JPH114680A - Bacteria decomposing polylactic acid resin and microbial degradation of polylactic acid resin - Google Patents
Bacteria decomposing polylactic acid resin and microbial degradation of polylactic acid resinInfo
- Publication number
- JPH114680A JPH114680A JP16023097A JP16023097A JPH114680A JP H114680 A JPH114680 A JP H114680A JP 16023097 A JP16023097 A JP 16023097A JP 16023097 A JP16023097 A JP 16023097A JP H114680 A JPH114680 A JP H114680A
- Authority
- JP
- Japan
- Prior art keywords
- polylactic acid
- acid resin
- ferm
- decomposing
- bacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J11/00—Recovery or working-up of waste materials
- C08J11/04—Recovery or working-up of waste materials of polymers
- C08J11/10—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
- C08J11/105—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with enzymes
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
- C08J2367/04—Polyesters derived from hydroxy carboxylic acids, e.g. lactones
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/50—Reuse, recycling or recovery technologies
- Y02W30/62—Plastics recycling; Rubber recycling
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- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biological Depolymerization Polymers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Processing Of Solid Wastes (AREA)
- Manufacture Of Porous Articles, And Recovery And Treatment Of Waste Products (AREA)
- Separation, Recovery Or Treatment Of Waste Materials Containing Plastics (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、新規な生物学的処
理法によるポリ乳酸樹脂の分解方法および分解能を有す
る耐熱性バクテリアに関する。TECHNICAL FIELD The present invention relates to a method for decomposing a polylactic acid resin by a novel biological treatment method and a heat-resistant bacterium having a resolution.
【０００２】[0002]
【従来の技術】最近、プラスチック廃棄物の処理が問題
になっている。処理法としては焼却や埋め立てが主であ
るが、焼却は地球温暖化の促進、埋め立ては埋め立て地
の減少等の問題を抱え、生物学的分解処理法が注目され
ている。また、ポリ乳酸樹脂は次世代のプラスチックと
して種々の用途開発が進められており、近い将来、現在
使用されているプラスチック同様、廃棄物問題がクロー
ズアップされることが十分に予想される。2. Description of the Related Art Recently, the treatment of plastic waste has become a problem. The main treatment methods are incineration and landfill, but incineration has problems such as promotion of global warming and landfill has problems such as reduction of landfill sites. In addition, polylactic acid resins are being developed for various applications as next-generation plastics, and it is fully expected that in the near future, as with plastics currently used, waste problems will be highlighted.
【０００３】ポリ乳酸樹脂は水系の中で加水分解する高
分子であり、現在医療や医薬用材料として応用されてい
るが、澱粉等の再生可能な資源から乳酸醗酵を通して合
成できることから、環境分解が困難である汎用プラスチ
ックに代わる生分解性プラスチックの素材として注目さ
れている。ポリ乳酸樹脂は、その構成モノマーの種類に
よりポリＬ−乳酸、ポリＤ−乳酸、ポリＤＬ−乳酸ある
いは、他の高分子との共重合体が存在している。[0003] Polylactic acid resin is a polymer that hydrolyzes in an aqueous system, and is currently used as a medical or pharmaceutical material. However, since it can be synthesized from renewable resources such as starch through lactic acid fermentation, environmental degradation occurs. It is attracting attention as a material for biodegradable plastics that can replace difficult general-purpose plastics. The polylactic acid resin includes poly (L-lactic acid), poly (D-lactic acid), poly (DL-lactic acid), or a copolymer with another polymer depending on the type of the constituent monomer.
