Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
YUKI GOSEI YAKUHIN KOGYO KK
Original Assignee
YUKI GOSEI YAKUHIN KOGYO KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by YUKI GOSEI YAKUHIN KOGYO KKfiledCriticalYUKI GOSEI YAKUHIN KOGYO KK
Priority to JP1803279ApriorityCriticalpatent/JPS55110938A/en
Publication of JPS55110938ApublicationCriticalpatent/JPS55110938A/en
G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
Abstract
PURPOSE:To carry out easily and conveniently fluorometric determination of glutathione by using a specific salt of aminonaphthalenesulfonamide. CONSTITUTION:Fluorometric analysis is carried out by using as a reagent sodium N-chloro-1-dimethylaminonaphthalene-5-sulfonamide (DNS-NCl.Na). Morespecifically, a sample to be examined is added to a methanol solution (concentration of about 0.01-0.0125%) of DNS-NCl.Na, and the mixture is agitated. Emitted fluorescent light is measured under and excitation wavelength of 345nm and fluorescence wavelength of 515nm.
JP1803279A1979-02-191979-02-19Method of fluorometric determination of glutathione
PendingJPS55110938A
(en)
Kinetics and stoichiometry of light-induced proton release and uptake from purple membrane fragments, Halobacterium halobium cell envelopes, and phospholipid vesicles containing oriented purple membrane