FR2217419A1 - - Google Patents

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Publication number
FR2217419A1
FR2217419A1 FR7404690A FR7404690A FR2217419A1 FR 2217419 A1 FR2217419 A1 FR 2217419A1 FR 7404690 A FR7404690 A FR 7404690A FR 7404690 A FR7404690 A FR 7404690A FR 2217419 A1 FR2217419 A1 FR 2217419A1
Authority
FR
France
Prior art keywords
pathogens
sample
filter medium
concentrated
liquid filter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7404690A
Other languages
French (fr)
Other versions
FR2217419B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wadley Res Inst & Blood Bank
Original Assignee
Wadley Res Inst & Blood Bank
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wadley Res Inst & Blood Bank filed Critical Wadley Res Inst & Blood Bank
Publication of FR2217419A1 publication Critical patent/FR2217419A1/fr
Application granted granted Critical
Publication of FR2217419B1 publication Critical patent/FR2217419B1/fr
Expired legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02Separating microorganisms from their culture media
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/8215Microorganisms
    • Y10S435/822Microorganisms using bacteria or actinomycetales
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/8215Microorganisms
    • Y10S435/822Microorganisms using bacteria or actinomycetales
    • Y10S435/848Escherichia
    • Y10S435/849Escherichia coli
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/8215Microorganisms
    • Y10S435/822Microorganisms using bacteria or actinomycetales
    • Y10S435/852Klebsiella
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/8215Microorganisms
    • Y10S435/822Microorganisms using bacteria or actinomycetales
    • Y10S435/873Proteus
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/8215Microorganisms
    • Y10S435/822Microorganisms using bacteria or actinomycetales
    • Y10S435/874Pseudomonas
    • Y10S435/875Pseudomonas aeruginosa
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/8215Microorganisms
    • Y10S435/822Microorganisms using bacteria or actinomycetales
    • Y10S435/882Staphylococcus
    • Y10S435/883Staphylococcus aureus
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/8215Microorganisms
    • Y10S435/822Microorganisms using bacteria or actinomycetales
    • Y10S435/885Streptococcus
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/8215Microorganisms
    • Y10S435/911Microorganisms using fungi
    • Y10S435/921Candida
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/8215Microorganisms
    • Y10S435/911Microorganisms using fungi
    • Y10S435/921Candida
    • Y10S435/922Candida albicans

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A method and apparatus are disclosed which provide for the rapid quantitative detection of microbial pathogens in a sample fluid such as blood. Initially the pathogens are concentrated by depositing the sample fluid on a liquid filter medium such as a concentrated aqueous solution of sucrose or a microporous aqueous solution of a crosslinked polymer having a greater density than the sample, and microporous openings throughout its solubilized network (which range in size between about 1 micron and about 7 microns) and then the material is subjected to centrifugation to cause the pathogens in the sample to selectively pass into the liquid filter medium. This step not only concentrates the pathogens but also separates them from the other components of the sample such as antimicrobial constituents of blood and medicants present in blood samples, such as antibiotics. The liquid filter medium containing the concentrated pathogens is then added to nutrient media for culturing and quantitative counting.
FR7404690A 1973-02-12 1974-02-12 Expired FR2217419B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33169373A 1973-02-12 1973-02-12
US428135*[A US3928139A (en) 1973-02-12 1974-01-09 Detection of microbial pathogens

Publications (2)

Publication Number Publication Date
FR2217419A1 true FR2217419A1 (en) 1974-09-06
FR2217419B1 FR2217419B1 (en) 1978-01-06

Family

ID=26987871

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7404690A Expired FR2217419B1 (en) 1973-02-12 1974-02-12

Country Status (7)

Country Link
US (1) US3928139A (en)
JP (1) JPS5642280B2 (en)
CA (1) CA1021238A (en)
DE (1) DE2406362A1 (en)
FR (1) FR2217419B1 (en)
GB (1) GB1458147A (en)
IT (1) IT1018621B (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2393063A1 (en) * 1976-11-05 1978-12-29 Wadley Res Inst & Blood Bank METHOD FOR DETERMINING PATHOGENIC MICROORGANISMS
EP0008826A2 (en) * 1978-08-18 1980-03-19 Metrofoss K/S Method and apparatus for counting bacteria in a bacteria-containing suspension, method for fluorescence staining of bacteria, and method for separating bacteria from a sample
EP0574977A1 (en) * 1987-11-05 1993-12-22 BERG, James D Direct method for detecting very low levels of coliform contamination