【０００４】[0004]
【発明が解決しようとする課題】ポリ乳酸樹脂は酵素に
よって加水分解が促進されると知られている。しかしな
がら、これまでポリ乳酸樹脂およびその廃棄物を直接生
物学的に分解処理するための微生物およびその微生物に
よる分解法技術は、ほとんど知られておらず、本件発明
者による放線菌Amycolatopsis mediterranei（ＦＥＲＭ
Ｐ−１４９２１）およびこの菌を用いた分解などに限
られていた。It is known that hydrolysis of polylactic acid resins is promoted by enzymes. However, a microorganism for directly biologically decomposing a polylactic acid resin and its waste and a technique for decomposing the microorganism by the microorganism have been scarcely known, and the actinomycetes Amycolatopsis mediterranei (FERM) by the present inventors have not been known so far.
P-14921) and degradation using this bacterium.
【０００５】そこで、本発明は、ポリ乳酸樹脂およびそ
れらを含むプラスチックを、直接生物学的に分解処理す
るバクテリアおよびその方法を提供する事を目的とす
る。Accordingly, an object of the present invention is to provide a bacterium which directly biologically degrades a polylactic acid resin and a plastic containing the same, and a method therefor.
【０００６】[0006]
【課題を解決するための手段】本発明者らは、前記課題
を解決するべく鋭意研究を重ねた結果、微生物学的手段
により優れたポリ乳酸分解活性を有するバクテリアを見
出し、本研究を完成するに至った。Means for Solving the Problems The present inventors have conducted intensive studies to solve the above-mentioned problems, and as a result, found a bacterium having excellent polylactic acid decomposing activity by microbiological means, and completed this research. Reached.
【０００７】即ち、本発明によれば、ポリ乳酸をバクテ
リアで分解する事を特徴とするポリ乳酸樹脂の分解方法
が提供され、また無機塩類を含む培地にポリ乳酸樹脂と
Bacillus属に属するバクテリアを添加し分解する事を特
徴とするポリ乳酸樹脂の分解方法が提供され、特に前記
Bacillus属に属するバクテリア（ＦＥＲＭ Ｐ−１６１
８１、ＦＥＲＭ Ｐ−１６１８２、ＦＥＲＭ Ｐ−１６
１８３）である事を特徴とする前記ポリ乳酸樹脂の分解
方法が提供される。更に、培養条件がｐＨ４．０〜１
０．０、温度１０〜７５℃である事を特徴とする前記ポ
リ乳酸樹脂の分解方法が提供される。That is, according to the present invention, there is provided a method for decomposing a polylactic acid resin, which comprises decomposing polylactic acid with bacteria, and a method for dissolving polylactic acid in a medium containing inorganic salts.
A method for decomposing a polylactic acid resin is provided, which comprises adding and decomposing bacteria belonging to the genus Bacillus.
Bacterium belonging to the genus Bacillus (FERM P-161)
81, FERM P-16182, FERM P-16
183), wherein the method for decomposing the polylactic acid resin is provided. Furthermore, when the culture conditions are pH 4.0-1
0.0, and a temperature of 10 to 75 ° C. is provided.
【０００８】なお、本発明でいうポリ乳酸とは、乳酸を
主要成分とする重合体を指し、ポリＬ−乳酸やポリＤ−
乳酸等のポリ乳酸ホモポリマー、ポリＬ／Ｄ−乳酸共重
合体、およびこれらに他のポリマーを共重合させたポリ
乳酸共重合体、そして上記ポリマー間、および他の成分
ポリマーとのブレンド体を含み、重合体中の乳酸成分の
重量比率が１０％以上のものを言う。The term "polylactic acid" as used in the present invention refers to a polymer containing lactic acid as a main component, such as poly-L-lactic acid and poly-D-lactic acid.
Polylactic acid homopolymers such as lactic acid, poly L / D-lactic acid copolymers, polylactic acid copolymers obtained by copolymerizing these with other polymers, and blends between the above polymers and other component polymers. It refers to those containing 10% or more by weight of the lactic acid component in the polymer.
【０００９】本発明は、ポリ乳酸樹脂の分解を、その分
解能を有するバクテリアに行わせる事で、好気条件下で
のポリ乳酸樹脂の分解処理を可能にするものである。According to the present invention, a polylactic acid resin is decomposed by a bacterium having the ability to decompose the polylactic acid resin, whereby the polylactic acid resin can be decomposed under aerobic conditions.
