JPS5446886A - Immobilization of enzyme - Google Patents
Immobilization of enzymeInfo
- Publication number
- JPS5446886A JPS5446886A JP11370877A JP11370877A JPS5446886A JP S5446886 A JPS5446886 A JP S5446886A JP 11370877 A JP11370877 A JP 11370877A JP 11370877 A JP11370877 A JP 11370877A JP S5446886 A JPS5446886 A JP S5446886A
- Authority
- JP
- Japan
- Prior art keywords
- enzyme
- solution
- film
- isocyanate
- breaking
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
PURPOSE: To prepare an immobilized enzyme having excellent mechanical strength and water resistance, by mixing a specific isocyanate-terminated prepolymer with an aqueous solution of enzyme, adding a hydrophilic organic solvent to the mixture prior to the completion of the foaming reaction thereby breaking the foam, casting the composition to a film, and finally drying the film.
CONSTITUTION: An isocyanate-terminated prepolymer is prepared by reacting an isocyanate compound with a hydrophilic polyether comprising one or more EO/PO copolymers having an EO content of ≥50% and an average molecular weight of 500W10,000. 0.1W10parts of an aqueous solution of enzyme to be immobilized, is added to 1 part of the prepolymer, and made to react under agitation. When the degree of the foaming reaction reaches 50W70%, 0.1W3 times volume, based on the enzyme solution, of a hydrophilic organic solution such as acetone is added to the reaction mixture thereby reducing the viscosity and breaking the foams. The defoamed solution is cast to a film at room temperature, and made to gel by drying.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11370877A JPS5446886A (en) | 1977-09-20 | 1977-09-20 | Immobilization of enzyme |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11370877A JPS5446886A (en) | 1977-09-20 | 1977-09-20 | Immobilization of enzyme |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5446886A true JPS5446886A (en) | 1979-04-13 |
| JPS5625118B2 JPS5625118B2 (en) | 1981-06-10 |
Family
ID=14619141
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP11370877A Granted JPS5446886A (en) | 1977-09-20 | 1977-09-20 | Immobilization of enzyme |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5446886A (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5819038U (en) * | 1981-07-29 | 1983-02-05 | 株式会社アーレスティ | floating floor panel |
| JPS58186011U (en) * | 1982-06-04 | 1983-12-10 | 桧山 邦夫 | Connection and combination device for side end of wall covering with horizontal insulation material |
-
1977
- 1977-09-20 JP JP11370877A patent/JPS5446886A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5625118B2 (en) | 1981-06-10 |
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