JPS54127397A - Liquid chromatography - Google Patents
Liquid chromatographyInfo
- Publication number
- JPS54127397A JPS54127397A JP3460578A JP3460578A JPS54127397A JP S54127397 A JPS54127397 A JP S54127397A JP 3460578 A JP3460578 A JP 3460578A JP 3460578 A JP3460578 A JP 3460578A JP S54127397 A JPS54127397 A JP S54127397A
- Authority
- JP
- Japan
- Prior art keywords
- eluate
- column
- filler
- separation column
- swell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Treatment Of Liquids With Adsorbents In General (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
PURPOSE: To obtain excellent reproducibility of elution time of component such as amino acid of protein hydrolysate, by passing a specific buffer into the passage upstream of separation column before supplying the first stage buffer after supplying regenerating liquid into the separation column.
CONSTITUTION: The first stage eluate B1 runs through a T-pipe 3, to which are connected a solenoid valve V1, manifold 1, pump 2, damper 4 and pressure gauge 5, sampler 6 and separation column 7, and the sample solution of which components are separated is mixed with a coloring reagent 8 which is sent through a pump 9. The mixture develops color in a reaction vessel 10, and the optical characteristics are measured by a photometer 11. Eluates B1 to B3 are successively changed over, and when the elution of amino acid from the column 7 is over, regenerating liquid RG is passed in, so that the column filler is regenerated. After the second eluate B2 of which power to swell the filler is smaller than the eluate B1 which contains the substance to swell the said filler is passed for a certain time, the eluate B1 is permitted to flow so as to balance the column 7. Thus, pressure elevation may be avoided, and the liquid RG may not be left in the pipe 3.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3460578A JPS592861B2 (en) | 1978-03-25 | 1978-03-25 | liquid chromatography |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3460578A JPS592861B2 (en) | 1978-03-25 | 1978-03-25 | liquid chromatography |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS54127397A true JPS54127397A (en) | 1979-10-03 |
| JPS592861B2 JPS592861B2 (en) | 1984-01-20 |
Family
ID=12418982
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP3460578A Expired JPS592861B2 (en) | 1978-03-25 | 1978-03-25 | liquid chromatography |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS592861B2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS586465A (en) * | 1981-07-03 | 1983-01-14 | Sekisui Chem Co Ltd | Eluting method |
| JPS5834359A (en) * | 1981-08-25 | 1983-02-28 | Jeol Ltd | Liquid chromatograph analyzing method |
-
1978
- 1978-03-25 JP JP3460578A patent/JPS592861B2/en not_active Expired
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS586465A (en) * | 1981-07-03 | 1983-01-14 | Sekisui Chem Co Ltd | Eluting method |
| JPS5834359A (en) * | 1981-08-25 | 1983-02-28 | Jeol Ltd | Liquid chromatograph analyzing method |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS592861B2 (en) | 1984-01-20 |
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