【００１０】ポリ乳酸分解活性を有する微生物はバクテ
リアである。その中で特に Bacillus 属に属するバクテ
リアが好ましく、その分離獲得は以下に示す方法により
行った。本発明者らは、茨城県つくば市の土壌およびコ
ンポストを採用し、以下に詳述する操作を経て、ポリ乳
酸樹脂を分解する好気性微生物を分離獲得した。Microorganisms having polylactic acid degrading activity are bacteria. Among them, a bacterium belonging to the genus Bacillus is particularly preferred, and its isolation and acquisition were performed by the following method. The present inventors employed soil and compost from Tsukuba City, Ibaraki Prefecture, and obtained and isolated aerobic microorganisms that degrade polylactic acid resin through the operations described in detail below.
【００１１】以下の表１に示す基本培地１Ｌに１０００
mgのポリ乳酸樹脂を乳化させ、１．５％の寒天を含む寒
天平板培地を調製した。各サンプル１g を５mlの滅菌水
に懸濁させ、１０〜１０2 に希釈した後、０．２mlを調
製した培地に塗布した。培養は、５０℃の孵卵機中で行
った。培地上に生育したコロニーの中で、コロニーの周
囲に透明領域を形成したものを、ポリ乳酸樹脂の分解菌
とし、白金耳でコロニーを釣り上げる事により単離操作
を行った。[0011] One liter of the basic medium shown in Table 1
mg of polylactic acid resin was emulsified to prepare an agar plate medium containing 1.5% agar. Each sample 1g was suspended in sterile water 5 ml, diluted to 10 to 10 2, was applied to a medium to prepare a 0.2 ml. Culture was performed in an incubator at 50 ° C. Among the colonies grown on the medium, those having a transparent region formed around the colonies were used as polylactic acid resin-degrading bacteria, and the colonies were picked up with a platinum loop to perform an isolation operation.
【００１２】[0012]
【表１】 基本培地組成 Ｎａ２ＭｏＯ４・２Ｈ２Ｏ ０．５mg Ｎａ２ＷＯ４・２Ｈ２Ｏ ０．５mg ＭｎＳＯ４ ０．５mg ＦｅＳＯ４・７Ｈ２Ｏ １０．０mg ＮａＣｌ １０．０mg （ＮＨ４）２ＳＯ４ １０００．０mg ＭｇＳＯ４・７Ｈ２Ｏ ２００．０mg Ｋ２ＨＰＯ４ １６００．０mg ＫＨ２ＰＯ４ ２００．０mg 界面活性剤 １００．０mg 酵母エキス １００．０mg 蒸留水１Ｌ中 ｐＨ７．０ 培地中生育したコロニーの中から、周囲に透明領域を確
認したサンプルのコロニーを白金耳で釣り上げ、同様な
培地を用い純粋分離し、ポリ乳酸樹脂分解菌（ＦＥＲＭ
Ｐ−１６１８１、ＦＥＲＭ Ｐ−１６１８２、ＦＥＲ
Ｍ Ｐ−１６１８３）を得る事が出来た。Table 1 Basic medium composition Na2MoO4.2H2O 0.5mg Na2WO4.2H2O 0.5mg MnSO4 0.5mg FeSO4.7H2O 10.0mg NaCl 10.0mg (NH4) 2SO4 1000.0mg MgSO4.7H2O 200.0mg K2HPO4 1600.0mg KH2PO4 200.0mg Surfactant 100.0mg Yeast extract 100.0mg From colonies grown in pH 7.0 medium in 1 L of distilled water , colonies of a sample having a transparent area around the colonies were picked up with a platinum loop, and the same medium was used. Pure lactic acid resin degrading bacteria (FERM)
P-16181, FERM P-16182, FER
MP-16183) could be obtained.
