JPS5455791A - Extraction of ribouncleic acid - Google Patents
Extraction of ribouncleic acidInfo
- Publication number
- JPS5455791A JPS5455791A JP11948377A JP11948377A JPS5455791A JP S5455791 A JPS5455791 A JP S5455791A JP 11948377 A JP11948377 A JP 11948377A JP 11948377 A JP11948377 A JP 11948377A JP S5455791 A JPS5455791 A JP S5455791A
- Authority
- JP
- Japan
- Prior art keywords
- dispersion
- rna
- nacl
- aqueous solution
- alkaline aqueous
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
PURPOSE: To prepare ribonucleic acid in high efficiency, by extracting specific novel yeast with hot neutral or alkaline aqueous solution of NaCl.
CONSTITUTION: Pure ribonucleic acdi (RNA) is prepared by (1) dispersing 5- 10wt% of yeast obtained by the cultivation of Candida tropicalis C-1151 (FERM- P No. 4173), in water, (2) adding about 1-15 wt% of NaCl to the dispersion, (3) adding an alkaline aqueous solution to the dispersion with stirring to adjust the pH value between 7 and 8 (pref. 7.5 and 7.8), (4) heating the dispersion at about 70-100°C for 30-200 minutes to extract the RNA, (5) removing the extraction residue by centrifugal separation, (6) adding about 0.5-5 wt% of CaCl2 to the extract containing RNA, (7) adjusting the pH of the solution to ≤ 2, by adding HCl and (8) separating the precipitate
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11948377A JPS5455791A (en) | 1977-10-06 | 1977-10-06 | Extraction of ribouncleic acid |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11948377A JPS5455791A (en) | 1977-10-06 | 1977-10-06 | Extraction of ribouncleic acid |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5455791A true JPS5455791A (en) | 1979-05-04 |
JPS5549838B2 JPS5549838B2 (en) | 1980-12-15 |
Family
ID=14762391
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP11948377A Granted JPS5455791A (en) | 1977-10-06 | 1977-10-06 | Extraction of ribouncleic acid |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5455791A (en) |
-
1977
- 1977-10-06 JP JP11948377A patent/JPS5455791A/en active Granted
Also Published As
Publication number | Publication date |
---|---|
JPS5549838B2 (en) | 1980-12-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101870722B (en) | Process for concentrating protein in soy protein wastewater by two-stage foam separation method | |
CN101803724B (en) | Preparation method of Semen coicis hydrolysate | |
CN103342669A (en) | Simple method for extracting natural taurine | |
US4122196A (en) | Process for the manufacture of yeast glycan | |
JPS5716649A (en) | Method of making oolong tea drink | |
JPS57206391A (en) | Preparation of flavonoid natural pigment by enzymic treatment | |
CN104495754B (en) | A kind of extracting method of Iodine | |
US3127388A (en) | Peroxide treatment in alkaline and en- | |
JPS5455791A (en) | Extraction of ribouncleic acid | |
JPS561888A (en) | Preparation of concentrated aqueous solution of acrylamide with microorganism | |
GB1298431A (en) | Process for the enrichment of proteins | |
US7135327B2 (en) | Ribonucleic acid-enriched brewer's yeast cells and process for producing the same | |
US3686144A (en) | Recovery of yeast proteins in refined form and with high yield by dehydration with an alkanol followed by acidic esterification | |
CN109666085A (en) | A method of pectin from lemon peel is purified using alcohol precipitation | |
Van der Walt et al. | Ploidy differences in Sporobolomyces salmonicolor and Candida albicans | |
JPS5771376A (en) | Preparation of yeast extract | |
WO2002026966A3 (en) | Transfection using plasmid preparations | |
CN1089618A (en) | To produce the method for vegetable-protein in the cottonseed cake | |
JPS5663920A (en) | Arrow root extract and production of its medical preparation | |
JPS5672661A (en) | Preparation of novel seasoning having flavor of shrimp | |
JPS57181671A (en) | Extracting method of extract from hard mushroom | |
Ibuki et al. | Occurence of a Phosphodiesterase in the Insoluble Fractions from Wheat Seedlings | |
Walt et al. | Ploidy differences inSporobolomyces salmonicolor andCandida albicans | |
JPS61181394A (en) | Method of isolating and collecting ribonucleic acid | |
SU611926A1 (en) | Method of obtaining hop extract |