JPS5524103A - Tetrazolium salt compound and absorption photometry of dehydrogenase using the same - Google Patents

Tetrazolium salt compound and absorption photometry of dehydrogenase using the same

Info

Publication number
JPS5524103A
JPS5524103A JP9586478A JP9586478A JPS5524103A JP S5524103 A JPS5524103 A JP S5524103A JP 9586478 A JP9586478 A JP 9586478A JP 9586478 A JP9586478 A JP 9586478A JP S5524103 A JPS5524103 A JP S5524103A
Authority
JP
Japan
Prior art keywords
dehydrogenase
formula
absorption photometry
same
salt compound
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP9586478A
Other languages
Japanese (ja)
Other versions
JPS5638154B2 (en
Inventor
Hisaya Kataoka
Masayasu Maeda
Keisuke Ueno
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DOUJIN YATSUKAGAKU KENKYUSHO G
DOUJIN YATSUKAGAKU KENKYUSHO GOUSHI
Original Assignee
DOUJIN YATSUKAGAKU KENKYUSHO G
DOUJIN YATSUKAGAKU KENKYUSHO GOUSHI
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DOUJIN YATSUKAGAKU KENKYUSHO G, DOUJIN YATSUKAGAKU KENKYUSHO GOUSHI filed Critical DOUJIN YATSUKAGAKU KENKYUSHO G
Priority to JP9586478A priority Critical patent/JPS5524103A/en
Publication of JPS5524103A publication Critical patent/JPS5524103A/en
Publication of JPS5638154B2 publication Critical patent/JPS5638154B2/ja
Granted legal-status Critical Current

Links

Abstract

NEW MATERIAL:The free acid of a monotetrazolium salt of formula (when R1 is sulfone, R2 is H; when R1 is H, R2 is carboxy; X is Cl or Br), wherein its 5-phenyl group is substituted at 2- or 4-position; or its alkali metal salt.
EXAMPLE: 3-(4-Iodophenyl)-2-(4-nitrophenyl)-5-(4-carboxyphenyl)-2H tetrazolium chloride (R1=H and R2=COOH in the formula).
USE: Useful as a hydrogen-acceptor in absorption photometry of a dehydrogenase. For example, lactic acid dehydrogenase in blood is determined for the diagnosis of a disease of heart muscle, liver, etc.. It has improved solubility and gives reproducible results.
PREPARATION: For example, a 2- or 4-substituted benzaldehyde is reacted with 4-nitrophenylhydrazine in an organic solvent. The product is mixed with 4-iodophenyldiazonium chloride under basic conditions, and oxidized under acidic conditions to give the compound expressed by the formula.
COPYRIGHT: (C)1980,JPO&Japio
JP9586478A 1978-08-08 1978-08-08 Tetrazolium salt compound and absorption photometry of dehydrogenase using the same Granted JPS5524103A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP9586478A JPS5524103A (en) 1978-08-08 1978-08-08 Tetrazolium salt compound and absorption photometry of dehydrogenase using the same

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP9586478A JPS5524103A (en) 1978-08-08 1978-08-08 Tetrazolium salt compound and absorption photometry of dehydrogenase using the same

Publications (2)

Publication Number Publication Date
JPS5524103A true JPS5524103A (en) 1980-02-21
JPS5638154B2 JPS5638154B2 (en) 1981-09-04

Family

ID=14149218

Family Applications (1)

Application Number Title Priority Date Filing Date
JP9586478A Granted JPS5524103A (en) 1978-08-08 1978-08-08 Tetrazolium salt compound and absorption photometry of dehydrogenase using the same

Country Status (1)

Country Link
JP (1) JPS5524103A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62249979A (en) * 1986-04-04 1987-10-30 ベ−リンガ−・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング Method and reagent for measuring substrate or enzyme activity
CN103980216A (en) * 2014-06-05 2014-08-13 湖北百诺捷生物科技有限公司 Synthesis method of chloridized-2,3,5-triphenyl tetrazolium chloride
CN105418526A (en) * 2015-11-09 2016-03-23 杭州伽玛生物科技有限公司 Compound with mono-sulfophenyl tetrazole structure and application of compound with mono-sulfophenyl tetrazole structure

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59162918U (en) * 1983-04-18 1984-10-31 株式会社共立 air cleaner

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62249979A (en) * 1986-04-04 1987-10-30 ベ−リンガ−・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング Method and reagent for measuring substrate or enzyme activity
JPH0445510B2 (en) * 1986-04-04 1992-07-27 Boehringer Mannheim Gmbh
CN103980216A (en) * 2014-06-05 2014-08-13 湖北百诺捷生物科技有限公司 Synthesis method of chloridized-2,3,5-triphenyl tetrazolium chloride
CN105418526A (en) * 2015-11-09 2016-03-23 杭州伽玛生物科技有限公司 Compound with mono-sulfophenyl tetrazole structure and application of compound with mono-sulfophenyl tetrazole structure
WO2017080443A1 (en) * 2015-11-09 2017-05-18 Hangzhou Jennifer Biotech Co., Ltd. Monosulfonic phenyltetrazole compounds with applications
US20180244637A1 (en) * 2015-11-09 2018-08-30 Hangzhou Jennifer Biotech Co., Ltd. Monosulfonic phenyltetrazole compounds with applications
JP2019502655A (en) * 2015-11-09 2019-01-31 ハンジョウ ジェニファー バイオーテック カンパニー リミテッドＨａｎｇｚｈｏｕ Ｊｅｎｎｉｆｅｒ Ｂｉｏｔｅｃｈ Ｃｏ．，Ｌｔｄ． Monosulfonic acid phenyltetrazole compounds and applications
US10597369B2 (en) 2015-11-09 2020-03-24 Hangzhou Jennifer Biotech Co., Ltd. Monosulfonic phenyltetrazole compounds with applications

Also Published As

Publication number Publication date
JPS5638154B2 (en) 1981-09-04

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