【００１３】分解菌株を、ＮＵＴＲＩＥＮＴ ＢＲＯＴ
Ｈに接種しコロニーを形成させ、得られた菌体の性状に
ついて顕微鏡で観察した。結果は以下の表２、表３及び
表４に示す。[0013] The degrading bacterial strain is called NUTRIENT BROT.
H was inoculated to form a colony, and the properties of the obtained cells were observed with a microscope. The results are shown in Tables 2, 3 and 4 below.
【００１４】[0014]
【表２】 菌株名 Bacillus （ＦＥＲＭ Ｐ−１６１８１） コロニーの形態 不規則 直径３〜４ｍｍ グラム染色 variable 胞子 ＋ 移動性 − 生育温度 ４５℃ ＋ ５０℃ ＋ ５５℃ ＋ ６０℃ − ６５℃ − カタラーゼ ＋ オキシダーゼ − ＯＦテスト Ｎ．Ｄ． N.D.＝Not Determined [Table 2] Strain name Bacillus (FERM P-16181) Morphology of colonies Irregular 3-4 mm diameter Gram stain variable Spores + Mobility-Growth temperature 45 ° C + 50 ° C + 55 ° C + 60 ° C-65 ° C-Catalase + Oxidase- OF test N . D. ND = Not Determined
【表３】 菌株名 Bacillus （ＦＥＲＭ Ｐ−１６１８２） コロニーの形態 不規則 直径２ｍｍ グラム染色 variable 胞子 ＋ 移動性 − 生育温度 ４５℃ ＋ ５０℃ ＋ ５５℃ ＋ ６０℃ ＋ カタラーゼ ＋ オキシダーゼ − ＯＦテスト Ｎ．Ｄ． N.D.＝Not Determined [Table 3] Strain name Bacillus (FERM P-16182) Morphology of colonies Irregular diameter 2 mm Gram stain variable Spores + Mobility-Growth temperature 45 ° C + 50 ° C + 55 ° C + 60 ° C + Catalase + Oxidase- OF test D. ND = Not Determined
【表４】 菌株名 Bacillus （ＦＥＲＭ Ｐ−１６１８３） コロニーの形態 不規則 直径３ｍｍ グラム染色 ＋ 胞子 ＋ 移動性 − 生育温度 ４５℃ ＋ ５０℃ ＋ ５５℃ ＋ ６０℃ ＋ ６５℃ ＋ カタラーゼ ＋ オキシダーゼ − ＯＦテスト Ｎ．Ｄ． N.D.＝Not Determined 表２、表３及び表４に示す結果等を Bergey's Manual o
f Determinative Bacteriology ９版等に参照したとこ
ろ、上記の菌株はBacillus 属の菌と性状が類似してい
る事から、ＦＥＲＭ Ｐ−１６１８１は Bacillus sub
tilis 、ＦＥＲＭ Ｐ−１６１８２は Bacillus circu
lans、ＦＥＲＭ Ｐ−１６１８３は Bacillus stearot
hermophilus である事が示された。[Table 4] Strain name Bacillus (FERM P-16183) Morphology of colonies Irregular 3 mm diameter Gram stain + Spores + Mobility-Growth temperature 45 ° C + 50 ° C + 55 ° C + 60 ° C + 65 ° C + Catalase + Oxidase- OF test D. ND = Not Determined The results shown in Table 2, Table 3 and Table 4 were taken from Bergey's Manual o
f Reference to the 9th edition of Determinative Bacteriology shows that the above strains have similar properties to those of the genus Bacillus.
tilis, FERM P-16182 is from Bacillus circu
lans, FERM P-16183 is Bacillus stearot
hermophilus.
【００１５】本発明で使用される菌株は Bacillus 属と
し、ポリ乳酸樹脂を処理するために本菌株群（ＦＥＲＭ
Ｐ−１６１８１、ＦＥＲＭ Ｐ−１６１８２およびＦ
ＥＲＭ Ｐ−１６１８３）を含んだ微生物群を用いる事
が望ましい。The strain used in the present invention is of the genus Bacillus, and is used to treat polylactic acid resin.
