JPS49110822A - - Google Patents

Info

Publication number
JPS49110822A
JPS49110822A JP49016368A JP1636874A JPS49110822A JP S49110822 A JPS49110822 A JP S49110822A JP 49016368 A JP49016368 A JP 49016368A JP 1636874 A JP1636874 A JP 1636874A JP S49110822 A JPS49110822 A JP S49110822A
Authority
JP
Japan
Prior art keywords
pathogens
sample
filter medium
concentrated
liquid filter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP49016368A
Other languages
Japanese (ja)
Other versions
JPS5642280B2 (en
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed filed Critical
Publication of JPS49110822A publication Critical patent/JPS49110822A/ja
Publication of JPS5642280B2 publication Critical patent/JPS5642280B2/ja
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/852—Klebsiella
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/873—Proteus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/874—Pseudomonas
    • Y10S435/875—Pseudomonas aeruginosa
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/882—Staphylococcus
    • Y10S435/883—Staphylococcus aureus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/885—Streptococcus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/921—Candida
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/921—Candida
    • Y10S435/922—Candida albicans

Abstract

A method and apparatus are disclosed which provide for the rapid quantitative detection of microbial pathogens in a sample fluid such as blood. Initially the pathogens are concentrated by depositing the sample fluid on a liquid filter medium such as a concentrated aqueous solution of sucrose or a microporous aqueous solution of a crosslinked polymer having a greater density than the sample, and microporous openings throughout its solubilized network (which range in size between about 1 micron and about 7 microns) and then the material is subjected to centrifugation to cause the pathogens in the sample to selectively pass into the liquid filter medium. This step not only concentrates the pathogens but also separates them from the other components of the sample such as antimicrobial constituents of blood and medicants present in blood samples, such as antibiotics. The liquid filter medium containing the concentrated pathogens is then added to nutrient media for culturing and quantitative counting.
JP1636874A 1973-02-12 1974-02-12 Expired JPS5642280B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33169373A 1973-02-12 1973-02-12
US428135*[A US3928139A (en) 1973-02-12 1974-01-09 Detection of microbial pathogens

Publications (2)

Publication Number Publication Date
JPS49110822A true JPS49110822A (en) 1974-10-22
JPS5642280B2 JPS5642280B2 (en) 1981-10-03

Family

ID=26987871

Family Applications (1)

Application Number Title Priority Date Filing Date
JP1636874A Expired JPS5642280B2 (en) 1973-02-12 1974-02-12

Country Status (7)

Country Link
US (1) US3928139A (en)
JP (1) JPS5642280B2 (en)
CA (1) CA1021238A (en)
DE (1) DE2406362A1 (en)
FR (1) FR2217419B1 (en)
GB (1) GB1458147A (en)
IT (1) IT1018621B (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5173116A (en) * 1974-12-20 1976-06-24 Wadley Res Inst & Blood Bank Shiryoryutaichunobiseibutsubyogenkinokenshutsusuruhoho oyobi kongoenshinbunrisochi
JPS5523986A (en) * 1978-06-16 1980-02-20 Boehringer Mannheim Gmbh Method and apparatus for detecting infectious bacteria in blood
WO2022208703A1 (en) * 2021-03-31 2022-10-06 株式会社日立ハイテク Method and apparatus for collecting bacteria

