JPS54138182A - Rapid microbiological assay - Google Patents
Rapid microbiological assayInfo
- Publication number
- JPS54138182A JPS54138182A JP4501578A JP4501578A JPS54138182A JP S54138182 A JPS54138182 A JP S54138182A JP 4501578 A JP4501578 A JP 4501578A JP 4501578 A JP4501578 A JP 4501578A JP S54138182 A JPS54138182 A JP S54138182A
- Authority
- JP
- Japan
- Prior art keywords
- vitamins
- amino acids
- determined
- immobilized
- microorganisms
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
PURPOSE: To perform the microbiological assay of vitamins or amino acids, in a short time, without aseptic treatment, by making the vitamins, etc. contact with immobilized microorganisms which require the vitamins, etc., and determining the produced lactic acid.
CONSTITUTION: Microorganisms which require the vitamins or amino acids to be determined as nutrients, e.g. Lactobacillus arabinosus, etc., are immobilized in a polymer gel, e.g. agar gel. The concentrations of the agar gel and the microorganism are pref. 2W2.5 W/V%, and 0.1W1.0%, respectively. A specimen to be determined and the immobilized microorganisms are added to a specific amount of medium, and made to react for 1W2 hours at 15W40°C. The vitamins or amino acids in the specimen are determined by determining the produced lactic acid, pref. by using a glass electrode.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4501578A JPS54138182A (en) | 1978-04-17 | 1978-04-17 | Rapid microbiological assay |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4501578A JPS54138182A (en) | 1978-04-17 | 1978-04-17 | Rapid microbiological assay |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS54138182A true JPS54138182A (en) | 1979-10-26 |
JPS5638120B2 JPS5638120B2 (en) | 1981-09-04 |
Family
ID=12707518
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP4501578A Granted JPS54138182A (en) | 1978-04-17 | 1978-04-17 | Rapid microbiological assay |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS54138182A (en) |
-
1978
- 1978-04-17 JP JP4501578A patent/JPS54138182A/en active Granted
Also Published As
Publication number | Publication date |
---|---|
JPS5638120B2 (en) | 1981-09-04 |
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