JPS54113490A - Separation and purification of water-soluble enzyme - Google Patents
Separation and purification of water-soluble enzymeInfo
- Publication number
- JPS54113490A JPS54113490A JP2040878A JP2040878A JPS54113490A JP S54113490 A JPS54113490 A JP S54113490A JP 2040878 A JP2040878 A JP 2040878A JP 2040878 A JP2040878 A JP 2040878A JP S54113490 A JPS54113490 A JP S54113490A
- Authority
- JP
- Japan
- Prior art keywords
- water
- purification
- cellulose
- enzyme
- dehydrogenase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: To provide a novel and universal means for the purification of various water-soluble enzymes capable of providing high purity enzymes in the presence of a concentrated salt solution, compared with the conventional purifying means.
CONSTITUTION: An aqueous solution of a water-soluble enzyme (e.g. alcohol- dehydrogenase, glutamic acid-dehydrogenase, hexokinase, etc.) containing 0.5W 3.0 moles of a salting-out agent (e.g. ammonium sulfate, magnesium sulfate, sodium sulfate, etc.) is brought into contact with a water-insoluble polyhydroxy compound (e.g. carboxymethyl cellulose, cellulose phosphate, phosphonomethyl cellulose, etc.) to form a hydrogen bond therebetween; and the enzyme is eluted and separated from the carrier with an elutant comprising a concentrated salt solution (0.5W6.0M) containing urea.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP53020408A JPS6025115B2 (en) | 1978-02-25 | 1978-02-25 | Method for separating and purifying water-soluble enzymes |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP53020408A JPS6025115B2 (en) | 1978-02-25 | 1978-02-25 | Method for separating and purifying water-soluble enzymes |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS54113490A true JPS54113490A (en) | 1979-09-05 |
JPS6025115B2 JPS6025115B2 (en) | 1985-06-17 |
Family
ID=12026199
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP53020408A Expired JPS6025115B2 (en) | 1978-02-25 | 1978-02-25 | Method for separating and purifying water-soluble enzymes |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6025115B2 (en) |
-
1978
- 1978-02-25 JP JP53020408A patent/JPS6025115B2/en not_active Expired
Also Published As
Publication number | Publication date |
---|---|
JPS6025115B2 (en) | 1985-06-17 |
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