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3875012A (en) * 1974-01-30 1975-04-01 Wadley Res Inst & Blood Bank Apparatus and method for the detection of microbial pathogens
US3932222A (en) * 1974-12-20 1976-01-13 J. K. & Susie L. Wadley Research Institute And Blood Bank For isolating pathogenic microorganisms
GB1566098A (en) * 1975-11-14 1980-04-30 Nat Res Dev Separation of solid and liquid components of mixtures
US4038150A (en) * 1976-03-24 1977-07-26 J. K. And Susie L. Wadley Research Institute And Blood Bank Sample mixing and centrifugation apparatus
US4206282A (en) * 1977-08-29 1980-06-03 Pfizer Inc. Hypertonic culture media
US4164449A (en) * 1977-11-03 1979-08-14 J. K. And Susie L. Wadley Research Institute And Blood Bank Surface separation technique for the detection of microbial pathogens
SE452336B (en) * 1978-06-16 1987-11-23 Boehringer Mannheim Gmbh PROCEDURE FOR DISPOSAL OF DISEASE-BREATHING MICROORGANISMS WHICH ADSORBATED EXTRACORPORAL ON A SELECTIVE BINDING ADSORBENT
US4212948A (en) * 1978-10-18 1980-07-15 J. K. And Susie L. Wadley Research Institute And Blood Bank Apparatus for detecting microbial pathogens employing a cushioning agent
US4218534A (en) * 1979-02-21 1980-08-19 Dairyland Food Laboratories, Inc. Phage detection
US4634417A (en) * 1982-12-06 1987-01-06 Georgetown University Process for treatment of tumors and apparatus therefor
US4610961A (en) * 1982-12-27 1986-09-09 Eastman Kodak Company Inhibition of reduction activities of leukocytes
US4503149A (en) * 1983-06-06 1985-03-05 Conoco Inc. Method of microbial assay
USRE34405E (en) * 1983-08-01 1993-10-12 Abbott Laboratories Determination of analytes in particle-containing medium
US4581331A (en) * 1983-09-29 1986-04-08 E. I. Du Pont De Nemours And Company Method for the rapid detection of virus and viral antigens
US5171669A (en) * 1987-05-04 1992-12-15 Eastman Kodak Company Cobalt(III) reagents in combination with water soluble polymers
US5141855A (en) * 1986-07-28 1992-08-25 Eastman Kodak Company Signal amplifying cobalt (III) redox reagents and methods for the determination of analytes in aqueous fluids
US4912035A (en) * 1987-06-11 1990-03-27 Eastman Kodak Company Method for minimizing interference by reductants when detecting cells in biological fluids
US5518894A (en) * 1987-11-05 1996-05-21 Berg; James D. Rapid coliform detection system
DK81389D0 (en) * 1989-02-22 1989-02-22 Foss Electric As N PROCEDURE AND APPARATUS FOR SEPARATING BACTERIES
AU679822B2 (en) * 1993-12-03 1997-07-10 Gordon L. Dorn Method for improving quantitative recovery of microorganisms from specimens containing blood components
US7107824B1 (en) * 1998-01-14 2006-09-19 Johns Hopkins University Apparatus for the separation of cystic parasite forms from water
US6511819B2 (en) 1998-08-28 2003-01-28 Nye Colifast As Rapid coliform detection system
US5972641A (en) * 1998-08-28 1999-10-26 Colifast Systems Asa Rapid coliform detection system
US20030138906A1 (en) * 2001-11-05 2003-07-24 Ingun Tryland Fluorescence test for measuring heterotrophic bacteria in water
EP2350309A4 (en) * 2008-10-20 2012-12-05 Photonic Biosystems Inc Filtered assay device and method
EP2350308A4 (en) * 2008-10-20 2012-07-11 Photonic Biosystems Inc Multiple filter array assay
US9068976B2 (en) * 2008-10-20 2015-06-30 Photonic Biosystems Inc. Integrated filtration bioanalyzer
BRPI0919896A2 (en) * 2008-10-31 2016-02-16 Bio Merieux Inc methods for the separation, characterization and / or identification of microorganisms by mass spectroscopy.
EP2364218B1 (en) * 2008-10-31 2020-04-15 Biomerieux, Inc Container for the isolation and identification of microorganisms
CN102272601B (en) * 2008-10-31 2014-09-17 生物梅里埃公司 Methods for separation and characterization of microorganisms using identifier agents
MX2011004106A (en) * 2008-10-31 2011-08-15 Bio Merieux Inc Methods for separation, characterization, and/or identification of microorganisms using raman spectroscopy.
EP2364447B1 (en) * 2008-10-31 2019-06-12 Biomerieux, Inc Method for the identification of microorganisms
CA2740836C (en) * 2008-10-31 2017-03-21 Biomerieux, Inc. Methods for separation, characterization and/or identification of microorganisms using spectroscopy
US10167494B2 (en) * 2008-10-31 2019-01-01 Biomerieux, Inc. Method for detection, characterization and/or identification of microorganisms in a sealed container
US8647835B2 (en) 2008-10-31 2014-02-11 BIO MéRIEUX, INC. Methods for separation, characterization and/or identification of microorganisms using spectroscopy
ES2547436T3 (en) 2010-03-19 2015-10-06 Asahi Kasei Medical Co., Ltd. Cell Extraction Procedure
US9181895B2 (en) 2012-03-01 2015-11-10 Johnson Controls Technology Company Start-stop retrofit systems and methods
CN113751095B (en) 2015-12-11 2024-01-09 巴布森诊断公司 Sample container and method for separating serum or plasma from whole blood
US11090646B2 (en) 2017-07-27 2021-08-17 Biomerieux, Inc. Isolation tube
US20200347432A1 (en) * 2019-04-30 2020-11-05 SeLux Diagnostics, Inc. Rapid methods for determining microorganism growth in samples of human origin
WO2022208703A1 (en) * 2021-03-31 2022-10-06 株式会社日立ハイテク Method and apparatus for collecting bacteria
US12050052B1 (en) 2021-08-06 2024-07-30 Babson Diagnostics, Inc. Refrigerated carrier device for biological samples
US12025629B2 (en) 2022-04-06 2024-07-02 Babson Diagnostics, Inc. Automated centrifuge loader