P-16181, FERM P-16182 and F
It is desirable to use a microorganism group containing ERM P-16183).
【００１６】本菌株または、本菌株を含む微生物群は必
要に応じて、凍結乾燥した粉末、その粉末と各種ビタミ
ンやミネラルと必要な栄養源を配合した後に打錠した錠
剤、先に記した基本培地中で生育培養させた培養液等の
形で、ポリ乳酸樹脂の処理に提供される。The strain or a microorganism group containing the strain may be freeze-dried powder, a tablet prepared by blending the powder with various vitamins and minerals and necessary nutrients, and then compressed as necessary. It is provided for the treatment of polylactic acid resin in the form of a culture solution or the like grown and cultured in a medium.
【００１７】本研究における培養に於いて使用される基
本培地は、窒素源として例えば、硫酸アンモニウム、リ
ン酸アンモニウム、炭酸アンモニウム等が使用され、そ
の他無機塩としてリン酸一カリウム、リン酸二カリウ
ム、硫酸マグネシウム、塩化ナトリウム、硫酸第一鉄、
モリブテン酸ナトリウム、タングステン酸ナトリウムお
よび硫酸マンガン等の、通常利用される培養源が使用さ
れ、そのｐＨは４．０〜１０．０であり、好ましくはｐ
Ｈ５．０〜８．０である。また、培養温度は１０〜７５
℃であり、好ましくは３０〜７０℃である。The basal medium used in the culture in this study uses, for example, ammonium sulfate, ammonium phosphate, ammonium carbonate, etc. as a nitrogen source, and monopotassium phosphate, dipotassium phosphate, and sulfate as other inorganic salts. Magnesium, sodium chloride, ferrous sulfate,
Commonly used culture sources, such as sodium molybdate, sodium tungstate and manganese sulfate, are used and have a pH of 4.0 to 10.0, preferably p
H is 5.0 to 8.0. The culture temperature is 10 to 75.
° C, preferably 30 to 70 ° C.
【００１８】本発明のポリ乳酸の生物学的分解処理は、
培養槽に先に示した基本培地、処理されるべきポリ乳酸
樹脂、上記菌株および菌群を配合した粉末、錠剤、培養
液を添加する事で行われる事が望ましいが、上記菌株を
活性汚泥およびコンポストに組み込んでも良い。なお、
基本培地に対するポリ乳酸樹脂の投入量は、０．０１重
量％〜１０．０重量％が望ましい。添加する微生物量は
極少量であっても構わないが、投入量が処理時間に影響
を及ぼさないためにポリ乳酸樹脂に対して、０．０１重
量％以上が好ましい。The biological decomposition treatment of the polylactic acid of the present invention comprises
The basic medium shown in the culture tank, the polylactic acid resin to be treated, the powder containing the above strains and bacterial groups, tablets, it is desirable to perform by adding a culture solution, the above strain is activated sludge and It may be incorporated in compost. In addition,
The amount of the polylactic acid resin to be added to the basic medium is preferably 0.01% by weight to 10.0% by weight. The amount of microorganisms to be added may be extremely small, but is preferably 0.01% by weight or more based on the polylactic acid resin, since the amount of addition does not affect the processing time.
【００１９】[0019]
（実験例１）表１の基本培地１Ｌに１０００mgのポリ乳
酸樹脂（Mw : 1.89 ×105 ）を乳化させた１．５％の寒
天を含む寒天平板培地を用意し、ＦＥＲＭ Ｐ−１６１
８１菌株を接種し、５０℃で２週間培養した。その結果
は図１に示したように、乳化白濁した寒天平板培地上で
の、ＦＥＲＭ Ｐ−１６１８１菌株のコロニー形成に伴
い、コロニー周囲に透明領域が確認された。(Experimental Example 1) An agar plate medium containing 1.5% agar in which 1000 mg of polylactic acid resin (Mw: 1.89 × 10 5 ) was emulsified in 1 L of the basic medium shown in Table 1 was prepared, and FERM P-161 was prepared.
