JPS55110937A - Method of fluometric quantative analysis of amino acid, peptide or protein using sodium n-chloro-1- dimethylaminonaphthalene-5-sulfonamide - Google Patents

Method of fluometric quantative analysis of amino acid, peptide or protein using sodium n-chloro-1- dimethylaminonaphthalene-5-sulfonamide

Info

Publication number
JPS55110937A
JPS55110937A JP1803179A JP1803179A JPS55110937A JP S55110937 A JPS55110937 A JP S55110937A JP 1803179 A JP1803179 A JP 1803179A JP 1803179 A JP1803179 A JP 1803179A JP S55110937 A JPS55110937 A JP S55110937A
Authority
JP
Japan
Prior art keywords
fluometric
sulfonamide
dimethylaminonaphthalene
chloro
peptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP1803179A
Other languages
Japanese (ja)
Inventor
Toshio Kinoshita
Kazuyo Murayama
Takashi Nishi
Tokuji Goto
Tsutomu Nakao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
YUKI GOSEI YAKUHIN KOGYO KK
Original Assignee
YUKI GOSEI YAKUHIN KOGYO KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by YUKI GOSEI YAKUHIN KOGYO KK filed Critical YUKI GOSEI YAKUHIN KOGYO KK
Priority to JP1803179A priority Critical patent/JPS55110937A/en
Publication of JPS55110937A publication Critical patent/JPS55110937A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence

Landscapes

  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

PURPOSE:To carry out a fluometric quantative analysis of amino acid, peptide or the like easily with high sensitivity and accuracy by using a specific salt of aminonaphthalene sulfonamide. CONSTITUTION:The sample to be examined is subjected to fluometric analysis using as a reagent sodium N-chloro-1-dimethylaminonaphthalene-5-sulfonamide (DNS-NCl.Na). More particularly, the sample to be examined is added to a methanol solution (concentration of about 0.01% or more) of DNS-NCl.Na, and the mixture solution is agitated and heated at about 60 deg.C for about 20 minutes. Then, the mixture solution is cooled for about 3 minutes in an ice bath. Thereafter, generated fluorescent light is measured under an excitation wavelength of 345nm, and fluorescence waveform of 515nm.
JP1803179A 1979-02-19 1979-02-19 Method of fluometric quantative analysis of amino acid, peptide or protein using sodium n-chloro-1- dimethylaminonaphthalene-5-sulfonamide Pending JPS55110937A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP1803179A JPS55110937A (en) 1979-02-19 1979-02-19 Method of fluometric quantative analysis of amino acid, peptide or protein using sodium n-chloro-1- dimethylaminonaphthalene-5-sulfonamide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP1803179A JPS55110937A (en) 1979-02-19 1979-02-19 Method of fluometric quantative analysis of amino acid, peptide or protein using sodium n-chloro-1- dimethylaminonaphthalene-5-sulfonamide

Publications (1)

Publication Number Publication Date
JPS55110937A true JPS55110937A (en) 1980-08-27

Family

ID=11960289

Family Applications (1)

Application Number Title Priority Date Filing Date
JP1803179A Pending JPS55110937A (en) 1979-02-19 1979-02-19 Method of fluometric quantative analysis of amino acid, peptide or protein using sodium n-chloro-1- dimethylaminonaphthalene-5-sulfonamide

Country Status (1)

Country Link
JP (1) JPS55110937A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102603649A (en) * 2011-01-20 2012-07-25 赣南师范学院 Fluorescence probe compound taking dibazole as guide molecule and preparation method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102603649A (en) * 2011-01-20 2012-07-25 赣南师范学院 Fluorescence probe compound taking dibazole as guide molecule and preparation method thereof
CN102603649B (en) * 2011-01-20 2016-05-25 赣南师范学院 A kind of fluorescent probe compounds taking dibazol as guide's molecule and preparation method thereof

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