JPS54113490A - Separation and purification of water-soluble enzyme - Google Patents

Separation and purification of water-soluble enzyme

Info

Publication number
JPS54113490A
JPS54113490A JP2040878A JP2040878A JPS54113490A JP S54113490 A JPS54113490 A JP S54113490A JP 2040878 A JP2040878 A JP 2040878A JP 2040878 A JP2040878 A JP 2040878A JP S54113490 A JPS54113490 A JP S54113490A
Authority
JP
Japan
Prior art keywords
water
purification
cellulose
enzyme
dehydrogenase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2040878A
Other languages
Japanese (ja)
Other versions
JPS6025115B2 (en
Inventor
Takeshi Fujita
Isamu Kokawara
Yasuo Suzuki
Katsumi Fujii
Buichi Horio
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oriental Yeast Co Ltd
Original Assignee
Oriental Yeast Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oriental Yeast Co Ltd filed Critical Oriental Yeast Co Ltd
Priority to JP53020408A priority Critical patent/JPS6025115B2/en
Publication of JPS54113490A publication Critical patent/JPS54113490A/en
Publication of JPS6025115B2 publication Critical patent/JPS6025115B2/en
Expired legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: To provide a novel and universal means for the purification of various water-soluble enzymes capable of providing high purity enzymes in the presence of a concentrated salt solution, compared with the conventional purifying means.
CONSTITUTION: An aqueous solution of a water-soluble enzyme (e.g. alcohol- dehydrogenase, glutamic acid-dehydrogenase, hexokinase, etc.) containing 0.5W 3.0 moles of a salting-out agent (e.g. ammonium sulfate, magnesium sulfate, sodium sulfate, etc.) is brought into contact with a water-insoluble polyhydroxy compound (e.g. carboxymethyl cellulose, cellulose phosphate, phosphonomethyl cellulose, etc.) to form a hydrogen bond therebetween; and the enzyme is eluted and separated from the carrier with an elutant comprising a concentrated salt solution (0.5W6.0M) containing urea.
COPYRIGHT: (C)1979,JPO&Japio
JP53020408A 1978-02-25 1978-02-25 Method for separating and purifying water-soluble enzymes Expired JPS6025115B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP53020408A JPS6025115B2 (en) 1978-02-25 1978-02-25 Method for separating and purifying water-soluble enzymes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP53020408A JPS6025115B2 (en) 1978-02-25 1978-02-25 Method for separating and purifying water-soluble enzymes

Publications (2)

Publication Number Publication Date
JPS54113490A true JPS54113490A (en) 1979-09-05
JPS6025115B2 JPS6025115B2 (en) 1985-06-17

Family

ID=12026199

Family Applications (1)

Application Number Title Priority Date Filing Date
JP53020408A Expired JPS6025115B2 (en) 1978-02-25 1978-02-25 Method for separating and purifying water-soluble enzymes

Country Status (1)

Country Link
JP (1) JPS6025115B2 (en)

Also Published As

Publication number Publication date
JPS6025115B2 (en) 1985-06-17

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