81 strains were inoculated and cultured at 50 ° C. for 2 weeks. As a result, as shown in FIG. 1, a transparent region was confirmed around the colony along with the colony formation of the FERM P-16181 strain on the emulsified cloudy agar plate medium.
【００２０】（実験例２）表１の基本培地１Ｌに１００
０mgのポリ乳酸樹脂（Mw : 1.89 ×105 ）を乳化させた
１．５％の寒天を含む寒天平板培地を用意し、ＦＥＲＭ
Ｐ−１６１８２菌株を接種し、５０℃で２週間培養し
た。その結果は図２に示したように、乳化白濁した寒天
平板培地上での、ＦＥＲＭ Ｐ−１６１８２菌株のコロ
ニー形成に伴い、コロニー周囲に透明領域が確認され
た。(Experimental Example 2) 100 L was added to 1 L of the basic medium shown in Table 1.
An agar plate medium containing 1.5% agar emulsified with 0 mg of a polylactic acid resin (Mw: 1.89 × 10 5 ) was prepared, and FERM was prepared.
P-16182 strain was inoculated and cultured at 50 ° C. for 2 weeks. As a result, as shown in FIG. 2, a transparent region was confirmed around the colony along with the colony formation of the FERM P-16182 strain on the emulsified cloudy agar plate medium.
【００２１】（実験例３）表１の基本培地１Ｌに１００
０mgのポリ乳酸樹脂（Mw : 1.89 ×105 ）を乳化させた
１．５％の寒天を含む寒天平板培地を用意し、ＦＥＲＭ
Ｐ−１６１８３菌株を接種し、５０℃で２週間培養し
た。その結果は図３に示したように、乳化白濁した寒天
平板培地上での、ＦＥＲＭ Ｐ−１６１８３菌株のコロ
ニー形成に伴い、コロニー周囲に透明領域が確認され
た。(Experimental Example 3) 100 L was added to 1 L of the basic medium shown in Table 1.
An agar plate medium containing 1.5% agar emulsified with 0 mg of a polylactic acid resin (Mw: 1.89 × 10 5 ) was prepared, and FERM was prepared.
P-16183 strain was inoculated and cultured at 50 ° C. for 2 weeks. As a result, as shown in FIG. 3, a transparent region was confirmed around the colony along with the colony formation of the FERM P-16183 strain on the emulsified cloudy agar plate medium.
【００２２】（実験例４）表１の基本培地１００mlに対
し、粉末加工したポリ乳酸樹脂（Mw : 1.89 ×105 ）を
炭素源として１００mg添加したものを用意し、ＦＥＲＭ
Ｐ−１６１８３菌株を接種し、５０℃で、粉末加工し
たポリ乳酸樹脂を４週間、１８０ｒｐｍ回転型振とう機
で培養した。添加した粉末加工ポリ乳酸樹脂の分解に伴
う、ポリ乳酸樹脂の回収重量（クロロホルム抽出）の変
化を測定した。その結果は表５に示したように、菌株を
植菌しないコントロールが培養前後で重量が変化しなか
ったのに比べ、ポリ乳酸樹脂の回収重量が約７％減少し
た。(Experimental Example 4) To 100 ml of the basic medium shown in Table 1, 100 mg of a powdered polylactic acid resin (Mw: 1.89 × 10 5 ) was added as a carbon source, and FERM was prepared.
The P-16183 strain was inoculated, and the powdered polylactic acid resin was cultured at 50 ° C. for 4 weeks on a rotary shaker at 180 rpm. The change in the recovered weight (extracted with chloroform) of the polylactic acid resin accompanying the decomposition of the added powdered polylactic acid resin was measured. The results, as shown in Table 5, showed that the weight of the polylactic acid resin recovered was reduced by about 7% as compared with the control without inoculation of the strain, the weight of which did not change before and after the culture.
