367771

Family Applications (1)

Application Number Title Priority Date Filing Date
JP10395378A Granted JPS5534007A (en) 1978-08-28 1978-08-28 Determination of amylase activity

Country Status (1)

Country Link
JP (1) JPS5534007A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0670795A (en) * 1992-06-30 1994-03-15 Nitto Boseki Co Ltd Determination of 1,5-anhydroglucitol

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0670795A (en) * 1992-06-30 1994-03-15 Nitto Boseki Co Ltd Determination of 1,5-anhydroglucitol
JPH0767395B2 (en) * 1992-06-30 1995-07-26 日東紡績株式会社 Method for quantifying 1,5-anhydroglucitol

Also Published As

Publication number Publication date
JPS5733956B2 (en) 1982-07-20

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