WO2002026966A3 - Transfection using plasmid preparations - Google Patents

Transfection using plasmid preparations Download PDF

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Publication number
WO2002026966A3
WO2002026966A3 PCT/GB2001/004351 GB0104351W WO0226966A3 WO 2002026966 A3 WO2002026966 A3 WO 2002026966A3 GB 0104351 W GB0104351 W GB 0104351W WO 0226966 A3 WO0226966 A3 WO 0226966A3
Authority
WO
WIPO (PCT)
Prior art keywords
transfection
inventors
plasmid dna
plasmid
plasmid preparations
Prior art date
Application number
PCT/GB2001/004351
Other languages
French (fr)
Other versions
WO2002026966A2 (en
Inventor
Jason Lee Wright
Florian Maria Wurm
Original Assignee
Ecole Polytech
Jason Lee Wright
Florian Maria Wurm
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ecole Polytech, Jason Lee Wright, Florian Maria Wurm filed Critical Ecole Polytech
Priority to AU2001290147A priority Critical patent/AU2001290147A1/en
Publication of WO2002026966A2 publication Critical patent/WO2002026966A2/en
Publication of WO2002026966A3 publication Critical patent/WO2002026966A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

There is provided a method for plasmid DNA extraction to reproducibly yield relatively crude preparation which is suitable for transfection into mammalian cells. The inventors have determined that the high level of purification previously thought to be necessary in order to successfully allow transfection of the plasmid DNA into cells is not required. The inventors have developed a method of producing a transfectable plasmid preparation that can be performed on a large scale that avoids the use of specialized equipment and reagents.
PCT/GB2001/004351 2000-09-29 2001-09-28 Transfection using plasmid preparations WO2002026966A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001290147A AU2001290147A1 (en) 2000-09-29 2001-09-28 Transfection using plasmid preparations

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23666000P 2000-09-29 2000-09-29
US60/236,660 2000-09-29

Publications (2)

Publication Number Publication Date
WO2002026966A2 WO2002026966A2 (en) 2002-04-04
WO2002026966A3 true WO2002026966A3 (en) 2002-10-17

Family

ID=22890443

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2001/004351 WO2002026966A2 (en) 2000-09-29 2001-09-28 Transfection using plasmid preparations

Country Status (2)

Country Link
AU (1) AU2001290147A1 (en)
WO (1) WO2002026966A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8501402B2 (en) 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
EP2088196A1 (en) 2008-02-08 2009-08-12 Boehringer Ingelheim RCV GmbH & Co KG Methods and devices for producing biomolecules
AT509622A1 (en) 2010-04-08 2011-10-15 Greiner Bio One Gmbh METHOD AND KIT FOR THE SEPARATION OF VIRAL AND PROKARYONTIC FROM EUKARYONTIC NUCLEIC ACIDS
JP6683598B2 (en) * 2013-03-15 2020-04-22 マックスサイト インコーポレーティッド Methods and compositions for producing stable transfected cells
EP3283635B1 (en) 2015-04-13 2022-07-06 Maxcyte, Inc. Methods and compositions for modifying genomic dna
IT201700056835A1 (en) * 2017-05-25 2018-11-25 Mascia Brunelli S P A METHOD FOR THE EXTRACTION OF RECOMBINANT PROTEINS

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
FELICIELLO ET AL: "A Modified Alkaline Lysis Method for the Preparation of Highly Purified Plasmid DNA from Escherichia coli", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 212, 1993, pages 394 - 401, XP002093177, ISSN: 0003-2697 *
KAHN DAVID W ET AL: "Purification of plasmid DNA by tangential flow filtration", BIOTECHNOLOGY AND BIOENGINEERING. INCLUDING: SYMPOSIUM BIOTECHNOLOGY IN ENERGY PRODUCTION AND CONSERVATION, JOHN WILEY & SONS. NEW YORK, US, vol. 69, no. 1, 5 July 2000 (2000-07-05), pages 101 - 106, XP002151908, ISSN: 0006-3592 *
LEVY M S ET AL: "Biochemical engineering approaches to the challenges of producing pure plasmid DNA", TRENDS IN BIOTECHNOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 18, no. 7, 1 July 2000 (2000-07-01), pages 296 - 305, XP004213278, ISSN: 0167-7799 *
PRAZERES D M F ET AL: "Large-scale production of pharmaceutical-grade plasmid DNA for gene therapy: problems and bottlenecks", TRENDS IN BIOTECHNOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 17, no. 4, April 1999 (1999-04-01), pages 169 - 174, XP004162836, ISSN: 0167-7799 *

Also Published As

Publication number Publication date
WO2002026966A2 (en) 2002-04-04
AU2001290147A1 (en) 2002-04-08

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