【００２３】[0023]
【表５】 FERM P-16183菌株による分解に伴うポリ乳酸樹脂の回収重量の変化 回収重量（mg） 培養前 培養後 FERM P-16183 無植菌 100.0 100.0 FERM P-16183 植菌 100.0 93.5 以上の事から、分離菌株は高分子のポリ乳酸樹脂を分解
出来る事が明らかとなった。[Table 5] Change in recovered weight of polylactic acid resin due to degradation by FERM P-16183 strain Recovered weight (mg) Before culturing After culturing FERM P-16183 Non -inoculated 100.0 100.0 FERM P-16183 Inoculated 100.0 93.5 or more From the results, it was clarified that the isolated strain was able to degrade the high molecular weight polylactic acid resin.
【００２４】[0024]
【発明の効果】本発明のポリ乳酸樹脂の分解方法は、ポ
リ乳酸樹脂廃棄物の処理方法であり、これまで既存の焼
却のように排ガスも生じず、埋立処理に比べて極めて省
時間な技術であり、廃棄物処理上で極めて価値の高い方
法である。The method for decomposing a polylactic acid resin according to the present invention is a method for treating polylactic acid resin waste, which does not generate exhaust gas unlike conventional incineration, and is a very time-saving technique compared to landfill treatment. This is an extremely valuable method of treating waste.
【００２５】また、コンポスト化施設で本発明の処理方
法を用いる事により、ポリ乳酸樹脂を有機酸等の有用物
質や堆肥に転換する事も可能である。Further, by using the treatment method of the present invention in a composting facility, it is possible to convert polylactic acid resin into useful substances such as organic acids and compost.
【図１】ＦＥＲＭ Ｐ−１６１８１による寒天平板培地
中のポリ乳酸樹脂を分解しているコロニーの培養２週間
後の状態を示す顕微鏡写真。FIG. 1 is a photomicrograph showing the state of a colony degrading a polylactic acid resin in an agar plate medium by FERM P-16181 after 2 weeks of culture.
【図２】ＦＥＲＭ Ｐ−１６１８２による寒天平板培地
中のポリ乳酸樹脂を分解しているコロニーの培養２週間
後の状態を示す顕微鏡写真。FIG. 2 is a micrograph showing the state of a colony degrading a polylactic acid resin in an agar plate medium by FERM P-16182 after 2 weeks of culture.
【図３】ＦＥＲＭ Ｐ−１６１８３による寒天平板培地
中のポリ乳酸樹脂を分解しているコロニーの培養２週間
後の状態を示す顕微鏡写真。FIG. 3 is a micrograph showing the state of a colony degrading a polylactic acid resin in an agar plate medium using FERM P-16183 after 2 weeks of culture.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.6 識別記号 ＦＩ //(Ｃ１２Ｎ 1/20 Ｃ１２Ｒ 1:125） (Ｃ１２Ｎ 1/20 Ｃ１２Ｒ 1:09） (Ｃ１２Ｎ 1/20 Ｃ１２Ｒ 1:07） (72)発明者 長井 直子 京都市中京区西ノ京桑原町１番地 株式会 社島津製作所三条工場内 (72)発明者 軸屋 博之 茨城県つくば市吾妻３丁目17−１ 株式会 社島津製作所つくば支店内──────────────────────────────────────────────────続 き Continued on front page (51) Int.Cl. 6 Identification symbol FI // (C12N 1/20 C12R 1: 125) (C12N 1/20 C12R 1:09) (C12N 1/20 C12R 1:07) (72) Inventor Naoko Nagai 1 Nishinokyo Kuwabara-cho, Nakagyo-ku, Kyoto City Shimazu Corporation Sanjo Plant (72) Inventor Hiroyuki Ashiya 3-171-1 Azuma Azuma Tsukuba City, Ibaraki Prefecture Tsukuba Branch Shimadzu Corporation
Claims (6)
s subtilis （ＦＥＲＭ Ｐ−１６１８１）。1. Bacillu having the resolution of polylactic acid resin
s subtilis (FERM P-16181).
s circulans（ＦＥＲＭ Ｐ−１６１８２）。2. Bacillu having the resolution of polylactic acid resin
s circulans (FERM P-16182).
s stearothermophilus （ＦＥＲＭ Ｐ−１６１８
３）。3. Bacillu having the resolution of polylactic acid resin
s stearothermophilus (FERM P-1618
3).
