JPS54122790A - Culturing method of microorganisms - Google Patents
Culturing method of microorganismsInfo
- Publication number
- JPS54122790A JPS54122790A JP3065678A JP3065678A JPS54122790A JP S54122790 A JPS54122790 A JP S54122790A JP 3065678 A JP3065678 A JP 3065678A JP 3065678 A JP3065678 A JP 3065678A JP S54122790 A JPS54122790 A JP S54122790A
- Authority
- JP
- Japan
- Prior art keywords
- carrier gas
- culturing
- membrane
- medium
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
PURPOSE: To control the culturing conditions rapidly and accurately, by measuring the concentration of vaporizable components in a carrier gas diffused from the culturing medium through a hydrophobic open-cell micro-porous membrane separating the carrier gas and the medium.
CONSTITUTION: An opne-cell micro-porous flat or tubular membrane made of a hydrophobic material such as PTFE, PP, PVC, etc., is placed in a cultruing vessel. A culturing medium is brought into contact with one face of the membrane, while a carrier gas such as nitrogen, air, etc., is with the reverse side thereof. Concentrations of main carbon source or metabolized products diffused to the carrier gas through the micro-pores of the membrane, is measured. The temperature, pH value, rate of substrate flow, air flow, and dissolved oxygen concentration, etc., can be controlled to the optimum values according to the measured concentration or the rate of assimilation or production calculated therefrom. The process enables the automatic control of the culturing system, and improves the qualities and yield of the cultured product.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3065678A JPS54122790A (en) | 1978-03-16 | 1978-03-16 | Culturing method of microorganisms |
US05/961,945 US4257257A (en) | 1978-03-13 | 1978-11-20 | Method and apparatus for measuring concentrations of gaseous or volatile substances in liquids |
BR7901123A BR7901123A (en) | 1978-03-13 | 1979-02-21 | PROCESS AND APPARATUS FOR MEASURING CONCENTRATIONS OF GASEOUS OR VOLATILE SUBSTANCES IN LIQUIDS |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3065678A JPS54122790A (en) | 1978-03-16 | 1978-03-16 | Culturing method of microorganisms |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS54122790A true JPS54122790A (en) | 1979-09-22 |
JPS619032B2 JPS619032B2 (en) | 1986-03-19 |
Family
ID=12309809
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP3065678A Granted JPS54122790A (en) | 1978-03-13 | 1978-03-16 | Culturing method of microorganisms |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS54122790A (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2466503A1 (en) * | 1979-10-05 | 1981-04-10 | Intermedicat Gmbh | PROCESS FOR CULTIVATION OF ANIMAL CELLS IN THE FORM OF SUSPENDED AND MONOLAYER CROPS IN FERMENTATION CONTAINERS |
EP0052252A2 (en) * | 1980-11-18 | 1982-05-26 | Chemap AG | Method and apparatus for the submerged culture of cell cultures |
JPS5955182A (en) * | 1982-09-24 | 1984-03-30 | Denki Kagaku Keiki Co Ltd | Apparatus for determination of concentration of fermentation liquid |
JPH02240565A (en) * | 1989-03-14 | 1990-09-25 | Hitachi Ltd | Analysis, analysis apparatus, culture method, and culture device for dissolved component in culture solution |
-
1978
- 1978-03-16 JP JP3065678A patent/JPS54122790A/en active Granted
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2466503A1 (en) * | 1979-10-05 | 1981-04-10 | Intermedicat Gmbh | PROCESS FOR CULTIVATION OF ANIMAL CELLS IN THE FORM OF SUSPENDED AND MONOLAYER CROPS IN FERMENTATION CONTAINERS |
EP0052252A2 (en) * | 1980-11-18 | 1982-05-26 | Chemap AG | Method and apparatus for the submerged culture of cell cultures |
JPS5955182A (en) * | 1982-09-24 | 1984-03-30 | Denki Kagaku Keiki Co Ltd | Apparatus for determination of concentration of fermentation liquid |
JPH02240565A (en) * | 1989-03-14 | 1990-09-25 | Hitachi Ltd | Analysis, analysis apparatus, culture method, and culture device for dissolved component in culture solution |
Also Published As
Publication number | Publication date |
---|---|
JPS619032B2 (en) | 1986-03-19 |
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