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3875012A (en) * 1974-01-30 1975-04-01 Wadley Res Inst & Blood Bank Apparatus and method for the detection of microbial pathogens
GB1566098A (en) * 1975-11-14 1980-04-30 Nat Res Dev Separation of solid and liquid components of mixtures
US4038150A (en) * 1976-03-24 1977-07-26 J. K. And Susie L. Wadley Research Institute And Blood Bank Sample mixing and centrifugation apparatus
US4131512A (en) * 1976-11-05 1978-12-26 J. K. And Susie L. Wadley Research Institute And Blood Bank Method for detecting microbial pathogens employing a cushioning agent
US4206282A (en) * 1977-08-29 1980-06-03 Pfizer Inc. Hypertonic culture media
US4164449A (en) * 1977-11-03 1979-08-14 J. K. And Susie L. Wadley Research Institute And Blood Bank Surface separation technique for the detection of microbial pathogens
DK366778A (en) * 1978-08-18 1980-02-19 Foss Electric As METHOD OF COUNTING BACTERIES
US4212948A (en) * 1978-10-18 1980-07-15 J. K. And Susie L. Wadley Research Institute And Blood Bank Apparatus for detecting microbial pathogens employing a cushioning agent
US4218534A (en) * 1979-02-21 1980-08-19 Dairyland Food Laboratories, Inc. Phage detection
US4634417A (en) * 1982-12-06 1987-01-06 Georgetown University Process for treatment of tumors and apparatus therefor
US4610961A (en) * 1982-12-27 1986-09-09 Eastman Kodak Company Inhibition of reduction activities of leukocytes
US4503149A (en) * 1983-06-06 1985-03-05 Conoco Inc. Method of microbial assay
USRE34405E (en) * 1983-08-01 1993-10-12 Abbott Laboratories Determination of analytes in particle-containing medium
US4581331A (en) * 1983-09-29 1986-04-08 E. I. Du Pont De Nemours And Company Method for the rapid detection of virus and viral antigens
US5171669A (en) * 1987-05-04 1992-12-15 Eastman Kodak Company Cobalt(III) reagents in combination with water soluble polymers
US4912035A (en) * 1987-06-11 1990-03-27 Eastman Kodak Company Method for minimizing interference by reductants when detecting cells in biological fluids
US5141855A (en) * 1986-07-28 1992-08-25 Eastman Kodak Company Signal amplifying cobalt (III) redox reagents and methods for the determination of analytes in aqueous fluids
US5518894A (en) * 1987-11-05 1996-05-21 Berg; James D. Rapid coliform detection system
AU2602488A (en) * 1987-11-05 1989-06-01 James D. Berg Rapid process for detecting pathogenic microorganisms
DK81389D0 (en) * 1989-02-22 1989-02-22 Foss Electric As N PROCEDURE AND APPARATUS FOR SEPARATING BACTERIES
EP0731847A1 (en) * 1993-12-03 1996-09-18 Gordon L. Dorn Method for improving quantitative recovery of microorganisms fro m specimens containing blood components
US7107824B1 (en) * 1998-01-14 2006-09-19 Johns Hopkins University Apparatus for the separation of cystic parasite forms from water
US6511819B2 (en) 1998-08-28 2003-01-28 Nye Colifast As Rapid coliform detection system
US5972641A (en) * 1998-08-28 1999-10-26 Colifast Systems Asa Rapid coliform detection system
US20030138906A1 (en) * 2001-11-05 2003-07-24 Ingun Tryland Fluorescence test for measuring heterotrophic bacteria in water
WO2010047781A2 (en) * 2008-10-20 2010-04-29 Photonic Biosystems, Inc. Filtered assay device and method
WO2010047779A2 (en) * 2008-10-20 2010-04-29 Photonic Biosystems, Inc. Integrated bioanalyzer
US20100136608A1 (en) * 2008-10-20 2010-06-03 Photonic Biosystems, Inc. Multiple Filter Array Assay
WO2010062350A1 (en) * 2008-10-31 2010-06-03 Biomerieux, Inc. Methods for detection, characterization and/or identification of microorganisms in a sealed container
CA2741008C (en) * 2008-10-31 2018-08-28 Biomerieux, Inc. Methods for separation and characterization of microorganisms using identifier agents
US10415075B2 (en) * 2008-10-31 2019-09-17 Biomerieux, Inc. Method for separation, characterization and/or identification of microorganisms using mass spectrometry
RU2510844C2 (en) * 2008-10-31 2014-04-10 Биомерьё, Инк. Container for isolation and identification of microorganism
US8647835B2 (en) 2008-10-31 2014-02-11 BIO MéRIEUX, INC. Methods for separation, characterization and/or identification of microorganisms using spectroscopy
US10059975B2 (en) * 2008-10-31 2018-08-28 Biomerieux, Inc. Methods for the isolation and identification of microorganisms
RU2541775C2 (en) * 2008-10-31 2015-02-20 Биомерьё, Инк. Method for identifying microorganisms from test blood culture sample
MX2011003982A (en) * 2008-10-31 2011-09-21 Bio Merieux Inc Methods for separation, characterization and/or identification of microorganisms using spectroscopy.
EP3050583B1 (en) * 2010-03-19 2020-01-01 Asahi Kasei Medical Co., Ltd. Cell removal system
US9181895B2 (en) 2012-03-01 2015-11-10 Johnson Controls Technology Company Start-stop retrofit systems and methods
EP3847965A1 (en) 2015-12-11 2021-07-14 Babson Diagnostics, Inc. Specimen container and method for separating serum or plasma from whole blood
CA3069984A1 (en) 2017-07-27 2019-01-31 Biomerieux, Inc. Isolation tube
WO2020223511A1 (en) * 2019-04-30 2020-11-05 SeLux Diagnostics, Inc. Rapid methods for determining microorganism growth in samples of human origin

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2706702A (en) * 1949-02-23 1955-04-19 Baltimore Biolog Lab Method for culture of specimen
US3072538A (en) * 1960-12-30 1963-01-08 Grace W R & Co Isolation of bacteria

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5173116A (en) * 1974-12-20 1976-06-24 Wadley Res Inst & Blood Bank Shiryoryutaichunobiseibutsubyogenkinokenshutsusuruhoho oyobi kongoenshinbunrisochi
JPS584554B2 (en) * 1974-12-20 1983-01-26 ジエイ ケイ アンド ス−ズイ エル ワドリ リサ−チ インステイテユ−ト アンド ブラド バンク Method and mixing centrifuge device for detecting microbial pathogens in sample fluid
JPS5523986A (en) * 1978-06-16 1980-02-20 Boehringer Mannheim Gmbh Method and apparatus for detecting infectious bacteria in blood
JPS5743238B2 (en) * 1978-06-16 1982-09-13
WO2022208703A1 (en) * 2021-03-31 2022-10-06 株式会社日立ハイテク Method and apparatus for collecting bacteria

Also Published As

Publication number Publication date
CA1021238A (en) 1977-11-22
GB1458147A (en) 1976-12-08
US3928139A (en) 1975-12-23
AU6522974A (en) 1975-08-07
FR2217419B1 (en) 1978-01-06
JPS5642280B2 (en) 1981-10-03
DE2406362A1 (en) 1974-08-15
IT1018621B (en) 1977-10-20
FR2217419A1 (en) 1974-09-06
DE2406362C2 (en) 1988-02-18

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