分解する事を特徴とするポリ乳酸樹脂の分解方法。4. A method for decomposing a polylactic acid resin, comprising decomposing the polylactic acid resin with a bacterium belonging to the genus Bacillus.
tilis 、 Bacilluscirculans、 Bacillus stearotherm
ophilus である請求項４記載のポリ乳酸樹脂の分解方
法。5. The bacterium according to claim 4, wherein the bacterium is Bacillus sub.
tilis, Bacilluscirculans, Bacillus stearotherm
The method for decomposing a polylactic acid resin according to claim 4, which is ophilus.
us属に属する菌である請求項４記載のポリ乳酸樹脂の分
解方法。6. The method of claim 4, wherein the bacterium is heat-resistant Bacill.
The method for decomposing a polylactic acid resin according to claim 4, which is a bacterium belonging to the genus us.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16023097A JP3734118B2 (en) | 1997-06-17 | 1997-06-17 | Decomposition method of polylactic acid resin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16023097A JP3734118B2 (en) | 1997-06-17 | 1997-06-17 | Decomposition method of polylactic acid resin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH114680A true JPH114680A (en) | 1999-01-12 |
| JP3734118B2 JP3734118B2 (en) | 2006-01-11 |
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ID=15710527
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP16023097A Expired - Lifetime JP3734118B2 (en) | 1997-06-17 | 1997-06-17 | Decomposition method of polylactic acid resin |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007319077A (en) * | 2006-05-31 | 2007-12-13 | National Univ Corp Shizuoka Univ | Method for degrading plastic and microorganism |
| JP2007319078A (en) * | 2006-05-31 | 2007-12-13 | National Univ Corp Shizuoka Univ | Method for degrading polylactic acid and microorganism |
| JP2008167701A (en) * | 2007-01-12 | 2008-07-24 | National Univ Corp Shizuoka Univ | Method for decomposing polylactic acid, polylactic acid-decomposing composition and microorganism used therefor |
| JP2009154125A (en) * | 2007-12-27 | 2009-07-16 | Osaka Gas Co Ltd | Method of solubilizing polylactic acid and biologically gasifying method |
| WO2022123421A1 (en) * | 2020-12-09 | 2022-06-16 | Innovazione E Sviluppo Sostenibile S.r.l. | Plastic-degrading solution |
-
1997
- 1997-06-17 JP JP16023097A patent/JP3734118B2/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007319077A (en) * | 2006-05-31 | 2007-12-13 | National Univ Corp Shizuoka Univ | Method for degrading plastic and microorganism |
| JP2007319078A (en) * | 2006-05-31 | 2007-12-13 | National Univ Corp Shizuoka Univ | Method for degrading polylactic acid and microorganism |
| JP2008167701A (en) * | 2007-01-12 | 2008-07-24 | National Univ Corp Shizuoka Univ | Method for decomposing polylactic acid, polylactic acid-decomposing composition and microorganism used therefor |
| JP2009154125A (en) * | 2007-12-27 | 2009-07-16 | Osaka Gas Co Ltd | Method of solubilizing polylactic acid and biologically gasifying method |
| WO2022123421A1 (en) * | 2020-12-09 | 2022-06-16 | Innovazione E Sviluppo Sostenibile S.r.l. | Plastic-degrading solution |
Also Published As
| Publication number | Publication date |
|---|---|
| JP3734118B2 (en) | 2006-01-11 |
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