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3072538A (en) * 1960-12-30 1963-01-08 Grace W R & Co Isolation of bacteria

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2706702A (en) * 1949-02-23 1955-04-19 Baltimore Biolog Lab Method for culture of specimen

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3072538A (en) * 1960-12-30 1963-01-08 Grace W R & Co Isolation of bacteria

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2393063A1 (en) * 1976-11-05 1978-12-29 Wadley Res Inst & Blood Bank METHOD FOR DETERMINING PATHOGENIC MICROORGANISMS
EP0008826A2 (en) * 1978-08-18 1980-03-19 Metrofoss K/S Method and apparatus for counting bacteria in a bacteria-containing suspension, method for fluorescence staining of bacteria, and method for separating bacteria from a sample
EP0008826A3 (en) * 1978-08-18 1980-09-03 Metrofoss K/S Method and apparatus for counting bacteria in a bacteria-containing suspension, method for fluorescence staining of bacteria, and method for separating bacteria from a sample
EP0574977A1 (en) * 1987-11-05 1993-12-22 BERG, James D Direct method for detecting very low levels of coliform contamination

Also Published As

Publication number Publication date
JPS5642280B2 (en) 1981-10-03
FR2217419B1 (en) 1978-01-06
CA1021238A (en) 1977-11-22
DE2406362A1 (en) 1974-08-15
JPS49110822A (en) 1974-10-22
AU6522974A (en) 1975-08-07
DE2406362C2 (en) 1988-02-18
GB1458147A (en) 1976-12-08
US3928139A (en) 1975-12-23
IT1018621B (en) 1977-10-20

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